Skip to main content Skip to main navigation menu Skip to site footer
Type: Article
Published: 2018-10-18
Page range: 553–573
Abstract views: 163
PDF downloaded: 4

A new species of Crocodile Newt Tylototriton (Caudata: Salamandridae) from Shan State, Myanmar (Burma)

Herpetology Laboratory, Department of Biology, La Sierra University, 4500 Riverwalk Parkway, Riverside, California 92515, USA
Department of Ecology and Evolutionary Biology and Biodiversity Institute, University of Kansas, Dyche Hall, 1345 Jayhawk Blvd, Lawrence, Kansas 66045-7561, USA.
School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia.
Myanmar Environment Sustainable Conservation, Yangon, Myanmar.
Department of Biology, California State University, Northridge, 18111 Nordhoff Street, Northrdge, California 91330-8303, USA.
Herpetology Laboratory, Department of Biology, La Sierra University, 4500 Riverwalk Parkway, Riverside, California 92515, USA.
Department of Biology, Villanova University, 800 Lancaster Avenue, Villanova, Pennsylvania 19085, USA.
Fauna and Flora International, No(35), 3rd Floor, Shan Gone Condo, Myay Ni Gone Market Street, Sanchaung Township, Yangon, Myanmar.
Reptilia Integrative taxonomy Tylototriton Shan State Ywangan new species Myanmar conservation pet trade

Abstract

A phylogenetic taxonomic analysis of a monophyletic subgroup of the salamandrid genus Tylototriton revealed that a newly discovered population from Ngar Su Village, 1 km south of Ywangan, Shan State, Myanmar is a new species and most closely related to T. shanorum from approximately 80 km to the west in the vicinity of Taunggyi, Shan State. Tylototriton ngarsuensis sp. nov. differs from other closely related species of Tylototriton on basis of varying combinations of characteristics associated with it shorter head, larger size, rib nodule morphology, and overall drab, very dark, coloration, along with its genetic differentiation. Tylototriton ngarsuensis sp. nov. also appears to breed later in the year than most other species. Unfortunately, this species like many other Asian newts, is being harvested for the pet and medicinal trade and given its restricted distribution, this could pose a serious threat to its long-term survival.

 

References

  1. Abdala, C.S., Baldo, D., Juárez, R.A. & Espinoza, R.E. (2016) The first parthenogenetic pleurodont iguanian: A new all-female Liolaemus (Squamata: Liolaemidae) from western Argentina. Copeia, 104, 487–497.
    https://doi.org/10.1643/ch-15-381

    Acinas, S.G., Klepac-Ceraj, V., Hunt, D.E., Pharino, C., Ceraj, I., Distel, D.L. & Polz, M.F. (2004) Fine-scale phylogenetic architecture of a complex bacterial community. Nature, 430, 551–554.
    https://doi.org/10.1038/nature02649

    Barraclough, T.G., Birky, C.W. Jr. & Burt, A. (2003) Diversification in sexual and asexual organisms. Evolution, 57, 2166–2172.
    https://doi.org/10.1554/02-339

    Bernardes, M.B., Rauhaus, A., Michel, C., Pham, C.T., Nguyen, T.Q., Le, M.D., Pasmans, F., Bonkowski, M. & Ziegler, T. (2017) Larval development and breeding ecology of Ziegler’s Crocodile Newt, Tylototriton ziegleri Nishikawa, Matsui and Nguyen, 2013 (Caudata: Salamandridae), compared to other Tylototriton representatives. Amphibian & Reptile Conservation, 11, 72–87.

    Bernardes, M., Rödderb, D., Nguyen, T.T., Pham, C.T., Nguyen, T.Q. & Ziegler, T. (2013) Habitat characterization and potential distribution of Tylototriton vietnamensis in northern Vietnam. Journal of Natural History, 47, 1161–1175.

