Abstract
Species distribution and composition of the ciliates found in the ruminal contents of 15 domestic sheep (Ovis aries) living in Kastamonu, Turkey, were investigated. Twenty-four species and 8 morphotypes belonging to 10 genera were identified. The density of the rumen ciliates in sheep was 108.2 (± 150.8) × 104 cells ml−1, and the mean number of ciliate species per host was 10.7 ± 4.6. Among the ciliate species identified, Dasytricha ruminantium was the most abundant, with a prevalence of 93.3%, whereas Diplodinium quinquespinosum, Eudiplodinium rostratum, and Metadinium tauricum were detected in only one animal (6.7% prevalence). D. quinquespinosum, Eudiplodinium rostratum, and Entodinium simulans m. dubardi were detected for the first time in sheep from Turkey. This is the first report of the presence of D. quinquespinosum in the rumen of ruminants in Turkey. The infraciliature of D. quinquespinosum, Metadinium affine, and M. tauricum (Entodiniomorphida, Ophryoscolecidae) was investigated. D. quinquespinosum and M. affine had Diplodinium-type infraciliature, whereas M. tauricum possessed Metadinium medium-type infraciliature.
References
Baraka, T.A. (2012) Comparative studies of rumen pH, total protozoa count, generic and species composition of ciliates in camel, buffalo, cattle, sheep and goat in Egypt. Journal of American Science, 8 (2), 655–669.
Burritt, E. & Frost, R. (2006) Chapter 2: Animal Behaviour Principles and Practices. In: Launchbaugh, K.L., Walker, J.W. & Daines, R.J. (Eds.), Targeted Grazing: A Natural Approach to Vegetation Management and Landscape Enhancement. American Sheep Industry Association, Englewood, pp. 10–21.
Cannas, A. & Pulina, G. (2008) Dairy Goats Feeding and Nutrition. CAB International, Wallingford, 293 pp.
https://doi.org/10.1079/9781845933487.0000
Cedrola, F., Martinele, I., Dias, R.J.P., Fregulia, P. & D’Agosto, M. (2016) Rumen ciliates in Brazilian sheep (Ovis aries), with new records and redescription of Entodinium contractum (Entodiniomorphida: Ophryoscolecidae). Zootaxa, 4088 (2), 292–300.
http://dx.doi.org/10.11646/zootaxa.4088.2.10
Cedrola, F., Rossi, M.F., Martinele, I., D’Agosto, M. & Dias, R.J.P. (2018) Morphology and description of infraciliary bands pattern in four Metadinium Awerinzew & Mutafowa, 1914 species (Ciliophora, Entodiniomorphida, Ophryoscolecidae) with taxonomic notes on the genus. Zootaxa, 4500 (4), 574–580.
http://dx.doi.org/10.11646/zootaxa.4500.4.6
Cedrola, F., Senra, M.V.X., D’Agosto, M. & Dias, R.J.P. (2017) Phylogenetic analyses support validity of genus Eodinium (Ciliophora, Entodiniomorphida, Ophryoscolecidae). Journal of Eukaryotic Microbiology, 64, 242–247.
https://doi.org/10.1111/jeu.12355
Dehority, B.A. (1978) Specificity of rumen ciliate protozoa in cattle and sheep. Journal of Protozoology, 25, 509–513.
https://doi.org/10.1111/j.1550-7408.1978.tb04177.x|
Dehortiy, B.A. (1984) Evaluation of subsampling and fixation procedures used for counting rumen protozoa. Applied and Environmental Microbiology, 48 (1), 182–185.
Dehority, B.A. (1993) Laboratory Manual for Classification and Morphology of Rumen Ciliate Protozoa. CRC Press, Florida, 128 pp.
Dogiel, V.A. (1925) On the influence of the macronucleus on the formation of new morphological characters in infusoria. Quarterly Journal of Microscopical Science, 69, 611–617.
Dogiel, V.A. (1927) Monographie der familie Ophryoscolecidae. Archiv für Protistenkunde, 59, 1–288.
Eadie, J.M. (1962) Inter-relationships between certain rumen ciliate protozoa. Journal of General Microbiology, 29, 579–588.
https://doi.org/10.1099/00221287-29-4-579
Fernández-Galiano, T., Serrano, S. & Fernández-Galiano, D. (1985) General morphology and stomatogenesis of two species of the genus Entodinium (Ciliophora, Entodiniomorphida). Acta Protozoologica, 24, 181–186.
