Abstract
A new species of the Marphysa sanguinea group, M. iloiloensis n. sp. (Annelida: Eunicida: Eunicidae), is described from the Marine Annelids Hatchery of the Southeast Asian Fisheries Development Center, Aquaculture Department (SEAFDEC- AQD), Iloilo Province, Philippines. It represents the first record of this group in the Philippines. The new species is most similar morphologically to M. hongkongensa Wang, Zhang & Qiu, 2018, but can be distinguished from it by having fewer branchial filaments, a pair of faint eyes (absent in M. hongkongensa), and in slight differences in jaw morphology and chaetation. The embryos of the new species develop inside a jelly cocoon attached to the entrance of the adult burrow; this is the first time that egg-containing cocoons have been found in any species of the sanguinea-group. Phylogenetic analysis based on the mitochondrial gene cytochrome c oxidase subunit I (COI) revealed that Marphysa iloiloensis n. sp. is genetically distinct from all other analysed Marphysa species and forms a sister group to M. hongkongensa. A revised identification key to members of the sanguinea-group in Southeast Asia is provided.
References
Anonymous (2017) Queensland marine worm identification guide. Department of Agriculture and Fisheries, Queensland, 15 pp. Available from: http://www.daf.qld.gov.au (Accessed 23 Sept. 2019)
Alava, V.R., Biñas, J.B. & Mandario, M.A.E. (2017) Breeding and culture of the polychaete, Marphysa mossambica, as feed for the mud crab, Scylla serrata broodstock. In: Quinitio, E. T., Parado-Estepa, F.D. & Coloso, R.M. (Eds.), Philippines: In the forefront of the mud crab industry: Proceedings of the 1st National Mud Crab Congress. Aquaculture Department, Southeast Asian Fisheries Development Center, Tigbauan, Iloilo, pp. 39–45.
Altschul, S.F., Gish, W., Miller, W., Myers, E.W. & Lipman, D.J. (1990) Basic local alignment search tool. Journal of Molecular Biology, 215, 403–410.
https://doi.org/10.1016/S0022-2836(05)80360-2
Augener, H. (1918) Polychaeta. Beitrage zur Kenntnis der Meeresfauna Westafrikas, 2 (2), 67–625.
Baird, W. (1869) Remarks on several genera of annelides, belonging to the group Eunicea, with a notice of such species as are contained in the collection of the British Museum, and a description of some others hitherto undescribed. Journal of the Linnean Society of London, 10, 341–361.
https://doi.org/10.1111/j.1096-3642.1869.tb00665.x
Berthold, A.A. (1827) Latreille’s Naturliche Familien des Thierreichs. Im Verlage des Gr. H.S. priv. Landes-Industrie-Compositoirs, Weimar, 606 pp.
Colgan, D.J., Hutchings, P.A. & Brown, S. (2001) Phylogenetic relationships within the Terebellomorpha. Journal of the Marine Biological Association of the United Kingdom, 81, 765–773.
https://doi.org/10.1017/S002531540100457X
Crossland, C. (1903) On the marine fauna of Zanzibar and British East Africa, from collections made by Cyril Crossland in the years 1901 and 1902. Polychaeta. Part II. Proceedings of the Zoological Society of London, 2, 129–144.
https://doi.org/10.1111/j.1469-7998.1903.tb08273.x
Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9 (8), 772.
https://doi.org/10.1038/nmeth.2109
Elgetany, A.H., El-Ghobashy, A.E., Ghoneim, A.M. & Struck, T.H. (2018) Description of a new species of the genus Marphysa (Eunicidae), Marphysa aegypti sp. n., based on molecular and morphological evidence. Invertebrate Zoology, 15 (1), 71–84.
Fauchald, K. (1970) Polychaetous annelids of the families Eunicidae, Lumbrineridae, Iphitimidae, Arabellidae, Lysaretidae and Dorvilleidae from western Mexico. Allan Hancock Monograph in Marine Biology, 5, 1−335.
