Abstract
Mosquitoes (Diptera: Culicidae) are insects capable of transmitting various disease agents. They have a wide geographical range, with a higher diversity and distribution in the tropical and subtropical regions. In Romania, data on mosquito distribution are available mostly from the older literature and published in local journals; hence, it is inaccessible to the international scientific community. The aim of this review was to gather all the published data on mosquitoes in Romania by performing a comprehensive literature search, to create a publicly available georeferenced database for the distribution of mosquitoes and generate distribution maps to identify gaps in the knowledge of mosquito diversity and distribution in the country. The resultant database includes 1,509 unique records for 51 mosquito species representing seven genera: Aedes, Anopheles, Culex, Culiseta, Coquillettidia, Orthopodomyia and Uranotaenia. Knowledge of mosquito distributions is important for understanding the risks of mosquito-borne pathogens present in Romania, a country with high endemicity for various diseases, such as West Nile fever and dirofilariasis. The database also revealed important knowledge gaps, including the lack of data for the Carpathian Arch and the central part of the historical regions of Moldova and Oltenia. The gaps become more evident, as the presence and diversity of the vectors have not been studied in many areas where diseases caused by mosquito-borne pathogens are present. In conclusion, despite the numerous georeferenced records, large areas of Romania remain unexplored for the mosquito species composition, abundance and phenology. This review is a “call for action” for implementing active surveillance in the country.
References
Aranda, C., Martínez, M. J., Montalvo, T., Eritja, R., Navero-Castillejos, J., Herreros, E., Marqués, E., Escosa, R., Corbella, I., Bigas, E., Picart, L., Jané, M., Barrabeig, I., Torner, N., Talavera, S., Vázquez, A., Sánchez-Seco, M.P. & Busquets, N. (2018) Arbovirus surveillance: first dengue virus detection in local Aedes albopictus mosquitoes in Europe, Catalonia, Spain, 2015. Eurosurveillance, 23 (47), pii=1700837.
https://doi.org/10.2807/1560-7917.ES.2018.23.47.1700837
Arbune, M. & Dobre, M. (2015) Dirofilariasis – an emergent human parasitosis in Romania. Acta Parasitologica, 60, 485–487.
https://doi.org/10.1515/ap-2015-0068
Becker, N., Petrić, D., Boase, C., Lane, J., Zgomba, M., Dahl, C. & Kaiser, A. (2010) Mosquitoes and their control. 2nd Edition. Springer-Verlag, Berlin and Heidelberg, xxx + 577 pp.
Beugnet, F. & Marié, J.L. (2009) Emerging arthropod-borne diseases of companion animals in Europe. Veterinary Parasitology, 163, 298–305.
https://doi.org/10.1016/j.vetpar.2009.03.028
Bîlbîe, I. & Nicolescu, G. (1986) Insecte vectoare și generatoare de discomfort. Editura Medicală, București, 344 pp.
Bîlbîe, I., Cristescu, A., Enescu, A., Tacu, V., Giurcă, I. & Cristodorescu-Nicolescu, G. (1978) Up-to-date entomological aspects in the previously endemic areas of malaria in the Danube plain and Dobrudja. Archives Roumaines de Pathologie experimentales et de Microbiologie, 37, 389–397.
Bîlbîe, I., Enescu, A., Giurcă, I., Nicolescu, G. & Velehorschi, N. (1983) Laboratory and field investigations of insecticide susceptibility of the anophelines in the previously endemic areas of the malaria in the Danube plain and Dobrudja. Archives Roumaines de Pathologie experimentales et de Microbiologie, 42, 343–351.
Braks, M., van der Giessen, J., Kretzschmar, M., van Pelt, W., Scholte, E.J., Reusken, C., Zeller, H., van Bortel, W. & Sprong, H. (2011) Towards an integrated approach in surveillance of vector-borne diseases in Europe. Parasites & Vectors, 4, 192.
https://doi.org/10.1186/1756-3305-4-192
Campbell, G.L., Ceianu, C.S. & Savagea, H.M. (2001) Epidemic West Nile encephalitis in Romania waiting for history to repeat itself. Annals of the New York Academy of Sciences, 951, 94–101.
https://doi.org/10.1111/j.1749-6632.2001.tb02688.x
Cancrini, G., Magi, M., Gabrielli, S., Arispici, M., Tolari, F., Dell’omodarme, M. & Prati, M.C. (2006) Natural vectors of dirofilariasis in rural and urban areas of the Tuscan Region, Central Italy. Journal of Medical Entomology, 43, 574–579.
