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Issue: Vol. 4496 No. 1: 5 Oct. 2018
Type: Article
Published: 2018-10-04
DOI: 10.11646/zootaxa.4496.1.5
Page range: 43–64
Abstract views: 92
PDF downloaded: 1

DNA barcoding of the Italian anecic Octodrilus species in rural (vineyard) and forested areas with description of Octodrilus zicsiniello sp. nov. (Clitellata, Megadrili)

Department of Zoology, Eszterházy Károly University, Eger, Hungary.
Department of Zoology, Hungarian Natural History Museum, Budapest, Hungary.
Universidad Complutense de Madrid, Departamento de Zoología y Antropología Física, Madrid, Spain.
Universidad Complutense de Madrid, Departamento de Zoología y Antropología Física, Madrid, Spain.
Università degli Studi di Padova, Dipartimento di Biologia, Padova, Italy.
Museo Friulano di Storia Naturale, Udine, Italy.
Università degli Studi di Padova, Dipartimento di Biologia, Padova, Italy.
Università degli Studi di Padova, Dipartimento di Biologia, Padova, Italy.
Università degli Studi di Padova, Dipartimento di Biologia, Padova, Italy.
ESAPOLIS, Padova, Italy.
Università degli Studi di Padova, Dipartimento di Biologia, Padova, Italy.
Annelida Oligochaeta COI Alps new species Octodrilus anecic vineyards Collio Prosecco

Abstract

DNA barcoding of 172 anecic Octodrilus specimens collected in NE Italy and bordering Croatia has been carried out. The Bayesian phylogenetic tree showed high support for almost all currently recognized species, however, some unexpected results also appeared. The clade representing Oc. pseudocomplanatus contains a highly advanced subclade, which morphologically resembles Oc. slovenicus. The highly supported Oc. tergestinus clade consists of four unresolved divergent lineages of which the first corresponds to Oc. istrianus and the second resembles Oc. mimus morphologically; the third and fourth clades show typical tergestinus characters. The widely distributed Oc. complanatus consists of three highly divergent subclades which are sister to a new species, Oc. zicsiniello sp. nov., hereunder described.

 

References

  1. Akaike, H. (1973) Maximum likelihood identification of Gaussian autoregressive moving average models. Biometrika, 60, 255–265.
    https://doi.org/10.1093/biomet/60.2.255

    Chang, C.-H. & James, S.W. (2011) A critique of earthworm molecular phylogenetics. Pedobiologia, 54, 3–9. [S3–S9]
    https://doi.org/10.1016/j.pedobi.2011.07.015

    Cognetti, L. (1901) Res Italicae I. Octolasium hemiandrum nov. sp. ed altri Lumbricidi raccolti del Dott. E. Festa nei dintorni della Spezia. Bollettino dei Musei di Zoologia ed Anatomia comparata della R. Universitá di Torino, 16 (383), 3.

    Csuzdi, Cs. (2012) Earthworm species, a searchable database. Opuscula Zoologica Budapest, 43 (1), 97–99.

    Csuzdi, Cs., Pop, V.V. & Pop, A.A. (2011) The earthworm fauna of the Carpathian Basin with new records and description of three new species (Oligochaeta: Lumbricidae). Zoologischer Anzeiger, 250, 2–18.
    https://doi.org/10.1016/j.jcz.2010.10.001

    Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature methods, 9 (8), 772–772.
    https://doi.org/10.1038/nmeth.2109

    Decaëns, T., Porco, D., James, S.W., Brown, G.G., Chassany, V., Dubs, F., Dupont, L., Lapied, E., Rougerie, R., Rossi J.-P. & Roy, V. (2016) DNA barcoding reveals diversity patterns of earthworm communities in remote tropical forests of French Guiana. Soil Biology and Biochemistry, 92, 171–183.
    https://doi.org/10.1016/j.soilbio.2015.10.009

    Dugès, A. (1828) Recherche sur la circulation, la respiration, et la reproduction des Annélides sétigères abranches. Annales des Sciences Naturelles Paris, 15, 284–336.

