Abstract
A new species of aeglid crab, Aegla okora n. sp., is described. This species was found in the Iguaçu River basin in Paraná state, southern Brazil. Aegla okora n. sp. was discriminated based on morphological and molecular data (mitochondrial COI). Morphologically, the new species differs from congeners of the same river basin by the presence of a trapezoidal areola and the ventromesial border of the ischium having three tubercles, besides other exclusive additional morphological characteristics. Molecular results demonstrated that there is no overlap between the intraspecific distances of Aegla okora n. sp. and the interspecific distance of other species of Aegla added to the analysis, confirming the separation of species and increasing the known diversity of the Iguaçu River basin.
References
Bahamonde, N. & López, M.T. (1961) Estudios biologicos en la población de Aegla laevis laevis (Latreille) de el Monte (Crustacea, Decapoda, Anomura). Investigaciones Zoologicas Chilenas, 7, 19–58.
Bond-Buckup, G. (2003) A família Aeglidae. In: Melo, G.A.S. (Ed.), Manual de Identificação dos Crustacea Decapoda de Água Doce do Brasil. Loyola, São Paulo, pp. 21–116.
Bond-Buckup, G. & Buckup, L. (1994) A família Aeglidae (Crustacea, Decapoda, Anomura). Arquivos de Zoologia, 32, 159–346.
https://doi.org/10.11606/issn.2176-7793.v32i4p159-346
Bond-Buckup, G., Jara, C.G., Perez-Losada, M., Buckup, L. & Crandall, K.A. (2008) Global diversity of crabs (Aeglidae: Anomura: Decapoda) in freshwater. Hydrobiologia, 595, 267–273.
https://doi.org/10.1007/s10750-007-9022-4
Bond-Buckup, G., Jara, C.G., Buckup, L., Pérez-Losada, M., Bueno, A.A.P., Crandall, K.A. & Santos, S. (2010) New species and new records of endemic freshwater crabs from the Atlantic forest in Southern Brazil (Anomura: Aeglidae). Journal of Crustacean Biology, 30, 495–502.
https://doi.org/10.1651/09-3186.1
Bouckaert, R., Heled, J., Kuhnert, D., Vaughan, T., Wu, C.-H., Xie, D., Suchard, M.A., Rambaut, A. & Drummond, A.J. (2014) Beast 2: A Software Plataform for Bayesian Evolutionary Analysis. PLoS Computational Biology, 10, e1003537.
https://doi.org/10.1371/journal.pcbi.1003537
Bueno, S.L.S., Camargo, A.L. & Moraes, J.C.B. (2017) A new species of stygobitic aeglid from lentic subterranean waters in southeastern Brazil, with an unusual morphological trait: short pleopods in adult males. Nauplius, 25, 1–18.
https://doi.org/10.1590/2358-2936e2017021
Clare, E.L., Lim, B.K., Fenton, M.B. & Hebert, P.D.N. (2011) Neotropical bats: estimating species diversity with DNA barcodes. PLoS ONE, 6, e22648.
https://doi.org/10.1371/journal.pone.0022648
Crawford, A.J., Cruz, C., Griffith, E., Ross, H., Ibánez, R., Lips, K.R. & Crump, P. (2013) DNA barcoding applied to ex situ tropical amphibian conservation programme reveals cryptic in captive populations. Molecular Ecology Resources, 13, 1005–1018.
Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9, 772.
https://doi.org/10.1038/nmeth.2109
DeSalle, R., Egan, M.G. & Siddall, M. (2005) The unholy trinity: Taxonomy, species delimitation and DNA barcoding. Philosophical Transactions of the Royal Society B: Biological Sciences, 360, 1905–1916.
https://doi.org/10.1098/rstb.2005.1722
Drummond, A.J., Suchard, M.A., Xie, D. & Rambaut, A. (2012) Bayesian phylogenetics with BEAUti and the BEAST, 1.7. Molecular biology and evolution, 29, 1969–1973.
https://doi.org/10.1093/molbev/mss075
Frantine-Silva, W., Sofia, S.H., Orsi, M.L. & Almeida, F.S. (2015) DNA barcoding of freshwater ichthyoplankton in the Neotropics as a tool for ecological monitoring. Molecular Ecology Resources, 15, 1226–1237.
https://doi.org/10.1111/1755-0998.12385
Fujisawa, T. & Barraclough, T.G. (2013) Delimiting Species Using Single-Locus Data and the Generalized Mixed Yule Coalescent Approach: A Revised Method and Evaluation on Simulated Data Sets. Systematic Biology, 62, 707–724.
https://doi.org/10.1093/sysbio/syt033
Hebert, P.D.N., Cywinska, A, Ball, S.L. & de Waard, J.R. (2003) Biological identifications through DNA barcodes. Proceedings of the Royal Society, 270, 313–322.
https://doi.org/10.1098/rspb.2002.2218
Hebling, N.J. & Rodrigues, W. (1977) Sobre uma nova espécie brasileira do gênero Aegla Leach, 1820 (Decapoda, Anomura). Papéis Avulsos de Zoologia, 30, 289–294.