Franzolin, R. & Dehority, B.A. (1996) Effect of prolonged high-concentrate feeding on ruminal protozoa concentrations. Journal of Animal Science, 74, 2803–2809.
https://doi.org/10.2527/1996.74112803x
Göçmen, B., Dehority, B.A., Talu, G.H. & Rastgeldi, S. (2001) The rumen ciliate fauna of domestic sheep (Ovis ammon aries) from the Turkish Republic of Northern Cyprus. Journal of Eukaryotic Microbiology, 48 (4), 455–459.
https://doi.org/10.1111/j.1550-7408.2001.tb00179.x
Göçmen, B. & Gürelli, G. (2009a) Rumen entodiniid ciliated protozoan fauna (Entodiniomorphida: Entodiniidae) of domestic sheep (Ovis ammon aries L.) from Northern Cyprus, with a description of a new species, Entodinium cypriensis sp. nov. Turkish Journal of Zoology, 33, 169–180.
https://doi.org/10.3906/zoo-0807-5
Göçmen, B. & Gürelli, G. (2009b) The occurence of the rumen ciliate Entodinium constrictum Dehority, 1974 (Entodiniidae, Entodiniomorphida) from domestic sheep (Ovis ammon aries L.) in Northern Cyprus. North-Western Journal of Zoology, 5 (2), 301–306.
Göçmen, B., Torun, S. & Öktem, N. (1999) Türkiye evcil koyun (Ovis ammon aries)’larının işkembe siliyat (Protoza: Ciliophora) faunası hakkında bir ön çalışma: II- Familya Ophryoscolecidae (Entodiniomorphida). Doğa-Turkish Journal of Zoology, 23, 473–490.
Gruby, D. & Delafond, O. (1843) Recherches sur des animalcules su developpant en gland nombre dens I’estomac et les intestins, pendant la digestion des animaux herbivores et carnivores. Comptes Rendus Academy Science Paris, 17, 1304–1308.
Gürelli, G. (2014) Rumen ciliate fauna (Ciliophora, Protista) of Turkish domestic goats living in İzmir, Turkey. Turkish Journal of Zoology, 38, 136–143.
https://doi.org/10.3906/zoo-1303-21
Gürelli, G. (2016) Rumen ciliates of domestic cattle (Bos taurus taurus) in Kastamonu, Turkey with the description of a new species. European Journal of Protistology, 56, 51–59.
https://doi.org/10.1016/j.ejop.2016.07.002
Gürelli, G. (2017) Rumen ciliate fauna of domestic sheep (Ovis aries) in İzmir, Turkey and scanning electron microscopic observations. Zootaxa, 4286 (4), 545–554.
https://doi.org/10.11646/zootaxa.4286.4.6
Gürelli, G. (2018) Infraciliature of Eudiplodinium dilobum, E. rostratum, and E. maggii (Ciliophora, Entodiniomorphida). Commagene Journal of Biology, 2 (2), 16–18.
https://doi.org/10.31594/commagene.477309
Gürelli, G. & Akman, F.T.B. (2017) Rumen ciliate biota of domestic cattle (Bos taurus taurus) in İstanbul, Turkey and Infraciliature of Metadinium medium (Entodiniomorphida, Ophryoscolecidae). Acta Protozoologica, 56, 171–180.
https://doi.org/10.4467/16890027AP.17.015.7496
Gürelli, G., Canbulat, S., Aldayarov, N. & Dehority, B.A. (2016) Rumen ciliate protozoa of domestic sheep (Ovis aries) and goat (Capra aegagrus hircus) in Kyrgyzstan. FEMS Microbiology Letters, 363, fnw028.
https://doi.org/10.1093/femsle/fnw028
Hungate, R.E. (1996) The Rumen and Its Microbes. Academic Press, New York, 533 pp.
Imai, S., Han, S.S., Cheng, K.-J. & Kudo, H. (1989) Composition of the rumen ciliate population in experimental herds of cattle and sheep in Lethbridge, Alberta, Western Canada. Canadian Journal of Microbiology, 35 (7), 686–690.
https://doi.org/10.1139/m89-112
Imai, S., Tsutsumi, Y., Yumura, S. & Mulenga, A. (1992) Ciliate protozoa in the rumen of Kafue lechwe, Kobus leche kafuensis, in Zambia, with the description of four new species. Journal of Protozoology, 39 (5), 564–572.
https://doi.org/10.1111/j.1550-7408.1992.tb04852.x
Ito, A. & Imai, S. (1990) Ciliated protozoa in the rumen of holstein-friesian cattle (Bos taurus taurus) in Hokkaido, Japan, with the description of two new species. Zoological Science, 7, 449–458.
