Abstract
Early life-history stages of 12 of 17 species of western Central Atlantic Apogon were identified using molecular data. A neighbor-joining tree was constructed from mitochondrial cytochrome oxidase-c subunit I (COl) sequences, and genetic lineages of Apogon in the tree were identified to species based on adults in the lineages. Relevant portions of the tree subsequently were used to identify larvae of Apogon species from Carrie Bow Cay, Belize, and juveniles from Belize and other western Central Atlantic localities. Diagnostic morphological characters of larvae and juveniles were investigated by examining preserved vouchers from which the DNA was extracted and digital color photographs of those specimens taken before preservation. Orange and yellow chromatophore patterns are the easiest and sometimes only means of separating Apogon larvae. Patterns of melanophores and morphometric features are of limited diagnostic value. For juveniles, chromatophore patterns and the developing dark blotches characteristic of adults are the most useful diagnostic features. Larvae were identified for Apogon aurolineatus, A. binotatus, A. maculatus, A. mosavi, A. phenax, A. planifrons, and A. townsendi. Juveniles were identified for those species (except A. planifrons) and for A. pseudomaculatus, A. lachneri, A. pillionatus, A. robbyi, and A. quadrisquamatus. One larval specimen occurs in an unidentified genetic lineage, and five adults occur in another unidentified genetic lineage. Apogon species can be divided into at least four groups based on pigmentation patterns in early life stages. Further investigation is needed to determine if those groups are meaningful in the generic classification of Apogon species.
References
Ahlstrom, E.H., Richards, W.J. & Weitzman, S.H. (1984) Families Gonostomatidae, Sternoptychidae, and associated stomiiform groups: development and relationships. In: Moser, H.G., Richards, W.J., Cohen, D. M., Fahay, M. E., Kendall Jr., A. W. & Richardson, S.L. (Eds.). Ontogeny and systematics of fishes. Special publication No. 1, American Society of Ichthyologists and Herpetologists, Lawrence, KS, pp. 184–198.
Baldwin, C.C., Mounts J.H., Smith D.G. & Lee, A.W. (2009a) Genetic identification and color descriptions of early life-history stages of Belize Phaeoptyx and Astrapogon (Teleostei: Apogonidae) with comments on identification of adult Phaeoptyx. Zootaxa, 2008, 1–22.
Baldwin, C.C., Weigt, L.A., Smith, D.G. & Mounts, J.H. 2009b Reconciling genetic lineages with species in western Atlantic Coryphopterus (Teleostei: Gobiidae). Pp. 113–140. In Lang, M.A., Macyntire, I.G. & Rützler, K. (Eds) Proceedings of the Smithsonian Marine Science Symposium. Smithsonian Contributions to the Marine Sciences No. 38. Smithsonian Institution Scholarly Press, Washington, D.C. pp. 113–138.
Baldwin, C.C., Castillo, C.I., Weigt, L. A. & Victor, B. (2011) Seven new species within western Atlantic Starksia atlantica, S. lepicoelia, and S. sluiteri (Teleostei, Labrisomidae), with comments on congruence of DNA barcodes and species. ZooKeys, 79, 21–72.
Böhlke, J.E. & Chaplin, C.C.G. (1993) Fishes of the Bahamas and Adjacent Tropical Waters. Second Edition. University of Texas Press, Austin, 771 pp.
Böhlke, J.E. & Randall, J.E. (1968) A key to the shallow-water Atlantic cardinalfishes (Apogonidae), with descriptions of five new species. Proceedings of the Academy of Natural Sciences of Philadelphia, 120, 175–206.
Dale, G. (1977) Apogon mosavi, a new Western Atlantic cardinalfish, and a note on the occurrence of Apogon leptocaulus in the Bahamas. Proceedings of the Biological Society of Washington, 90, 19–29.
Gilbert, C.R. & Tyler, J.C. (1997) Apogon robbyi a new cardinalfish (Perciformes:Apogonidae) from the Caribbean Sea. Bulletin of Marine Science, 60, 764–781.
