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Type: Article
Published: 2019-01-22
Page range: 58–70
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A new Epeorus (Caucasiron) (Ephemeroptera: Heptageniidae) species from Turkey based on molecular and morphological evidence

Biology Centre of the Czech Academy of Sciences, Institute of Entomology, Branišovská 31, CZ–37005 České Budějovice, Czech Republic. Faculty of Sciences, University of South Bohemia, Branišovská 31, CZ–37005 České Budějovice, Czech Republic
Biology Centre of the Czech Academy of Sciences, Institute of Entomology, Branišovská 31, CZ–37005 České Budějovice, Czech Republic.
Department of Biology, Faculty of Science, Hacettepe University, 06800 Beytepe—Ankara, Turkey.
Biology Centre of the Czech Academy of Sciences, Institute of Entomology, Branišovská 31, CZ–37005 České Budějovice, Czech Republic. State Museum of Natural History, National Academy of Sciences of Ukraine, Teatralna 18, UA–79008 Lviv, Ukraine.
Ephemeroptera taxonomy mayflies new species Caucasus barcoding

Abstract

Epeorus (Caucasiron) turcicus sp. nov. is described based on larvae from NE Turkey. The new species can be distinguished from other Caucasiron by a unique combination of several diagnostic characters: the presence of a rounded hypodermal medial femur spot, colouration of abdominal terga and sterna, narrow gill plate VII, fine hair-like setae on the surface of abdominal terga, and absence of postero-lateral projections on tergum X. In addition to morphological analysis, two single-locus analytical approaches are employed for delimiting the new species using COI sequences (Automatic Barcode Gap Discovery, ABGD; and General Mixed Yule Coalescent Model, GMYC). Both approaches unambiguously recognized E. (C.) turcicus sp. nov. as a distinct species. Our molecular dataset contains all Caucasiron species occurring in the Caucasus and the delimitation of individual species mostly follows the morphologically defined species. This study confirms the suitability of the GMYC approach for species delimitation within Caucasiron.

 

References

  1. Aydınlı, C. (2017) Contribution to the Knowledge of Ephemeroptera (Insecta) of the Eastern Black Sea Region. Journal of Entomological Research Society, 19 (3), 95–107.

    https://doi.org/10.11646/zootaxa.4074.1.1

    Braasch, D. (1978) Epeorus znojkoi Tshernova und Iron magnus n. sp. (Heptageniidae, Ephemeroptera) aus dem Kaukasus. Entomologische Nachrichten, 22 (5), 65–70.

    Braasch, D. (1979) Beitrag zur Kenntnis der Gattung Iron Eaton im Kaukasus (UdSSR) (III) (Ephemeroptera, Heptageniidae). Reichenbachia, 17 (33), 283–294.

    Braasch, D. (1980) Beitrag zur Kenntnis der Gattung Iron Eaton (Heptageniidae, Ephemeroptera) im Kaukasus (UdSSR), 2. Entomologische Nachrichten, 24 (10–11), 166–173.

    Braasch, D. (1981) Eintagsfliegen aus Anatolien und Iran (Ephemeroptera, Insecta). Faunistische Abhandlungen Staatliches Museum für Tierkunde in Dresden, 8, 75–79.

    Braasch, D. (2006) Neue Eintagsfliegen der Gattungen Epeorus und Iron aus dem Himalaja (Ephemeroptera, Heptageniidae). Entomologische Nachrichten und Berichte, 50 (1–2), 79–88.

    Braasch, D. & Soldán, T. (1979) Neue Heptageniidae aus Asien (Ephemeroptera). Reichenbachia, 17 (31), 261–272.

    Braasch, D. & Zimmermann, W. (1979) Iron sinitshenkovae sp.n.—eine neue Heptageniide (Ephemeroptera) aus dem Kaukasus. Entomologische Nachrichten, 23 (7), 103–107.

    Bryson, R.W., Warren, E., Savary, W.E. & Prendini, L. (2013) Biogeography of scorpions in the Pseudouroctonus minimus complex (Vaejovidae) from south-western North America: implications of ecological specialization for pre-Quaternary diversification. Journal of Biogeography, 40, 1850–1860.

    https://doi.org/10.1111/jbi.12134

    Chen, P., Wang, Y-Y. & Zhou, C-F. (2010) A New Mayfly Species of Epeorus (Caucasiron) from Southwestern China (Ephemeroptera: Heptageniidae). Zootaxa, 2527, 61–68.

    https://doi.org/10.5281/ZENODO.196454

    Davies, T.D., Tranter, M., Blackwood, I.L. & Abrahams, P.W. (1993) The character and causes of pronounced snowmelt-induced “acide episode” in a stream in a Scottish subarctic catchment. Journal of Hydrology, 146, 267–300.

    Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (2004) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3 (5), 294–299.

    Fujisawa, T. & Barraclough, T. G. (2013) Delimiting Species Using Single-Locus Data and the Generalized Mixed Yule Coalescent Approach: A Revised Method and Evaluation on Simulated Data Sets. Systematic Biology, 62 (5), 707–724.

    https://doi.org/10.1093/sysbio/syt033

    Hrivniak, Ľ., Sroka, P., Godunko, R.J. & Žurovcová, M. (2017) Mayflies of the genus Epeorus Eaton, 1881 s.l. (Ephemeroptera: Heptageniidae) from the Caucasus Mountains: a new species of Caucasiron Kluge, 1997 from Georgia and Turkey. Zootaxa, 4341 (3), 353–374.

    https://doi.org/10.11646/zootaxa.4341.3.2

    Jacks, G., Olofsson, E. & Werme, G. (1986) An acid surge in a wellbuffered stream. Ambio, 15 (5), 282–285.

    Kazancı, N. (1986) New Ephemeroptera records from Turkey. Zoology in the Middle East, 1, 141–143.

    https://doi.org/ 10.1080/09397140.1986.10637539

    Kazancı, N. (2001) Türkiye Ephemeroptera (Insecta) Faunası. Türkiye İç Suları, Araştırma Dizisi IX, İmaj Yayınevi, Ankara, 72 pp.

    Kazancı, N. (2009a) Ephemeroptera (Insecta) Fauna of Turkey: Records from Eastern Anatolia (Turkey). Review of Hydrobiology, 2, 187–195.

    Kazancı, N. (2009b) Records of Plecoptera (Insecta) species and affects of episodic acidification on physico-chemical properties of their habitats in the Eastern Black Sea Region and Yeşilırmak River Basin (Turkey). Review of Hydrobiology, 2 (2), 197–206.

    Kazancı, N. & Braasch, D. (1988) On some new Heptageniide (Ephemeroptera) from Anatolia. Faunistische Abhandlungen Staatliches Museum für Tierkunde in Dresden, 15, 131–135.

    Kazancı, N. & Türkmen, G. (2016) Comments on “An annotated catalogue of the mayfly fauna of Turkey (Insecta, Ephemeroptera)”. Review of Hydrobiology, 9 (2), 85–121.

    Kazancı, N., Türkmen, G., Başören, Ö. & Ekingen, P. (2016) TR-BMWP (Turkish-BMWP) biotic index. Review of Hydrobiology, 9 (2), 147–151.

    Katoh, K., Misawa, K., Kuma, K. & Miyata, T. (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Research, 30 (14), 3059–3066.

    https://doi.org/10.1093/nar/gkf436

    Kearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., Buxton, S., Cooper, A., Markowitz, S., Duran, C., Thierer, T., Ashton, B., Mentjies, P. & Drummond, A. (2012) Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics, 28 (12), 1647–1649.

    https://doi.org/10.1093/bioinformatics/bts199

    Kluge, N.J. (2004) The phylogenetic system of Ephemeroptera. Springer, Dordrecht, 456 pp.

    https://doi.org/10.1007/978-94-007-0872-3

    Kluge, N.J. (2015) Central Asian mountain Rhithrogenini (Ephemeroptera: Heptageniidae) with pointed and ephemeropteroid claws in the winged stages. Zootaxa, 3994 (3), 301–353.

    https://doi.org/10.11646/zootaxa.3994.3.1

    Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Molecular Biology and Evolution, 33 (7), 1870–1874.

    https://doi.org/10.1093/molbev/msw054

    Knowles, L.L. & Carstens, B.C. (2007) Delimiting species without monophyletic gene trees. Systematic Biology, 56 (6), 887–895.

    https://doi.org/10.1080/10635150701701091

    Martynov, A., Godunko, R. & Palatov, D. (2016) Kintrishi State Nature Reserve—a hotspot for mayfly (Insecta: Ephemeroptera) diversity in Adjara (Georgia). Zoosymposia, 11, 168–173.

    https://doi.org/10.11646/zoosymposia.11.1.18

    Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. Gateway Computing Environments Workshop, 2010, 1–8.

    Ministry of Forestry and Water Affairs (2015) Legislation of Surface Water Quality Management. Resmi Gazete, Date: 15.04.2015, Serial: 29327.

