Abstract
We compared DNA barcoding to “traditional” taxonomic tools in clarifying relationships in complexes of closely related, putative “species” of Elachistinae moths (Gelechioidea: Elachistidae) occurring in Australia. A 705 bp fragment of the 3’-end of cytochrome c oxidase subunit I gene (COI) was used. This mtDNA fragment did not differentiate between all species-level taxa that could be defined by morphological and/or ecological differences. Different evolutionary rates of COI among closely related lineages were observed. Although our findings are based on the variability of the 3’ end of the COI gene and not the 5’ end barcode fragment, we are convinced that thorough exploration of traditional morphology and ecology is a prerequisite for exploring insufficiently known taxonomies by the barcode approach. The sole use of COI barcoding, whether considering COI-5’ or COI-3’ fragment, may fail to recognize closely related species. Our results discourage this approach for delimitation of closely related species, but its use is encouraged as an additional tool for exploring little known taxonomies or as an identification tool for previously thoroughly studied species complexes.References
Albrecht, A. & Kaila, L. (1997) Variation of wing venation in Elachistidae (Lepidoptera, Gelechioidea): methodology and implications to systematics. Systematic Entomology, 22, 185–198.
Besansky, N.J., Severson, D.W. & Ferdig, M.T. (2003) DNA barcoding of parasites and invertebrate disease vectors: what you don’t know can hurt you. Trends in Parasitology, 19, 545–546.
Blaxter, M. (2003) Molecular systematics: Counting angels with DNA. Nature, 421, 122–124.
Bremer, K. (1988) The limits of amino acid sequence data in angiosperms phylogenetic reconstruction. Evolution, 42, 795–803.
Bremer, K. (1994) Branch support and tree stability. Cladistics, 10, 295–304.
Clary, D.O. & Wolstenholme, D.R. (1985) The mitochondrial DNA molecule of Drosophila yakuba. Nucleotide sequence, gene organization and geneticcode. Journal of Molecular Evolution, 22, 252–271.
Dayrat, B. (2005) Towards integrative taxonomy. Biological Journal of the Linnean Society, 85, 407–415.
Goloboff, P. (1999) NONA, vs. 2.0, parsimony program and documentation.
Hebert, P.D.N., Cywinska, A., Ball, S.L. & deWaard, J.R. (2003a) Biological identifications through DNA barcodes. Proceedings of the Royal Society of London, series B, 270, 313–321.
Hebert, P.D.N., Ratnasingham, D. & de Waard, J.R. (2003b) Barcoding animal life: cytochrome c oxidase subunit I divergences among closely related species. Proceedings of the Royal Society of London, series B (Suppl.) DOI 10.1098/rsbl.2003.0025.
Hebert, P.D.N., Penton, E.H., Burns, J.M., Janzen, D.H. & Hallwachs, W. (2004) Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proceedings of the National Academy of Sciences of the United States of America, 101, 14812–14827.
Huemer, P. & Kaila, L. (2003) Elachista (Elachista) morandinii sp. n., a new species from Central Europe (Lepidoptera, Elachistidae). Gortania, 24 (2002), 211–220.
Kaila, L. (1992) The Elachistidae of southern Siberia and Central Asia, with descriptions of five new species (Lepidoptera). Entomologica Fennica, 3, 177–194.
Kaila, L. (1996) A revision of the Nearctic Elachista s. l. I. The tetragonella group (Lepidoptera, Elachistidae). Entomologica scandinavica, 27, 217–238.
Kaila, L. (1997) A revision of the Nearctic Elachista s. l. II. The argentella group (Lepidoptera, Elachistidae). Acta Zoologica Fennica, 206, 1–93.
Kaila, L. (1998a) Redescriptions of three Meyrick’s Asiatic elachistid species, with descriptions of three new species (Lepidoptera, Gelechioidea). Entomologica Fennica, 9, 53–63.
Kaila, L. (1998b) Two new Elachista species (Lepidoptera, Elachistidae) from the Polar Urals region, Russia. Entomologica Fennica, 8, 219–223.
Kaila, L., (1999a) Phylogeny and classification of the Elachistidae s. s. (Lepidoptera, Gelechioidea). Systematic Entomology, 24, 139–169.
