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Issue: Vol. 4890 No. 2: 2 Dec. 2020
Type: Article
Published: 2020-12-02
DOI: 10.11646/zootaxa.4890.2.6
Page range: 257–265
Abstract views: 94
PDF downloaded: 5

Description of larva and female of Polypedilum (Probolum) bullum Zhang & Wang with DNA barcodes

National Demonstration Center for Experimental Fisheries, Science Education, Shanghai Ocean University, Shanghai 201306, P. R. China.
National Demonstration Center for Experimental Fisheries, Science Education, Shanghai Ocean University, Shanghai 201306, P. R. China.
College of Life Sciences, Taizhou University, Taizhou, Zhejiang 318000, China.
College of Life Sciences, Taizhou University, Taizhou, Zhejiang 318000, China.
National Demonstration Center for Experimental Fisheries, Science Education, Shanghai Ocean University, Shanghai 201306, P. R. China.
Diptera Chironomidae Polypedilum Probolum larva female DNA barcodes

Abstract

The larva and female of Polypedilum (Probolum) bullum Zhang & Wang associated with morphological characteristics and DNA barcodes are described and illustrated for the first time. The female is characteristic with developed dorsomesal lobe and ventrolateral lobe both densely covered with apical setae, ventrolateral lobe partially covered by dorsomesal lobe. The larva is distinguished by the shape of mentum, pecten epipharyngis, labral SI and labral SII.

 

References

  1. Carew, M.E., Pettigrove, V., Cox, R.L. & Hoffmann, A.A. (2007) DNA identification of urban Tanytarsini chironomids (Diptera: Chironomidae). Journal of the North American Benthological Society, 26 (4), 587–600.

    https://doi.org/10.1899/06–120.1

    Carew, M.E., Pettigrove, V. & Hoffmann, A.A. (2005) The utility of DNA markers in classical taxonomy: using Cytochrome Oxidase I markers to differentiate Australian Cladopelma (Diptera: Chironomidae) Midges. Annals of the Entomological Society of America, 98 (4), 587–594.

    https://doi.org/10.1603/0013–8746(2005)098[0587:TUODMI]2.0.CO;2

    Cranston, P.S., Martin, J. & Spies, M. (2016) Cryptic species in the nuisance midge Polypedilum nubifer (Skuse) (Diptera: Chironomidae) and the status of Tripedilum Kieffer. Zootaxa, 4079 (4), 429–447.

    https://doi.org/10.11646/zootaxa.4079.4.3

    Ekrem, T., Willassen, E. & Stur, E. (2007) A comprehensive DNA sequence library is essential for identification with DNA barcodes. Molecular Phylogenetics and Evolution, 43 (2), 530–542.

    https://doi.org/10.1016/j.ympev.2006.11.021

    Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3 (5), 294–299.

    Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Molecular Biology and Evolution, 33 (7), 1870–1874.

    https://doi.org/10.1093/molbev/msw054

    Lin, X.-L., Yu, H.-J., Zhang, R.-L. & Wang, X.-H. (2019) Polypedilum (Cerobregma) heberti sp. n. (Diptera: Chironomidae) from Gaoligong Mountains, Yunnan, China. Zootaxa, 4571 (2), 255–262.

    https://doi.org/10.11646/zootaxa.4571.2.5

    Maschwitz, D.E. & Cook, E.F. (2000) Revision of the Nearctic species of the genus Polypedilum Kieffer (Diptera: Chironomidae) in the subgenera P. (Polypedilum) and P. (Uresipedilum) Oyewo and Sæther. Bulletin of the Ohio Biological Survey, New Series, 12, 1–135.

    Pinho, L.C. & Silva, F.L. (2020) Description of two new species of Polypedilum (Asheum) and immature stages of Polypedilum (A.) curticaudatum (Diptera: Chironomidae). Zootaxa, 4759 (2), 179–190.

    https://doi.org/10.11646/zootaxa.4759.2.2

    Sæther, O.A., Andersen, T., Pinho, L.C. & Mendes, H.F. (2010) The problems with Polypedilum Kieffer (Diptera: Chironomidae), with the description of Probolum subgen. n. Zootaxa, 2497 (1), 1–36.

    https://doi.org/10.11646/zootaxa.2497.1.1

    Sæther, O.A. (1969) Some Nearctic Podonominae, Diamesinae, and Orthocladiinae (Diptera: Chironomidae). Bulletin of the Fisheries Research Board of Canada, 170, 1–154.

    Sæther, O.A. (1980) Glossary of chironomid morphology terminology (Diptera: Chironomidae). Entomologica scandinavica Supplement, 14, 1–51.

    Shimabukuro E.M., Trivinho-Strixino S. & Lamas C.J.E. New Polypedilum Kieffer (Diptera: Chironomidae) from mountains of the Atlantic Forest, Brazil. Zootaxa, 4612 (4), 518–532.

    https://doi.org/10.11646/zootaxa.4612.4.4

    Song, C., Lin, X.-L., Wang, Q. & Wang, X.-H. (2018) DNA barcodes successfully delimit morphospecies in a superdiverse insect genus. Zoologica Scripta, 47(3), 311–324.

    https://doi.org/10.1111/zsc.12284

    Swindell, S.R. & Plasterer, T.N. (1997) SEQMAN. Contig assembly. Methods in Molecular Biology, 70 (70), 75–89.

    https://doi.org/10.1385/0–89603–358–9:75

    Helge-Friedrich, T. (2004) Analysis for free: Comparing programs for sequence analysis. Briefings in Bioinformatics, 5 (1), 82–87.

    https://doi.org/10.1093/bib/5.1.82

    Yan, C.-C., Song, C., Liu, T., Zhao, G.-J. & Hou, Z.-Y. (2017) Two new and one newly recorded species of Polypedilum Kieffer 1912 with DNA barcodes from Oriental China (Chironomidae: Diptera). Zootaxa, 4238 (1), 109–118.

    https://doi.org/10.11646/zootaxa.4238.1.8

    Yamamoto, N. & Yamamoto, M. (2015) A revised subgeneric position for Polypedilum (Probolum) simantokeleum, with description of a new Uresipedilum species in Japan (Diptera: Chironomidae). Zootaxa, 3999 (3), 439–445.

    https://doi.org/10.11646/zootaxa.3999.3.9

    Yamamoto, N., Yamamoto, M. & Hirowatari, T. (2016) Erection of a new subgenus in the genus Polypedilum Kieffer, 1912 (Diptera: Chironomidae). Japanese Journal of Systematic Entomology, 22 (2), 195–197.

    Zhang, R.-L. & Wang, X.-H. (2004) Polypedilum (Uresipedilum) Oyewo and Sæther from China (Diptera: Chironomidae). Zootaxa, 565 (1), 1–38.

    https://doi.org/10.11646/zootaxa.565.1.1