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Type: Article
Published: 2007-07-26
Page range: 51–68
Abstract views: 114
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Get an eyeful of this: a new species of giant spitting cobra from eastern and north-eastern Africa (Squamata: Serpentes: Elapidae: Naja)

School of Biological Sciences, University of Wales, Bangor LL57 2UW, UK. Tel. +44 1248 382301; Fax: +44 1248 371644
Biodiversity Foundation for Africa, P.O. Box FM 730, Bulawayo, Zimbabwe
Squamata Naja ashei sp. nov. Naja nigricollis Naja mossambica Serpentes Elapidae Africa mitochondrial DNA phylogeny multivariate morphometrics

Abstract

We describe a new species of giant spitting cobra, Naja ashei sp. nov., from eastern and north-eastern Africa. The species was previously regarded as a colour phase of the black-necked spitting cobra, N. nigricollis. However, mtDNA sequence data show it to be more closely related to N. mossambica than N. nigricollis. The new species is diagnosable from all other African spitting cobras by the possession of a unique clade of mtDNA haplotypes and a combination of colour pattern and scalation characteristics. Its distribution includes the dry lowlands of northern and eastern Kenya, north-eastern Uganda, southern Ethiopia and southern Somalia.

References

  1. Angel, F. (1922) Sur une collection de reptiles et de batraciens, recueillis au Soudan français par la mission du Dr. Millet Horsin. Bulletin du Muséum national d’Histoire naturelle de Paris 28, 39–41.

    Arévalo, E., Davis, S.K. & Sites, J.W. (1994) Mitochondrial DNA sequence divergence and phylogenetic relationships among eight chromosome races of the Sceloporus grammicus complex (Phrynosomatidae) in Central Mexico. Systematic Biology, 43, 387–418.

    Bocage, J. V. Barboza du (1895) Herpétologie d’Angola et du Congo. Imprimerie Nationale, Lisbon, 203 pp.

    Bogert, C.M. (1940) Herpetological results of the Vernay-Angola Expedition, with notes on African reptiles in other collections. I. Snakes, including an arrangement of African Colubridae. Bulletin of the American Museum of Natural History, 77, 1–107.

    Bogert, C.M. (1943) Dentitional phenomena in cobras and other elapids, with notes on adaptive modification of fangs. Bulletin of the American Museum of Natural History, 81, 285–360.

    Boulenger, G.A. (1896) Catalogue of the Snakes in the British Museum (Natural History) 3. British Museum (Natural History), London, 727 pp.

    Boycott, R.C. & Haacke, W.D. (1979) Note on the type-locality, distribution and juvenile colouration of Naja nigricollis woodi (Serpentes: Elapidae) and an account of the colour- pattern variation in intergrade populations. Annals of the Cape Provincial Museums (Natural History), 13, 31–38.

    Branch, W. R. (1979) The venomous snakes of southern Africa Part 2. Elapidae and Hydrophidae. Snake, 11, 199-225.

    Broadley, D.G. (1968) A review of the African cobras of the genus Naja (Serpentes: Elapinae). Arnoldia, 3, 1–14.

    Broadley, D.G. (1974) A review of the cobras of the Naja nigricollis complex in south‑western Africa. Cimbebasia (A), 2,155–162.

    Broadley, D.G. & Wüster W. (2004) A review of the southern African ‘non-spitting’ cobras (Serpentes: Elapidae: Naja). African Journal of Herpetology, 53,101–122.

    Burbrink, F.T., Lawson, R. & Slowinski, J.B. (2000) Mitochondrial DNA phylogeography of the polytypic North American Rat Snake (Elaphe obsoleta): a critique of the subspecies concept. Evolution, 54, 2107–2118.

    Castoe, T.C., Doan, T.M. & Parkinson, C.L. (2004) Data partitions and complex models in Bayesian analysis: the phylogeny of gymnophthalmid lizards. Systematic Biology, 53, 448–469.

    Castoe, T.A. & Parkinson, C.L. (2006) Bayesian mixed models and the phylogeny of pitvipers (Viperidae: Serpentes) Molecular Phylogenetics and Evolution, 39, 99–110.

    Castoe, T.C., Sasa, M. & Parkinson, C.L. (2005) Modeling nucleotide evolution at the mesoscale: The phylogeny of the Neotropical pitvipers of the Porthidium group (Viperidae: Crotalinae). Molecular Phylogenetics and Evolution, 37, 881–898.

    Felsenstein, J. (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39, 783–791.

    Fry, B.G., Winkel, K.D., Wickramaratna, J.C., Hodgson, W.C. & Wüster, W. (2003) Effectiveness of snake antivenom: species and regional venom variation and its clinical impact. Journal of Toxicology -Toxin Reviews, 22, 23–34.

