Abstract
In May–June 2017 an expedition on board RV ‘Investigator’ sampled benthic communities along the lower slope and abyss of eastern Australia from off Tasmania to the Coral Sea. Over 200 sabellariid specimens of the genera Phalacrostemma and Gesaia were collected during the voyage and deposited in the Australian Museum. Here we describe two new species Gesaia csiro n. sp. (4414–4436 m) and Phalacrostemma timoharai n. sp. (1013–1093 m). We did not formally describe another species of Phalacrostemma due to poor condition of the single specimen. Gesaia csiro n. sp. is the first record of the genus from Australian waters (only a planktonic larva attributed to the genus has previously been recorded), and it can be distinguished from other congeners by the smooth surface of inner paleae, distal thecae of outer paleae with long, irregular and expanded distal fringe and circled distal margin. Phalacrostemma timoharai n. sp. differs from congeners by the following combination of characters: presence of the buccal flap, absence of tentacular filament, 18–22 pairs of outer paleae, two pairs of neuropodial cirri on first thoracic segment, and only one pair of lateral lobes on second thoracic segment. Morphological descriptions are accompanied by mitochondrial cytochrome c oxidase subunit 1 (COI) and ribosomal (16S, 18S and 28S) sequence data. A key to all Australian species of sabellariids is given.
References
Altschul, S.F., Gish, W., Miller, W., Myers, E.W. & Lipman, D.J. (1990) Basic Local Alignment Search Tool. Journal of Molecular Biology, 215 (3), 403–410.
https://doi.org/10.1016/S0022-2836(05)80360-2
Augener, H. (1906) Reports on the results of dredging, under the supervision of Alexander Agassiz, in the Gulf of Mexico and the Caribbean Sea, and on the east coast of the United States, 1877–1880, by the U.S.S. Coast Survey Steamer Blake. Westindische Polychaeten. Bulletin of Museum of Comparative Zoology, Harvard, 43, 91–196.
Barrios, L.M., Chambers, S.J., Ismail, N., Guzman, H.M. & Mair, J.M. (2009) Distribution of Idanthyrsus cretus (Polychaeta: Sabellariidae) in the Tropical Eastern Pacific and application of PCR-RAPD for population analysis. Zoosymposia, 2, 487–503.
https://doi.org/10.11646/zoosymposia.2.1.34
Blank, M. & Bastrop, R. (2009) Phylogeny of the mud worm genus Marenzelleria (Polychaeta, Spionidae) inferred from mitochondrial DNA sequences. Zoologica Scripta, 38 (3), 313–321.
https://doi.org/10.1111/j.1463-6409.2008.00370.x
Caline, B., Gruet, Y. & Legendre, C. (1992) The sabellariid reefs in the Bay of Mont Saint-Michel, France. Ecology, geomorphology, sedimentology, and geologic implications. Contributions to Marine Science, Florida, 1 (1), 1–156.
Capa, M., Faroni-Perez, L. & Hutchings, P. (2015) Sabellariidae from Lizard Island, Great Barrier Reef, including a new species of Lygdamis and notes on external morphology of the median organ. Zootaxa, 4019 (1), 184–206.
https://doi.org/10.11646/zootaxa.4019.1.10
Capa, M. & Hutchings, P. (2019) Sabellariidae Johnston, 1865. In: Westheide, W., Purschke, G. & Böggemann, M. (Eds.), Handbook of Zoology. Annelida Volume 2: Pleistoannelida, Sedentaria II. De Gruyter, Berlin, pp.144–163.
https://doi.org/10.1515/9783110291681-005
Capa, M., Hutchings, P. & Peart, R. (2012) Systematic revision of Sabellariidae (Polychaeta) and their relationships with other polychaetes using morphological and DNA sequence data. Zoological Journal of the Linnean Society, 164, 245–284.
https://doi.org/10.1111/j.1096-3642.2011.00767.x
Capa, M., Hutchings, P., Aguado, M.T. & Bott, N. (2011) Phylogeny of Sabellidae (Annelida) and relationships with related taxa inferred from morphology and multiple genes. Cladistics, 27, 449–469.
https://doi.org/10.1111/j.1096-0031.2010.00341.x
Carr, C.M., Hardy, S.M., Brown, T.M., Macdonald, T.A. & Hebert, P.D.N. (2011) A Tri-Oceanic perspective: DNA barcoding reveals geographic structure and cryptic diversity in Canadian polychaetes. Plos One, 6 (7), e22232.
https://doi.org/10.1371/journal.pone.0022232
Caullery, M. (1913) Sur le genre Pallasia Qfg. et la region prostomiale des Sabellariens. Bulletin de la Société Zoologique de France, 38, 198–203.