    Monaghan, M.T., Wild, R., Elliot, M., Fujisawa, T., Balke, M., Inward, D.J.G., Lees, D.C., Ranaivosolo, R., Eggleton, P., Barraclough, T.G. & Vogler, A.P. (2009) Accelerated species inventory on Madagascar using coalescent-based models of species delineation. Systematic Biology, 58, 298–311.

    https://doi.org/10.1093/sysbio/syp027

    Ogden, T.H. & Whiting, M.F. (2005) Phylogeny of Ephemeroptera (mayflies) based on molecular evidence. Molecular Phylogenetics and Evolution, 37, 625–643.

    https://doi.org/10.1016/j.ympev.2005.08.008

    Pereira-da-Conceicoa, L.L., Price, B.W., Barber-James, H.M., Barker, N.P., de Moor, F.C. & Villet, M.H. (2012) Cryptic variation in an ecological indicator organism: mitochondrial and nuclear DNA sequence data confirm distinct lineages of Baetis harrisoni Barnard (Ephemeroptera: Baetidae) in southern Africa. BMC Evolutionary Biology, 12, 26.

    https://doi.org/10.1186/1471-2148-12-26

    Pons, J., Barraclough, T.G., Gomez-Zurita, J., Cardoso, A., Duran, D.P., Hazell, S., Kamoun, S., Sumlin, W.D. & Vogler, A.P. (2006) Sequence-based species delimitation for the DNA taxonomy of undescribed insects. Systematic Biology, 55, 595–609.

    https://doi.org/10.1080/10635150600852011

    Puillandre, N., Lambert, A., Brouillet, S. & Achaz, G. (2012) ABGD, Automatic Barcode Gap Discovery for primary species delimitation. Molecular Ecology, 21 (8), 1864–1877.

    https://doi.org/10.1111/j.1365-294x.2011.05239.x

    Rutschmann, S., Gattolliat, J-L., Hughes, S.J., Báez, M., Sartori, M. & Monaghan, M.T. (2014) Evolution and island endemism of morphologically cryptic Baetis and Cloeon species (Ephemeroptera, Baetidae) on the Canary Islands and Madeira. Freshwater Biology, 59 (12), 2516–2527.

    https://doi.org/10.1111/fwb.12450

    Rutschmann, S., Detering, H., Simon, S. & Funk, D.H. (2017) Colonization and diversification of aquatic insects on three Macaronesian archipelagos using 59 nuclear loci derived from a draft genome. Molecular Phylogenetics and Evolution, 107, 27–38.

    https://doi.org/10.1016/j.ympev.2016.10.007

    Salur, A., Darilmaz, M.C. & Bauernfeind, E. (2016) An annotated catalogue of the mayfly fauna of Turkey (Insecta, Ephemeroptera). ZooKeys, 620, 67–118.

    https://doi.org/10.3897/zookeys.620.9405

    Sinitshenkova, N.D. (1976) Mayflies of the Genus Iron Eaton (Ephemeroptera, Heptageniidae) in the Fauna of the Caucasus. Entomologicheskoye obozreniye, 55 (4), 853–862.

    Tarkhnishvili, D. (2014) Historical Biogeography of the Caucasus. Nova Science Publishers, New York, 234 pp.

    Tshernova, O.A. (1938) Zur Kenntnis der Ephemeropteren Ost-Transkaukasien. Trudy Azerbajdshanskogo Filiala AN SSSR, Baku, 7 (42), 55–64.

    Türkmen, G. & Kazancı, N. (2015) Additional records of Ephemeroptera (Insecta) species from the Eastern Part of Black Sea Region (Turkey). Review of Hydrobiology, 8, 33–50.

    Vuataz, L., Sartori, M., Wagner, A. & Monaghan, M.T. (2011) Toward a DNA taxonomy of Alpine Rhithrogena (Ephemeroptera: Heptageniidae) using a mixed Yule-coalescent analysis of mitochondrial and nuclear DNA. PLos ONE, 6 (5), e19728.

    https://doi.org/10.1371/journal.pone.0019728

    Vuataz, L., Sartori, M., Gattolliat, J-L. & Monaghan, M.T. (2013) Endemism and diversification in freshwater insects of Madagascar revealed by coalescent and phylogenetic analysis of museum and field collections. Molecular Phylogenetics and Evolution, 66 (3), 979–991.

    https://doi.org/10.1016/j.ympev.2012.12.003

    Yanai, I., Sartori, M., Dor, R. & Dorchin, N. (2017) Molecular phylogeny and morphological analysis resolve a long-standing controversy over generic concepts in Ecdyonurinae mayflies (Ephemeroptera: Heptageniidae). Systematic Entomology, 42 (1), 182–193.

    https://doi.org/10.1111/syen.12203

    Zaldívar-Riverón, A., Martínez, J.J., Ceccarelli, S.F., De Jesús-Bonilla, V.S., Rodríguez-Pérez, A.C., Reséndiz-Flores, A. & Smith, M.A. (2010) DNA barcoding a highly diverse group of parasitoid wasps (Braconidae: Doryctinae) from a Mexican nature reserve. Mitochondrial DNA, 21 (Supplement 1), 18–23.

    https://doi.org/10.3109/19401736.2010.523701