Abstract
We describe the new species Amolops mengdingensis sp. nov. from southwestern Yunnan, China. It belongs to the Amolops monticola species group and phylogenetically clusters together with Amolops akhaorum and Amolops archotaphus with strong support, although the relationships between these three species are not resolved. Distance between the new species and A. akhaorum is 4.7% and distance between the new species and A. archotaphus is 5.2% for 16S rRNA sequences. The new species is characterized by the following characters: distinct dorsolateral folds; side of the head dark brown with a white upper lip stripe extending to the axilla; dorsal surface smooth, except white spinules on posterior part of dorsolateral folds and above vent; top of head and dorsum green with dark spots in life; loreal region dark brown and flanks brown without any coloration of green; SVL 36.9–40.2 mm in males and 64.3 mm in female; two external subgular vocal sacs in males; vomerine teeth in two oblique ridges, separated by a small distance; pineal body absent; snout longer than eye diameter; internarial space broader than interorbital space; tympanum distinct, smaller than half of eye diameter; all fingertips expanded; two palmar tubercles; outer metatarsal tubercle absent; tibiotarsal articulation reaching beyond snout tip; near immaculate venter; limbs dorsally grayish brown with clear dark brown bars on hindlimbs; and velvety nuptial pads.
References
Anderson, J. (1871) A list of the reptilian accession to the Indian Museum, Calcutta from 1865 to 1870, with a description of some new species. Journal of the Asiatic Society of Bengal, 40, 12–39.
Annandale, N. (1912) Zoological results of the Abor Expedition, 1911–1912. I. Amphibia. Records of the Indian Museum, 8, 7–36.
https://doi.org/10.5962/bhl.part.1186
Bain, R.H., Lathrop, A., Murphy, R.W., Orlov, N.L. & Ho, C.T. (2003) Cryptic species of a cascade frog from Southeast Asia: taxonomic revisions and descriptions of six new species. American Museum Novitates, 3417, 1–60.
https://doi.org/10.1206/0003-0082(2003)417<0001:CSOACF>2.0.CO;2
Bain, R.H. & Nguyen, T.Q. (2004) Herpetofauna diversity of Ha Giang Province in northeastern Vietnam, with descriptions of two new species. American Museum Novitates, 3453, 1–42.
https://doi.org/10.1206/0003-0082(2004)453<0001:HDOHGP>2.0.CO;2
Bain, R.H., Stuart, B.L. & Orlov, N.L. (2006) Three new Indochinese species of cascade frogs (Amphibia: Ranidae) allied to Rana archotaphus. Copeia, 2006, 43–50.
https://doi.org/10.1643/0045-8511(2006)006[0043:TNISOC]2.0.CO;2
Blyth, E. (1855) Report of the Curator; Zoological Department, for March meeting. Journal of the Asiatic Society of Bengal, 24, 187–188.
Boulenger, G.A. (1899) On the reptiles, batrachians, and fishes collected by the late Mr. John Whitehead in the interior of Hainan. Proceedings of the Zoological Society of London, 1899, 956–962.
Boulenger, G.A. (1908) A revision of the Oriental pelobatid batrachians (genus Megalophrys). Proceedings of the Zoological Society of London, 1908, 407–430.
https://doi.org/10.1111/j.1096-3642.1908.tb01852.x
Che, J., Chen, H., Yang, J., Jin, J., Jiang, K., Yuan, Z., Murphy, R.W. & Zhang, Y. (2012) Universal COI primers for DNA barcoding amphibians. Molecular Ecology Resources, 12, 247–258.
https://doi.org/10.1111/j.1755-0998.2011.03090.x
Cope, E.D. (1865) Sketch of the primary groups of Batrachia s. Salientia. Natural History Review, New Series, 5, 97–120.
David, A. (1872) Rapport adressé a MM. les Professeurs-Administráteurs du Museum d'histoire naturelle. Nouvelles Archives du Muséum d'Histoire Naturelle, 7, 75–100.
Dever, J.A., Fuiten, A.M., Konu, O. & Wilkinson, J.A. (2012) Cryptic torrent frogs of Myanmar: an examination of the Amolops marmoratus species complex with the resurrection of Amolops afghanus and the identification of a new species. Copeia, 1, 57–76.
https://doi.org/10.1643/ch-10-180
Dong, B., Rao, D. & Lü, S. (2005) Amolops aniqiaoensis. In: Zhao, W., Rao, D., Lü, S. & Dong, B. (Eds.), Herpetological surveys of Xizang autonomous region 2. Medog. Sichuan Journal of Zoology, 24, 250–253.
Fei, L. (1999) Atlas of Amphibians of China. Henan Publishing House of Science and Technol-ogy, Zhengzhou, 432 pp.