    Fusaro, S., Gavinelli, F., Lazzarini, F. & Paoletti, M.G. (2018) Soil Biological Quality Index based on earthworms (QBS-e). A new way to use earthworms as bioindicators in agroecosystems. Ecological Indicators. [accepted]

    Gavinelli, F., Barcaro, T., Csuzdi, Cs., Blakemore R., Fernandez Marchan, D., De Sosa, I., Dorigo, L., Lazzarini, F., Nicolussi, G., Leandro Dreon, A., Toniello, V., Pamio, A., Squartini, A., Concheri, G., Moretto, E. & Paoletti, M.G. (2017) Importance of the large deep-burrowing (anecic) earthworms in forested and cultivated areas (especially vineyards) of the North-Eastern Italy. Applied Soil Ecology. [in press]
    https://doi.org/10.1016/j.apsoil.2017.07.012

    Hackenberger Kutuzović, D. & Hackenberger Kutuzović, B. (2013) Checklist of the earthworm fauna of Croatia (Oligochaeta: Lumbricidae). Zootaxa, 3710 (1), 1–30.
    https://doi.org/10.11646/zootaxa.3710.1.1

    Karaman, S. (1972) Beitrag zur Kenntnis der Oligochaetenfauna Judoslawiens. Biološki vestnik, 20, 95–105.

    Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution, 30 (4), 772–780.
    https://doi.org/10.1093/molbev/mst010

    Kimura, M. (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16, 111–120.
    https://doi.org/10.1007/BF01731581

    Michaelsen, W. (1900) Oligochaeta. In: Das Tierreich. Vol. 10. Vermes, Berlin, pp. 1–575.

    Michaelsen, W. (1910a) Zur Kenntnis der Lumbriciden und ihrer Verbreitung. Annuaire du Musée Zoologique de l'Académie Impériale des Sciences de St.-Pétersbourg, 15, 1–74.

    Michaelsen, V. (1910b) Oligochäten von verschiedenen Gebieten. Mitteilungen aus dem Naturhistorischen Museum in Hamburg, 27, 47–169.

    Mršić, N. (1991) Monograph on earthworms (Lumbricidae) of the Balkans I, II. Slovenska Akademija Znanosti in Umetnosti, Razred za Naravoslovne Vede, Opera 31, 1–757. [Ljubljana]

    Nicolussi, G. (2016) Grandi lombrichi profondi scavatori (Octodrilus mimus ed Octodrilus tergestinus), endemici dell'Italia nord orientale, difendono il loro territorio con ferormoni di allarme dalla specie invasiva Lumbricus terrestris? MSc thesis, Università degli Studi di Padova, Padova, 61 pp.

    Novo, M., Almodovar, A., Fernandez, R., Giribet, G. & Diaz Cosin, D.J. (2011) Understanding the biogeography of a group of earthworms in the Mediterranean basin-The phylogenetic puzzle of Hormogastridae Clitellata: Oligochaeta). Molecular Phylogenetics and Evolution, 61, 125–135.
    https://doi.org/10.1016/j.ympev.2011.05.018

    Novo, M., Fernández, R., Fernández Marchán, D., Trigo, D., Díaz Cosín, D.J. & Giribet, G. (2015) Unearthing the historical biogeography of Mediterranean earthworms (Annelida: Hormogastridae). Journal of Biogeography, 42 (4), 751–762.
    https://doi.org/10.1111/jbi.12447

    Omodeo, P. (1954) Alcuni delle lombrici Alpi Venete e della costa orientale dell'Adriatico. Atti del Museo Civico di Storia Naturale di Trieste, 19, 120–135.

    Omodeo, P. (1956) Contributo alla revisione dei Lumbricidae. Archivio Zoologico Italiano, 41, 129–212.

    Omodeo, P. (1962) Oligochètes des Alpes. I. Memorie del Museo Civico di Storia Naturale di Verona, 10, 71–96.

    Paoletti, M.G. (1988) Soil invertebrates in cultivated and uncultivated soils in North-East Italy. Redia, 71, 501–563.

    Paoletti, M.G., Sommaggio, D. & Fusaro, S. (2013) Proposta di Indice di Qualità Biologica del Suolo (QBS-e) basato sui Lombrichi e applicato agli Agroecosistemi. Biologia Ambientale, 27 (2), 25–43.