Fauchald, K. (1992) A review of the genus Eunice (Polychaeta: Eunicidae) based upon type material. Smithsonian Contributions to Zoology, 523, 1−422.
https://doi.org/10.5479/si.00810282.523
Glasby, C.J. & Hutchings, P.A. (2010) A new species of Marphysa Quatrefages, 1865 (Polychaeta: Eunicida: Eunicidae) from northern Australia and a review of similar taxa from the Indo-west Pacific, including the genus Nauphanta Kinberg, 1865. Zootaxa, 352 (1), 29–45.
https://doi.org/10.11646/zootaxa.2352.1.2
Gravier, C. (1907) Sur les annélides polychètes rapportés par M. le Dr Rivet, de Payta (Pérou). Bulletin du Muséum d’Histoire Naturelle, Paris, 13 (7), 525–530.
Grube, A.E. (1878) Annulata Semperiana. Beiträge zur kenntnis der annelidenfauna der Philippinen nach den von Herrn Prof. Semper mitgebrachten sammlungen. Mémoires de l’Académie Impériale des Sciences de St. Petersbourg, Série 7, 25 (8), i–ix + 1–30.
https://doi.org/10.5962/bhl.title.85345
Hansen, G.A. (1882) Recherches sur les annélides recueillies par M. le professeur Édouard van Benedon pendant son voyage au Brésil et à la Plata. Memoires Couronnes et Memoires des Savants Etrangers publies par L’Academie Royale des Sciences, des Lettres et des Beaux-Arts de Belgique, 44 (3), 1–29.
Hutchings, P.A. & Karageorgopolous, P. (2003) Designation of a neotype of Marphysa sanguinea (Montagu, 1813) and a description of a new species of Marphysa from eastern Australia. Hydrobiologia, 496, 87–94.
https://doi.org/10.1023/A:1026124310552
Idris, I., Hutchings, P. & Arshad, A. (2014) Description of a new species of Marphysa Quatrefages, 1865 (Polychaeta: Eunicidae) from the west coast of Peninsular Malaysia and comparison with species from Marphysa Group A from the Indo-West Pacific and Indian Ocean. Memoirs of Museum Victoria, 71, 109–121.
https://doi.org/10.24199/j.mmv.2014.71.11
Izuka, A. (1907) On two new species of annelids belonging to the Euncidae. Zoological Magazine (Dobutsugasku zasshi), Tokyo, 19, 139–143.
Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution, 30 (4), 772–780.
https://doi.org/10.1093/molbev/mst010
Kinberg, J.G.H. (1865) Annulata nova. Öfversigt af Kongliga Vetenskaps-Akademiens Förhandlingar, Stockholm, 21, 559–574.
Kumar S, Stecher, G., Li, M., Knyaz, C. & Tamura, K. (2018) MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution, 35, 1547–1549.
https://doi.org/10.1093/molbev/msy096
Lavesque, N., Daffe, G., Bonifácio, P. & Hutchings, P.A. (2017) A new species of the Marphysa sanguinea complex from French waters (Bay of Biscay, NE Atlantic) (Annelida, Eunicidae). ZooKeys, 716, 1–17.
https://doi.org/10.3897/zookeys.716.14070
Lavesque, N., Daffe, G., Grall, J., Zanol, J., Gouillieux, B. & Hutchings, P.A. (2019) Guess who? On the importance of using appropriate name: case study of Marphysa sanguinea (Montagu, 1803). ZooKeys, 859, 1–15.
https://doi.org/10.3897/zookeys.859.34117
Lewis, C. & Karageorgopoulos, P. (2008) A new species of Marphysa (Eunicidae) from the Western Cape of South Africa. Journal of the Marine Biological Association of the United Kingdom, 88, 277–287.
https://doi.org/10.1017/S002531540800009X
Liu, Y., Hutchings, P.A. & Sun, S. (2017) Three new species of Marphysa Quatrefages, 1865 (Polychaeta: Eunicida: Eunicidae) from the south coast of China and redescription of Marphysa sinensis Monro, 1934. Zootaxa, 4263 (2), 228–250.