https://doi.org/10.1093/jmedent/43.3.574
Centers for Disease Control and Prevention (2018) Lymphatic filariasis: Epidemiology & risk factors. Available from: https://www.cdc.gov/parasites/lymphaticfilariasis/epi.html (accessed 25 October 2018)
Ceianu, C., Ciolpan, O. & Nicolescu, G. (1995) Orthopodomyia pulcripalpis (Rondani, 1872) and Aedes geminus Peus, 1970 (Diptera: Culicidae)—two new species for the Romanian mosquito fauna. The 6th National Conference of General and Applied Entomology, Iași, Romania, 27–29 October, Oral presentation and submission unpublished and cited by Nicolescu (1995). Romanian Archives of Microbiology and Immunology, 54, 75–109.
Ciolpan, O., Nicolescu, G. & Pop, G. (1998) The mosquitoes (Diptera: Culicidae) in the area of the middle course of the river Someșul Mare (Romania): faunistical and ecological data. Roumanian Archives of Microbiology and Immunology, 57, 77–91.
Ciucă, M., Lupaşco, G., Duport, M., Săndulesco, M., Cristesco, A., Combinesco, I. & Săndulesco, I. (1964) Sensibilite et resistance des Anopheles [sic] vecteurs aux insecticides de contact apres 16 ans d’emloi du DDT et HCH en Roumanie. Archives Roumaines de Pathologie experimentales et de Microbiologie, 2, 211–218.
Cotar, A.I., Fălcuță, E., Dinu, S., Necula, A., Bîrluțiu, V., Ceianu, C.S. & Prioteasa, F.L. (2018) West Nile virus lineage 2 in Romania, 2015–2016: co-circulation and strain replacement. Parasites & Vectors, 11, 562.
https://doi.org/10.1186/s13071-018-3145-5
Cristesco, A. (1966) Contributions a l’etude de la composition par ages des populations du complexe Anopheles maculipennis par rapport a l’application des insecticides remanents en Roumanie. Archives Roumaines de Pathologie experimentales et de Microbiologie, 25, 491–502.
Cristescu, A., Duport, M., Tacu, V., Durbaca, S. & Iancu, L. (1975) Contribution to the study on the biology of Anopheles hyrcanus species from the Danube Delta. Archives Roumaines de Pathologie experimentales et de Microbiologie, 34, 277–284.
Danabalan, R., Monaghan, M.T., Ponsonby, D.J. & Linton, Y.-M. (2013) Occurrence and host preferences of Anopheles maculipennis group mosquitoes in England and Wales. Medical and Veterinary Entomology, 28, 169–178.
https://doi.org/10.1111/mve.12023
Dancescu, P. (1980) Aedines (Diptera: Culicidae) dans les enclaves boises de l’ambiance urbaine. Travaux du Musee d’Histoire Naturelle “Grigore Antipa”, Bucuresti, 22, 459–464.
Dinu, S., Cotar, A.I., Pănculescu-Gătej, I.R., Fălcuţă, E., Prioteasa, F.L., Sîrbu, A., Oprişan, G, Bădescu, D., Reiter, P. & Ceianu, C.S. (2015a) West Nile virus circulation in south-eastern Romania, 2011 to 2013. Eurosurveillance, 20 (20), pii=21130.
https://doi.org/10.2807/1560-7917.es2015.20.20.21130
Dinu, S., Pănculescu-Gătej, I.R., Florescu, S.A., Popescu, C.P., Sîrbu, A., Oprişan, G., Bădescu, D., Franco, L. & Ceianu, C.S. (2015b) Molecular epidemiology of dengue fever cases imported into Romania between 2008 and 2013. Travel Medicine and Infectious Disease, 13, 69–73.
https://doi.org/10.1016/j.tmaid.2014.11.001
Edwards, F.W. (1921) A revision of the mosquitoes of the Palearctic Region. Bulletin of Entomological Research, 12, 263–351.