    Paoletti, M.G., Blakemore, R.J., Csuzdi, Cs., Dorigo, L., Dreon, A.L., Gavinelli, F., Lazzarini, F., Manno, N., Moretto, E., Porco, E., Ruzzier, E., Toniello, V., Squartini, A., Concheri, G., Zanardo, M. & Alba-Tercedor, J. (2016) Barcoding Eophila crodabepis sp. nov. (Annelida, Oligochaeta, Lumbricidae), a Large Stripy Earthworm from Alpine Foothills of Northeastern Italy Similar to Eophila tellinii (Rosa, 1888). PLoS ONE, 11 (3), e0151799.
    https://doi.org/10.1371/journal.pone.0151799

    Pavlíček, T. & Csuzdi, Cs. (2016) Chapter 23: Clitellata: Oligochaeta: Earthworms. In: Sparrow, D.J. & Eddie, J. (Eds.), An Introduction to the Wildlife of Cyprus. Terra Cypria, Limassol, pp. 587–599.

    Pérez-Losada, M., Ricoy, M., Domínguez, J. & Marshall, J. (2009) Phylogenetic assessment of the earthworm Aporrectodea caliginosa species complex (Oligochaeta, Lumbricidae) based on mitochondrial and nuclear DNA sequences. Molecular Phylogenetics and Evolution, 52, 293–302.
    https://doi.org/10.1016/j.ympev.2009.04.003

    Pérez-Losada, M., Breinholt, J.W., Aira, M., & Domínguez, J. (2015) An Updated Multilocus Phylogeny of the Lumbricidae (Annelida: Clitellata: Oligochaeta) Earthworms. Journal of Phylogenetics & Evolutionary Biology, 3, 140.
    https://doi.org/10.4172/2329-9002-3-140

    Pop, V.V. (1994) On the speciation in the Genus Octodrilus Omodeo, 1956 (Oligochaeta, Lumbricidae). Mitteilungen aus dem Hamburgischen zoologischen Museum und Institut, 89 (2), 37–46.

    Pop, V.V. & Pop, A.A. (2004) A comprehensive study of the Taxonomy and Ecology of the Lumbricid Earthworm Genus Octodrilus from the Carpathians. In: Edwards, C.E. (Ed.), Earthworm Ecology. 2nd Edition. Lucie Press, Boca Raton, pp. 115–141.
    https://doi.org/10.1201/9781420039719.ch7

    Ronquist, F. & Huelsenbeck, J.P. (2003) MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574.
    https://doi.org/10.1093/bioinformatics/btg180

    Rosa, D. (1889) Descrizione dell' Allololbophora mima n. sp. Bollettino dei Musei di Zoologia ed Anatomia comparata della R. Universitá di Torino, 4 (60), 1–2.

    Rota, E. & de Jong, Y. (2015) Fauna Europaea: Annelida - Terrestrial Oligochaeta (Enchytraeidae and Megadrili), Aphanoneura and Polychaeta. Biodiversity Data Journal, 3, e5737.
    https://doi.org/10.3897/BDJ.3.e5737

    Schwarz, G.E. (1978) Estimating the dimension of a model. Annals of Statistics, 6, 461–464.
    https://doi.org/10.1214/aos/1176344136

    Schmelz, R.M. & Timm, T. (2007) Advocating paraphyletic taxa in systematics of Clitellata. Acta Hydrobiologica Sinica, 31 (Supplement), 99–108.

    Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729.
    https://doi.org/10.1093/molbev/mst197

    Zicsi, A. (1981) Weitere Angaben zur Lumbricidenfauna Italiens (Oligochaeta: Lumbricidae). Opuscula Zoologica, Budapest, 17–18, 157–180.

    Zicsi, A. (1982) Verzeichnis der bis 1971 beschriebenen und revidierten Taxa der Familie Lumbricidae (Oligochaeta). Acta Zoologica Hungarica, 28, 421–454.

    Zicsi, A. (1986) Über die taxonomische Probleme der Gattung Octodrilus Omodeo, 1956 und Octodriloides gen. n. (Oligochaeta: Lumbricidae). Opuscula Zoologica Budapest, 21, 137–139.