Abstract
The neotropical genus Physalaemus Fitzinger is currently composed by 48 species (Cardozo & Pereyra 2018). Recently, a phylogenetic analysis aiming to investigate the internal relationships of the genus recovered two major clades, the Physalaemus cuvieri and P. signifer clades (Lourenço et al. 2015). The following species groups were retrieved in the first clade: P. biligonigerus, P. cuvieri, P. gracilis, P. henselii, and P. olfersii groups (Lourenço et al. 2015). This proposal redefined the P. olfersii group including P. olfersii (Lichtenstein & Martens), P. soaresi Izecksohn, P. maximus Feio, Pombal, & Caramaschi, P. feioi Cassini, Cruz, & Caramaschi and P. lateristriga (Steindachner). The authors also allocated tentatively P. orophilus Cassini, Cruz, & Caramaschi, and P. insperatus Cruz, Cassini, & Caramaschi in the P. olfersii group due to their morphological similarity with the other species (Cruz et al. 2008; Cassini et al. 2010). Otherwise, Physalaemus aguirrei Bokermann was not recovered nested within this group, contradicting what was suggested in a previous phenetic analysis (Nascimento et al. 2005). Members of the P. olfersii group inhabit the Atlantic rainforest and most of them have a similar advertisement calls with pulsed notes, without frequency modulation and harmonic structure (Giaretta et al. 2009; Cassini et al. 2010; Lourenço et al. 2015). Regarding their larval stage, only P. soaresi, P. maximus, and P. olfersii have their tadpoles described (Weber et al. 2005; Baêta et al. 2007; Giaretta et al. 2009). Physalaemus orophilus occurs in montane Atlantic Forest sites at the eastern slope of the Espinhaço Range in the State of Minas Gerais, southeastern Brazil (Cassini et al. 2010). Herein, we describe the tadpole of P. orophilus from Quadrilátero Ferrífero mountain region, southern limit of the Espinhaço Range and compared it to the known tadpoles of the P. olfersii group.
References
Altig, R. & McDiarmid, R.W. (1999) Body plan: development and morphology. In: McDiarmid, R.W. & Altig, R. (Eds.), Tadpoles: The Biology of Anuran Larvae. The University of Chicago Press, Chicago, pp. 24–51.
Baêta, D., Lourenço, A.C.C., Pezzuti, T.L. & Pires, M.R.S. (2007) The tadpole, advertisement call, and geographic distribution of Physalaemus maximus Feio, Pombal, Caramaschi, 1999 (Amphibia, Anura, Leiuperidae). Arquivos do Museu Nacional, 65, 27–32.
Canelas, M.A.S. & Bertoluci, J. (2007) Anurans of the Serra do Caraça, southeastern Brazil: species composition and phenological patterns of calling activity. Iheringia, Série Zoologia, 97, 21–26.
https://doi.org/10.1590/S0073-47212007000100004
Cardozo, D. & Pereyra, M.O. (2018) A new species of Physalaemus (Anura, Leptodactylidae) from the Atlantic Forest of Misiones, northeastern Argentina. Zootaxa, 4387 (3), 580–590.
https://doi.org/10.11646/zootaxa.4387.3.10
Cassini, C., Cruz, C.A.G. & Caramaschi, U. (2010) Taxonomic review of Physalaemus olfersii (Lichtenstein, Martens, 1856) with revalidation of Physalaemus lateristriga (Steindachner, 1864) and description of two new related species (Anura: Leiuperidae). Zootaxa, 2491, 1–33.
https://doi.org/10.11646/zootaxa.2491.1.1
Cruz, C.A.G., Cassini, C.S. & Caramaschi, U. (2008). A new species of the genus Physalaemus Fitzinger, 1826 (Anura, Leiuperidae) from southern Brazil. South American Journal of Herpetology, 3, 239–243.
https://doi.org/10.2994/1808-9798-3.3.239
Giaretta, A.A., Martins, L.B. & Santos, M.P. (2009) Further notes on the taxonomy of four species of Physalaemus (Anura, Leiuperidae) from the Atlantic Forest of Southeastern Brazil. Zootaxa, 2266, 51–60.
Gosner, K.L. (1960) A simplified table for staging anuran embryo and larvae with notes on identification. Herpetologica, 16, 183–190.
Grosjean, S. (2005) The choice of external morphological characters and developmental stages for tadpole–based anuran taxonomy: a case study in Rana (Sylvirana) nigrovittata (Blyth, 1855) (Amphibia, Anura, Ranidae). Contributions to Zoology, 74, 61–76.
https://doi.org/10.1163/18759866-0740102005
Lavilla, E.O. & Scrocchi, G.J. (1986) Morfometría larval de los géneros de Telmatobiinae (Anura: Leptodactylidae) de Argentina y Chile. Physis, 44, 39–43.
Lins, A.C.R., De Magalhães, R.F., Costa, R.N., Brandão, R.A., Py-Daniel, T.R., Miranda, N.E.O, Maciel, N.M., Nomura, F. & Pezzuti, T.L. (2018) The larvae of two species of Bokermannohyla (Anura, Hylidae, Cophomantini) endemic to the highlands of central Brazil. Zootaxa, 4527 (4), 501–520.
https://doi.org/10.11646/zootaxa.4527.4.3
Lourenço, L.B., Targueta, C.P., Baldo, D., Nascimento, J., Garcia, P.C.A., Andrade, G.A., Haddad, C.F.B. & Recco-Pimentel, S.M. (2015) Phylogeny of frogs from the genus Physalaemus (Anura, Leptodactylidae) inferred from mitochondrial and nuclear gene sequences. Molecular Phylogenetics and Evolution, 92, 204–216.
https://doi.org/10.1016/j.ympev.2015.06.011
Nascimento, L.B., Caramaschi, U. & Cruz, C.A.G. (2005) Taxonomic review of the species group of the genus Physalaemus Fitzinger, 1826 with revalidation of the genera Engystomops Jimenéz–de–la–Espada, 1872 and Eupemphix Steindachner,1863 (Amphibia, Anura, Leptodactylidae). Arquivos do Museu Nacional, 63, 297–320.
Schacht, M.C. & McBrayer, L.D. (2009) A method for constructing an adjustable platform to obtain lateral photographs of larval anurans. Herpetological Review, 40, 303–304.
https://doi.org/10.1111/j.1467-9973.2009.01585.x
Schneider, C.A., Rasband, W.S. & Eliceiri, K.W. (2012) NIH Image to ImageJ: 25 years of image analysis. Nature Methods, 9, 671–675.
https://doi.org/10.1038/nmeth.2089
Vera Candioti, F., Haad, B., Baldo, D., Kolenc, F., Borteiro, C. & Altig, R. (2011) Different pathways are involved in the early development of the transient oral apparatus in anuran tadpoles (Anura: Leiuperidae). Biological Journal of the Linnean Society, 104, 330–345.
https://doi.org/10.1111/j.1095-8312.2011.01727.x
Weber, L.N., Carvalho e Silva, S.P. & Gonzaga, L.P. (2005) The tadpole of Physalaemus soaresi (Anura, Leptodactylidae), with comments on taxonomy, reproductive behavior, and vocalizations. Zootaxa, 1072, 35–42.
https://doi.org/10.11646/zootaxa.1072.1.3