Abstract
Many species of freshwater prawns, large and small, inhabit Southeast Asian countries. In Thailand alone, there have been recent reports of new species in the genus Macrobrachium. Morphological differentiation and, recently, DNA sequence differences have been used to distinguish new species from valid ones. Macrobrachium chainatense (Decapoda: Palaemonidae), a small freshwater prawn found in Thailand, is now established as a new species based on both morphological and genetic criteria. Among Thailand Macrobrachium prawns, M. chainatense has smallest body size, largest egg size, and 3–5 teeth on the cutting edges. The most closely related Macrobrachium species to M. chainatense in terms of COI and 18S DNA sequences is M. niphanae. Their COI sequences differ by 7.3% while their 18S sequences are basically identical in all but one site. Observations of some aspects of this prawn in captivity are also utilized to establish its unique species status. This prawn may be useful as a source of cooked food for human consumption.
References
Cai, Y. & Ng, P.K.L. (2001) The freshwater Decapod Crustaceans of Halmahera, Indonesia. Journal of Crustacean Biology, 21, 665–695.
https://doi.org/10.1163/20021975-99990167
Cai, Y. & Ng, P.K.L. (2002) The freshwater palaemonid prawns (Crustacea: Decapoda: Caridea) of Myanmar. Hydrobiologia, 487, 59–83.
https://doi.org/10.1023/A:1022991224381
Cai, Y., Naiyanetr, P. & Ng, P.K.L. (2004) The freshwater prawns of the genus Macrobrachium Bate, 1868 of Thailand (Crustacea: Decapoda: Palaemonidae). Journal of Natural History, 38, 581–649.
https://doi.org/10.1080/0022293021000033238
Cai, Y. & Vidthayanon, C. (2016) Macrobrachium spelaeus, a new species of stygobitic freshwater prawn from Thailand (Decapoda: Palaemonidae). Raffles Bulletin of Zoology, 64, 117–122.
Dana, J.D. (1852) Conspectus crustaceorum, &c. Conspectus of the Crustacea of the exploring expedition under Capt. C. Wilkes, U. S. N. Proceedings of the Academy of Natural Science of Philadelphia, 6, 10–28. [species description in Latin]
Dang, N.T. & Nguyen, B.Y. (1972) Nouveaux genres, nouvelles espèces de la faune des invertébres des eaux douces et saumâtres du Nord Vietnam. Tap San Sinh Vat-Dia Hoc [Journal of Biology and Geology], 6, 155–162. [in Vietnamese]
De Grave, S. & Ashelby, C.W. (2013) A re-appraisal of the systematic status of selected genera in Palaemoninae (Crustacea: Decapoda: Palaemonidae). Zootaxa, 3734 (3), 331–344.
https://doi.org/10.11646/zootaxa.3734.3.3
De Man, J.G. (1879) On some species of the genus Palaemon Fabr. with descriptions of two new forms. Notes from the Leyden Museum, 41, 165–184.
De Man, J.G. (1898) Zoological results of the Dutch scientific expedition to Central Borneo. The crustaceans. Part I. Macroura. Notes from the Leyden Museum, 20, 137–161.
De Man, J.G. (1911) On the West-African species of the subgenus Eupalaemon Ortm. Notes from the Leyden Museum, 33, 261–264.
Hanamura, Y., Imai, H., Lasasimma, O., Souliyamath, P. & Ito, S. (2011) Freshwater prawns of the genus Macrobrachium Bate, 1868 (Crustacea: Decapoda: Palaemonidae) from Laos. Zootaxa, 3025 (1), 1–37.
https://doi.org/10.11646/zootaxa.3025.1.1
Holthuis, L.B. (1950) The Decapoda of the Siboga expedition Part X: Subfamily Palaemoninae. The Palaemonidae collected by the Siboga and Snellius Expeditions, with remarks on other species I. Siboga-Expeditie, 39a, 1–268.
Huelsenbeck, J.P. & Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics, 17, 754–755.https://doi.org/10.1093/bioinformatics/17.8.754
Jimoh, A.A., Anetekhai, M.A., Cummings, S., Abanikanda, O.T.F., Turner, G.F., van Oosterhout, C. & Hänfling, B. (2013) Mismatch between molecular (mtDNA) and morphological classification of Macrobrachium prawns from Southern Nigeria: Cryptic freshwater species and brackish water morphotypes. Aquaculture, 410–411, pls. 25–31.
https://doi.org/10.1016/j.aquaculture.2013.06.013
Kingsley, J.S. (1879) List of the North American Crustacea belonging to the suborder Caridea. Bulletin of the Essex Institute, 10, 53–71.
