Abstract
The earthworm genus Pheretima Kinberg, 1867 sensu stricto is recorded in Vietnam for the first time with a new species, P. vungtauensis sp. nov. The new species is characterized by three pairs of spermathecal pores in intersegments 6/7/8/9, spermathecal ducts with dense micronephridia, no genital markings in spermathecal and male regions, male pores located inside copulatory pouches, intestinal origin at xv, caeca simple from xxvii, and holandry. DNA-COI barcode sequences of the holotype are most similar to those of Metaphire houlleti (Perrier, 1872) from Thailand and not to other species of Pheretima.
References
Aspe, N.M. & James, S.W. (2014) New species of Pheretima (Oligochaeta: Megascolecidae) from the Mt. Malindang Range, Mindanao Island, Philippines. Zootaxa, 3881 (1), 401–439.
https://doi.org/10.11646/zootaxa.3881.5.1Aspe, N.M. & James, S.W. (2016) New species of Pheretima, Amynthas, Polypheretima, and Pithemera (Clitellata: Megascolecidae) from Mindanao and associated islands, Philippines. Zoological Studies, 55 (8), 1–33.
https://doi.org/10.6620/ZS.2016.55–08.Aspe, N.M., Kajihara, H. & James, S.W. (2016) A molecular phylogenetic study of pheretimoid species (Megascolecidae) in Mindanao and associated islands, Philippines. European Journal of Soil Biology, 73, 119–125.
https://doi.org/10.1016/j.ejsobi.2016.02.006Blakemore, R.J. (2007) Updated checklist of Pheretimoids (Oligochaeta: Megascolecidae: Pheretima auct.) taxa. Available from: http://www.annelida.net/earthworm/Pheretimoids.pdf (accessed 9 December 2017)
Blakemore, R.J. (2016) Darwin’s earthworms (Annelida, Oligochaeta, Megadrilacea) with review of cosmopolitan Metaphire peguana–species group from Philippines. Opuscula Zoologica, Budapest, 47 (1), 9–30.
https://doi.org/10.18348/opzool.2016.1.9Chang, C.-H. & James, S. (2011) A critique of earthworm molecular phylogenetics. Pedobiologia, 54S, 3–9. [S3–S9]
https://doi.org/10.1016/j.pedobi.2011.07.015Easton, E.G. (1979) A revision of the 'acaecate' earthworms of the Pheretima group (Megascolecidae: Oligochaeta): Archipheretima, Metapheretima, Planapheretima, Pleionogaster, and Polypheretima. Bulletin of the British Museum of Natural History, London, Zoology, 35, 1–126.
https://doi.org/10.5962/bhl.part.20451Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular marine biology and biotechnology, 3 (5), 294–299.
Górny, M. & Grum, L. (1993) Methods in Soil Zoology. Elsevier Science, Dordrecht, 492 pp.
Hong, Y. & James, S.W. (2010) Six new earthworms of the genus Pheretima (Oligochaeta: Megascolecidae) from Balbalan-Balbalasang, Kalinga Province, the Philippines. Zoological Studies, 49 (4), 523–533.
Hong, Y. & James, S.W. (2011) New earthworm species of the genus Pheretima (Clitellata: Megascolecidae) from Mountain Province, Philippines. Journal of Natural History, 45, 1769–1788.
https://doi.org/10.1080/00222933.2011.560726Huelsenbeck, J.P. & Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics, 17, 754–755.
https://doi.org/10.1093/bioinformatics/17.8.754Jeratthitikul, E., Bantaowong, U. & Panha, S. (2017) DNA barcoding of the Thai species of terrestrial earthworms in the genera Amynthas and Metaphire (Haplotaxida: Megascolecidae). European Journal of Soil Biology, 81, 39–47.
https://doi.org/10.1016/j.ejsobi.2017.06.004Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA 7: Molecular Evolution Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33, 1870–1874.
https://doi.org/10.1093/molbev/msw054Nguyen, T.T., Nguyen, A.D., Tran, T.T.B. & Blakemore, R. (2016) A comprehensive checklist of earthworm species and subspecies from Vietnam (Annelida: Clitellata: Oligochaeta: Almidae, Eudrilidae, Glossoscolecidae, Lumbricidae, Megascolecidae, Moniligastridae, Ocnerodrilidae, Octochaetidae). Zootaxa, 4140 (1), 1–92.
https://doi.org/10.11646/zootaxa.4140.1.1Perrier, E. (1872) Recherches pour servir à l’histoire des Lombriciens terrestres. Nouvelles Archives du Muséum d’Histoire Naturelle de Paris, 8, 5–198.
Sims, R.W. & Easton, E.G. (1972) A numerical revision of the earthworm genus Pheretima auct. (Megascolecidae: Oligochaeta) with the recognition of new genera and an appendix on the earthworms collected by the Royal Society North Borneo Expedition. Biological Journal of the Linnean Society, 4, 169–268.
https://doi.org/10.1111/j.1095-8312.1972.tb00694.x