Abstract
Imparipecten, a previously monotypic genus, was considered endemic to Australia. Here, we report Imparipecten from the Neotropical region for the first time and describe Imparipecten sychnacanthus sp. n. from Brazil. The association between larvae and adults was established by sequencing a fragment of one ribosomal gene (28S), two fragments of a nuclear protein-coding gene (CAD1 and CAD4), and one mitochondrial protein-coding gene (COI). We also show the close molecular proximity with Imparipecten pictipes through analyses of genetic distances and Bayesian phylogenetics.
References
Allegrucci, G., Carchini, V.T., Convey, P. & Sbordoni, V. (2006) A molecular phylogeny of Antarctic Chironomidae and its implications for biogeographical history. Polar Biology, 29, 320–326.
https://doi.org/10.1007/s00300-005-0056-7
Anderson, A., Stur, E. & Ekrem, T. (2013) Molecular and morphological methods reveal cryptic diversity and three new species of Nearctic Micropsectra (Diptera:Chironomidae). Freshwater Science, 32 (3), 892–921.
https://doi.org/10.1899/12-026.1
Brodin, Y., Ejdung, G., Strandberg, J. & Lyrholm, T. (2013) Improving environmental and biodiversity monitoring in the Baltic Sea using DNA barcoding of Chironomidae (Diptera). Molecular Ecology Resources, 13, 996–1004.
https://doi.org/10.1111/1755-0998.12053
Cranston, P.S. & Hardwick, R. (1996) The immature stages and phylogeny of Imparipecten Freeman, an Australian endemic genus of wood-mining chironomid (Diptera). Aquatic Insects, 18, 193–207.
https://doi.org/10.1080/01650429609361623
Cranston, P.S., Hardy, N.B. & Morse, G.E. (2012) A dated molecular phylogeny for the Chironomidae (Diptera). Systematic Entomology, 37, 172–188.
https://doi.org/10.1111/j.1365-3113.2011.00603.x
Dantas, G.P.S., Hamada, N. & Mendes, H. (2010) A new Neotropical species of the genus Stenochironomus Kieffer (Diptera: Chironomidae) with wood-mining larvae. Zootaxa, 2490, 47–54.
Dantas, G.P.S., Hamada, N. & Mendes, H. (2016) Contribution to the knowledge of Stenochironomus Kieffer (Diptera, Chironomidae) from Brazil: seven new species and description of females and immatures of some previously known species. Zootaxa, 4117 (1), 1–47.
https://doi.org/10.11646/zootaxa.4117.1.1
Drummond, A.J., Rambaut, A. & Suchard, M.A. (2012) Bayesian phylogenetics with BEAUti and the BEAST 1.8.2. Molecular Biology and Evolution, 29 (8), 1969–1973.
https://doi.org/10.1093/molbev/mss075
Epler, J.H. (1988) Biosystematics of the genus Dicrotendipes Kieffer, 1913 (Diptera: Chironomidae: Chironominae) of the world. Memoirs of the American Entomological Society, 36, 1–214.
Ekrem, T., Stur, E. & Hebert, P.D.N. (2010) Females do count: Documenting Chironomidae (Diptera) species diversity using DNA barcoding. Organisms Diversity & Evolution, 10 (5), 397–408.
https://doi.org/10.1007/s13127-010-0034-y
Freeman, P. (1961) The Chironomidae (Diptera) of Australia. Australian Journal of Zoology, 9 (4), 611–737.
https://doi.org/10.1071/ZO9610611
Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.
Kimura, M. (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16 (2), 111–120.
https://doi.org/10.1007/BF01731581
Krosch, M.N., Baker, A.M., Mather, P.B. & Cranston, P.S. (2011) Systematics and biogeography of the Gondwanan Orthocladiinae (Diptera: Chironomidae). Molecular Phylogenetics and Evolution, 59 (2), 458–468.
https://doi.org/10.1016/j.ympev.2011.03.003
Langton, P.H. (1994) If not ‘filaments’ then what? Chironomus Newsletter on Chironomidae Research, 6, 9.
