Abstract
Paracobitis salihae, new species, from the Göksu River in the western upper Euphrates drainage, is distinguished from other Paracobitis species by possessing a truncate caudal-fin, and a dark-brown vermiculate or marbled colour pattern. It is also characterised by 19 variable nucleotide substitutions, three diagnostic nucleotides and 3.6% minimum K2P distance compared to geographically adjacent and related P. zabgawraensis. Paracobitis salihae might be Critically Endangered and is threatened by dam constructions.
References
Bânârescu, P. & Nalbant, T.T. (1964) Süsswasserfische der Türkei. 2. Teil. Cobitidae. Mitteilungen aus dem Hamburgischen Zoologischen Museum und Institut, 61, 159–201.
Erk’akan, F., Özeren, S.C. & Nalbant, T.T. (2008) Two new species of stone loaches from Turkey (Teleostei: Nemacheilidae). Journal of Fisheries International, 3, 115–119.
Esmaeili, H.R., Mousavi-Sabet, H., Sayyadzadeh, G., Vatandoust, S. & Freyhof, J. (2014) Paracobitis atrakensis, a new crested loach from northeast Iran (Teleostei: Nemacheiliidae). Ichthyological Exploration of Freshwaters, 25, 237–242.
Freyhof, J., Esmaeili, H.R., Sayyadzadeh, G. & Geiger, M. (2014) Review of the crested loaches of the genus Paracobitis from Iran and Iraq with the description of four new species (Teleostei: Nemacheilidae). Ichthyological Exploration of Freshwaters, 25 (1), 11–38.
Freyhof, J., Kaya, C., Turan, D. & Geiger, M.F. (2019) Review of the Oxynoemacheilus tigris group with the description of two new species from the Euphrates drainage (Teleostei: Nemacheilidae). Zootaxa, 4612 (1), 29–57.
https://doi.org/10.11646/zootaxa.4612.1.2
Geldiay, R. & Balık, S. (2007) Türkiye Tatlı Su Balıkları. Ege Üniversitesi Su Ürünleri Fakültesi yayınları, 166 (6), 1–519. [in Turkish, ISBN: 978-975-483-731-5]
Hall, T.A. (1999) BioEdit: a user–friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.
Hashemzadeh Segherloo, I.H., Ghaedrahmati, N. & Freyhof, J. (2016) Eidinemacheilus, a new generic name for Noemacheilus smithi Greenwood (Teleostei; Nemacheilidae). Zootaxa, 4147 (4), 466–476.
Kaya, C., Turan, D. & Ünlü, E. (2016) The latest status and distribution of fishes in upper Tigris River and two new records for Turkish freshwaters. Turkish Journal of Fisheries and Aquatic Sciences, 16, 545–562.
https://doi.org/10.4194/1303-2712-v16_3_07
Kimura, M. (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16 (2), 111–120.
https://doi.org/10.1007/BF01731581
Kottelat, M. & Freyhof, J. (2007) Handbook of European freshwater fishes. Kottelat, Cornol & Freyhof, Berlin, xiv + 646 pp.
Kumar, S., Stecher, G., Li, M., Kınyaz, C. & Tamura, K. (2018) MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution, 35, 1547–1549.
https://doi.org/10.1093/molbev/msy096
Kuru, M. (1975) Dicle-Fırat, Kura-Aras, Van Gölü Karadeniz Havzası Tatlısularında Yaþayan Balıkların (Pisces) Sistematik ve Zoocoğrafik Yönden İncelenmesi. Doçentlik Tezi. Atatürk Üniversitesi, Fen Fakültesi, Erzurum, 180 pp. [in Turkish]
Mousavi-Sabet, H., Sayyadzadeh, G., Esmaeili, H.R., Eagderi, S., Patimar, R. & Freyhof, J. (2015) Paracobitis hircanica, a new crested loach from the southern Caspian Sea basin (Teleostei: Nemacheilidae). Ichthyological Exploration of Freshwaters, 25, 237–242.
Mousavi-Sabet, H., Vatandoust, S., Geiger, M.F. & Freyhof, J. (2019) Paracobitis abrishamchiani, a new crested loach from the southern Caspian Sea basin (Teleostei: Nemacheilidae). Zootaxa, 4545 (3), 375–388.
https://doi.org/10.11646/zootaxa.4545.3.3
Posada, D. (2008) jModelTest: phylogenetic model averaging. Molecular Biology and Evolution, 25 (7), 1253–1256.
https://doi.org/10.1093/molbev/msn083
Sayyadzadeh, G., Esmaeili, H.R. & Eagderi, S. (2019) Taxonomic status of the loaches Paracobitis vignai and P. rhadinaea (Teleostei: Nemacheilidae) in Iran. Zoology in the Middle East, 65 (3), 221–229.
https://doi.org/10.1080/09397140.2019.1586143
Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673–4680.
https://doi.org/10.1093/nar/22.22.4673
Tamura, K. & Nei, M. (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution, 10 (3), 512–26.
https://doi.org/10.1093/oxfordjournals.molbev.a040023
Ward, R.D., Zemlak, T.S, Innes, B.H., Last, P.R. & Hebert, P.D.N. (2005) DNA barcoding Australia’s fish species. Philosophical Transactions of the Royal Society of London Series B, Biological Sciences, 360, 1847–1857.
https://doi.org/10.1098/rstb.2005.1716
Zhang, J., Kapli, P., Pavlidis, P. & Stamatakis, A. (2013) A general species delimitation method with applications to phylogenetic placements. Bioinformatics, 29 (22), 2869–2876.