Abstract
A new species of the genus Cyrtodactylus from Vientiane Province, northern Laos is described based on morphological and molecular data. Cyrtodactylus muangfuangensis sp. nov. can be distinguished from remaining congeners by the following combination of characters: maximum SVL 83.9 mm; dorsal pattern consisting of dark nuchal loop, nape band and five dark transversal bands between limb insertions; intersupranasals two; dorsal tubercles present on occiput, body, hind limbs and tail base; 15 or 16 irregular dorsal tubercle rows at midbody; lateral folds clearly defined, without interspersed tubercles; 31–37 ventral scales between ventrolateral folds; six precloacal pores and 15 femoral pores in males, which are interrupted by six to eight poreless scales; six precloacal pitted scales plus in total 10–15 pitted femoral scales in females, which are separated by six to eight poreless scales; enlarged precloacal and femoral scales present; two or three postcloacal tubercles; median subcaudal scales transversely enlarged. In molecular analyses, the new species is strongly supported as a member of the Cyrtodactylus phongnhakebangensis species group, and weakly corroborated as a sister taxon to C. pageli. Pairwise genetic comparison shows that it is at least 18% divergent from other congeners in the species group based on a fragment of the mitochondrial cytochrome C oxidase subunit I gene.
References
Bauer, A.M. & Henle, K. (1994) Familia Gekkonidae (Reptilia, Sauria). Part 1 Australia and Oceania. Das Tierreich 109 (part). Walter de Gruyter, Berlin, xiii + 306 pp.
https://doi.org/10.1515/9783110885958
Bauer, A., Kunya, K., Sumontha, M., Niyomwan, P., Pauwels, O.S.G., Chanhome, L. & Kunya, T. (2010) Cyrtodactylus dumnuii (Squamata: Gekkonidae), a new cave-dwelling gecko from Chiang Mai Province, Thailand. Zootaxa, 2570 (1), 41–50.
https://doi.org/10.11646/zootaxa.2570.1.2
Bauer, A.M., Sumontha, M. & Pauwels, O.S.G. (2003) Two new species of Cyrtodactylus (Reptilia: Squamata: Gekkonidae) from Thailand. Zootaxa, 376 (1), 1–18.
https://doi.org/10.11646/zootaxa.376.1.1
Ivanova, N.V., de Waard, J. & Hebert, P.D.N. (2006) An inexpensive, automation-friendly protocol for recovering high-quality DNA. Molecular Ecology Notes, 6, 998–1002.
https://doi.org/10.1111/j.1471-8286.2006.01428.x
Le, D.T., Nguyen, Q.T., Le, M.D. & Ziegler, T. (2016) A new species of Cyrtodactylus (Squamata: Gekkonidae) from Ninh Binh Province, Vietnam. Zootaxa, 4162 (2), 268–282.
https://doi.org/10.11646/zootaxa.4162.2.4
Le, M., Raxworthy, C.J., McCord, W.P. & Mertz, L. (2006) A molecular phylogeny of tortoises (Testudines: Testudinidae) based on mitochondrial and nuclear genes. Molecular Phylogenetics and Evolution, 40, 517−531.
https://doi.org/10.1016/j.ympev.2006.03.003
Luu, V.Q., Bonkowski, M., Nguyen, T.Q., Le, M.D., Schneider, N., Ngo, H.T. & Ziegler, T. (2016a) Evolution in karst massifs: Cryptic diversity among bent-toed geckos along the Truong Son Range with descriptions of three new species and one new country record from Laos. Zootaxa, 4107 (2), 101–140.
https://doi.org/10.11646/zootaxa.4107.2.1
Luu, V.Q., Calame, T., Bonkowski, M., Nguyen, Q. T. & Ziegler, T. (2014) A new species of Cyrtodactylus (Squamata: Gekkonidae) from Khammouane Province, Laos. Zootaxa, 3760 (1), 54−66.
https://doi.org/10.11646/zootaxa.3760.1.3
Luu, V.Q., Calame, T., Nguyen, T.Q., Bonkowski, M. & Ziegler, T. (2015) A new species of Cyrtodactylus (Squamata: Gekkonidae) from limestone forest, Khammouane Province, central Laos. Zootaxa, 4058 (3), 388–402.
https://doi.org/10.11646/zootaxa.4058.3.6
Luu, V.Q., Calame, T., Nguyen, T.Q., Le, M.D., Bonkowski, M. & Ziegler, T. (2016b) Cyrtodactylus rufford, a new cave-dwelling bent-toed gecko (Squamata: Gekkonidae) from Khammouane Province, central Laos. Zootaxa, 4067 (2), 185–199.