https://doi.org/10.1017/s0007485300040207
European Centre for Disease Prevention and Control (2010) West Nile virus infection outbreak in humans in Romania, 2010. Available from: https://ecdc.europa.eu/sites/portal/files/media/en/publications/Publications/1104_MIR_West_Nile_outbreak_Romania.pdf (accessed 25 October 2018)
European Centre for Disease Prevention and Control (2018a) Native mosquitoes: Distribution maps. Available from: https://ecdc.europa.eu/en/disease-vectors/surveillance-and-disease-data/mosquito-maps (accessed 25 October 2018)
European Centre for Disease Prevention and Control. (2018b) West Nile fever in Europe in 2018 - human cases compared to previous seasons. Available from: https://ecdc.europa.eu/sites/portal/files/images/ECDC_WNF_Affected_current_and_past_seasons_8.jpg (accessed 25 October 2018)
Fălcuţă, E., Prioteasa, F.L. & Nicolescu, G. (2008) Investigations of the anopheline (Diptera: Culicidae) fauna from three areas belonging to the Danube Delta Biosphere Reserve in order to evaluate the risk of malaria re-emergence. Scientific Annals of the Danube Delta Institute, 14, 15–20.
Fălcuţă, E., Prioteasa, F.L., Teodorescu, I. & Nicolescu, G. (2011) Anopheles maculipennis complex (Diptera: Culicidae) in Comana area (Giurgiu County, Romania). Romanian Journal of Biology-Zoology, 56, 49–60.
Fălcuţă, E., Toty, C., Prioteasa, F.L., Nicolescu, G., Teodorescu, I., Purcărea-Ciulacu, V. & Fontenille, D. (2010) Blood-meal preferences for Anopheles maculipennis (Diptera: Culicidae) complex species in Comana, Giurgiu County (Romania). Romanian Journal of Biology-Zoology, 55, 49–55.
Florescu, S.A., Cotar, A.I., Popescu, C.P., Ceianu, C.S., Zaharia, M., Vancea, G., Codreanu, D., Badescu, D. & Ceausuet, E. (2017) First two imported cases of Zika virus infections in Romania. Vector-Borne and Zoonotic Diseases, 17, 354–357.
https://doi.org/10.1089/vbz.2016.2076
Gaffigan, T.V., Wilkerson, R.C., Pecor, J.E., Stoffer, J.A. & Anderson, T. (2015) Systematic catalog of Culicidae. Available from: http://www.mosquitocatalog.org/ (accessed 25 October 2018)
Genchi, C., Rinaldi, L., Mortarino, M., Genchi, M. & Cringoli, G. (2009) Climate and Dirofilaria infection in Europe. Veterinary Parasitology, 163, 286–292.
https://doi.org/10.1016/j.vetpar.2009.03.026
Giurcă, I. (1984) The Aedes mosquitoes (Diptera: Culicidae) in the east of Romanian plain – distribution and biology. Archives Roumaines de Pathologie experimentales et de Microbiologie, 43, 103–111.
Giurcă, I., Velehorschi, N. & Nicolescu, G. (1983) The mosquitoes in the forest of Cernica—ecological data. Archives Roumaines de Pathologie experimentales et de Microbiologie, 42, 353–363.
Gornostaeva, R.M. (2000) A revised checklist of the mosquitoes (Diptera: Culicidae) of European Russia. European Mosquito Bulletin, 6, 15–19.
Grandadam, M., Caro, V., Plumet, S., Thiberge, J.M., Souarès, Y., Failloux, A.B., Tolou, H.J., Budelot, M., Cosserat, D., Leparc-Goffart, I. & Desprès, P. (2011) Chikungunya virus, southeastern France. Emerging Infectious Diseases, 17, 910–913.
https://doi.org/10.3201/eid1705.101873
Heitmann, A., Jansen, S., Lühken, R., Helms, M., Pluskota, B., Becker, N., Kuhn, C., Schmidt-Chanasit, J. & Tannich, E. (2018) Experimental risk assessment for chikungunya virus transmission based on vector competence, distribution and temperature suitability in Europe, 2018. Eurosurveillance, 23(29), pii=1800033.
https://doi.org/10.2807/1560-7917.ES.2018.23.29.1800033
Hubálek, Z. & Halouzka, J. (1999) West Nile fever―a reemerging mosquito-borne viral disease in Europe. Emerging Infectious Diseases, 5, 643–650.
https://doi.org/10.3201/eid0505.990505
Ionică, A.M., Matei, I.A., D’Amico, G., Daskalaki, A.A., Juránková, J., Ionescu, D.T., Mihalca, A.D., Modrý, D. & Gherman, C.M. (2016) Role of golden jackals (Canis aureus) as natural reservoirs of Dirofilaria spp. in Romania. Parasites & Vectors, 9, 240.