Hobbs III, H.H. (1978) A new species of the endemic South American genus Aegla from Paraná, Brazil (Crustacea, Anomura, Aeglidae). Proceedings of the Biological Society of Washington, 91, 982–988.
Jara, C.G., Pérez-Losada, M. & Crandall, K.A. (2003) A new species of freshwater anomuran crab of the genus Aegla Leach, 1821 (Crustacea: Decapoda: Aeglidae) from the Nahuelbuta Coastal Range, Chile. Proceedings of the Biological Society of Washington, 116, 933–942.
Johnsen, A., Rindal, E., Ericson, P.G.P., Zuccon, D., Kerr, K.C.R., Stoeckle, M.Y. & Lifjeld, J.T. (2010) DNA barcoding of Scandinavian birds reveals divergent lineages in trans-Atlantic species. Journal of Ornithology, 15, 565–578.
https://doi.org/10.1007/s10336-009-0490-3
Kimura, M. (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16, 111–120.
https://doi.org/10.1007/BF01731581
Leach, W.E. (1820) Galatéadées. In: Levrault, F.G. (Ed.), Dictionnaire des Sciences Naturelles, Strasbourg, 1820, pp. 49–56.
Lefébure, T., Douady, C.J., Gouy, M. & Gibert, J. (2006) Relationship between morphological taxonomy and molecular divergence within Crustacea: proposal of a molecular threshold to help species delimitation. Molecular Phylogenetics and Evolution, 40, 435–447.
https://doi.org/10.1016/j.ympev.2006.03.014
Li, W.L.S. & Drummond, A.J. (2012) Model averaging and Bayes factor calculation of relaxed molecular clocks in Bayesian phylogenetics. Molecular Biology and Evolution, 29, 751–761.
https://doi.org/10.1093/molbev/msr232
López, M.T. (1965) Estudios biológicos en Aegla odebrechtti paulensis, Schmitt (Crustacea, Decapoda, Anomura). Boletim da Faculdade de Filosofia, Ciências e Letras da Universidade de São Paulo, Série Zoologia, 25, 301–314.
https://doi.org/10.11606/issn.2526-3382.bffclzoologia.1965.120683
Martin, J.W. & Abele, L.G. (1986) Phylogenetic relationships of the genus Aegla (Decapoda: Anomura: Aeglidae), with comments on anomuran phylogeny. Journal of Crustacean Biology, 6, 576–616.
https://doi.org/10.2307/1548195
Monaghan, M.T., Wild, R., Elliot, M., Fujisawa, T., Balke, M., Inward, D.J., Less, D.C., Ranaivosolo, R., Eggleton, P., Barraclough, T.G. & Vogler, A.P. (2009) Accelerated species inventory on Madagascar using coalescent-based models of species delineation. Systematic Biology, 58, 298–311.
https://doi.org/10.1093/sysbio/syp027
Moraes, J.C.B., Terossi, M., Buranelli, R.C., Tavares, M., Mantelatto, F.L. & Bueno, S.L.S. (2016) Morphological and molecular data reveal the cryptic diversity among populations of Aegla paulensis (Decapoda, Anomura, Aeglidae), with descriptions of four new species and comments on dispersal routes and conservation status. Zootaxa, 4193 (1), 1–48.
https://doi.org/10.11646/zootaxa.4193.1.1
Nguyen, S.N., Yang, J.X., Le, T.N.T., Nguyen, L.T., Orlov, N.L., Hoang, C.V. & Che, J. (2014) DNA barcoding of Vietnamese bent-toed geckos (Squamata: Gekkonidae: Cyrtodactylus) and the description of a new species. Zootaxa, 3784 (1), 48–66.
https://doi.org/10.11646/zootaxa.3784.1.2
Pereira, L.H.G., Hanner, R., Foresti, F. & Oliveira, C. (2013) Can DNA barcoding accurately discriminate megadiverse Neotropical freshwater fish fauna? BMC Genetics, 14, 20.
https://doi.org/10.1186/1471-2156-14-20
Pérez-Losada, M., Jara, C.G., Bond-Buckup, G. & Crandall, K.A. (2004) Molecular systematics and biogeography of the southern South American freshwater ‘‘crabs’’ Aegla (Decapoda: Anomura: Aeglidae) using multiple heuristic tree search approaches. Systematic Biology, 53, 767–780.
https://doi.org/10.1080/10635150490522331
Pérez-Losada, M., Jara, C.G., Bond-Buckup, G. & Crandall, K.A. (2002) Phylogenetic relationships among the species of Aegla (Anomura: Aeglidae) freshwater crabs from Chile. Journal of Crustacean Biology, 22, 304–313.
