Abstract
Paraschistura makranensis, new species, is described from the Jegin River drainage in southern Iran. It is distinguished from its congeners in Iran by having a plain brown or slightly mottled colour pattern on the flanks, a very slender body with a relatively short head, a complete lateral line extending almost to the caudal-fin base, the caudal peduncle covered by scales, and a suborbital flap in males. Paraschistura makranensis is distinguished from P. bampurensis and P. hormuzensis, its closest relatives in our dataset, by an uncorrected-pairwise distance based on the COI data of 4.0 and 5.4%, respectively. We treat Paraschistura pasatigris as a synonym of P. ilamensis.
References
Altschul, S.F., Gish, W., Miller, W., Myers, E.W. & Lipman, D.J. (1990) Basic local alignment search tool. Journal of Molecular Biology, 215 (3), 403–410.
https://doi.org/10.1016/S0022-2836(05)80360-2
Azimi, H., Mousavi-Sabet, H. & Eagderi, S. (2015a) Osteological characteristics of Paraschistura nielseni (Cypriniformes, Nemacheilidae). Iranian Journal of Ichthyology, 2 (3), 155–164.
Azimi, H., Mousavi-Sabet, H., Eagderi, S. & Vatandoust, S. (2015b) Osteological characteristics of Turkmenian stone loach, Paraschistura cristata (Cypriniformes: Nemacheilidae). International Journal of Aquatic Biology, 3 (5), 290–300.
Freyhof, J., Esmaeili, H.R., Sayyadzadeh, G. & Geiger, M. (2014) Review of the crested loaches of the genus Paracobitis from Iran and Iraq with the description of four new species (Teleostei: Nemacheilidae). Ichthyological Exploration of Freshwaters, 25, 11–38.
Freyhof, J., Sayyadzadeh, G., Esmaeili, H.R. & Geiger, M. (2015) Review of the genus Paraschistura from Iran with description of six new species (Teleostei: Nemacheilidae). Ichthyological Exploration of Freshwaters, 26 (1), 1–48.
ICZN (International Code of Zoological Nomenclature) (1999) International Code of Zoological Nomenclature. 4th Edition. International Trust for Zoological Nomeclature, London, 306 pp.
Jouladeh-Roudbar, A., Eagderi, S. & Vatandoust, S. (2015a) First record of Paraschistura alta (Nalbant and Bianco, 1998) from Eastern Iran and providing its COI barcode region sequences (Teleostei: Nemacheilidae). Iranian Journal of Ichthyology, 2 (4), 235–243.
Jouladeh-Roudbar, A., Vatandoust, S., Eagderi, S., Jafari-Kenari, S. & Mousavi-Sabet, H. (2015b) Freshwater fishes of Iran; an updated checklist. AACL Bioflux, 2438 (6), 855–909.
Kalyaanamoorthy, S., Minh, B.Q., Wong, T.K.F., von Haeseler, V. & Jermiin, L.S. (2017) ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods, 14, 587–589.
https://doi.org/10.1038/nmeth.4285
Kottelat, M. & Freyhof, J. (2007) Handbook of European freshwater fishes. Publications Kottelat, 646 pp.
Kumar, S., Nei, M, Dudldey, J. & Tamura, K. (2008) MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Briefings in Bioinformatics, 9, 299–306.
https://doi.org/10.1093/bib/bbn017
Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin, F., Wallace, I.M., Wilm, A., Lopez, R., Thompson, J.D., Gibson, T.J. & Higgins, D.G. (2007) Clustal W and Clustal X version 2.0. Bioinformatics, 23 (21), 2947–2948.
https://doi.org/10.1093/bioinformatics/btm404
Mousavi-Sabet, H. & Eagderi, S. (2015) Paraschistura delvarii spec. nov.—a new species of stone loach from the Persian Gulf basin, southern Iran (Teleostei: Nemacheilidae). Vertebrate Zoology, 65 (3), 297–303.
Mousavi-Sabet, H., Vatandoust, S., Geiger, M.F. & Freyhof, J. (2019) Paracobitis abrishamchiani, a new crested loach from the southern Caspian Sea basin (Teleostei: Nemacheilidae). Zootaxa, 4545 (3), 375–388.
https://doi.org/10.11646/zootaxa.4545.3.3
Mousavi-Sabet, H., Vatandoust, S., Jouladeh Roudbar, A. & Eagderi, S. (2015) Taxonomic status of the genus Paraschistura (Teleostei: Nemacheilidae) in the Hari river basin, with re-validation of P. turcomana. Journal of Applied Biological Sciences, 9 (3), 1–5.
Nei, M. & Kumar, S. (2000) Molecular Evolution and Phylogenetics. Oxford University Press, New York, 333 pp.
Nguyen, L.-T., Schmidt, H.A., von Haeseler, A., & Minh B.Q. (2015) IQ-TREE: A fast and effective stochastic algorithm for estimating maximum likelihood phylogenies. Molecular Biology and Evolution, 32, 268–274.
https://doi.org/10.1093/molbev/msu300
Posada, D. & Crandall, K.A. (2001) Intraspecific gene genealogies: trees grafting into networks. Trends in Ecology and Evolution, 16 (1), 37–45.
https://doi.org/10.1016/S0169-5347(00)02026-7
Rambaut, A. & Drummond, A.J. (2013) Tracer. Version 1.5. Available from: http://beast.bio.ed.ac.uk/Tracer (accessed 10 August 2019)
Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S. & Huelsenbeck, J.P. (2011) MrBayes 3.2: efficient bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61 (3), 539–542.
https://doi.org/10.1093/sysbio/sys029
Sayyadzadeh, G., Teimori, A. & Esmaeili, H.R. (2019) Paraschistura kermanensis, a new stone loach species from Kerman-Naein basin in Iran (Teleostei: Nemacheilidae). Zootaxa, 4638 (4), 571–583.
https://doi.org/10.11646/zootaxa.4638.4.7
Tamura, K., Stecher, G., Peterson, D. Filipski, A. & Kumar, S. (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729.
https://doi.org/10.1093/molbev/mst197
Vatandoust, S. & Eagderi, S. (2015) Paraschistura ilamensis, a new species of loach from the Tigris River drainage (Teleostei: Nemacheilidae). International Journal of Aquatic Biology, 3 (3), 177–182.
Ward, R.D., Zemlak, T.S., Innes, B.H., Last, P.R. & Hebert, P.D. (2005) DNA barcoding Australia’s fish species. Philosophical Transactions of the Royal Society of London B: Biological Sciences, 360 (1462), 1847–1857.
https://doi.org/10.1098/rstb.2005.1716