https://doi.org/10.11646/zootaxa.4067.2.4
Luu, V.Q., Dung, T.V., Nguyen, T.Q., Le, M.D. & Ziegler, T. (2017) A new species of the Cyrtodactylus irregularis complex (Squamata: Gekkonidae) from Gia Lai Province, Central Highlands. Zootaxa, 4362 (3), 385–404.
https://doi.org/10.11646/zootaxa.4362.3.4
Luu, V.Q., Nguyen, T.Q., Do, H.Q. & Ziegler, T. (2011) A new Cyrtodactylus (Squamata: Gekkonidae) from Huong Son limestone forest, Hanoi, northern Vietnam. Zootaxa, 3129 (1), 39–50.
https://doi.org/10.11646/zootaxa.3129.1.3
Luu, V.Q., Nguyen, T.Q., Le, M.D., Bonkowski, M. & Ziegler, T. (2016c) A new species of karst-dwelling bent-toed gecko (Squamata: Gekkonidae) from Khammouane Province, central Laos. Zootaxa, 4079 (1), 87–102.
https://doi.org/10.11646/zootaxa.4079.1.6
Murdoch, M.L., Grismer, L.L., Wood JR, P.L., Neang, T., Poyarkov, N.A., Ngo, T.V., Nazarov, R.A., Aowphol, A., Pauwels, O.S.G., Nguyen, H.N. & Grismer, J.L. (2019) Six new species of the Cyrtodactylus intermedius complex (Squamata: Gekkonidae) from the Cardamom Mountains and associated highlands of Southeast Asia. Zootaxa, 4554 (1), 1–62.
https://doi.org/10.11646/zootaxa.4554.1.1
Nazarov, R.A., Pauwels, O.S.G., Konstantinov, E.L., Chulisov, A.S., Orlov, N.L. & Poyarkov, N.A. (2018) A new Karst-dwelling bent-toed gecko (Squamata: Gekkonidae: Cyrtodactylus) from Xiangkhoang Province, northeastern Laos. Zoological Research, 39 (3), 197–213.
Nazarov, R.A., Poyarkov, N.A., Orlov, N.L., Nguyen, S.N., Milto, K.D., Martynov, A.A., Konstantinov, E.L. & Chulisov, A.S. (2014) A review of genus Cyrtodactylus (Reptilia: Sauria: Gekkonidae) in fauna of Laos with description of four new species. Proceedings of the Zoological Institute RAS, 318, 391–423.
Nguyen, L.-T., Schmidt, H.A., von Haeseler, A. & Bui, M.Q. (2015a) IQ-TREE: A fast and effective stochastic algorithm for estimating maximum likelihood phylogenies. Molecular Biology and Evolution, 32, 268−274.
https://doi.org/10.1093/molbev/msu300
Nguyen, S.N., Yang, J.-X., Le, T.-N.T., Nguyen, L.T., Orlov, N.L., Hoang, C.V., Nguyen, T.Q., Jin, J.-Q., Rao, D.-Q., Hoang, T.N., Che, J., Murphy, R.W. & Zhang, Y.-P. (2014) DNA barcoding of Vietnamese bent-toed geckos (Squamata: Gekkonidae: Cyrtodactylus) and the description of a new species. Zootaxa, 3784 (1), 48–66.
https://doi.org/10.11646/zootaxa.3784.1.2
Nguyen, T.Q., Le, M.D., Pham, A.V., Ngo, H.N., Hoang, C.V., Pham, C.T. & Ziegler, T. (2015b) Two new species of Cyrtodactylus (Squamata: Gekkonidae) from the karst forest of Hoa Binh Province, Vietnam. Zootaxa, 3985 (3), 375–390.
https://doi.org/10.11646/zootaxa.3985.3.3
Nguyen, T.Q., Pham, A.V., Ziegler, T., Ngo, H.T. & Le, M.D. (2017) A new species of Cyrtodactylus (Squamata: Gekkonidae) and the first record of C. otai from Son La Province, Vietnam. Zootaxa, 4341 (1), 25–40.
https://doi.org/10.11646/zootaxa.4341.1.2
Panitvong, N., Sumontha, M., Tunprasert, J. & Pauwels, O.S.G. (2014) Cyrtodactylus saiyok sp. nov., a new dry evergreen forest-dwelling Bent-toed Gecko (Squamata: Gekkonidae) from Kanchanaburi Province, western Thailand. Zootaxa, 3869 (1), 064–074. https://doi.org/10.11646/zootaxa.3869.1.6
Pauwels, O.S.G., Nazarov, R.A., Bobrov, V.V. & Poyarkov, N.A. (2018) Taxonomic Status of two populations of Bent-toed Geckos of the Cyrtodactylus irregularis complex (Squamata: Gekkonidae) with description of a new species from Nui Chua National Park, southern Vietnam. Zootaxa, 4403 (2), 307–335.