https://doi.org/10.1186/s13071-016-1524-3
Ionică, A.M., Matei, I.A., Mircean, V., Dumitrache, M.O., D’Amico, G., Györke, A., Pantchev, N., Annoscia, G., Albrechtová, K., Otranto, D., Modrý, D. & Mihalca, A.D. (2015) Current surveys on the prevalence and distribution of Dirofilaria spp. and Acanthocheilonema reconditum infections in dogs in Romania. Parasitology Research, 114, 975–982.
https://doi.org/10.1007/s00436-014-4263-4
Ionică, A.M., Zittra, C., Wimmer, V., Leitner, N., Votýpka, J., Modrý, D., Mihalca, A.D. & Fuehrer, H.P. (2017) Mosquitoes in the Danube Delta: searching for vectors of filarioid helminths and avian malaria. Parasites & Vectors, 10, 324.
https://doi.org/10.1186/s13071-017-2264-8
Ivanescu, M.L., Acatrinei, D., Pavel, I., Sulesco, T. & Miron, L. (2015) PCR identification of five species from the Anopheles maculipennis complex (Diptera: Culicidae [sic]) in North-Eastern Romania. Acta Parasitologica, 60, 283–289.
https://doi.org/10.1515/ap-2015-0040
Javelle, E., Florescu, S.A., Asgeirsson, H., Jmor, S., Eperon, G., Leshem, E., Blum, J., Molina, I., Field, V., Pietroski, N., Eldin, C., Johnston, V., Cotar, I.A., Popescu, C., Hamer, D.H. & Gautret, P. (2019) Increased risk of chikungunya infection in travellers to Thailand during ongoing outbreak in tourist areas: cases imported to Europe and the Middle East, early 2019. Eurosurveillance, 24 (10), pii=1900146.
https://doi.org/10.2807/1560-7917.ES.2019.24.10.1900146
Kampen, H., Medlock, J.M., Vaux, A.G., Koenraadt, C.J., van Vliet, A.J., Bartumeus, F., Oltra, A., Sousa, C., Chouin, S. & Werner, D. (2015) Approaches to passive mosquito surveillance in the EU. Parasites & Vectors, 8, 9.
https://doi.org/10.1186/s13071-014-0604-5
Lupaşco, G. & Duport, M. (1968) Modifications dela faune anophelienne consecutives aux differentes mesures instituees. Archives Roumaines de Pathologie experimentales et de Microbiologie, 27, 697–706.
Lutomiah, J., Bast, J., Clark, J., Richardson, J., Yalwala, S., Oullo, D., Mutisya, J., Mulwa, F., Musila, L., Khamadi, S., Schnabel, D., Wurapa, E. & Sang, R. (2013) Abundance, diversity, and distribution of mosquito vectors in selected ecological regions of Kenya: public health implications. Journal of Vector Ecology, 38, 134–142.
https://doi.org/10.1111/j.1948-7134.2013.12019.x
Martinez-de la Puente, J., Ruiz, S., Soriguer, R. & Figuerola, J. (2013) Effect of blood meal digestion and DNA extraction protocol on the success of blood meal source determination in the malaria vector Anopheles atroparvus. Malaria Journal, 12, 109.
https://doi.org/10.1186/1475-2875-12-109
Martini, E. & Zotta, G. (1934) Races d’A. maculipennis en Roumanie. Archives Roumaines de Pathologie experimentales et de Microbiologie, 7, 135–205.
Mircean, V., Dumitrache, M.O., Györke, A., Pantchev, N., Jodies, R., Mihalca, A.D. & Cozma, V. (2012) Seroprevalence and geographic distribution of Dirofilaria immitis and tick-borne infections (Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, and Ehrlichia canis) in dogs from Romania. Vector-Borne and Zoonotic Diseases, 12, 595–603.
https://doi.org/10.1089/vbz.2011.0915
Neghina, R., Neghina, A.M., Marincu, I. & Iacobiciu, I. (2011) Malaria and the campaigns toward its eradication in Romania, 1923–1963. Vector-Borne and Zoonotic Diseases, 11, 103–110.
https://doi.org/10.1089/vbz.2009.0071
Nicolescu, G. (1981) The action of two formulations containing Bacillus thuringiensis (serotype h-14) on some autochtonous mosquito species (Diptera: Culicidae). Archives Roumaines de Pathologie experimentale et de Microbiologie, 41, 67–72.
