Abstract
A new species of the genus Limnophyes Eaton, 1875 from Xizang, China is described and figured based on adult males. Limnophyes nudus sp. nov. belongs to the minimus group and can be easily separated from other species by antenna with 13 flagellomeres, higher AR of 0.85–1.00, bluntly triangular anal point, phallapodeme with ring-like appendix, inferior volsella bare and gonostylus with bubble-like inner margin.
References
- Ashe, P. & O'Connor, J.P. (2012) A world catalogue of Chironomidae (Diptera). Part 2. Orthocladiinae. Irish Biogeographical Society & National Museum of Ireland, Dublin, 968 pp. - Cranston, P.S., Hardy, N.B. & Morse, G.E. (2012) A dated molecular phylogeny for the Chironomidae (Diptera). Systematic Entomology, 37, 172–188. - https://doi.org/10.1111/j.1365-3113.2011.00603.x - Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294–299. - Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41, 95–98. - Hebert, P.D.N., Ratnasingham, S. & de Waard, J.R. (2003) Barcoding animal life: cytochrome c oxidase subunit I divergences among closely related species. Proceedings of the Royal Society B-Biological Sciences, 270, S96–99. - https://doi.org/10.1098/rsbl.2003.0025 - Lin, X.L., Stur, E. & Ekrem, T. (2015) Exploring genetic divergence in a species-rich insect genus using 2790 DNA barcodes. PLoS One, 10, e0138993. - https://doi.org/10.1371/journal.pone.0138993 - Lin, X.L., Stur, E. & Ekrem, T. (2018) DNA barcodes and morphology reveal unrecognized species in Chironomidae (Diptera). Insect Systematics & Evolution, 49, 329–398. - https://doi.org/10.1163/1876312X-00002172 - Mendes, H.F., Andersen, T. & Pinho, L.C. (2007) Corytibacladius Oliveira, Messias & Santos, 1995, a junior synonym of Limnophyes Eaton, 1875 (Diptera: Chironomidae: Orthocladiinae). Aquatic Insects, 29, 255–261. - https://doi.org/10.1080/01650420701411481 - Moubayed, B.J. (2011) Description of Limnophyes tyrrheneus sp. n. from pozzines of the mountain lake Ninu in Corsica [Diptera, Chironomidae, Orthocladiinae]. Ephemera, 13 (2), 71–78. - Murray D.A. (2007) Limnophyes platystylus new species (Diptera: Chironomidae, Orthocladiinae) from Ireland. In: Andersen, T. (Ed.), Contributions to the Systematics and Ecology of Aquatic Diptera. A Tribute to Ole A. Sæther. The Caddis Press, Columbus, Ohio, pp. 215–218. - Pinho, L.C. & Andersen, T. (2015) Limnophyes guarani sp. n. a new hygropetric Orthocladiinae from southern Brazil (Diptera: Chironomidae). Zootaxa, 3948 (1), 137–144. - https://doi.org/10.11646/zootaxa.3948.1.9 - Przhiboro, A. & Sæther, O.A. (2007) Limnophyes (Diptera : Chironomidae) from northwestern Russia. Aquatic Insects, 29, 49–58. - https://doi.org/10.1080/01650420701225691 - Ree, H.I. (2013) Six new and two newly recorded species of Chironomidae (Insecta: Diptera) in Korea. Entomological Research, 43 (6), 322–329. - https://doi.org/10.1111/1748-5967.12037 - Sæther, O.A. 1969. Some Nearctic Podonominae, Diamesinae, and Orthocladiinae (Diptera: Chironomidae). Bulletin of the Fisheries Research Board of Canada, 170, 1–154. - Sæther, O.A. (1975) Twelve new species of Limnophyes Eaton, with keys to Nearctic males of the genus (Diptera: Chironomidae). The Canadian Entomologist, 107, 1029–1056. - https://doi.org/10.4039/Ent1071029-10 - Sæther, O.A. (1977) Female genitalia in Chironomidae and other Nematocera: morphology, phylogenies, keys. Bulletin of the Fisheries Research Board of Canada, 197, 1–209. - Sæther, O.A. (1980) Glossary of chironomid morphology terminology (Diptera: Chironomidae). Entomologica scandinavica, 14 (Supplement), 1–51. - Sæther, O.A. (1985) Heleniella parva sp. n. (Diptera: Chironomidae) from South Carolina and Tennessee, U.S.A. Entomologica Scandinavica, 15, 532–535. - https://doi.org/10.1163/187631284X00370 - Sæther, O.A. (1990a) A review of the genus Limnophyes Eaton from the Holarctic and Afrotropical regions. Entomologica scandinavica, 21, 305–319. - https://doi.org/10.1163/187631290X00229 - Sæther, O.A. (1990b) A revision of the Neotropical types described as Spaniotoma (Limnophyes) by Edwards 1931, with the description of Edwardsidia gen. n. (Diptera: Chironomidae). Entomologica scandinavica, 21 (3), 1–35. - https://doi.org/10.1163/187631290X00229 - Song, C., Lin, X.L., Wang, Q. & Wang, X.H. (2018) DNA barcodes successfully delimit morphospecies in a superdiverse insect genus. Zoologica Scripta, 47, 311–324. - https://doi.org/10.1111/zsc.12284 - Song, C., Wang, Q., Zhang, R.L., Sun, B.J. & Wang, X.H. (2016) Exploring the utility of DNA barcoding in species delimitation of Polypedilum (Tripodura) non-biting midges (Diptera: Chironomidae). Zootaxa, 4079 (5), 534–550. - https://doi.org/10.11646/zootaxa.4079.5.2 - Sublette, J.E. & Sasa, M. (1994) Chironomidae collected in Onchocerciasis endemic areas in Guatemala (Insecta, Diptera). Spixiana, 20 (Supplement), 1–60. - Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology Evolution, 30, 2725–2729. - https://doi.org/10.1093/molbev/mst197 - Wang, X. (1997) A new species of Limnophyes Eaton from China (Diptera: Chironomidae). Acta Scientiarum Naturalium University Nankaiensis, 30 (4), 5–7. - Wang, X. & Sæther, O.A. (1993) Limnophyes Eaton from China, with description of five new species (Diptera: Chironomidae). Entomologica scandinavica, 24, 215–226. - https://doi.org/10.1163/187631293X00316 
 
		