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Type: Article
Published: 2020-01-13
Page range: 185–194
Abstract views: 141
PDF downloaded: 6

A new species of Diphascon (Tardigrada: Hypsibiidae) from Northern China supported by integrated taxonomy

College of Life Sciences, Northeast Forestry University, Harbin, 150001, Heilongjiang, China
College of Wildlife Resource, Northeast Forestry University, Harbin, 150001, Heilongjiang, China
College of Life Sciences, Northeast Forestry University, Harbin, 150001, Heilongjiang, China
College of Life Sciences, Northeast Forestry University, Harbin, 150001, Heilongjiang, China
School of Material Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010, Sichuan, China
Tardigrada 18S rRNA China COI Diphascon wuyingensis sp. nov.

Abstract

Morphological and molecular analyses have determined that there is a new species of Tardigrada found in China. Diphascon wuyingensis sp. nov., has smooth cuticle, pharyngeal apophyses, three rod-shaped macroplacoids (increasing in length from first to third, with the second macroplacoid clearly longer than the first) and lacks microplacoids and septulum. The new species has a very small drop-shaped formation and small claws of the Hypsibius type, but no pseudolunules or other cuticular thickenings. Three individual specimens and a group of four specimens were used for DNA isolation and 18S rRNA and COI sequencing; the p-distances to another three Diphascon species used for comparison varied in ranges of 8.8–10.2% (18S rRNA) and 24.2–26.7% (COI).

 

References

  1. Bartoš, E. (1935) Vier neue Hypsibiusarten aus der Tschechoslowakie. Zoologischer Anzeiger, 110, 257–260.

    Beasley, C.W., Kaczmarek Ł. & Michalczyk, Ł. (2008) Doryphoribius mexicanus, a new species of Tardigrada (Eutardigrada: Hypsibiidae) from Mexico (North America). Proceedings of the Biological Society of Washington, 121 (1), 34–40.

    https://doi.org/10.2988/07-30.1

    Beasley, C.W. & Miller, W.R. (2012) Additional Tardigrada from Hubei Province, China, with the description of Doryphoribius barbarae sp. nov. (Eutardigrada: Parachela: Hypsibiidae). Zootaxa, 3170 (1), 55–63.

    https://doi.org/10.11646/zootaxa.3170.1.5

    Bertolani, R., Guidetti, R., Marchioro, T., Altiero, T., Rebecchi, L. & Cesari, M. (2014) Phylogeny of Eutardigrada: new molecular data and their morphological support lead to the identification of new evolutionary lineages. Molecular Phylogenetics and Evolution, 76, 110–126.

    https://doi.org/10.1016/j.ympev.2014.03.006

    Binda, M.G. (1971) Su alcuni tardigradi muscicoli del Nord-Africa. Bollettino delle sedute della Accademia Gioenia di scienze naturali in Catania, 10, 759–765.

    Binda, M.G., Pilato, G. & Dastych, H. (1984) Diphascon modestum, nuova specie di eutardigrado dei monti tatra. Animalia, 11, 45–48.

    Binda, M.G. & Pilato, G. (1969) Ulteriore contributo alla conoscenza dei Tardigradi di Sicilia con descrizione di due nuove specie. Bollettino delle sedute dell’Accademia Gioenia di Scienze Naturali, Catania, IV (X), 205–214.

    Chen, C.X., Li, G.P. & Sun, P.F. (2013)The analysis to the temperature changing trend and asymmetry of Wuying region. Heilognjiang Meteorology, 30 (3), 15–17.

    Cesari, M., Bertolani, R., Rebecchi, L. & Guidetti, R. (2009) DNA barcoding in Tardigrada: the first case study on Macrobiotus macrocalix Bertolani & Rebecchi 1993 (Eutardigrada, Macrobiotidae). Molecular Ecology Resources, 9 (3), 699–706.

    https://doi.org/10.1111/j.1755-0998.2009.02538.x

    Dastych (1992) Paradiphascon manningi gen. n. sp. n., a New Water-Bear from South Africa, with the erecting of a new subfamily Diphasconinae (Tardigrada). Mitteilungen aus dem Hamburgischen Zoologischen Museum und Institut, 89, 125–139.

    Degma, P. & Guidetti, R. (2007) Notes to the current checklist of Tardigrada. Zootaxa, 1579 (1), 41–53.

    https://doi.org/10.11646/zootaxa.1579.1.2

    Degma, P., Bertolani, R. & Guidetti, R. (2009–2018) Actual checklist of Tardigrada species. 48 pp. Available from: http://www.tardigrada.modena.unimo.it/miscellanea/Actual%20checklist%20of%20Tardigrada.pdf (accessed 11 September 2018)

    Doyère, M. (1840) Mémoire sur les Tardigrades. Annales des sciences naturelles, Paris, Supplement 2 (14), 269–361.

    Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3 (5), 294–299.

    Gao, X.Y., Li, X.C. &Wang, L.Z. (2012) Taxonomic composition of the Chinese terrestrial-freshwater tardigrades. Journal of Anhui Agriculture Sciences, 40 (18), 9721–9725.

    Gąsiorek, P., Zawierucha, K., Stec, D. & Michalczyk, Ł. (2017) Integrative redescription of a common Arctic water bear Pilatobius recamieri (Richters, 1911). Polar Biology, 40 (11), 2239–2252.

    https://doi.org/10.1007/s00300-017-2137-9

    Guidetti, R. & Bertolani, R. (2005) Tardigrade taxonomy: an updated check list of the taxa and a list of characters used for their identification. Zootaxa, 845 (1), 1–46.

    https://doi.org/10.11646/zootaxa.845.1.1

    Guidetti, R. & Bertolani, R. (2011) Phylum Tardigrada Doyère, 1840. In: Zhang, Z.-Q. (Ed.), Animal biodiversity: An outline of higher-level classification and survey of taxonomic richness. Zootaxa, 3148 (1), pp. 96–97.

