Abstract
Vietnamella chebalingensis Tong, sp. nov. is described based on larval stage from China. The new species is distinguished from its congeners by the following combination of characters: body purple black with mottled white markings; length of inner pairs of cephalic projection approximately half of the outer pairs; posterior margin of abdominal tergites I–VI and VIII–X each with a pair of pointed projections, but tergite VII bearing only a single projection. The morphological evidence and molecular data (COI, Kimura 2-parameter) support that this new species represents the sixth species of the genus Vietnamella. An identification key to known larvae of the genus Vietnamella is provided.
References
Astrin, J.J. & Stüeben, P.E. (2008) Phylogeny in cryptic weevils: molecules, morphology and new genera of western Palaearctic Cryptorhynchinae (Coleoptera : Curculionidae). Invertebrate Systematics, 22 (5), 503–522.
https://doi.org/10.1071/IS07057
Auychinda, C., Jacobus, L.M., Sartori, M. & Boonsoong, B. (2020a) A new species of Vietnamella Tsherova 1972 (Ephemeroptera: Vietnamellidae) from Thailand. Insects, 11 (9), 18.
https://doi.org/10.3390/insects11090554
Auychinda, C., Sartori, M. & Boonsoong, B. (2020b) Vietnamellidae (Insecta, Ephemeroptera) of Thailand. ZooKeys, 902, 17–36.
https://doi.org/10.3897/zookeys.902.46844
Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3 (5), 294–299.
Gattolliat, J.L., Cavallo, E., Vuataz, L. & Sartori, M. (2015) DNA barcoding of Corsican mayflies (Ephemeroptera) with implications on biogeography, systematics and biodiversity. Arthropod Systematics & Phylogeny, 73 (1), 3–18.
Hu, Z., Ma, Z.X., Luo, J.Y. & Zhou, C.F. (2017) Redescription and commentary on the Chinese mayfly Vietnamella sinensis (Ephemeroptera: Vietnamellidae). Zootaxa, 4286 (3), 381–390.
https://doi.org/10.11646/zootaxa.4286.3.5
Kalyaanamoorthy, S., Bui, Q.M., Wong, T.K.F., von Haeseler, A. & Jermiin, L.S. (2017) ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods, 14 (6), 587.
https://doi.org/10.1038/NMETH.4285
Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution, 30(4), 772–780.
https://doi.org/10.1093/molbev/mst010
Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33 (7), 1870–1874.
https://doi.org/10.1093/molbev/msw054
Lanfear, R., Frandsen, P.B., Wright, A.M., Senfeld, T. & Calcott, B. (2016) PartitionFinder 2: new methods for selecting partitioned models of evolution formolecular and morphological phylogenetic analyses. Molecular Biology and Evolution, 34 (3), 772–773.
https://doi.org/10.1093/molbev/msw260
Miller, M.A., Schwartz, T., Pickett, B.E., He, S., Klem, E.B., Scheuermann, R.H., Passarotti, M., Kaufman, S. & O'Leary, M.A. (2015) A restful API for access to phylogenetic tools via the CIPRES Science Gateway. Evolutionary Bioinformatics, 11, 43–48.
https://doi.org/10.4137/EBO.S21501
Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Hohna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: Efficient bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61 (3), 539–542.
https://doi.org/10.1093/sysbio/sys029
Selvakumar, C., Sinda, B., Vasanth, M., Subramanian, K.A. & Sivaramakrishnan, K.G. (2018) A new record of monogeneric family Vietnamellidae (Insecta: Ephemeroptera) from India. Journal of Asia-Pacific Entomology, 21, 994–998.
https://doi.org/10.1016/j.aspen.2018.07.015
Talavera, G. & Castresana, J. (2007) Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments. Systematic Biology, 56 (4), 564–577.
https://doi.org/10.1080/10635150701472164
Tang, M., Tan, M., Meng, G., Yang, S., Su, X., Liu, S., Song, W., Li, Y., Wu, Q., Zhang, A. & Zhou, X. (2014) Multiplex sequencing of pooled mitochondrial genomes-a crucial step toward biodiversity analysis using mito-metagenomics. Nucleic Acids Research, 42 (22), 1–13.
https://doi.org/10.1093/nar/gku917
Tshernova, O.A. (1972) [Some new species of mayflies from Asia (Ephemeroptera, Heptageniidae, Ephemerellidae)]. Revue d’Entomologie de l’URSS, 51, 604–614. [in Russian]
Zhang, D., Gao, F., Jakovlic, I., Zou, H., Zhang, J., Li, W.X. & Wang, G.T. (2020) PhyloSuite: An integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies. Molecular Ecology Resources, 20 (1), 348–355.
https://doi.org/10.1111/1755-0998.13096