Kowasupat, C., Panijpan, B., Laosinchai, P., Ruenwongsa, P., Phongdara, A., Wanna, W., Senapin, S. & Phiwsaiya, K. (2014) Biodiversity of the Betta smaragdina (Teleostei: Perciformes) in the northeast region of Thailand as determined by mitochondrial COI and nuclear ITS1 gene sequences. Meta Gene, 2, 83–95.
https://doi.org/10.1016/j.mgene.2013.12.004
Mar, W., Kang, P.F., Mao, B. & Wang, Y.F. (2018) Morphological and molecular features of some freshwater prawn species under genus Macrobrachium Spence Bate, 1868 (Crustacea: Decapoda: Palaemonidae) from Myanmar. Zootaxa, 4388 (1), 123–132.
https://doi.org/10.11646/zootaxa.4388.1.9
Medlin, L., Elwood, H.J., Stickel, S. & Sogin, M.L. (1988) The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Gene, 71, 491–499.
https://doi.org/10.1016/0378-1119(88)90066-2
Ng, P.K.L. (1994) On a collection of freshwater Decapod Crustaceans from the Kinabatangan river, Sabah, Malaysia, with descriptions of three new species. Sabah Museum Journal, 1 (2), 73–92.
Ng, P.K.L. (1995) Freshwater Decapod Crustaceans (Potamidae, Palemonidae) of Temengor Forest Reserve, Hulu Perak, Malaysia. Malayan Nature Journal, 48, 249–257.
Ou, A.C.T. & Yeo, D.C.J. (1995) A new species of freshwater prawn, Macrobrachium platycheles (Decapoda: Caridea: Palemondidae) from Singapore and peninsular Malaysia. Raffles Bulletin of Zoology, 43, 299–308.
Page, T.J. & Hughes, J.M. (2011) Neither molecular nor morphological data have all the answers; with an example from Macrobrachium (Decapoda: Palaemonidae) from Australia. Zootaxa, 2874 (1), 65–68.
https://doi.org/10.11646/zootaxa.2874.1.4
Panijpan, B., Kowasupat, C., Laosinchai, P., Ruenwongsa, P., Phongdara, A., Senapin, S., Wanna, W., Phiwsaiya, K., Kühne, J. & Fasquel, F. (2014) Southeast Asian mouth-brooding Betta fighting fish (Teleostei: Perciformes) species and their phylogenetic relationships based on mitochondrial COI and nuclear ITS1 DNA sequences and analyses. Meta Gene, 2, 862–879.https://doi.org/10.1016/j.mgene.2014.10.007
Rafinesque, C.S. (1815) Analyse de la Nature ou Tableau de l’Univers et des Corps Organisés. Author, Palermo, 224 pp. [in French]
https://doi.org/10.5962/bhl.title.106607
Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574.
https://doi.org/10.1093/bioinformatics/btg180
Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61, 539–542.
https://doi.org/10.1093/sysbio/sys029
Saengphan, N., Panijpan, B., Senapin, S., Laosinchai, P., Ruenwongsa, P., Suksomnit, A. & Phiwsaiya, K. (2018) Morphology and molecular phylogeny of Macrobrachium suphanense sp. nov. (Decapoda: Palaemonidae) from Thailand. Zootaxa, 4482 (1), 151–163.
https://doi.org/10.11646/zootaxa.4482.1.7
Shokita, S. & Takeda, M. (1989) A new freshwater prawn of the genus Macrobrachium (Decapoda: Caridea: Palaemonidae) from Thailand. Bulletin of the National Science Museum, Tokyo, 15, 147–154.
Spence Bate, C. (1868) On a new genus, with four new species, of freshwater prawns. Proceedings of the Zoological Society of London, 1868, 363–368.
Wang, H., Qi, M. & Cutler, A.J. (1993) A simple method of preparing plant samples for PCR. Nucleic Acids Research, 21, 4153–4154.
https://doi.org/10.1093/nar/21.17.4153
Wowor, D., Muthu, V., Meier, R., Balke, M., Cai, Y. & Ng, P.K.L. (2009) Evolution of life history traits in Asian freshwater prawns of the genus Macrobrachium (Crustacea: Decapoda: Palaemonidae) based on multilocus molecular phylogenetic analysis. Molecular Phylogenetics and Evolution, 52, 340–350.
https://doi.org/10.1016/j.ympev.2009.01.002
Xie, W., Lewis, P.O., Fan, Y., Kuo, L. & Chen, M.H. (2011) Improving marginal likelihood estimation for Bayesian phylogenetic model selection. Systematic Biology, 60, 150–160.
https://doi.org/10.1093/sysbio/syq085
Xuan, N.V. (2006) A new species of freshwater prawn of the genus Macrobrachium, similar to Macrobrachium sintangense (De Man, 1898) (Decapoda, Caridea, Palaemonidae) from South Vietnam, and description of its first zoea. Crustaceana, 79, 235–254.
https://doi.org/10.1163/156854006776952865
Xuan, N.V. (2012) Macrobrachium hungi, a new freshwater palaemonid prawn (Decapoda: Caridea: Palaemonidae) from the Tonle Sap Great Lake of Cambodia. Zootaxa, 3560 (1), 32–40.
https://doi.org/10.11646/zootaxa.3560.1.2