    [https://www.zin.ru/animalia/coleoptera/pdf/zt03148pp1-208.pdf]

    https://doi.org/10.11646/zootaxa.3148.1.12

    Guidetti, R., Schill, R.O., Bertolani, R., Dandekar, T. & Wolf, M. (2009) New molecular data for tardigrade phylogeny, with the erection of Paramacrobiotus gen. nov. Journal of Zoological Systematics and Evolutionary Research, 47 (4), 315–321.

    https://doi.org/10.1111/j.1439-0469.2009.00526.x

    Guidetti, R., Peluffo, J.R., Rocha, A.M., Cesari, M. & de Peluffo, M.C. (2013) The morphological and molecular analyses of a new South American urban tardigrade offer new insights on the biological meaning of the Macrobiotus hufelandi group of species (Tardigrada: Macrobiotidae). Journal of Natural History, 47 (37–38), 2409–2426.

    https://doi.org/10.1080/00222933.2013.800610

    Guil, N. & Giribet, G. (2011) A comprehensive molecular phylogeny of tardigrades-adding genes and taxa to a poorly resolved phylum-level phylogeny. Cladistics, 28 (1), 21–49.

    https://doi.org/10.1111/j.1096-0031.2011.00364.x

    Guil, N., Jørgensen, A. & Kristensen, R. (2019) An upgraded comprehensive multilocus phylogeny of the Tardigrada tree of life. Zoologica Scripta, 48 (1),120–137.

    https://doi.org/10.1111/zsc.12321

    Jennings, P.G. (1976)Tardigrada of Signy Island, South Orkney Islands, with a note on the Rotifera. British Antarctic Survey Bulletin, 44, 1–25.

    Jin, S.B., Wang, C., Cheng, C.X. & Sun, P.F. (2017) Factual analysis of climate change in the past 58 years in the Wuying Forest Region. Heilongjiang Meteorology, 34 (2), 7–10.

    Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33 (7), 1870–1874.

    https://doi.org/10.1093/molbev/msw054

    Kaczmarek, Ł., Cytan, J., Zawierucha, K., Diduszko, D. & Michalczyk, Ł. (2014) Tardigrades from Peru (South America), with descriptions of three new species of Parachela. Zootaxa, 3790 (2), 357–379.

    https://doi.org/10.11646/zootaxa.3790.2.5

    Kosztyła, P., Stec, D., Morek, W., Gąsiorek, P., Zawierucha, K., Michno, K., Ufir, K., Małek, D., Hlebowicz, K., Laska, A., Dudziak, M., Frohme, M., Prokop, Z.M., Kaczmarek, Ł. & Michalczyk, Ł. (2016) Experimental taxonomy confirms the environmental stability of morphometric traits in a taxonomically challenging group of microinvertebrates. Zoological Journal of the Linnean Society, 178 (4), 765–775.

    https://doi.org/10.1111/zoj.12409

    Marcus, E. (1936) Tardigrada. In: De Gruyter, W. (Ed.), Das Tierreichs, 66, 1–340.

    Medlin, L., Elwood, H.J., Stickel, S. & Sogin, M.L. (1988) The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Gene, 71 (2), 491–499.

    https://doi.org/10.1016/0378-1119(88)90066-2

    Meng, H.Z., Sun, X.Z. & Li, X.C. (2014) A new species of the genus Doryphoribius (Eutardigrada: Isohypsibiidae) from China. Proceedings of the Biological Society of Washington, 127 (3), 510–517.

    https://doi.org/10.2988/0006-324X-127.3.510

    Michalczyk, Ł. & Kaczmarek, Ł. (2013) The Tardigrada Register: a comprehensive online data repository for tardigrade taxonomy. Journal of Limnology, 72 (S1), 175–181.

    https://doi.org/10.4081/jlimnol.2013.s1.e22

    Mihelčič, F. (1971) Beobachtungen an Tardigraden Osttirols (I). Veroffentlichungen des Museum Ferdinandeum, 51, 119–140.

    Moly de Peluffo, M.C., Peluffo, J.R., Rocha, A.M. & Doma, I.L. (2006) Tardigrade distribution in a medium-sized city of Central Argentina. Hydrobiologia, 558 (1), 141–150.

    https://doi.org/10.1007/s10750-005-1413-9

    Pilato, G. (1969) Evoluzione e nuova sistemazione degli Eutardigrada. Bollettino di Zoologia, 36 (3), 327–345.

    https://doi.org/10.1080/11250006909436925

    Pilato, G. (1981) Analisi di nuovi caratteri nello studio degli Eutardigradi. Animalia, 8, 51–57.

    Pilato, G. (1987) Revision of the genus Diphascon Plate, 1889, with remarks on the subfamily Itaquasconinae (Eutardigrada, Hypsibiidae). In: Bertolani, R. (Ed.), Biology of Tardigrades. Selected Symposia and Monographs U.Z.I., 1, Mucchi, Modena, pp. 337–357.

    Pilato, G. (2009) Bindius triquetrus gen. n. sp. n. (Eutardigrada, Hypsibiidae) from Sicily (Italy). Zootaxa, 2058 (1), 62–68.