Lin, X., Stur, E. & Ekrem, T. (2015) Exploring Genetic Divergence in a Species-Rich Insect Genus Using 2790 DNA Barcodes. PLoS ONE, 10 (9), 1–24.
https://doi.org/10.1371/journal.pone.0138993
Lin, X., Stur, E. & Ekrem, T. (2017) DNA barcodes and morphology reveal unrecognized species in Chironomidae (Diptera). Insect Systematics & Evolution, 49 (4), 329–398. [published online]
https://doi.org/10.1163/1876312X-00002172
Lin, X., Stur, E. & Ekrem, T. (2018a) Exploring species boundaries with multiple genetic loci using empirical data from non-biting midges. Zoologica Scripta, 1–17. [published online]
https://doi.org/10.1111/zsc.12280
Lin, X., Stur, E. & Ekrem, T. (2018b) Molecular phylogeny and temporal diversification of Tanytarsus van der Wulp (Diptera: Chironomidae) support generic synonymies, a new classification and centre of origin. Systematic Entomology, 1–19. [published online]
https://doi.org/10.1111/syen.12292
Martin, J., Blinov, A., Alieva, E. & Hirabayashi, K. (2007) A molecular phylogenetic investigation of the genera closely related to Chironomus Meigen (Diptera: Chironomidae). In: Andersen, T. (Ed.), Contributions to the Systematics and Ecology of Aquatic Diptera - A Tribute to Ole A. Sæther. The Caddis press, Columbus, Ohio, pp. 193–203.
Miller, K.B., Alarie, Y., Wolfe, G.W. & Whiting, M.F. (2005) Association of insect life stages using DNA sequences: the larvae of Philodytes umbrinus (Motschulsky) (Coleoptera: Dytiscidae). Systematic Entomology, 30, 499–509.
https://doi.org/10.1111/j.1365-3113.2005.00320.x
Montagna, M., Mereghetti, V., Lencioni, V. & Rossaro, B. (2016) Integrated Taxonomy and DNA Barcoding of Alpine Midges (Diptera: Chironomidae). PLoS ONE, 11 (7), e0159124, 1-20.
https://doi.org/10.1371/journal.pone.0149673
Pinho, L.C., Mendes, H.F. & Andersen, T. (2013) Revision of Beardius Reiss et Sublette, 1985 (Diptera: Chironomidae), with the description of twenty new species. Zootaxa, 3742 (1), 1–78.
https://doi.org/10.11646/zootaxa.3742.1.1
Pinho, L.C. & Souza, J.F. (2013) New species of Xestochironomus Sublette et Wirth, 1972 (Diptera: Chironomidae) from Atlantic Forest, Brazil. Zootaxa, 3652 (5), 595–600.
https://doi.org/10.11646/zootaxa.3652.5.9
Proulx, I., Martin, J., Carew, M. & Hare, L. (2013) Using various lines of evidence to identify Chironomus species (Diptera: Chironomidae) in eastern Canadian lakes. Zootaxa, 3741 (4), 401–458.
https://doi.org/10.11646/zootaxa.3741.4.1
Platt, R.W., Woodhall, R.W. & George, A.L. (2007) Improved DNA sequencing quality and efficiency using an optimized fast cycle sequencing protocol. Biotechniques, 43 (1), 58–62.
https://doi.org/10.2144/000112499
Rambaut, A., Suchard, M.A., Xie, D. & Drummond A.J. (2014) Tracer. Version 1.6. Available from: http://beast.bio.ed.ac.uk/Tracer (accessed 18 September 2018)
Sæther, O.A. (1969) Some Nearctic Podonominae, Diamesinae, and Orthocladiinae (Diptera: Chironomidae). Bulletin—Fisheries Research Board of Canada, 170, 1–154.
Sæther, O.A. (1980) Glossary of chironomid morphology terminology (Diptera: Chironomidae). Entomologica Scandinavica Supplements, 14, 1–51.
Sæther, O.A. & Ekrem, T. (2003) Biogeography of Afrotropical Chironomidae (Diptera), with special reference to Gondwanaland. Cimbebasia, 19, 123–139.
Silva, F.L., Ekrem, T. & Fonseca-Gessner, A.A. (2013) DNA barcodes for species delimitation in Chironomidae (Diptera): a case study on the genus Labrundinia. The Canadian Entomologist, 145 (6), 589–602.
https://doi.org/10.4039/tce.2013.44
Sinclair, C.S. & Gresens, S.E. (2008) Discrimination of Cricotopus species (Diptera: Chironomidae) by DNA barcoding. Bulletin of Entomological Research, 98 (6), 555–563.
https://doi.org/10.1017/S0007485308005865
Stur, E. & Ekrem, T. (2011) Exploring unknown life stages of Arctic Tanytarsini (Diptera: Chironomidae) with DNA barcoding. Zootaxa, 2743, 27–39.
Tamura, K., Stecher, G., Peterson D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 30 (12), 2725–2729.
https://doi.org/10.1093/molbev/mst197