    Coyne, J.A. & Orr, H.A. (1998) The evolutionary genetics of speciation. Philosophical Transactions of the Royal Society of London B, 353, 287–305.
    https://doi.org/0.1098/rstb.1998.0210

    Dasgupta, R. (1996) Feeding ecology of the adult Himalayan Salamander Tylototriton verrucosus Anderson, 1871. Herpetozoa, 9, 19–29.

    de Queiroz, K. (2007) Species concepts and species delimitation. Systemtic Biology, 56, 879–886.
    https://doi.org/10.1080/10635150701701083

    Drummond, A.J., Suchard, M.A., Xie, D. & Rambaut, A. (2012) Bayesian Phylogenetics with BEAUti and BEAST 1.7. Molecular Biology and Evolution, 29, 1969–1973.
    https://doi.org/10.1093/molbev

    Dubois, A. & Raffaëlli, J. (2009) A new ergotaxonomy of the family Salamandridae Goldfuss, 1820 (Amphibia, Urodela). Alytes, 26, 1–85.

    Fei, L., Ye, C.Y., Jiang, J.P. (2012) Colored atlas of Chinese amphibians and their distributions. Sichuan Publishing House of Science and Technology, Chengdu, 1620 pp.

    Fontaneto, D., Herniou, E.A., Boschetti, C., Caprioli, M., Melone, G., Ricci, C. & Barraclough, T.G. (2007) Independently evolving species in asexual bdelloid rotifers. PLoS Biology, 5, e87.
    https://doi.org/10.1371/journal.pbio.0050087

    Frost, D.R. (2018) Amphibian Species of the World: an Online Reference. Version 6.0. American Museum of Natural History, New York, USA. Electronic Database. Available from: http://research.amnh.org/herpetology/amphibia/index.html (accessed 2 October 2018)

    Fujisawa, T. & Barraclough, T.G. (2013) Delimiting species using single-locus data and the Generalized Mixed Yule Coalescent approach: a revised method and evaluation on simulated data sets. Systematic Biology, 62 707–724.
    https://doi.org/10.1093/sysbio/syt033

    Gong, D.J. & Mu, M. (2008) Behavioural observations and descriptions of the endangered Knobby Newt Tylototriton wenxianensis and their application in conservation. Asiatic Herpetological Research, 1, 31–38.

    Grismer, L.L., Wood Junior, P.L., Thura, M.K., Zin, T., Quah, E.S.H., Murdoch, M.L., Grismer, M.S., Lin, A., Kyaw, H. & Ngwe, L. (2017a) Twelve new species of Cyrtodactylus Gray (Squamata: Gekkonidae) from isolated limestone habitats in east-central and southern Myanmar demonstrate high localized diversity and unprecedented microendemism. Zoological Journal of the Linnean Society, 182, 862–959.
    https://doi.org/10.1093/zoolinnean/zlx057

    Grismer, L.L., Wood Junior, P.L., Thura, M.K., Zin, T., Quah, E.S.H., Murdoch, M.L., Grismer, M.S., Lin, A., Kyaw, H. & Ngwe, L. (2017b) Phylogenetic taxonomy of Hemiphyllodactylus Bleeker, 1860 (Squamata: Gekkonidae) with descriptions of three new species from Myanmar. Journal of Natural History, 52 (13–16), 881–915.
    https://doi.org/10.1080/00222933.2017.1367045

    Grismer, L.L., Wood Junior, P.L., Thura, M.K., Zin, T., Quah, E.S.H., Murdoch, M.L., Grismer, M.S., Herr, M.W., Lin, A. & Kyaw, H. (2018a) Three more new species of Cyrtodactylus (Squamata: Gekkonidae) from the Salween Basin of eastern Myanmar underscores the urgent need for the conservation of karst habitats. Journal of Natural History, 52, 1243–1294.

    https://doi.org/10.1080/00222933.2018.1449911

    Grismer, L.L., Wood Junior, P.L., Myint, M.K., Quah, E.S.H., Grismer, M.S., Murdoch, M.L., Espinoza, R.E. & Aung Lin. (2018b) A new Cyrtodactylus Gray (Squamata, Gekkonidae) from the Shan Hills and the biogeography of Bent-toed Geckos from eastern Myanmar. Zootaxa, 4446 (4), 477–500.
    https://doi.org/10.11646/zootaxa.4446.4.4

