Abstract
Steinernema feltiae, strain British Columbia (BC) was collected from Vancouver, Canada. SEM, morphometrical and molecular studies were conducted and the results reported. This article presents the SEM study of different stages of S. feltiae. The significant characteristic found in this study is that the formula of the lateral field of S. feltiae is 2, 7, 8, 7, 4, 2. Morphometric comparison of the new isolate and the topotype population (strain RU from Russia) showed that the body length, distance from anterior end to excretory pore, tail length, and hyaline length are significantly different. Molecular studies of rDNA ITS regions showed that the strain BC is closely related to the topotype of S. feltiae (strain RU) from Russia. Sequence length of strain RU is 980 base pairs compared to 977 base pairs for strain BC. Intraspecific relationships among strains of S. feltiae showed that geographic distribution is associated with the genetic differences, but the differences are not sufficient to delimit new species.References
Bovien, P. (1937) Some types of association between nematodes and insects. Videnskabelige Meddelelser Fra Dansk Naturhistorisk Forening Bd., 101, 1–114.
Courtney, W.D., Polley, D. & Miller, V.I. (1955) TAF an improved fixative in nematode technique. Plant Disease Reporter, 39, 570–571.
Filipjev, I.N. (1934) Miscellania Nematologica 1. Eine neueart der Gatung Neoaplectana Steiner nebst Bermerkungen uber die systematische Stellung der letzteren. Magasin de parasitologie de l’Institut Zoologique de l’Academie de l’USSR, 4, 229–240.
Haukeland, S. (1993) Scanning electron micrographs of the anterior region of nematodes in the genus Steinernema (Steinernematidae: Rhabditida). Fundamental and Applied Nematology, 16, 57–61.
Hominick, W.M. (2002) Biogeography. In: Gaugler, R. (Ed.), Entomopathogenic Nematology. New York, NY, USA, CABI Publishing, pp. 115–143.
Maddison, W.P. & Maddison, D.R. (2002) MacClade version 4.0. Sinauer, Massachusetts, Sunderland.
Mr<Öek, Z. (1980) The use of “Galleria traps” for obtaining nematode parasites of insects in Czechoslovakia (Nematoda, Steinernematidae). Acta Entomologica Bohemoslovalovaca, 77, 378–382.
Mr<Öek, Z. & Bednarek, A. (1991) The morphology of lateral fields of infective juveniles of entomopathogenic nematodes of the family Steinernematidae (Rhabditida). Nematologica, 37, 63–71.
Mr<Öek, Z. & Webster, J. (1993) Survey of Heterorhabditidae and Steinernematidae (Rhabditida, Nematoda) in Western Canada. Journal of Nematology, 25, 710–717.
Mr<Öek, Z., Gut, J. & Gerdin, S. (1982) Neoaplectana bibionis Bovien, 1937, an obligate parasite insect isolated from forest soil in Czechoslovakia. Folia Parasitollogica, 29, 139–145.
Mr<Öek, Z., Kindlmann, P. & Webster, J.M. (2005) Steinernema affine (Nematoda: Steinernematidae), a new record for North America and its distribution relative to other entomopathogenic nematodes in British Columbia. Nematology , 7, 495–501.
Nguyen, K.B. & Duncan, L.W. (2002) Steinernema diaprepesi n. sp. (Rhabditida: Steinernematidae), a parasite of the citrus root weevil Diaprepes abbreviatus (L) (Coleoptera : Curculionidae). Journal of Nematology, 34, 159–170.
Nguyen, K.B. & Adams, B.J. (2003) SEM and systematic studies of Steinernema abbasi Elawad et al., 1997 and S. riobrave Cabanillas et al., 1994 (Rhabditida: Steinernematidae). Zootaxa, 179, 1–10.
Nguyen, K.B., Maruniak, J. & Adams, B.J. (2001) The diagnostic and phylogenetic utility of the rDNA internal transcribed spacer sequences of Steinernema. Journal of Nematology, 33, 73–82.
Nguyen, K.B. & Smart, Jr, G.C. (1990) Steinernema scapterisci n. sp. (Steinernematidae: Nematoda). Journal of Nematology, 22, 187–199
Nguyen, K.B. & Smart, Jr., G.C. (1995) Scanning electron microscope studies of Steinernema glaseri (Nematoda: Steinernematidae). Nematologica, 41, 183–190.
Poinar, Jr., G.O. (1989) Examination of the neoaplectanid species feltiae Filipjev, carpocapsae Weiser and bibionis Bovien (Nematoda: Rhabditida). Revue Nematologie, 12, 375–377.
Reid, A.P. & Hominick, W.M. (1992) Restriction fragment length polymorphisms within the ribosomal DNA repeat unit of British entomopathogenic nematodes (Rhabditida: Steinernematidae). Parasitology, 105, 317–323.
Reid, A.P. & Hominick, W.M. (1993) Cloning of the rDNA repeat unit from a British entomopathogenic nematode (Steinernematidae) and its potential or species identification. Parasitology, 107, 529–536.
Seinhorst, J.W. (1959) A rapid method for the transfer of nematodes fromfixative to anhydrous glycerin. Nematologica, 4, 67–69.
Spiridonov, S.E., Reid, A.P., Prodrucka, K., Subbotin, S.A. & Moens, M. (2004) Phylogenetic relationship within the genus Steinernema (Nematoda: Rhabditida) as inferred from analyses of the ITS1-5.8S-ITS2 region of rDNA and morphological features. Nematology, 6, 547–566.
Stock, S.P., Campbell, J.F. & Nadler, S.A. (2001) Phylogeny of Steinernema Travassos, 1927 (Cephalobina: Steinernematidae) inferred from ribosomal DNA sequences and morphological characters. Journal of Parasitology, 87, 877–889.
Swofford, D.L. (2002) PAUP* Phylogenetic Analysis Using Parsimony (*and Other Methods). Sunderland, Massachusetts, Sinauer Associates.
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25, 4876–4882.
Wouts, W.M. (1980) Biology, life cycle and redescription of Neoaplectana bibionis Bovien, 1937 (Nematoda: Steinernematidae). Journal of Nematology, 12, 62–72.
Wouts, W.M., Mr<Öek, Z., Gerdin, S. & Bedding, R.A. (1982) Neoaplectana Steiner, 1929 a junior synonym of Steinernema Travassos, 1927 (Nematoda: Rhabditida). Systematic Parasitology, 4, 147–154.
Yoshida, M. (2003) Intraspecific variation in RFLP patterns and morphological studies on Steinernema feltiae and S. kraussei (Rhabditida: Steinernematidae) from Hokkaido, Japan. Nematology, 5, 735–746.