https://doi.org/10.11646/zootaxa.4377.2.3
Liu, Y., Hutchings, P.A. & Kupriyanova, E. (2018) Two new species of Marphysa Quatrefages, 1865 (Polychaeta: Eunicida: Eunicidae) from northern coast of China and redescription for Marphysa orientalis Treadwell, 1936. Zootaxa, 4377 (2), 191–215.
https://doi.org/10.11646/zootaxa.4377.2.3
Molina-Acevedo, I.C. (2018) Morphological revision of the Subgroup 1 Fauchald, 1970 of Marphysa de Quatrefages, 1865 (Eunicidae: Polychaeta). Zootaxa, 4480 (1), 1–125.
https://doi.org/10.11646/zootaxa.4480.1.1
Molina-Acevedo, I.C. & Carrera-Parra, L.F. (2015) Reinstatement of three species of the Marphysa sanguinea complex (Polychaeta: Eunicidae) from the Grand Caribbean Region. Zootaxa, 3925 (1), 37–55.
https://doi.org/10.11646/zootaxa.3925.1.3
Molina-Acevedo, I.C. & Carrera-Parra, L.F. (2017) Revision of Marphysa de Quatrefages, 1865 and some species of Nicidion Kinberg, 1865 with the erection of a new genus (Polychaeta: Eunicidae) from the Grand Caribbean. Zootaxa, 4241 (1), 1–62.
https://doi.org/10.11646/zootaxa.4241.1.1
Monro, C.C.A. (1933) The Polychaeta Errantia collected by Dr. C. Crossland at Colón, in the Panama region, and the Galapagos Islands during the Expedition of the S.Y. ‘St. George’. Proceedings of the Zoological Society of London, 103 (1), 1–96.
https://doi.org/10.1111/j.1096-3642.1933.tb01578.x
Montagu, G. (1813) Descriptions of several new or rare animals, principally marine, found on the south coast of Devonshire. Transactions of the Linnean Society of London, 11, 20–21.
https://doi.org/10.1111/j.1096-3642.1813.tb00035.x
Okuda, S. (1934) A new species of errantiate polychaete, Marphysa tamurai n. sp. Proceedings of the Imperial Academy, Tokyo, 10 (8), 421–423.
https://doi.org/10.2183/pjab1912.10.521
Palpal-latoc, V.S. (1981) Inventory of polychaetous annelids from Tayabas Bay. Zoological Papers, National Museum Manila Philippines, 9, 1–55.
Paxton, H. (2000) Family Eunicidae. In: Beesley, P.L., Ross, G.J.B. & Glasby, C.J. (Eds.), Polychaetes & Allies. The Southern Synthesis. Fauna of Australia. Vol 4A. Polychaeta, Myzostomida, Pogonophora, Echiura, Sipuncula. CSIRO Publishing, Melbourne, pp. 94−96.
Peters, W. (1854) Über die Gattung Bdella, Savigny, (Limnatis, Moquin-Tandon) und die in Mossambique beobachteten Anneliden, wovon hier eine Mittheilung folgt [informal title in meeting report]. Bericht über die zur Bekanntmachung geeigneten Verhandlungen der Königlichen Preussischen Akademie der Wissenschaften zu Berlin, 1854, 607–614.
Pillai, T.G. (1958) Studies on a brackish-water polychaetous annelid, Marphysa borradailei sp. n. from Ceylon. Ceylon Journal of Science (Biological Sciences), 1 (2), 94–106.
Pillai, T.G. (1962) Fish farming methods in the Philippines, Indonesia and Hong Kong. FAO Fisheries Biology Technical Paper, 18, 1–68.
Pillai, T.G. (1965) Annelida Polychaeta from the Philippines and Indonesia. Ceylon Journal of Science, 5 (2), 112–177.