    Grismer, L.L., Wood Junior, P.L., Thura, M.K., Zin, T., Win, N.M., Grismer, M.S., trueblood, L.A. & Quah, E.S.H. (2018c) A re-description of Cyrtodactylus chrysopylos Bauer (Squamata: Gekkonidae) with comments on the adaptive significance of bright coloration in hatchlings and descriptions of two new species from eastern Myanmar (Burma). Zootaxa, 4446, 477-–500.

    Grosse, W.-R. (2013) Kommentierte Liste zur Bestimmung der Entwicklungsstadien von Schwanzlurchen Amphibia: Urodela. In: Schultschik, G., Grosse, W.-R., Mannheim (Eds.), Gefährdete Molch- und Salamanderarten-Richtlinien für Erhaltungszuchten. Mertensiella. Chimaira, Frankfurt am Main, 189 pp.

    Hoang, D.T., Chernomor, O., von Haeseler, A., Minh, B.Q. & Le, S.V. (2017) UFBoot2: Improving the Ultrafast Bootstrap Approximation. Moloecular Biology and Evolution, 35, 518–522.
    https://doi.org/10.1093/molbev/msx281

    Hou, M., Li, P. & Lü, S.-Q. (2012) Morphological research development of genus Tylototriton and primary confirmation of the status of four cryptic populations. Journal of Huangshan University, 14, 61–65.

    Huelsenbeck, J.P., Ronquist, F., Nielsen, R. & Bollback, J.P. (2001) Bayesian Inference of phylogeny and its impact on evolutionary biology. Science, 294, 2310–2314.
    https://doi.org/10.1126/science.1065889

    Jun, L., Liu, A.Z., Xiang, L., Liu, X.H. & Kai, J. (2012) The breeding ecology of Red Knobby Newts, Tylototriton shanjing. Chinese Journal of Zoology, 47, 8–15.

    Katoh, M. & Kuma, M. (2002) MAFTT: a novel method for rapid sequence alignment based on fast Fourier transform. Nucleic Acids Research, 30, 3059–3066.
    https://doi.org/10.1093/nar/gkf436

    Kearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., Buxton, S., Cooper, A., Markowitz, S., Duran, C., Thierer, T., Ashton, B., Meintjes, P. & Drummond, A.J. (2012) Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics, 28, 1647–1649.
    https://doi.org/10.1093/bioinformatics/bts199

    Khatiwada, J.R., Wang, B., Ghimire, S., Vasudevan, K., Paudel, S. & Jiang, J.P. (2015) A new species of the genus Tylototriton (Amphibia: Urodela: Salamandridae) from eastern Himalaya. Asian Herpetological Research, 6, 245–256.

    Knowles, L.L. & Carstens, B.C. (2007) Delimiting species without monophyletic gene trees. Systematic Biology, 56, 887–895.
    https://doi.org/10.1080/10635150701701091

    Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: Molecular evolutionary genetics analaysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33, 1870-–1874.
    https://doi.org/10.1093/molbev/msw054

    Kuzmin, S.L., Dasgupta, R. & Smirina, E.M. (1994) Ecology of the Himalayan Newt (Tylototriton verrucosus) in Darjeeling Himalayas, India. Russian Journal of Herpetology, 1, 69–76.