Ito, A. & Imai, S. (1998) Infraciliary bands in the rumen ophryoscolecid ciliate Ostracodinium gracile (Dogiel, 1925), observed by light microscopy. Journal of Eukaryotic Microbiology, 45, 628–636.
https://doi.org/10.1111/j.1550-7408.1998.tb04559.x|
Ito, A. & Imai, S. (2003) Light microscopical observation of infraciliary bands of Eodinium posterovesiculatum in comparison with Entodinium bursa and Diplodinium dentatum. Journal of Eukaryotic Microbiology, 50, 34–42.
https://doi.org/10.1111/j.1550-7408.2003.tb00103.x|
Ito, A. & Imai, S. (2006) Infraciliary band pattern of rumen ophryoscolecid ciliates. Endocytobiosis and Cell Research, 17, 103–110.
Ito, A., Imai, S. & Ogimoto, K. (1993) Rumen ciliates of ezo deer (Cervus nippon yesoensis) with the morphological comparison with those of cattle. Journal of Veterinary Medical Science, 55 (1), 93–98.
https://doi.org/10.1292/jvms.55.93
Kittelmann, S. & Janssen, P.H. (2011) Characterization of rumen ciliate community composition in domestic sheep, deer, and cattle, feding on varying diets, by means of PCR-DGGE and clone libraries. FEMS Microbiology Ecology, 75, 468–481.
https://doi.org/10.1111/j.1574-6941.2010.01022.x
Kittelmann, S., Seedorf, H., Walters, W.A., Clemente, J.C., Knight, R., Gordon, J.I. & Janssen, P.H. (2013) Simultaneous amplicon sequencing to explore co-occurrence patterns of bacterial, archaeal and eukaryotic microorganisms in rumen microbial communities. PLOS ONE, 8/2, e47879.
https://doi.org/10.1371/journal.pone.0047879
Kofoid, C.A. & MacLennan, R.F. (1932) Ciliates from Bos indicus. II. A revision of Diplodinium. University California Publications in Zoology, 37, 53–152.
Lynn, D.H. (2008) The Ciliated Protozoa, Characterization, Classification and Guide to the Literature. 3rd Edition. Springer, New York, 605 pp.
Moon-van der Staay, S.Y., van der Staay, G.W.M., Michalowski, T., Jauany J.-P., Pristas, P., Javorský, P., Kišidayová, S., Varadyova, Z., McEwan, N.R., Newbold, C.J., van Alen, T., de Graaf, R., Schmid, M., Huynen, M.A. & Hackstein, J.H.P. (2014) The symbiotic intestinal ciliates and the evolution of their hosts. European Journal of Protistology, 50, 166–173.
https://doi.org/10.1016/j.ejop.2014.01.004
Ogimoto, K. & Imai, S. (1981) Atlas of Rumen Microbiology. Japan Scientific Societies Press, Tokyo, 231 pp.
Öktem, N., Göçmen, B. & Torun, S. (1997) Türkiye evcil koyun (Ovis ammon aries)’larının işkembe siliyat (Protozoa: Ciliophora) faunası hakkında bir ön çalışma: I. Familya Isotrichidae (Trichostomatida) ve Entodiniidae (Entodiniomorphida). Turkish Journal of Zoology, 21, 475–502.
Selim, H.M., Imai, S., Yamato, O., El Kabbany, A., Kiroloss, F. & Maede, Y. (1996) Comparative study of rumen ciliates in buffalo, cattle and sheep in Egypt. Journal of Veterinary Medical Science, 58 (8), 799–801.
https://doi.org/10.1292/jvms.58.799
Selim, H.M., Imai, S., El Sheik, A.K., Attia, H., Okamoto, E., Miyagawa, E. & Maede, Y. (1999) Rumen ciliate protozoal fauna of native sheep, Friesian cattle and dromedary camel in Libya. Journal of Veterinary Medical Science, 61 (3), 303–305.
https://doi.org/10.1292/jvms.61.303
Vďačný, P. (2018) Evolutionary associations of endosymbiotic ciliates shed light on the timing of the marsupial-placental split. Molecular Biology and Evolution, 35 (7), 1757–1769.
https://doi.org/10.1093/molbev/msy071
Williams, A.G. & Coleman, G.S. (1992) The Rumen Protozoa. Springer, New York, 442 pp.
https://doi.org/10.1007/978-1-4612-2776-2