Abstract
A new species of the genus Pedesta Hemming, 1934, viz. P. wangi sp. nov., is described as new to science from northwestern Guangxi, southern China. Based upon 455 bp partial COI sequences, a neighbor-joining (NJ) tree including the new species and twelve congeneric species is reconstructed. It shows that P. wangi sp. nov. is a cryptic species of P. xiaoqingae (Huang & Zhan, 2004), and the mean Kimura-2-Parameter distance between them reaches 2.8%. Wing patterns and genitalic structures of the males of the two species are illustrated and compared. In addition, three female samples are associated with P. xiaoqingae male in the NJ tree, and its wing pattern and genitalia are illustrated and described herein for the first time.
References
Grishin, N.V., Janzen, D.H. & Hallwachs, W. (2014) A new species of Eracon (Hesperiidae: Pyrginae) substantiated by a number of traits, including female genitalia. Journal of the Lepidopterists’ Society, 68 (3), 149–161.
https://doi.org/10.18473/lepi.v68i3.a1
Hebert, P.D.N., Cywinska, A., Ball, S.L. & deWaard, J.R. (2003) Biological identifications through DNA barcodes. Proceedings of Royal Society, Biology Sciences, 270, 313–322.
https://doi.org/10.1098/rspb.2002.2218
Huang, H. & Zhan, C.H. (2004) Notes on the genera Thoressa and Pedesta, with description of a new species from south China. Neue Entomologische Nachrichten, 57, 179–186.
Huang, Z.F., Chiba, H., Jin, J., Kizhakke, A.G., Wang, M., Kunte, K. & Fan, X.L. (2019) A multilocus phylogenetic framework of the tribe Aeromachini (Lepidoptera: Hesperiidae: Hesperiinae), with implications for taxonomy and biogeography. Systematic Entomology, 44, 163–178.
https://doi.org/10.1111/syen.12322
Inayoshi, Y. (2019) A check list of butterflies in Indo-China, chiefly from Thailand, Laos & Vietnam. Available from: http://yutaka.it-n.jp/hes/91247001.html (accessed 2 February 2019)
Rand, D.B., Heath, A., Suderman, T. & Pierce, N.E. (2000) Phylogeny and life history evolution of the genus Chrysoritis within the Aphnaeini (Lepidoptera: Lycaenidae), inferred from mitochondrial cytochrome oxidase I sequences. Molecular Phylogenetic and Evolution, 17, 85–96.
https://doi.org/10.1006/mpev.2000.0820
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. (1997) The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25, 4876–4882.
https://doi.org/10.1093/nar/25.24.4876
Zhai, Q., Xue, G.X. & Li, M. (2017) DNA barcoding-based sexual association of Sovia lucasii and S. lii (Lepidoptera: Hesperiidae), with description of a new subspecies. PLoS ONE, 12 (8), e0183847.
https://doi.org/10.1371/journal.pone.0183847
Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33 (7), 1870–1874.
https://doi.org/10.1093/molbev/msw054