https://doi.org/10.11646/zootaxa.4403.2.5
Pauwels, O.S.G. & Sumontha, M. (2014) Cyrtodactylus samroiyot, a new limestone-dwelling Bent-toed Gecko (Squamata: Gekkonidae) from Prachuap Khiri Khan Province, peninsular Thailand. Zootaxa, 3755 (6), 573–583.
https://doi.org/10.11646/zootaxa.3755.6.4
Pauwels, O.S.G., Sumontha, M., Panitvong,N. & Varaguttanonda, V. (2014) Cyrtodactylus khelangensis, a new cave-dwelling Bent-toed Gecko (Squamata: Gekkonidae) from Lampang Province, northern Thailand. Zootaxa, 3755 (6), 584–594.
https://doi.org/10.11646/zootaxa.3755.6.5
Pauwels, O.S.G., Sumontha, M. & Bauer, A.M. (2016) A new Bent-toed Gecko (Squamata: Gekkonidae: Cyrtodactylus) from Phetchaburi Province, Thailand. Zootaxa, 4088 (3), 409–419.
https://doi.org/10.11646/zootaxa.4088.3.6
Pham, A.V., Le, M.D., Ngo, H.T., Ziegler, T. & Nguyen, T.Q. (2019) A new species of Cyrtodactylus (Squamata: Gekkonidae) from northwestern Vietnam. Zootaxa, 4544 (1), 360–380.
https://doi.org/10.11646/zootaxa.4544.3.3
Posada, D. & Crandall, K.A. (1998) MODELTEST: testing the model of DNA substitution. Bioinformatics, 14, 817−818.
https://doi.org/10.1093/bioinformatics/14.9.817
Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61, 539−542.
https://doi.org/10.1093/sysbio/sys029
Schneider, N., Nguyen, T.Q., Le, M.D, Nophaseud, L., Bonkowski, M. & Ziegler, T. (2014a) A new species of Cyrtodactylus (Squamata: Gekkonidae) from the karst forest of northern Laos. Zootaxa, 3835 (1), 80–96.
https://doi.org/10.11646/zootaxa.3835.1.4
Schneider, N., Phung, T.M., Le, M.D., Nguyen, T.Q. & Ziegler, T. (2014b) A new Cyrtodactylus (Squamata: Gekkonidae) from Khanh Hoa Province, southern Vietnam. Zootaxa, 3785 (4), 518–532.
https://doi.org/10.11646/zootaxa.3785.4.2
Simmons, J.E. (2002) Herpetological collecting and collections management. Revised edition. Society for the Study of Amphibians and Reptiles, Herpetological Circular, 31, 1–153.
Smith, M.A. (1923) Notes on reptiles and batrachians from Siam and Indo–China (No. 2). Journal of Natural History Society of Siam, 6, 47–53.
Stuart, B.L. (1999) Amphibians and reptiles. In: Duckworth, J.W., Salter, R.E. & Khounboline, K. (Eds.), Wildlife in Lao PDR: 1999 status report. IUCN, WCS & CPAWM, Vientiane, pp. 43–67.
Sumontha, M., Pauwels, O.S.G., Panitvong, N., Kunya, K. & Grismer, J.L. (2015) A new lowland forest Bent-toed Gecko (Squamata: Gekkonidae: Cyrtodactylus) from Ranong Province, peninsular Thailand. Zootaxa, 3911 (1), 106–118.
https://doi.org/10.11646/zootaxa.3911.1.6
Sumontha, M., Pauwels, O.S.G., Suwannakarn, N., Nutatheera, T. & Sodob, W. (2014) Cyrtodactylus wangkulangkulae (Squamata: Gekkonidae) a new Bent-toed Gecko from Satun Province, Southern Thailand. Zootaxa, 3821 (1), 116–124.
https://doi.org/10.11646/zootaxa.3821.1.8
Swofford, D.L. (2001) PAUP*. Phylogenetic Analysis Using Parsimony (* and Other Methods). Version 4. Sinauer Associates, Sunderland, Massachusetts. [program]
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. (1997) The ClustalX windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25, 4876−4882.
https://doi.org/10.1093/nar/25.24.4876
Uetz, P., Freed, P. & Hošek, J. (Eds.) (2019) The Reptile Database. Availabe from: http://www.reptile-database.org (accessed 24 April 2019)