    Le, D.T., Nguyen, T.T., Nishikawa, K., Nguyen, S.L.H., Pham, A.V., Matsui, M., Bernardes, M. & Nguyen, T.Q. (2015) A new species of Tylototriton Anderson, 1871 (Amphibia: Salamandridae) from northern Indochina. Current Herpetology, 34, 38–50.
    https://doi.org/10.5358/hsj.34.38

    Leaché, A.D., Koo, M.S., Spencer, C.L., Papenfuss, T.J., Fisher, R.N. & McGuire, J.A. (2009) Quantifying ecological, morphological, and genetic variation to delimit species in the Coast Horned Lizard species complex (Phrynosoma). Proceedings of the National Academy of Sciences, 106, 12418–12423.
    https://doi.org/10.1073/pnas.0906380106

    Lleonart, J., Salat, J. & Torres, G.J. (2000) Removing allometric effects of body size in morphological analysis. Journal of Theoretical Biology, 205, 85–93.
    https://doi.org/10.1006/jtbi.2000.2043

    Macey, J.J., Larson, A., Ananjeva, N.B., Fang, Z. & Papenfuss, T.J. (1997) Two novel gene orders and the role of light-strand replication in rearrangement of the vertebrate mitochondrial genome. Molecular Biology and Evolution, 14, 91–104.

    Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Proceedings of the Gateway Computing Environments Workshop (GCE), New Orleans, LA, 14 November 2010, pp. 1–8.
    https://doi.org/10.1109/GCE.2010.5676129

    Maddison, W.P. & Maddison, D.R. (2015) Mesquite: a modular system for evolutionary analysis. Version 3.04. Available from: http://mesquiteproject.org (accessed 2 October 2018)
    https://doi.org/10.1093/sysbio/42.2.218

    Nguyen, L.-T., Schmidt, H.A., von Haeseler, A. & Minh, B.Q. (2015) IQ-TREE: A fast and effective stochastic algorithm for estimating maximum likelihood phylogenies. Molecular Biology and Evolution, 32, 268–274.
    https://doi.org/10.1093/molbev/msu300

    Nishsikawa, K., Jian, J.-P. & Masafumi, M. (2011) Two new species of Pachytriton from Anhui and Guangxi, China (Amphibia: Urodela: Salamandridae). Current Herpetology, 30, 15–31.
    https://doi.org/10.3105/018.030.0102

    Nishikawa, K., Khonsue, W., Pomchote, P. & Matsui, M. (2013a) Two new species of Tylototriton from Thailand (Amphibia: Urodela: Salamandridae). Zootaxa, 3737 (3), 261–279.
    https://doi.org/10.11646/zootaxa.3737.3.5

    Nishikawa, K., Matsui, M. & Nguyen, T.T. (2013b) A new species of Tylototriton from northern Vietnam (Amphibia: Urodela: Salamandridae). Current Herpetology, 32, 34–49.
    https://doi.org/10.5358/hsj.32.34

    Nishikawa, K., Matsui, M. & Rao, D. (2014) A new species of Tylototriton (Amphibia: Urodela: Salamandridae) from central Myanmar. Natural History Bulletin of the Siam Society, 60, 9–22.

    Nishikawa, K., Rao, D.-Q., Matsui, M. & Eto, K. (2015) Taxonomic relationship between Tylototriton daweishanensis Zhao, Rao, Liu, Li and Yuan, 2012 and T. yangi Hou, Li and Lu, 2012 (Amphibia: Urodela: Salamandridae). Current Herpetology, 34, 67–74.
    https://doi.org/10.5358/hsj.34.67

    Phimmachak, S., Aowphol, A. & Stuart, B.L. (2015) Morphological and molecular variation in Tylototriton (Caudata: Salamandridae) in Laos, with description of a new species. Zootaxa, 4006 (2), 285–310.
    https://doi.org/10.11646/zootaxa.4006.2.3

    Pons, J., Barraclough, T.G., Gomez-Zurita, J., Cardoso, A., Duran, D.P, Hazell, S., Kamoun, S., Sumlin, W.D. & Vogler, A.P. (2006) Sequence-based species delimitation for the DNA taxonomy of undescribed insects. Systematic Biology, 55, 595–609. https://doi.org/10.1080/10635150600852011

    Qian, L., Sun, X., Li, J. Guo, W., Pan, T., Kang, X., Wang, H., Jian, J., Wu, J. & Zhang, B. (2017) A new species of the genus Tylototriton (Amphibia: Urodela: Salamandridae) from the Southern Dabie Mountains in Anhui Province. Asian Herpetological Research, 8, 151–164.

