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Issue: Vol. 4483 No. 3: 24 Sept. 2018
Type: Article
Published: 2018-09-24
DOI: 10.11646/zootaxa.4483.3.1
Page range: 401–427
Abstract views: 470
PDF downloaded: 9

A phylogenetic approach to the Philippines endemic centipedes of the genus Scolopendra Linnaeus, 1758 (Scolopendromorpha, Scolopendridae), with the description of a new species

Departament de Ciències Ambientals i Recursos Naturals, Universitat d’Alacant. Carretera de Sant Vicent del Raspeig s/n C.P. 03690, San Vicent del Raspeig, Alacant (Spain)
Laboratorio de Genética Molecular, Hospital General Universitario de Elche, Instituto de Investigación Sanitaria y Biomédica de Alicante (ISABIAL—Fundación FISABIO). Camí de l'Almazara, 11, C.P. 03203 Elche, Alicante, (Spain)
Centro de Investigaciones Biológicas C.I.B. (CSIC). Ramiro de Maeztu, 9, C.P: 28040-Madrid (Spain)
Myriapoda Chilopoda scolopendromorph paradoxa spinosissima subcrustalis barcode cytochrome oxidase I

Abstract

The genus Scolopendra Linnaeus, 1758 is represented in the Philippines’ fauna by five species, two of which are endemic. Mitochondrial DNA sequences of gene cytochrome c oxidase subunit I (COI) were obtained from six Scolopendra specimens belonging to two endemic species and a new one, described here as Scolopendra paradoxa Doménech sp. nov. These sequences were analyzed together with another forty-one sequences from GenBank, including additional species of Scolopendra and a few representatives of other Scolopendridae genera. Phylogenetic trees inferred from the COI analysis using maximum likelihood and neighbor joining showed the three Philippines Scolopendra endemic species as a polyphyletic group coherent with their respective morphologies, although the position of S. spinosissima Kraepelin, 1903 varied within the obtained trees. Species delimitation based on standard external morphological characters was also concordant with the observed genetic distances, monophyly and node support, confirming S. subcrustalis Kronmüller, 2009 and S. paradoxa sp. nov. as separate species also at the molecular level, while only the position of S. spinosissima could not be properly established with any of the statistical methods used. In addition, the male genitalia of the three studied species were found to lack gonopods and a penis. Remarks on the ultimate legs prefemoral spinous formula of S. spinosissima plus a key to the species of the genus Scolopendra in the Philippines are provided.

 

References

  1. Altschul, S.F., Gish, W., Miller, W., Myers, E.W. & Lipman, D.J. (1990) Basic local alignment search tool. Journal of Molecular Biology, 215 (3), 403–410.

    https://doi.org/10.1016/S0022-2836(05)80360-2

    Attems, C. (1930) Myriopoda. 2. Scolopendromorpha. In: Das Tierreich. Vol. 54. W. De Gruyter, Berlin and Leipzig, pp. 1–308.

    Bonato, L., Chagas Junior, A., Edgecombe, G.D., Lewis, J.G.E., Minelli, A., Pereira, L.A., Shelley, R.M., Stoev, P. & Zapparoli, M. (2017) ChiloBase 2.0. A World Catalogue of Centipedes (Chilopoda). Available from: http://chilobase.biologia.unipd.it (accessed 10 February 2017)

    Bonato, L., Edgecombe, G., Lewis, J., Minelli, A., Pereira, L., Shelley, R. & Zapparoli, M. (2010) A common terminology for the external anatomy of centipedes (Chilopoda). ZooKeys, 69, 17–51.
    https://doi.org/10.3897/zookeys.69.737

    Brandt, J.F. (1840) Observations sur les espèces qui composent le genre Scolopendra suivies des caractères des espèces qui se trouvent dans le Muséum zoologique de l'Académie des Sciences de St. -Pétersbourg et de quelques coups d'oeil sur leur distribution géographique. Bulletin Scientifique, Académie Imperiale des Sciences de Saint Pétersbourg, 7 (11), 148–160.

    Chenna, R., Sugawara, H., Koike, T., Lopez, R., Gibson, T.J., Higgins, D.G. & Thompson, J.D. (2003) Multiple sequence alignment with the Clustal series of programs. Nucleic Acids Research, 31 (13), 3497–3500.
    https://doi.org/10.1093/nar/gkg500

    Demange, J.M. & Richard, J. (1969) Morphologie de l’appareil génital mâle des Scolopendromor-phes et son importance en systèmatique (Myriapodes Chilopodes). Bulletin du Muséum national d’Histoire naturelle, 40, 968–983.

    Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32 (5), 1792–1797.
    https://doi.org/10.1093/nar/gkh340

    Edgecombe, G.D. & Giribet, G. (2008) A New Zealand species of the trans-Tasman centipede order Craterostigmomorpha (Arthropoda: Chilopoda) corroborated by molecular evidence. Invertebrate Systematics, 22 (1), 1–15.
    https://doi.org/10.1071/IS07036

    Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294–299.