Nicolescu, G. (1995) The mosquitoes (Diptera: Culicidae) from Romania: an annotated checklist and bibliography. Romanian Archives of Microbiology and Immunology, 54, 75–109.
Nicolescu, G. & Velehorschi, N. (1988) New records of mosquito species (Diptera: Culicidae) in Romania. Archives Roumaines de Pathologie experimentales et de Microbiologie, 47, 43–47.
Nicolescu, G., Linton, Y.-M., Vladimirescu, A., Howard, T.M. & Harbach, R.E. (2004) Mosquitoes of the Anopheles maculipennis group (Diptera: Culicidae) in Romania, with the discovery and formal recognition of a new species based on molecular and morphological evidence. Bulletin of Entomological Research, 94, 525–535.
https://doi.org/10.1079/ber2004330
Nicolescu, G., Vladimirescu, A. & Ciolpan, O. (2002) The distribution of mosquitoes in Romania (Diptera: Culicidae). Part I: Anopheles, Aedes and Culex. European Mosquito Bulletin, 13, 17–26.
Nicolescu, G., Vladimirescu, A. & Ciolpan, O. (2003a) The distribution of mosquitoes in Romania (Diptera: Culicidae). Part II: Culiseta, Coquillettidia, Ochlerotatus, Orthopodomyia and Uranotaenia. European Mosquito Bulletin, 14, 1–15.
Nicolescu, G., Vladimirescu, A. & Ciolpan, O. (2003b) The distribution of mosquitoes in Romania (Diptera: Culicidae). Part III: Detailed maps for Anopheles, Aedes and Culex. European Mosquito Bulletin, 14, 25–31.
Popescu, C.P., Florescu, S.A., Cotar, A.I., Badescu, D., Ceianu, C.S., Zaharia, M., Tardei, G., Codreanu, D., Ceausu, E. & Ruta, S.M. (2018) Re-emergence of severe West Nile virus neuroinvasive disease in humans in Romania, 2012 to 2017—implications for travel medicine. Travel Medicine and Infectious Disease, 22, 30–35.
https://doi.org/10.1016/j.tmaid.2018.03.001
Popovici, F., Sarbu, A., Nicolae, O., Pistol, A., Cucuiu, R., Stolica, B., Furtunescu, F., Manuc, M. & Popa, M.I. (2008) West Nile fever in a patient in Romania, August 2008: case report. Eurosurveillance, 13 (39), pii=18989.
Prioteasa, F.L. & Fălcuţă, E. (2010) An annotated checklist of the mosquitoes (Diptera: Culicidae) of the Danube Delta Biosphere Reserve. European Mosquito Bulletin, 28, 240–245.
Prioteasa, F.L., Dinu, S., Fălcuţă, E. & Ceianu, C.S. (2015) Established population of the invasive mosquito species Aedes albopictus in Romania, 2012–14. Journal of American Mosquito Control Association, 31, 177–181.
https://doi.org/10.2987/14-6462R
Prioteasa, F.L., Fălcuță, E., Marinov, E.M. & Nicolescu, G. (2013) Mosquitoes (Diptera: Culicidae) in Mila 26 – Maliuc area (Danube Delta, Romania) – preliminary data. Scientific Annals of Danube Delta Institute, 13, 89–96.
Prioteasa, F.L., Fălcuţă, E., Nicolescu, G., Teodorescu, I. & Reiter, P. (2010) Confirmation of the presence of Ochlerotatus zammitii (Diptera: Culicidae) in Romania. Romanian Journal of Biology-Zoology, 55, 43–48.
https://doi.org/10.2987/14-6462r
Savage, H.M., Aggarwal, D., Apperson, C.S., Katholi, C.R., Gordon, E., Hassan, H.K., Anderson, M., Charnetzky, D., McMillen, L., Unnasch, E.A. & Unnasch, T.R. (2007) Host choice and West Nile virus infection rates in blood-fed mosquitoes, including members of the Culex pipiens complex, from Memphis and Shelby County. Vector-Borne and Zoonotic Diseases, 7, 365–386.
https://doi.org/10.1089/vbz.2006.0602
Săndulesco, M. & Marotinesco, S. (1957) Determination des femelles d’Anopheles sacharovi (elutus) et des varietes du groupe Anopheles maculipennis, par l’identification des ovipositions obtenues par dissection. Archives Roumaines de Pathologie experimentales et de Microbiologie, 16, 144–149.