    R Core Team (2015) R: A language and environment for statistical computing. R Foundation for Statistical Computing. Vienna. Available from http://www.R-project.org (accessed 28 December 2016)

    Rambaut, A. & Drummond, A.J. (2013) TreeAnnotator. Version. 1.8.0. MCMC Output Analysis. Available from: http://beast.community/treeannotator (accessed 2 October 2018)

    Rambaut, A., Suchard, M.A., Xie, D. & Drummond, A.J. (2014) Tracer. Version 1.6. Available from: http://tree.bio.ed.ac.uk/software/tracer/ (accessed 2 October 2018)

    Roy, D. & Mushahidunnabi, M. (2001) Courtship, mating and egg-laying in Tylototriton verrucosus from the Darjeeling District of the Eastern Himalaya. Current Science, 81, 693–695.

    Rowley, J., Brown, R., Bain, R., Kusrini, M., Inger, R., Stuart, B., Wogan, G., Neang, T., Chan-ard, T., Cao, T.T., Diesmos, A., Iskandar, D.T., Lau, M., Leong, M.T., Makchai, S., Nguyen, T.Q. & Phimmachak, S. (2010) Impending conservation crisis for Southeast Asian amphibians. Biology Letters, 6, 336–338.
    https://doi.org/10.1098/rsbl.2009.0793

    Rowley, J., Shepard, C.R., Stuart, B.L., Nguyen, T.Q., Hoang, H.D., Cutajar, T.P., Wogan, G.O.U. & Phimmachak, S. (2016) Estimating the global trade in Southeast Asian newts. Biological Conservation, 199, 96–100.
    https://doi.org/10.1016/j.biocon.2016.05.001

    Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, B., Larget, L., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) Mr. Bayes 3.2: efficient Bayesian phylogenetic inference and model choice across a Large Model Space. Systematic Biology, 61, 539–542.
    https://doi.org/10.1093/sysbio/sys029

    Shen, Y.H., Jiang, J.P. & Mo, X.Y. (2012) A new species of the genus Tylototriton (Amphibia, Salamandridae) from Hunan, China. Asian Herpetological Research, 3, 21–30.
    https://doi.org/10.3724/sp.j.1245.2012.00021

    Seglie, D., Roy, D. & Giacoma, C. (2010) Sexual dimorphism and age structure in a population of Tylototriton verrucosus (Amphibia: Salamandridae) from the Himalayan Region. Copeia, 4, 600–608.

    Seglie, D., Roy, D., Giacoma, C. & Mushahiddunnab, M. (2003) Distribution and conservation of the Himalayan newt (Tylototriton verrucosus, Urodela: Salamandridae) in the Darjeeling District, West Bengal (India). Russian Journal of Herpetology, 10, 159–164.

    Sparreboom, M. (2014) Salamanders of the Old World. KNNV Publishing, Zeist, 325 pp.
    https://doi.org/10.1163/9789004285620

    Sukumaran, J. & Knowles, L.L. (2017) Multispecies coalescent delimits structure, not species. Proceedings of the National Academy of Sciences, 114, 1607–1612.
    https://doi.org/10.1073/pnas.1607921114

    Sun, S.J., Dai, Q., Dai, Z.X., Zhang, H.M., Gong, R.H., Du, J.F., Zou, H.S. & Nie, C.A. (2011) Population resource and habitat selection in summer of Black Knobby Newt (Tylototriton asperrimus) in surrounding areas of Houhe National Nature Reserve, Hubei Province, China. Chinese Journal of Ecology, 30, 2534– 2539.

    Stuart, B.L., Phimmachak, S., Sivongxay, N. & Robichaud, W.G. (2010) A new species in the Tylototriton asperrimus group (Caudata: Salamandridae) from central Laos. Zootaxa, 2650, 19–32.

