Skip to main content Skip to main navigation menu Skip to site footer
Responsive image
Issue: Vol. 4933 No. 3: 22 Feb. 2021
Type: Article
Published: 2021-02-22
DOI: 10.11646/zootaxa.4933.3.1
Page range: 301–323
Abstract views: 307
PDF downloaded: 17

Phylogenetic relationships of the poorly known treefrog Boana hobbsi (Cochran & Goin, 1970) (Anura: Hylidae), systematic implications and remarks on morphological variations and geographic distribution

Universidade Federal do Amazonas, Instituto de Ciências Biológicas, Programa de Pós-Graduação em Zoologia, Avenida General Rodrigo Otávio J. Ramos 6200, 69080-900, Manaus, Amazonas, Brazil. Universidade Federal do Amazonas, Laboratório de Evolução e Genética Animal, Departamento de Genética, Avenida General Rodrigo Otávio J. Ramos 6200, Japiim, 69080-900, Manaus, Amazonas, Brazil.
Instituto Nacional de Pesquisas da Amazônia (INPA), Coordenação de Biodiversidade, Programa de Coleções Científicas Biológicas, Avenida André Araújo, 69067-375, Petrópolis, Manaus, Amazonas, Brazil.
Universidade Federal do Amazonas, Laboratório de Evolução e Genética Animal, Departamento de Genética, Avenida General Rodrigo Otávio J. Ramos 6200, Japiim, 69080-900, Manaus, Amazonas, Brazil.
Universidade Federal do Amazonas, Laboratório de Evolução e Genética Animal, Departamento de Genética, Avenida General Rodrigo Otávio J. Ramos 6200, Japiim, 69080-900, Manaus, Amazonas, Brazil.
Universidade Federal do Amazonas, Laboratório de Evolução e Genética Animal, Departamento de Genética, Avenida General Rodrigo Otávio J. Ramos 6200, Japiim, 69080-900, Manaus, Amazonas, Brazil. Universidade Regional do Cariri, Programa de Pós-Graduação em Diversidade Biológica e Recursos Naturais, Campus do Pimenta, Rua Cel. Antônio Luiz, 1161, 63105-000, Crato, Ceará, Brazil.
Universidade Federal do Ceará, Departamento de Biologia, Centro de Ciências, Campus do Pici, Fortaleza, Ceará, Brazil
Universidade Federal do Amazonas, Laboratório de Evolução e Genética Animal, Departamento de Genética, Avenida General Rodrigo Otávio J. Ramos 6200, Japiim, 69080-900, Manaus, Amazonas, Brazil.
Instituto Nacional de Pesquisas da Amazônia (INPA), Coordenação de Biodiversidade, Programa de Coleções Científicas Biológicas, Avenida André Araújo, 69067-375, Petrópolis, Manaus, Amazonas, Brazil.
Universidade Federal do Amazonas, Instituto de Ciências Biológicas, Programa de Pós-Graduação em Zoologia, Avenida General Rodrigo Otávio J. Ramos 6200, 69080-900, Manaus, Amazonas, Brazil. Universidade Federal do Amazonas, Departamento de Biologia, Avenida General Rodrigo Otávio J. Ramos, 6200, Coroado, 69080-900, Manaus, Amazonas, Brazil.
Instituto Nacional de Pesquisas da Amazônia (INPA), Coordenação de Biodiversidade, Programa de Coleções Científicas Biológicas, Avenida André Araújo, 69067-375, Petrópolis, Manaus, Amazonas, Brazil.
Universidade Federal do Amazonas, Instituto de Ciências Biológicas, Programa de Pós-Graduação em Zoologia, Avenida General Rodrigo Otávio J. Ramos 6200, 69080-900, Manaus, Amazonas, Brazil. Universidade Federal do Amazonas, Laboratório de Evolução e Genética Animal, Departamento de Genética, Avenida General Rodrigo Otávio J. Ramos 6200, Japiim, 69080-900, Manaus, Amazonas, Brazil.
Universidade Federal do Amazonas, Instituto de Ciências Biológicas, Programa de Pós-Graduação em Zoologia, Avenida General Rodrigo Otávio J. Ramos 6200, 69080-900, Manaus, Amazonas, Brazil. Universidade Federal do Amazonas, Laboratório de Evolução e Genética Animal, Departamento de Genética, Avenida General Rodrigo Otávio J. Ramos 6200, Japiim, 69080-900, Manaus, Amazonas, Brazil.
Universidade Federal do Amazonas, Instituto de Ciências Biológicas, Programa de Pós-Graduação em Zoologia, Avenida General Rodrigo Otávio J. Ramos 6200, 69080-900, Manaus, Amazonas, Brazil. Universidade Federal do Amazonas, Departamento de Biologia, Avenida General Rodrigo Otávio J. Ramos, 6200, Coroado, 69080-900, Manaus, Amazonas, Brazil.
Amphibia Amazonia Cophomantini B. benitezi group B. punctata group Darwinian shortfall Phylogeny Wallacean shortfall

Abstract

Boana hobbsi is a poorly known hylid frog currently placed within the Boana punctata group. Yet, morphological, ecological and bioacoustic traits do not support this placement, with no molecular data being available to date to test this hypothesis. Based on newly collected mitochondrial DNA sequences, morphological data review and field observations, we provide new insight into the phylogenetic relationships, morphological variations and geographic distribution of B. hobbsi. Our findings reveal that B. hobbsi is nested (with strong support) within the Boana benitezi group, recovering once more a polyphyletic Boana punctata group. Supported by this new genetic, morphological and ecological evidence, we propose a new taxonomic arrangement which includes B. hobbsi as a member of the Boana benitezi group. Furthermore, we emphasize the importance of conducting biological inventories in remote Amazonian areas, where many taxonomic and geographic knowledge gaps persist with regards to Amphibian diversity.