    Gervais, P. (1847) Myriapodes. In: Walckenaer, C.A. de & Gervais F.L.P., Histoire naturelle des Insectes Aptères. Tome 4. Librairie Encyclopédique de Roret, Paris, pp. 1–57 + 210–623.

    Guindon, S., Dufayard, J.F., Lefort, V., Anisimova, M., Hordijk, W. & Gascuel, O. (2010) New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic Biology, 59 (3), 307–21.
    https://doi.org/10.1093/sysbio/syq010

    Haase, E. (1887) Die Indisch-Australischen Myriopoden. 1. Chilopoden. Abhandlungen und Berichte des Königlichen zoologischen und anthropologisch-ethnographischen Museum zu Dresden, 4, 1–118.

    Huang, X. & Madan, A. (1999) CAP3: A DNA sequence assembly program. Genome Research, 9, 868–877.
    https://doi.org/10.1101/gr.9.9.868

    Iorio, E. (2003) Morphologie externe des appareils génitaux mâle et femelle de la famille Scolo-pendridae (Chilopoda, Scolopendromorpha). Bulletin de Phyllie, 16, 10–16.

    Jangi, B.S. & Dass, C.M.S. (1984) Scolopendridae of the Deccan. Journal of Scientific and Industrial Research, 43, 27–54.

    Joshi, J. & Karanth, K.P. (2011) Cretaceous-Tertiary diversification among select scolopendrid centipedes of South India. Molecular Phylogenetics and Evolution, 60, 287–294.
    https://doi.org/10.1016/j.ympev.2011.04.024
    Joshi, J. & Karanth, K.P. (2012) Coalescent methods in conjunction with niche modelling reveals cryptic diversity among centipedes in the Western Ghats of South India. PLoS ONE, 7 (8), e42225.

    https://doi.org/10.1371/journal.pone.0042225

    Kang, S., Liu, Y., Zeng, X., Deng, H., Luo, Y., Chen, k. & Chen, S. (2017) Taxonomy and identification of the genus Scolopendra in China using integrated methods of external morphology and molecular phylogenetics. Scientific Reports, 7, 16032.
    https://doi.org/10.1038/s41598-017-15242-7

    Kraepelin, K. (1903) Revision der Scolopendriden. Mitteilungen aus dem Naturhistorischen Museum in Hamburg, 20 (2), 1–276.

    Krishnamurthy P.K. and Francis, R.A. (2012) A critical review on the utility of DNA barcoding in biodiversity conservation. Biodiversity and Conservation, 21 (8), 1901–1919.
    https://doi.org/10.1007/s10531-012-0306-2

    Kronmüller, C. (2009) A new species of Scolopender from the Philippines (Chilopoda: Scolopendridae). Arthropoda, 17 (1), 48–51.

    Kronmüller, C. (2012) Review of the subspecies of Scolopendra subspinipes Leach, 1815 with the new description of the South Chinese member of the genus Scolopendra Linnaeus, 1758 named Scolopendra hainanum spec. nov. Spixiana, 35 (1) 19–27.

    Kronmüller, C. & Lewis, J.G.E. (2015) On the function of the ultimate legs of some Scolopendridae (Chilopoda, Scolopendromorpha). ZooKeys, 510, 269–278.
    https://doi.org/10.3897/zookeys.510.8674

    Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33, 1870–1874.

    Latreille, M. (1829) Les Myriapodes. Le régne animal distribué d'après son organisation, Nouvelle Édition, Revue et Augmentée. Tome IV. Crustacés, Arachnides et Partie des Insectes. Cuvier, PML, Paris, 4, 326–339.

    Lawrence, R.F. (1975) The Chilopoda of South West Africa. Cimbebasia, Series A, 4, 35–45.

    Leach, W.E. (1815) A tabular view of the external characters of four classes of animals, which Linné arranged under Insecta; with the distribution of the genera composing three of these classes into orders, &c. and descriptions of several new genera and species. Transactions of the Linnean Society of London, 11, 306–400.
    https://doi.org/10.1111/j.1096-36642.1813.tb00065.x

    Lewis, J.G.E. (1982) The scolopendrid centipedes of the Oxford University 1932 Sarawak Expedition. Journal of Natural History, 16, 389–397.
    https://doi.org/10.1080/00222938200770321

    Lewis, J.G.E. (2010) A key and annotated list of the Scolopendra species of the Old World with a reappraisal of Arthrorhabdus (Chilopoda: Scolopendromorpha: Scolopendridae). International Journal of Myriapodology, 3, 83–122.
    https://doi.org/10.1163/187525410X12578602960380

    Linnaeus, C. (1758) Systema naturae. Editio Decima. Salvius, Holmiae, 824 pp.

    Lucas, H. (1853) Essai sur les animaux articulés qui habitent l'île de Crete. Revue et Magasin de Zoologie Pure et Appliquée, 5 (2), 61–76 + 418–424 + 514–531.

    Mengual, X. (2008) Molecular phylogeny and evolution of predatory Syrphidae (Insecta: Diptera). Publicacions Universitat d’Alacant, Espanya, 254 pp.