Sîrbu, A., Ceianu, C.S., Panculescu-Gatej, R.I., Vázquez, A., Tenorio, A., Rebreanu, R., Niedrig, M., Nicolescu, G. & Pistol, A. (2011) Outbreak of West Nile virus infection in humans, Romania, July to October 2010. Eurosurveillance, 16 (2), pii=19762.
Török, E. (2017) Investigate on the mosquito (Diptera, Culicidae) biodiversity and their pathogens in the metropolis Cluj-Napoca, Romania. European Network of Neglected Vectors and Vector-Borne Infections. Available from: http://www.eurnegvec.org/downloads/stsm_reports/2015/08_29043_Torok%20Edina.pdf (accessed 25 November 2018)
Török, E., Tomazatos, A., Cadar, D., Horváth, C., Keresztes, L., Jansen, S., Becker, N., Kaiser, A., Popescu, O., Schmidt-Chanasit, J., Jöst, H. & Lühken, R. (2016) Pilot longitudinal mosquito surveillance study in the Danube Delta Biosphere Reserve and the first reports of Anopheles algeriensis Theobald, 1903 and Aedes hungaricus Mihályi, 1955 for Romania. Parasites & Vectors, 9, 196.
https://doi.org/10.1186/s13071-016-1484-7
Tsai, T.F., Popovici, F., Cernescu, C., Campbell, G.L. & Nedelcu, N.I. (1998) West Nile encephalitis epidemic in southeastern Romania. Lancet, 352, 767–771.
https://doi.org/10.1016/s0140-6736(98)03538-7
Ungureanu, E.M. (1940a) Larves de moustiques capturees dans les forets du nord de la Roumanie (Dep. De Dorohoi). Revue Medico-Chirurgicale, 51, 147–151.
Ungureanu, E.M. (1940b) Note sur Aedes (Ochlerotatus) prodotes, Dyar, dans la region du nord de la Roumanie. Revue Medico-Chirurgicala. Bulletin de la Societe des Medicines et des Naturalistes de Jassy, 51, 152–153.
Ungureanu, E.M. (1942a) Verzeichnis der culicinaen aus dem norden Rumaniens (Gegend Iassy). Annales Scientifiques de l’Universite de Iassy, 28, 282–287.
Ungureanu, E.M. (1942b) Sur la presence d’Uranotaenia unguiculata Edwards dans la region du nord de la Roumanie (Region de Iassy). Archives Roumaines de Pathologie experimentale et de Microbiologie, 12, 475–477.
Velayudhan, R. (2012) Global Strategy for dengue prevention and control, 2012–2020. Available from: https://www.who.int/denguecontrol/resources/9789241504034/en/ (accessed 25 October 2018)
Velehorschi, N., Nicolescu, G., Ceianu, C., Giurca, I. & Bîlbîe, I. (1990) The mosquitoes (Diptera: Culicidae) in Maliuc (Danube Delta) – faunistical and ecological data. Archives Roumaines de Pathologie experimentales et de Microbiologie, 49, 269–282.
World Health Organisation. (2018) Malaria. Available from: https://www.who.int/malaria/en/ (accessed December 2018)
Wilkerson, R.C., Linton, Y.-M., Fonseca, D.M., Schultz, T.R., Price, D.C. & Strickman, D.A. (2015) Making mosquito taxonomy useful: A stable classification of tribe Aedini that balances utility with current knowledge of evolutionary relationships. Plos ONE, 10 (7), e0133602.
https://doi.org/10.1371/journal.pone.0133602
Zotta, G. (1932) Etudes sur le paludisme dans le Delta du Danube (I. Anophelisme sans paludisme). Archives Roumaines de Pathologie experimentales et de Microbiologie, 5, 133–176.
Zotta, G. (1938) Contribution a l’etude de la distribution des races d’Anopheles maculipennis en rapport avec les grandes lignes de repartition du paludisme en Roumanie. Archives Roumaines de Pathologie experimentales et de Microbiologie, 11, 209–246.
Zotta, G., Georgesco, M., Jonesco, V., Lupasco, G., Mardare, I. & Teodoresco, A.M. (1940) Nouvelle carte de distribution des races d’Anopheles maculipennis en Roumanie. Bulletin de la Section Scientifique de l’Academie Roumaine, 23, 73–87.
Zotta, G., Radaicovici, E., Teodorescu, A.M., Duport, M. & Săndulescu, M. (1943) Endemia paludică în județul Constanța: 1934–1942. Revista Științelor Medicale, 32, 819–1058.