Faroni-Perez, L., Helm, C., Burghardt, I., Hutchings, P. & Capa, M. (2016) Anterior sensory organs in Sabellariidae (Annelida). Invertebrate Biology, 135 (4), 423–447.
https://doi.org/10.1111/ivb.12153
Fauchald, K. (1977) The polychaete worms: Definitions and keys to the orders, families and genera. Natural History Museum of Los Angeles County, Science Series, 28, 1–188.
Fauvel, P. (1911) Troisième note préliminaire sur les polychètes provenant des campagnes de l’Hirondelle et de la Princesse-Alice, ou déposées dans la Musée Océanographique de Monaco. Bulletin de l’Institute Océanographique, 194, 1–41.
Gunton, L.M., Kupriyanova, E., Alvestad, T., Avery, L., Blake, J.A., Biriukova, O., Böggemann, M., Borisova, P., Budaeva, N., Burghardt, I., Capa, M., Georgieva, M.N., Glasby, C.J., Hsueh, P.–W., Hutchings, P., Jimi, N., Konsgrud, J.A., Langeneck, J. Meißner, K., Murray, A., Nikolic, M., Paxton, H., Ramos, D., Schulze, A., Sobczyk, R, Watson, C, Wiklund, H., Wilson, R., Zhadan, A., & Zhang, J.(submitted). Annelids of the eastern Australian abyss collected by the 2017 RV Investigator voyage. Marine & Freshwater Research.
Hardy, S.M., Carr, C.M., Hardman, M., Steinke, D., Corstorphine, E. & Mah, C. (2011) Biodiversity and phylogeography of Arctic marine fauna: insights from molecular tools. Marine Biodiversity, 41, 195–210.
https://doi.org/10.1007/s12526-010-0056-x
Hartman, O. (1944) Polychaetous annelids. Part VI. Paraonidae, Magelonidae, Longosomidae, Ctenodrilidae, and Sabellariidae. Allan Hancock Pacific Expeditions, 10 (3), 311–389.
Haswell, W.A. (1883) On some new Australian tubicolous annelids. Proceedings of the Linnean Society of New South Wales, 7 (4), 633–638.
https://doi.org/10.5962/bhl.part.22769
Hatschek, B. (1893) System der Anneliden, ein vorläufiger Bericht. Lotos, Naturwissenschaftliche Zeitschrift, 13, 123–126.
Helm, C., Bok, M., Hutchings, P., Kupriyanova, E. & Capa, M. (2018) Developmental studies in Sabellariidae (Annelida) reveal fate and putative settlement role of the nuchal organ. BMC Evolutionary Biology, 18 (1), 149–162.
https://doi.org/10.1186/s12862-018-1263-5
Hutchings, P., Capa, M. & Peart, R. (2012) Revision of the Australian Sabellariidae (Polychaeta) and description of eight new species. Zootaxa, 3306 (1), 1–60.
https://doi.org/10.11646/zootaxa.3306.1.1
Johansson, K.E. (1927) Beiträge zur Kenntnis des Polychaeten-Familien Hermellidae, Sabellidae und Serpulidae. Zoologiska Bidrag från Uppsala, 11, 1–184.
Johnston, G. (1865) A catalogue of the British Non-Parasitical Worms in the Collection of the British Museum. British Museum, London, 366 pp.
Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment Software Version 7: improvements in performance and usability. Molecular Biology and Evolution, 30 (4), 772–780.
https://doi.org/10.1093/molbev/mst010
Kearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., Buxton, S., Cooper, A., Markowitz, S., Duran, C., Thierer, T., Ashton, B., Meintjes, P. & Drummond, A. (2012) Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics, 28 (12), 1647–1649.
https://doi.org/10.1093/bioinformatics/bts199
Kieselbach, D. & Hausen, H. (2008) Chaetal arrangement provides no support for a close relationship of Sabellidae and Sabellariidae (Annelida). Journal of Morphology, 269, 104–117.
https://doi.org/10.1002/jmor.10537
Kinberg, J.G.H. (1867) Annulata nova. Öfversigt af Königlich Vetenskapsakademiens förhandlingar, Stockholm, 23 (9), 337–357.
Kirtley, D.W. (1994) A review and Taxonomic Revision of the Family Sabellariidae Johnston, 1865 (Annelida; Polychaeta). Sabecon Press Science Series, Vero Beach, Florida, 223 pp.
Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. (2018) MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution, 35, 1547–1549.
https://doi.org/10.1093/molbev/msy096
Kupriyanova, E.K., Macdonald, T.A. & Rouse, G.W. (2006) Phylogenetic relationships within Serpulidae (Sabellida, Annelida) inferred from molecular and morphological data. Zoologica Scripta, 35 (5), 421–439.
https://doi.org/10.1111/j.1463-6409.2006.00244.x
Lanfear, R., Calcott, B., Ho, S.Y.W. & Guindon, S. (2012) PartitionFinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Molecular Biology and Evolution, 29 (6), 1695–1701.
https://doi.org/10.1093/molbev/mss020
Lechapt, J.-P. & Kirtley, D.W. (1998) New species of bathyal and abyssal Sabellariidae (Annelida: Polychaeta) from near New Caledonia (southwest Pacific Ocean). Proceedings of the Biological Society of Washington, 111 (4), 807–822.
Lehrke, J., ten Hove, H.A., MacDonald, T.A., Bartolomaeus, T. & Bleidorn, C. (2007) Phylogenetic relationships of Serpulidae (Annelida: Polychaeta) based on 18S rDNA sequence data, and implications for opercular evolution. Organisms Diversity and Evolution, 7 (3), 195–206.
https://doi.org/10.1016/j.ode.2006.06.004
Levinsen, G.M.R. (1883) Systematisk-geografisk Oversigt over de nordiske Annulata, Gephyrea, Chaetognathi og Balanoglossi. Videnskabelige Meddelelser fra Dansk naturhistorisk Forening i Köbenhavn, 1882, 160–251.
https://doi.org/10.5962/bhl.title.16117
Lobo, J., Teixeira, M.A., Borges, L.M., Ferreira, M.S., Hollatz, C., Gomes, P. & Costa, F.O. (2016) Starting a DNA barcode reference library for shallow water polychaetes from the southern European Atlantic coast. Molecular Ecology Resources, 16 (1), 298–313.
https://doi.org/10.1111/1755-0998.12441
Marenzeller, E. von (1895) Phalacrostemme cidariophilum, eine neue Gattung und Art der Hermelliden. Anzeiger, Akademie der Wissenschaften, Wien, 32, 191–192.
McCarthy, D.A., Kramer, P. & Price, J.R. (2008) The ecological importance of a recently discovered intertidal sabellariid reef in St. Croix, U.S. Virgin Islands. Caribbean Journal of Science, 44 (2), 223–227.
https://doi.org/10.18475/cjos.v44i2.a10
McIntosh, W.C. (1885) Report on the Annelida Polychaeta collected by H.M.S. Challenger during the years 1873–1876. Report on the Scientific Results of the Voyage of H.M.S. Challenger during the years 1873–76. Zoology, 12 (Part 34), 1–554.
Moore, J.P. (1906) Additional new species of Polychaeta from the North Pacific. Proceedings of the Academy of Natural Sciences, Philadelphia, 1906, 217–260.
Nei, M. & Kumar, S. (2000) Molecular Evolution and Phylogenetics. Oxford University Press, New York, 333 pp.