    Thorpe, R.S. (1975) Quantitative handling of characters useful in snake systematics with particular reference to interspecific variation in the Ringed Snake Natrix natrix (L.). Biological Journal of the Linnean Society, 7, 27–43.
    https://doi.org/10.1111/j.1095-8312.1975.tb00732.x

    Thorpe, R.S. (1983) A review of the numerical methods for recognizing and analysing racial differentiation. In: Felsenstein, J. (Ed.), Numerical Taxonomy. NATO ASI Series. Series G: Ecological Sciences. Vol. 1. Springer-Verlag, Berlin, 404–423 pp.
    https://doi.org/10.1007/978-3-642-69024-2_43

    Tian, Y., Su, A. & Li, S. (1998) Studies on reproductive ecology of Tylototriton kweichowensis Fang and Chang. Sichuan Journal of Zoology, 17, 60–64.

    Turan, C. (1999) A note on the examination of morphometric differentiation among fish populations: The Truss System. Turkish Journal of Zoology, 23, 259–263.

    Wang, B., Nishikawa, K., Masafumi, M., Nguyen, T.Q., Xie, F., Li, C., Khatiwada, J.R., Zhang, B., Gong, D., Mo, Y., Gang, W. Chen, X. Shen, Y., Yang, D., Xiong, R. & Jiang, J. (2018) Phylogenetic surveys on the newt genus Tylototriton sensu lato (Salamandridae, Caudata) reveal cryptic diversity and novel diversification promoted by historical climatic shifts. PeerJ, 6, e4384.
    https://doi.org/10.7717/peerj.4384

    Wangyal, J.T. & Gurung, D.B. (2012) The distribution of Himalayan newts, Tylototriton verrucosus in the Punakha-Wangdue Valley, Bhutan. Journal of Threatened Taxa, 4, 3218–3222.
    https://doi.org/10.11609/jott.o3136.3218-22

    Wilcox, T.P., Zwickl, D.J., Heath, T.A. & Hillis, D.M. (2002) Phylogenetic relationships of the Dwarf Boas and a comparison of Bayesian and bootstrap measures of phylogenetic support. Molecular Phylogenetics and Evolution, 25, 361–371.
    https://doi.org/10.1016/S1055-7903(02)00244-0

    Yang, D., Jiang, J., Shen, Y. & Fei, D. (2014) A new species of the genus Tylototriton (Urodela: Salamandridae) from northeastern Hunan Province, China. Asian Herpetological Research, 5, 1–11.
    https://doi.org/10.3724/sp.j.1245.2014.00001

    Yu, P.J. & Zhao, E.M. (2005) A general review of research on Echinotriton and Tylototriton in China. Sichuan Journal of Zoology, 24, 646–650.

    Yuan, Z.-Y., Jiang, K., Lü, S.-Q., Yang, J.-X., Nguyen, T.Q., Nguyen, T.T., Jin, J.-Q. & Che, J. (2011) A phylogeny of the Tylototriton asperrimus group (Caudata: Salamandridae) based on a mitochondrial study: suggestions for a taxonomic revision. Zoological Research, 32, 577–584.

    Zhang, M.-W., Rao, D.-Q., Yu, G.-H. & Yang, J.-X. (2007) The validity of red knobby newt (Tylototriton shanjing) species status based on mitochondrial Cyt b gene. Zoological Research, 28, 430–436.

    Zhang, M., Yu, G., Rao, D., Huang, Y., Yang, J. & Li, Y. (2013) A species boundary within the Tylototriton verrucosus group (Urodela: Salamandroidae [sic]) based on mitochondrial DNA sequence evidence. Journal of Animal and Veterinary Advances, 12, 337–343

    Ziegler, T., Hartmann, T., Straetena, K.V., Karbea, D. & Böhme, W. (2008) Captive breeding and larval morphology of Tylototriton shanjing Nussbaum, Brodie & Yang, 1995, with an updated key of the genus Tylototriton (Amphibia: Salamandridae). Der Zoologische Garten, 77, 246–260.
    https://doi.org/10.1016/j.zoolgart.2008.01.001