Gon, O. (2002) Apogonidae. In: Carpenter, K. (Ed.) The living marine resources of the Western Central North Atlantic. Vol. 3: Bony fishes part 2 (Opistognathidae to Molidae), sea turtles and marine mammals. FAO Species Identification Guide for Fishery Purposes and American Society of Ichthyologists and Herpetologists Special Publication No. 5, Rome, pp. 1386–1391.
Hebert, P.D.N., Cywinska, A., Ball, S.L. & De Waard, J.R. (2003) Biological identifications through DNA barcodes. Proceedings of the Royal Society of London B. Biological Sciences, 270, 313–321.
Kimura, M. (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16, 111–120.
Lara, M.R. (2006) Apogonidae: Cardinalfishes. In: Richards, W. J. (Ed.). Early stages of Atlantic fishes: an identification guide for western central North Atlantic. Volume II, CRC Press, Boca Raton, pp. 1363–1387.
Longley, W.H. (1934) Studies on West Indian fishes: description of six new species. Carnegie Institution of Washington Year Book, 33, 257–260.
Packer, L., Gibbs, J., Sheffield, C. & Hanner, R. (2009) DNA barcoding and the mediocrity of morphology. Molecular Ecology Resources, 9, 42–50.
Pegg, G.G., Sinclair, B., Briskey, L. & Aspden, W.J. (2006) MtDNA barcode identification of fish larvae in the southern Great Barrier Reef, Australia. Scientia Marina, 70, 7–12.
Randall, J.E. & Böhlke, J.E. (1981) The status of the cardinalfishes Apogon evermanni and A. anisolepis (Perciformes: Apogonidae) with description of a related new species from the Red Sea. Proceedings of the Academy of Natural Sciences of Philadelphia, 133, 129–140.
Richards, W.J. (2006) Early stages of Atlantic fishes: an identification guide for western central North Atlantic. Volume II, CRC Press, Boca Raton, 2640 pp.
Saitou, N. & Nei, M. (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4, 406–425.
Seutin, G., White, B.N. & Boag, P.T. (1990) Preservation of avian blood and tissue samples for DNA analysis. Canadian Journal of Zoology, 69, 82–90.
Smith-Vaniz, W.F. (1977) Apogon gouldi n. sp., a new cardinalfish from Bermuda (Perciformes: Apogonidae). Notulae Naturae, 452, 1–8.
Tamura, K., Dudley, J, Nei, M & Kumar, S (2007) MEGA 4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24, 1596–1599.
Thacker, C.E. & Roje, D.M. (2009) Phylogeny of cardinalfishes (Teleostei: Gobiiformes: Apogonidae) and the evolution of visceral bioluminescence. Molecular Phylogenetics and Evolution, 52, 735–745.
Tornabene, L., Baldwin, C., Weigt, L. & Pezold, F. (2010) Exploring the diversity of Bathygobius (Teleostei: Gobiidae) using cytochrome-c oxidase subunit I, with descriptions of two new species. Aqua, International Journal of Ichthyology, 16, 141–170.
Valdez-Moreno, M., Vásquez-Yeomans, L, Elías-Gutirrez, M., Ivanova, N.V. & Hebert P.D.N. (2010) Using DNA barcodes to connect adults and early life stages of marine fishes from the Yucatan Peninsula, Mexico: potential in fisheries management. Marine and Freshwater Research, 61, 665–671.
Victor, B.C. (2007) Coryphopterus kuna, a new goby (Perciformes: Gobiidae: Gobiinae) from the western Caribbean, with the identification of the late larval stage and an estimate of the pelagic larval duration. Zootaxa, 1526, 51–61.
Victor, B.C., Hanner, R., Shivji, M., Hyde, J. & Caldow, C. (2009) Identification of the larval and juvenile stages of the Cubera Snapper, Lutjanus cyanopterus, using DNA barcoding. Zootaxa, 2215, 24–36.