Kaila, L., (1999b) A revision of the Nearctic species of the Elachista s. l. III. The bifasciella, praelineata, saccharella and freyerella groups (Lepidoptera, Elachistidae). Acta Zoologica Fennica, 211, 1–235.
Kaila, L., (1999c) A review of the South-American Elachistidae s. str. (Lepidoptera, Gelechioidea) with descriptions of 15 new species. Steenstrupia, 25, 159–193.
Kaila, L. (2005) A review of Dibrachia Sinev & Sruoga, 1992, a subgenus of Elachista (E lachistidae: Elachistinae). Nota lepidopterologica, 28, 139–155.
Kaila, L., Bengtsson, B.Å., Šulcs, I. & Junnilainen, J. (2001) A revision of the Elachista regificella Sircom –complex (Lepidoptera, Elachistidae). Entomologica Fennica, 12, 153–168.
Kaila, L. & Jalava, J. (1994) Elachista adelpha sp. n., E. coeneni titanella ssp. n., and other Elachistidae (Lepidoptera) from North Caucasus. Entomologica Fennica, 5, 97–102.
Kaila, L. & Junnilainen, J. (2002) Taxonomy and identification of Elachista cingillella (H. S.) and its close relatives (Lepidoptera, Elachistidae), with descriptions of two new species. Entomologica Fennica, 13, 167–188.
Kaila, L. & Karsholt, O. (2002) Contribution to the Lepidoptera fauna of the Madeira Islands 3. Elachistidae. Beiträge zur Entomologie, 52, 225–233.
Kaila, L., Nupponen, K., Junnilainen, J., Nupponen, T., Kaitila, J.-P. & Olschwang, V. (2003) Contribution to the fauna of Elachistidae (Lepidoptera) of the Southern Ural Mountains. Entomologica Fennica, 14, 65–90.
Kaila, L. & Sugisima, K. (2003) Phylogeny of Elachistinae (Lepidoptera: Gelechioidea) revisited. Cladistics, 19, 154–155.
Kaila, L. & Varalda, P. (2004) The Elachista juliensis complex revisited (Elachistidae). Nota lepidopterologica, 27, 217–237.
Nixon, K. (2002) WinClada., Version 1.00.08. Published by the author, Ithaca, New York.
Prendini, L. (205) Comment on “identifying spiders through DNA barcodes”. Canadian Journal of Zoology, 83. 498–504.
Robinson, G.S. (1976) The preparation of slides of Lepidoptera genitalia with special reference to the Microlepidoptera. Entomologist’s Gazette, 27, 127–132.
Scoble, M.J. (1992) The Lepidoptera. Form, function and diversity. Oxford University Press, 404 pp.
Simon, C., Frati, F., Beckenbach, A., Crespi, B., Liu, H. & Flook, P. (1994) Evolution, weighting and phylogenetic utility of mitochondrial gene-sequences and a compilation of conserved polymerase chain-reaction primers. Annals of the Entomological Society of America, 87, 651–701.
Sperling, F.A.H. (2003) Butterfly molecular systematics: From species definitions to higher-level phylogenies. In: Boggs, C. L., Ehlrich, P. R. & Watt, W. B., (Ed.), Butterflies: Ecology and Evolution Taking Flight: Butterflies as Model Study Systems. University of Chicago Press, Chapter 20, pp. 431–458.
Sperling, F.A, Landry, J.-F. & Hickey, D.A. (1995) DNA-based identification of introduced ermine moth species in North America (Lepidoptera: Yponomeutidae). Annals of the Entomological Society of America, 88, 155–162.
Sugisima K. & Kaila, L. (2005) Japanese Elachista (Lepidoptera, Elachistidae s. str.) mining on the leaf of woody Poaceae. Entomologica Fennica, 16, 83–102.
Traugott-Olsen, E. & Schmidt Nielsen, E. (1977) The Elachistidae (Lepidoptera) of Fennoscandia and Denmark. Fauna Enomologica Scandinavica, 6, 1–299.
Wahlberg, N., Oliveira, R. & Scott, J.A. (2003) Phylogenetic relationships of Phyciodes butterfly species (Lepidoptera: Nymphalidae): complex mtDNA variation and species delimitations. Systematic Entomology, 28, 257–273.