Quatrefages, A. de (1865) Histoire naturelle des Annéles marins et d’eau douce. Librairie Encyclopédique de Roret, Paris, 794 pp.
https://doi.org/10.5962/bhl.title.122818
Quatrefages, A. de (1866) (1865) Histoire naturelle des Annelés marins et d’eau douce. Vol. 1. Annélides et Géphyriens. Librarie Encyclopédique de Roret, Paris, 588 pp.
https://doi.org/10.5962/bhl.title.122818
Rambaut A. (2007) FigTree. Available from http://tree.bio.ed.ac.uk/software/figtree/ (accessed 29 March 2019)
Read, G. & Fauchald, K. (2018) World Polychaeta database. Marphysa Quatrefages, 1866. Available from : http://marinespecies.org/aphia.php?p=taxdetails&id=129281 (accessed 30 June 2018)
Ronquist, F. &. Huelsenbeck, J.P. (2003) MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574.
https://doi.org/10.1093/bioinformatics/btg180
Rosito, R.M. (1983) Polychaeta from shallow waters off Mactan, Cebu. Part 2. The Philippine Scientist, 20, 11–38.
Salazar-Vallejo, S.I., Carrera-Parra, L.F., Muir, A.I., de León-González, J.A. Piotrowski, C. & Sato, M. (2014) Polychaete species (Annelida) described from the Philippine and China Seas. Zootaxa, 3842 (1), 1–68.
https://doi.org/10.11646/zootaxa.3842.1.1
Schmarda, L.K. (1861) Neue wirbellose thiere beobachtet und gesammelt auf einer reise un die erdr 1853 bis 1857. Vol. I. Turbellarian, Rotatorien und Anneliden. Wilhelm Engelmann, Leipzig, 164 pp.
Southern, R. (1921) Polychaeta of the Chilka Lake and also of fresh and brackish waters in other parts of India. Memoirs of the Indian Museum Calcutta, 5, 563–659.
Treadwell, A.L. (1917) Polychaetous annelids from Florida, Puerto Rico, Bermuda and the Bahamas. Carnegie Institute of Washington Publication, 251, 255–272.
Treadwell, A.L. (1921) Leodicidae of the West Indian region. Carnegie Institute of Washington Publication, 15 (293), 1–131.
https://doi.org/10.5962/bhl.part.20335
Treadwell, A.L. (1936) Polychaetous annelids from Amoy, China. Proceedings of the United States National Museum, 83, 261−278.
https://doi.org/10.5479/si.00963801.83-2984.261
Treadwell, A.L. (1939) New polychaetous annelids from New England, Texas and Puerto Rico. American Museum Novitates, 1023, 1–7.
Wang, Z., Zhang, Y. & Qiu, J.-W. (2018) A new species of the Marphysa sanguinea complex (Annelida, Eunicidae) from Hong Kong. Zoological Studies, 57, 48.
https://doi.org/10.6620/ZS.2018.57-48
Webster, H.E. (1884) Annelida from Bermuda collected by G. Brown Goode. Bulletin of the United States National Museum, 25, 305–327.
Zanol, J., Halanych, K.M., Struck, T.H. & Fauchald, K. (2010) Phylogeny of the bristle worm family Eunicidae (Eunicida, Annelida) and the phylogenetic utility of noncongruent 16S, COI and 18S in combined analyses. Molecular Phylogenetics and Evolution, 55, 660–676.
https://doi.org/10.1016/j.ympev.2009.12.024
Zanol, J., Halanych, K.M. & Fauchald, K. (2014) Reconciling taxonomy and phylogeny in the bristle worm family Eunicidae (polychaete, Annelida). Zoologica Scripta, 43 (1), 79–100.
https://doi.org/10.1111/zsc.12034
Zanol, J., Silva, T.D.S. & Hutchings, P.A. (2016) Marphysa (Eunicidae, Polychaete, Annelida) species of the Sanguinea group from Australia, with comments on pseudocryptic species. Invertebrate Biology, 135 (4), 328–344.
https://doi.org/10.1111/ivb.12146