Frost, D.R. (2019) Amphibian Species of the World: and Online Reference. Version 6.0. Ameri-can Museum of Natural History, New York, USA. Available from http://research.amnh.org/herpetology/amphibia/index.html/ (accessed 15 February 2019)
Hedges, S.B. (1994) Molecular evidence for the origin of birds. Proceedings of the National Academy of Sciences of the United States of America, 91, 2621–2624.
https://doi.org/10.1073/pnas.91.7.2621
Huelsenbeck, J.P. & Ronquist, F. (2001) MrBayes: Bayesian inference of phylogeny. Bioinformatics, 17, 754–755.
https://doi.org/10.1093/bioinformatics/17.8.754
Inger, R.F. & Chan-ard, T. (1997) A new species of ranid frog from Thailand, with comments on Rana livida (Blyth). Natural History Bulletin of the Siam Society, 45, 65–70.
Jiang, K., Wang, K., Yan, F., Xie, J., Zhou, D., Liu, W., Jiang, J., Li, C. & Che, J. (2016) A new species of the genus Amolops (Amphibia: Ranidae) from southeastern Tibet, China. Zoological Research, 37, 31–40.
Jiang, Y. (1983) A new species of the genus Staurois (Ranidae), Staurois viridimaculatus. Acta Herpetologica Sinica, New Series, 2, 71.
Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33, 1870–1874.
https://doi.org/10.1093/molbev/msw054
Liu, C. (1950) Amphibians of western China. Fieldiana. Zoology Memoires, 2, 1–397.
https://doi.org/10.5962/bhl.part.4737
Liu, W. & Yang, D. (2000) A new species of Amolops (Anura: Ranidae) from Yunnan, China, with a discussion of karyological diversity in Amolops. Herpetologica, 56, 231–238.
Liu, W., Yang, D., Ferraris, C. & Matsui, M. (2000) Amolops bellulus: a new species of stream-breeding frog from western Yunnan, China (Anura: Ranidae). Copeia, 2000, 536–541.
Ohler, A. (2007) New synonyms in specific names of frogs (Raninae) from the border regions between China, Laos and Vietnam. Alytes, 25, 55–74.
Orlov, N.L. & Cuc, H.T. (2007) Two new species of cascade ranids of Amolops genus (Amphibbia: Anura: Ranidae) from Lai Chau Province (Northwest Vietnam). Russian Journal of Herpetology, 3, 211–228.
Pope, C.H. (1929) Four new frogs from Fukien Province, China. American Museum Novitates, 352, 1–5.
Posada, D. & Crandall, K.A. (1998) Modeltest: testing the model of DNA substitution. Bioinfor-matics, 14, 817–818.
https://doi.org/10.1093/bioinformatics/14.9.817
Rao, D. & Wilkinson, J.A. (2007) A new species of Amolops (Anura: Ranidae) from Southwest China. Copeia, 4, 913–919.
https://doi.org/10.1643/0045-8511(2007)7[913:ansoaa]2.0.co;2
Ray, P. (1992) Two new hill-stream frogs of the genus Amolops Cope (Amphibia: Anura: Ranidae) from Uttar Pradesh (India). Indian Journal of Forestry, 15, 346–350.
Ray, P. (1999) Systematic studies on the amphibian fauna of the District Dehradun, Uttar Pradesh, India. Memoirs of the Zoological Survey of India, 18, 1–102.
Stuart, B.L., Bain, R.H., Phimmachak, S. & Spence, K. (2010) Phylogenetic systematics of the Amolops monticola group (Amphibia: Ranidae), with description of a new species from northwestern Laos. Herpetologica, 66, 52–66.
https://doi.org/10.1655/08-073.1
Stuart, B.L., Inger, B.F. & Voris, H.K. (2006) High level of cryptic species diversity revealed by sympatric lineages of Southeast Asian forest frogs. Biology Letters, 2, 470–474.
https://doi.org/10.1098/rsbl.2006.0505
Su, C., Yang, D. & Li, S. (1986) A new species of Amolops from the Hengduan Shan Mountains. Acta Herpetologica Sinica, 5, 204–206.
Wu, G. & Tian, W. (1995) A new Amolops species from southern Yunnan. In: Zhao, E. (Ed.), Amphibian Zoogeographic Division of China. A Symposium Issued to Celebrate the Second Asian Herpetological Meeting Held at Ashgabat, Turkmenistan 6 to 10 September 1995. Sichuan Journal of Zoology, Supplement, pp. 51–52.
Ye, C., Fei, L. & Jiang, J. (2007) A new Ranidae species from China—Limnonectes bannaensis (Ranidae: Anura). Zoological Research, 28, 545–550.
Yuan, Z., Jin, J., Li, J., Btuart, B.L. & Wu, J. (2018) A new species of cascade frog (Amphibia: Ranidae) in the Amolops monticola group from China. Zootaxa, 4415 (3), 498–512.
https://doi.org/10.11646/zootaxa.4415.3.5