    Meinert, F. (1886) Myriapoda Musei Cantabrigiensis. Part I. Chilopoda. Proceedings of the American Philosophical Society, 23, 161–233.
    https://doi.org/10.5962/bhl.part.26797

    Murienne, J., Edgecombe, G.D. & Giribet, G. (2010) Including secondary structure, fossils and molecular dating in the centipede tree of life. Molecular Phylogenetics and Evolution, 57, 301–313.
    https://doi.org/10.1016/j.ympev.2010.06.022

    Murienne, J., Edgecombe, G.D. & Giribet, G. (2011) Comparative phylogeography of the centipedes Cryptops pictus and C. niuensis (Chilopoda) in New Caledonia, Fiji and Vanuatu. Organisms Diversity and Evolution, 11 (1), 61–74.
    https://doi.org/10.1007/s13127-011-0041-7

    Nei, M. & Kumar, S. (2000) Molecular Evolution and Phylogenetics. Oxford University Press, New York, 352 pp.

    Newport, G. (1844) A list of the species of Myriapoda order Chilopoda contained in the cabinets of the British Museum with synoptic descriptions of forty-seven new species. Annals and Magazine of Natural History, 13, 94–101.
    https://doi.org/10.1080/03745484409442576

    Oeyen, J.P., Funke, S., Böhme, W. & Wesener, T. (2014) The evolutionary history of the rediscovered Austrian population of the giant centipede Scolopendra cingulata Latreille 1829 (Chilopoda, Scolopendromorpha). PLoS ONE, 9 (9), e108650.
    https://doi.org/10.1371/journal.pone.0108650

    Pocock, R.I. (1891a) On the Myriopoda of Burma. Part 2. Report upon the Chilopoda collected by Sig. L. Fea and Mr. E. W. Oates. Annali del Museo Civico di Storia Naturale di Genova, 30, 401–432.

    Pocock, R.I. (1891b) Notes on the synonymy of some species of Scolopendridae with descriptions of new genera and species of the group.Annals and Magazine of Natural History, (6)7, 51–58 + 221–31.

    Pontarotti, P. (2008). Evolutionary Biology from Concept to Application. Springer-Verlag, Berlin, 220 pp.
    https://doi.org/10.1007/978-3-540-78993-2

    Porat, C. (1876) Om några exotiska Myriopoder. Bihang till Kongliga Svenska Vetenskaps-Akademien Handligar, 4, 1–48.

    Ribaut, H. (1923) Chilopodes de la Nouvelle-Calédonie et des Îles Loyalty. In: Sarasin, F. & Roux, J., Recherches Scientifiques en Nouvelle-Calédonie et aux Îles Loyalty. C.W. Kreidel's Verlag, Berlin, Weisbaden, 79 pp.

    Saitou, N. & Nei, M. (1987) The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4, 406–425.

    Say, T. (1821) Description of the Myriapodæ of the United States. Journal of the Academy of Natural Sciences of Philadelphia, 2 (1), 102–114.

    Siriwut, W., Edgecombe, G.D., Sutcharit, C. & Panha, S. (2015) The centipede genus Scolopendra in mainland Southeast Asia: molecular phylogenetics, geometric morphometrics and external morphology as tools for species delimitation. PLoS ONE, 10 (8), e0135355.
    https://doi.org/10.1371/journal.pone.0135355

    Siriwut, W., Edgecombe, G.D., Sutcharit, C., Tongkerd, P. & Panha, S. (2016) A taxonomic review of the centipede genus Scolopendra Linnaeus, 1758 (Scolopendromorpha, Scolopendridae) in mainland Southeast Asia, with description of a new species from Laos. ZooKeys, 590, 1–124.
    https://doi.org/10.3897/zookeys.590.7950

    Takakuwa Y. (1942) Die Myriopoden von Formosa, Philippinien, u. s. w. Transactions of the Natural History Society of Formosa, 32, 359–367.

    Tamura, K. & Nei, M. (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution, 10, 512–526.

    Tyagi, K,. Kumar, V., Singha, D., Chandra, k., Laskar, B.A., Kundu, S., Chakraborty, R. & Chatterjee, S. (2017) DNA Barcoding studies on Thrips in India: Cryptic species and species complexes. Scientific Reports, 7, 4898.
    https://doi.org/10.1038/s41598-017-05112-7

    Vahtera, V., Edgecombe, G.D. & Giribet, G. (2012) Evolution of blindness in scolopendromorph centipedes (Chilopoda: Scolopendromorpha): insight from an expanded sampling of molecular data. Cladistics, 28, 4–20.

    Vahtera, V., Edgecombe, G.D. & Giribet, G. (2013) Phylogenetics of scolopendromorph centipedes: can denser taxon sampling improve an artificial classification? Invertebrate Systematics, 27 (5), 578–602.
    https://doi.org/10.1071/IS13035

    Wang, Y.-H.M. (1951) The Myriapoda of the Philippine Islands. Serica I. Dissertation PhD, University of Utah, ‎Salt Lake City, Utah‎, 80pp.

    Wang, Y.-H.M. (1962) Serica Im: The Chilopoda of the Philippine Islands (A Revision of the Myriapoda of the Philippine Islands Part 2). Quarterly Journal of the Taiwan Museum, 15, 79–106.