Nóren, M. & Jordelius, U. (1999) Phylogeny of Prolecithophora (Platyhelminthes) inferred from18S rDNA sequences. Cladistics, 15, 103–112.
https://doi.org/10.1111/j.1096-0031.1999.tb00252.x
O’Hara, T. (2017) ‘IN2017_V03 Voyage summary’. Available from: https://www.cmar.csiro.au/data/trawler/survey_details.cfm?survey=IN2017%5FV03 (accessed 23 June 2020)
Osborn, K.J., Rouse, G.W., Goffredi, S.K. & Robison, B.H. (2007) Description and relationships of Chaetopterus pugaporcinus, an unusual pelagic polychaete (Annelida, Chaetopteridae). The Biological Bulletin, 212, 40–54.
https://doi.org/10.2307/25066579
Palumbi, S., Martin, A., Romano, S., McMillan, W.O., Stice, L. & Grabowski, G. (1991) The Simple Fool’s Guide to PCR. Version II. University of Hawaii, Honolulu, 15 pp.
Read, G. & Fauchald, K. (Ed.) (2019). World Polychaeta database. Gesaia Kirtley, 1994. Accessed through: World Register of Marine Species. Available from: http://www.marinespecies.org/aphia.php?p=taxdetails&id=325236 (accessed 7 October 219)
Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61 (3), 539–542.
https://doi.org/10.1093/sysbio/sys029
Rouse, G.W. & Pleijel, F. (2001) Polychaetes. Oxford University Press, London, xiii + 354 pp.
Rousset, V., Rouse, G.W., Siddall, M.E., Tillier, A. & Pleijel, F. (2004) The phylogenetic position of Siboglinidae (Annelida) inferred from 18S rRNA, 28S rRNA and morphological data. Cladistics, 20 (6), 518–533.
https://doi.org/10.1111/j.1096-0031.2004.00039.x
Rousset, V., Pleijel, F., Rouse, G.W., Erseus, C. & Siddall, M.E. (2007) A molecular phylogeny of annelids. Cladistics, 23 (1), 41–63.
https://doi.org/10.1111/j.1096-0031.2006.00128.x
Savigny, J.C. (1822) Système des annelides, principalement de celles des côtes de l’Égypte et de la Syrie, offrant les caractères tant distinctifs que naturels des Ordres, Familles et Genres, avec la description des espèces. Description de l’Egypte. Histoire Naturelle, Paris, 1, 1–128.
Struck, T.H., Halanych, K.M. & Purschke, G. (2005) Dinophilidae (Annelida) is most likely not a progenetic Eunicida: evidence from 18S and 28S rDNA. Molecular Phylogenetics and Evolution, 37 (2), 619–623.
https://doi.org/10.1016/j.ympev.2005.07.010
Struck, T.H., Nesnidal, M.P., Purschke, G. & Halanych, K.M. (2008) Detecting possibly saturated positions in 18S and 28S sequences and their influence on phylogenetic reconstruction of Annelida (Lophotrochozoa). Molecular Phylogenetics and Evolution, 48 (2), 628–645.
https://doi.org/10.1016/j.ympev.2008.05.015
Struck, T.H. (2019) Phylogeny. In: Westheide, W., Purschke, G. & Böggemann, M. (Eds.), Handbook of Zoology. Annelida. Vol. 1. Annelida Basal Groups and Pleistoannelida, Sedentaria I. De Gruyter, Berlin, pp. 37–68.
https://doi.org/10.1515/9783110291582-002
Treadwell, A.L. (1926) Contributions to the biology of the Philippine Archipelago and adjacent regions. Additions to the polychaetous annelids collected by the United States Fisheries steamer 'Albatross', 1907–1910, including one new genus and three new species. Bulletin of the United States National Museum, 100, 183–193.
Zanol, J., Halanych, K.M., Struck, T.H. & Fauchald, K. (2011) Phylogeny of the bristle worm family Eunicidae (Eunicida, Annelida) and the phylogenetic utility of noncongruent 16S, COI and 18S in combined analyses. Molecular Phylogenetics and Evolution, 55, 660–676.
https://doi.org/10.1016/j.ympev.2009.12.024
Zrzavý, J., Říha, P., Piálek, L. & Janouškovec, J. (2009) Phylogeny of Annelida (Lophotrochozoa): total-evidence analysis of morphology and six genes. BMC Evolutionary Biology, 9, 189.
https://doi.org/10.1186/1471-2148-9-189