https://doi.org/10.1163/20021975-99990237
Pons, J., Barraclough, T.G., Gomez-Zurita, J., Cardoso, A., Duran, D.P., Hazell, S., Kamoun, S., Sumlin, W.D. & Vogler, A.P. (2006) Sequence-based species delimitation for the DNA taxonomy of undescribed insects. Systematics Biology, 55, 595–609.
https://doi.org/10.1080/10635150600852011
Rambaut, A., Suchard, M.A., Xie, D. & Drummond, A.J. (2018) Tracer. Version 1.6. 2014. Available from: http://tree.bio.ed.ac.uk/software/tracer/ (acessed 5 July 2018)
Raupach, M.J. & Radulovici, A.E. (2015) Looking back on a decade of barcoding crustaceans. Zookeys, 539, 53.
https://doi.org/10.3897/zookeys.539.6530
Rodrigues, W. & Hebling, N.J. (1978) Estudos biológicos em Aegla perobae Hebling and Rodrigues, (1977) (Decapoda, Anomura). Revista Brasileira de Biologia, 38, 383–390.
Santos, S., Bond-Buckup, G., Pérez-Losada, M., Bartholomei-Santos, M.L. & Buckup, L. (2009) Aegla manuinflata, a new species of freshwater anomuran (Decapoda: Anomura: Aeglidae). Zootaxa, 2088, 31–40.
Santos, S., Bond-Buckup, G., Pérez-Losada, M., Jara, C.G., Crandall, K.A. & Buckup, L. (2010) New records and description of a new species of Aeglidae (Crustacea: Anomura) from river basins in Southern Brazil. Nauplius, 18, 79–86.
Santos, S., Bond-Buckup, G., Buckup, L., Pérez-Losada, M., Finley, M. & Crandall, K.A. (2012) Three new species of Aegla (Anomura) freshwater crabs from the Upper Uruguay River hydrographic basin in Brazil. Journal of Crustacean Biology, 32, 529–540.
https://doi.org/10.1163/193724012X635935
Santos, S., Jara, C.G., Bartholomei-Santos, M.L., Pérez-Losada, M. & Crandall, K.A. (2013) New species and records of the genus Aegla Leach, 1820 (Crustacea, Anomura, Aeglidea) from the West-Central region of Rio Grande do Sul, Brazil. Nauplius, 21, 211–223.
https://doi.org/10.1590/S0104-64972013000200008
Santos, S., Bond-Buckup, G., Buckup, L., Loureiro, T.G., Gonçalves, A.S., Verdi, A. & Scarabino, F. & Clavijo, C. (2014) The Aeglidae of Uruguay (Decapoda, Anomura), with the description of a new species of Aegla. In: Yeo, D.C.J., Cumberlidge, N. & Klaus, S. (Eds.), Advances in freshwater decapod systematics and biology. Crustaceana Monographs, 19, pp. 195–205.
Santos, S., Bond-Buckup, G., Buckup, L., Bartholomei-Santos, M.L., Pérez-Losada, M., Jara, C.G. & Crandall, K.A. (2015) Three new species of Aeglidae (Aegla Leach, 1890) from Paraná state, Brazil. Journal of Crustacean Biology, 35, 839–849.
https://doi.org/10.1163/1937240X-00002376
Santos, S., Bond-Buckup, G., Gonçalves, A.S., Bartholomei-Santos, M.L., Buckup, L. & Jara, C.G. (2017) Diversity and conservation status of Aegla spp. (Anomura, Aeglidae): an update. Nauplius, 25, 1–14.
https://doi.org/10.1590/2358-2936e2017011
Schmitt, W.L. (1942) The species of Aegla, endemic South American freshwater crustaceans. Proceedings of the United States National Museum, 91, 431–524.
https://doi.org/10.5479/si.00963801.91-3132.431
Souza-Shibatta, L., Tonini, J.F.R., Abrahão, V.P., Jarduli, L.R., Oliveira, C., Malabarba, L.R. & Shibatta, O.A. (2018) Reappraisal of the systematics of Microglanis cottoides (Siluriformes, Pseudopimelodidae), a catfish from southern Brazil. PLoS ONE, 13 (7), e 0199963.
https://doi.org/10.1371/journal.pone.0199963
Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729.
https://doi.org/10.1093/molbev/mst197
Trevisan, A. & Masunari, S. (2010) Geographical distribution of Aegla schmitti Hobbs III, 1979 (Decapoda Anomura Aeglidae) and morphometric variation in male population from Paraná State, Brazil. Nauplius, 18–55.
Van Syoc, R.J. (1995) Barnacle mitochondrial DNA: determining genetic relationships among species of Pollicipes. In: Schram, F.R. & Høeg, J.T. (Eds.), New Frontiers in Barnacle Evolution. Crustacean Issues. Vol. 10. A.A. Balkema, Rotterdam, pp. 269–296.