    Good, D.A. (1994) Species limits in the genus Gerrhonotus (Squamata: Anguidae). Herpetological Monographs, 7, 180–202.

    Günther, A. (1894) Second report on the reptiles and batrachians transmitted by Mr. H.H. Johnston, C.B., from British Central Africa. Proceedings of the Zoological Society of London, 1893, 616–628.

    Hillis, D.M. & Huelsenbeck, J.P. (1992) Signal, noise and reliability in phylogenetic analyses. Journal of Heredity, 83, 189–195.

    Hughes, B. (1983) African snake faunas. Bonner Zoologische Beiträge, 34, 311-356.

    Hughes, B. & Barry, D. H. (1969) The snakes of Ghana. Bulletin de l’Institut fondamental d’Afrique noire, A31, 1004‑1041.

    Kumar, S., Tamura, K., Jakobsen, I.B. & Nei., M. (2001) MEGA2: molecular evolutionary genetics analysis software. Bioinformatics ,17, 1244–1245

    Laurent, R.F. (1955) Diagnoses préliminaires de quelques serpents venimeux. Revue de Zoologie et Botanique Africaine, 51, 127–139.

    Laurent, R.F. (1956) Contribution à l’herépetologie de la région des Grands Lacs de l’Afrique centrale. I. Généralitiés. II. Chéloniens. III. Ophidiens. Annales du Musée royal du Congo Belge, Série in 8o, 48, 1–390.

    Laurent, R.F. (1964) Reptiles et Amphibiens de l’Angola. (Troisième contribution). Publicações Culturais da Comphania de Diamantes de Angola, 67, 11–165.

    Laurent, R.F. (1973) Le concept de sous-espèce et la variation géographique chez Naja melanoleuca et Naja nigricollis. Revue de Zoologie et Botanique Africaine, 87, 198–204.

    Leviton, A.E., Gibbs, R.H. Jr., Heal, E. & Dawson, C.E. (1985) Standards in herpetology and ichthyology: Part I. Standard symbolic codes for institutional resource collections in herpetology and ichthyology. Copeia 1985, 802-832.

    Leviton, A.E. & Gibbs, R.H. Jr. (1988). Standards in herpetology and ichthyology: Part I. Standard symbolic codes for institutional resource collections in herpetology and ichthyology. Supplement No. 1: Additions and corrections. Copeia 1988, 280-282.

    Mirtschin, P.J., Dunstan, N., Hough, B., Hamilton, E., Klein, S., Lucas, J., Millar, D., Madaras, F.& Nias, T. (2006) Venom yields from Australian and some other species of snakes. Ecotoxicology, 15, 531–538

    Nylander, J.A.A. (2004) MrModeltest v2. Program distributed by the author. Evolutionary Biology Centre, Uppsala University, Uppsala.

    Ogden, R. & Thorpe, R.S. (2002) Molecular evidence for ecological speciation in tropical habitats. Proceedings of the National Academy of Sciences, 99, 13612–13615.

    Palumbi, S.R. (1996) Nucleic acids II: the polymerase chain reaction. In: Hillis, D.M., Moritz, C. & Mable, B.K. (Eds.), Molecular Systematics. Second Edition. Sinauer, Sunderland, Massachusetts, pp. 205–247.

    Peters, W.C.H. (1854) Diagnosen neuer Batrachier, welche zusammen mit der früher (24. Juli und 17. August) gegebenen Übersicht der Schlangen und Eidechsen mitgetheilt werden. Bericht über zur Bekanntmachung geeignete Verhandlungen der Königlichen preussischen Akademie der Wissenschaften zu Berlin, 1854, 614–628.

    Pitman, C.R.S. (1974). A Guide to the Snakes of Uganda. Revised Edition. Wheldon & Wesley, Codicote, 290 pp.

    Pringle, J.A. (1955) A new subspecies of the spitting cobra Naja nigricollis Reinhardt from the Cape Province. Annals of the Natal Museum, 13, 253–254.

    Puorto, G., Salomão, M.G., Theakston, R.D.G., Thorpe, R.S., Warrell, D.A. & Wüster, W. (2001) Combining mitochondrial DNA sequences and morphological data to infer species boundaries: phylogeography of lanceheaded pitvipers in the Brazilian Atlantic forest, and the status of Bothrops pradoi (Squamata: Serpentes: Viperidae). Journal of Evolutionary Biology, 14, 527–538

    Reinhardt, J.T. (1843) Beskrivelse af nogle nye Slangearter. Det Kongelige Danske Videnskabernes Selskabs Naturvidenskabelige og Mathematiske Afhandlinger, 10, 233–277.

    Roman, B. (1968) Une nouvelle espèce de Naja en Haute-Volta. Carrefour Africain 299: 273–274, 286, 310.

    Roman, B. (1969) Les Naja de Haute-Volta. Revue de Zoologie et Botanique Africaine, 82, 1–14.

    Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574.

    Sambrook, F., Fritsch, E.F. & Maniatis, T (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.

    Slowinski, J.B. & Wüster, W. (2000) A new cobra (Elapidae: Naja) from Myanmar (Burma). Herpetologica, 56, 257–270.

    Smith, A. (1838) Contributions to South African Zoology. Magazine of Natural History, 2, 30–33, 92–94.

    Smith, A. (1842) Illustrations of the Zoology of South Africa—Reptilia. London: Smith. Elder & Co., Pls. i–ixxviii. Part 16 (Plate x).

    Spawls, S. (2004) James Ashe: 1925–2004. African Journal of Herpetology, 53, 203–204.

    Spawls, S. & Branch, B. (1995) The Dangerous Snakes of Africa. Blandford, London, UK, 192 pp..

    Spawls S., Howell K., Drewes R. & Ashe J. (2002) A field guide to the reptiles of East Africa. Academic Press, London, 543 pp.

    Swofford, D.L. (2002) PAUP*—Phylogenetic Analysis Using Parsimony (*and Other Methods). Beta version 4.0b10. Sinauer, Sunderland.

    Templeton, A.R. (1983) Phylogenetic inference from restriction endonuclease cleavage sites maps with particular reference to the evolution of humans and the apes. Evolution, 37, 221–244.

    Thorpe, R.S. & Richard, M. (2001) Evidence that ultraviolet markings are associated with patterns of molecular gene flow. Proceedings of the National Academy of Sciences, 98, 3929–3934.

    Thorpe, R.S., Brown, R.P., Day, M., Malhotra, A., McGregor, D.P. & Wüster, W. (1994) Testing ecological and phylogenetic hypotheses in microevolutionary studies: an overview. In: Eggleton, P. & Vane-Wright, R. (Eds.), Phylogenetics and Ecology. Academic Press, London, pp. 189–206.

    Thorpe, R.S., Malhotra, A. Black, H., Daltry, J.C. & Wüster, W. (1995) Relating geographic pattern to phylogenetic process. Philosophical Transactions of the Royal Society of London, B., 349, 61–68.

    Tilbury, C.R. (1982) Observations on the bite of the Mozambique spitting cobra (Naja mossambica mossambica). South African Medical Journal, 61, 308–313.

    Warrell, D.A., B.M. Greenwood, N. McD. Davidson, L.D. Ormerod & C.R.M. Prentice (1976) Necrosis, haemorrhage and complement depletion following bites by the spitting cobra (Naja nigricollis). Quarterly Journal of Medicine, New Series, 45, 1–22.

    Wüster, W. (1996) Taxonomic changes and toxinology: systematic revisions of the Asiatic cobras (Naja naja species complex). Toxicon, 34, 399–406.

    Wüster, W. (1998) The cobras of the genus Naja in India. Hamadryad, 23, 15–32.

    Wüster, W. & Broadley, D.G. (2003) A new species of spitting cobra from north-eastern Africa (Serpentes: Elapidae: Naja). Journal of Zoology, London, 259, 345–359.

    Wüster, W. & McCarthy, C.J. (1996) Venomous snake systematics: implications for snakebite treatment and toxinology. In C. Bon & M. Goyffon (Eds.), Envenomings and their Treatments. Fondation Mérieux, Lyon, pp. 13–23.

    Wüster, W. & Thorpe, R.S. (1989) Population affinities of the Asiatic cobra (Naja naja) species complex in south‑east Asia: reliability and random resampling. Biological Journal of the Linnean Society, 36, 391–409.

    Wüster, W. & Thorpe, R.S. (1991) Asiatic cobras: systematics and snakebite. Experientia, 47, 205–209.

    Wüster, W. & Thorpe, R.S. (1992) Asiatic cobras: population systematics of the Naja naja species complex (Serpentes: Elapidae) in India and Central Asia. Herpetologica, 48, 69–85.

    Wüster, W., Thorpe, R.S., Cox, M.J., Jintakune, P., Nabhitabhata, J. (1995) Population systematics of the snake genus Naja (Reptilia: Serpentes: Elapidae) in Indochina: multivariate morphometrics and comparative mitochondrial DNA sequencing (cytochrome oxidase I). Journal of Evolutionary Biology, 8, 493–510.

    Wüster, W., Warrell, D.A., Cox, M.J. Jintakune, P., Nabhitabhata, J. (1997) Redescription of Naja siamensis Laurenti, 1768 (Serpentes: Elapidae), a widely overlooked spitting cobra from Southeast Asia: geographic variation, medical importance and designation of a neotype. Journal of Zoology, London, 243, 771–788.

    Zhang, D.-X. & Hewitt, G.M. (1996) Nuclear integrations: challenges for mitochondrial DNA markers. Trends in Ecology and Evolution, 11, 247–251.