 

References

  1. Acosta-Galvis, A.R. (2000) Ranas, Salamandras y Caecilias (Tetrapoda: Amphibia) de Colombia. Biota Colombiana, 1 (3), 289–319.

    Acosta-Galvis, A.R. (2018) Anfíbios del Escudo Guayanés en Colombia. In: Lasso, C.A. & Señaris, J.C. (Eds.), Volumen VI. Fauna Silvestre del Escudo Guayanés (Colombia-Venezuela). Série Editorial Fauna Silvestre Neotropical. Instituto de Investigación de Recursos Biológicos Alexander Von Humboldt, Bogotá D.C., pp. 61–97.

    Antonelli, A., Ariza, M., Albert, J., Andermann, T., Azevedo, J., Bacon, C., Faurby, S., Guedes, T., Hoorn, C., Lohmann, L.G., Matos-Maraví, P., Ritter, C.D., Sanmartín, I., Silvestro, D., Tejedor, M., ter Steege, H., Tuomisto, H., Werneck, F.P., Zizka, A. & Edwards, S.V. (2018a) Conceptual and empirical advances in Neotropical diversity research. PeerJ, 6, e5644.

    https://doi.org/10.7717/peerj.5644

    Antonelli, A., Zizka, A., Carvalho, F.A., Schran, R., Bacon, C.D., Silvestro, D. & Condamine, F.L. (2018b) Amazonia is the primary source of Neotropical biodiversity. PNAS, 115 (23), 6034–6039.

    https://doi.org/10.1073/pnas.1713819115

    Antunes, A.P, Faivovich, J. & Haddad, C.F.B. (2008) A new species from the Atlantic Forest of Southeastern Brazil (Amphibia: Anura: Hylidae). Copeia, 2008 (1), 179–190.

    https://doi.org/10.1643/CH-06-163

    Assis, L.C.S. (2018) Revisiting the Darwinian shortfall in biodiversity conservation. Biodiversity and Conservation, 27 (11), 2859–2875.

    https://doi.org/10.1007/s10531-018-1573-3

    Ávila, R.W., Pansonato, A., Perez, R., De Carvalho, V.T., Roberto, I.J., Morais, D.H., Almeida, A.P., Rojas, R., Gordo, M. & Farias, I.P. (2018) On Rhinella gildae Vaz-Silva, Maciel, Bastos & Pombal 2015 (Anura: Bufonidae): Phylogenetic relationship, morphological variation, advertisement and release calls and geographic distribution. Zootaxa, 4462 (2), 274–290.

    https://doi.org/10.11646/zootaxa.4462.2.9

    Azevedo-Ramos, C. & Galatti, U. (2002) Patterns of amphibian diversity in Brazilian Amazonia: Conservation implications. Biological Conservation, 103 (1), 103–111.

    https://doi.org/10.1016/S0006-3207(01)00129-X

    Barrio-Amorós, C.L. & Brewer-Carias, C. (2008) Herpetological results of the 2002 expedition to Sarisariñama, a tepui in Venezuelan Guayana, with the description of five new species. Zootaxa, 1942 (1), 1–68.

    https://doi.org/10.11646/zootaxa.1942.1.1

    Barrio-Amorós, C.L., Rojas-Runjaic, F.J.M. & Señaris, J.C. (2019) Catalogue of the amphibians of Venezuela: Illustrated and annotated species list, distribution, and conservation. Amphibian & Reptile Conservation, 13 (1), 1–198.

    Benson, D.A., Cavanaugh, M., Clark, K., Karsch-Mizrachi, I., Lipman, D.J., Ostell, J. & Sayers, E.W. (2013) GenBank. Nucleic Acids Research, 41, D36–D42.

    https://doi.org/10.1093/nar/gks1195

    Berneck, B.V.M., Haddad, C.F.B., Lyra, M.L., Cruz, C.A.G. & Faivovich, J. (2016) The Green Clade grows: A phylogenetic analysis of Aplastodiscus (Anura; Hylidae). Molecular Phylogenetics and Evolution, 97, 213–223.

    https://doi.org/10.1016/j.ympev.2015.11.014

    Bini, L.M., Diniz-Filho, J.A.F., Rangel, T.F.L.V., Bastos, R.P. & Pinto, M.P. (2006) Challenging Wallacean and Linnean shortfalls: knowledge gradients and conservation planning in a biodiversity hotspot. Diversity and Distribution, 12 (5), 475–482.

    https://doi.org/10.1111/j.1366-9516.2006.00286.x

    Boulenger, G.A. (1899) Descriptions of new batrachians in the collection of the British Museum (Natural History). Annals and Magazine of Natural History, Series 7, 3 (16), 273–277.

    https://doi.org/10.1080/00222939908678122

    Brito, D. (2010) Overcoming the Linnean shortfall: Data deficiency and biological surveys priorities. Basic and Applied Ecology, 11 (8), 709–713.

    https://doi.org/10.1016/j.baae.2010.09.007

    Brunetti, A.E., Hermida, G.H. & Faivovich, J. (2012) New insights into sexually dimorphic skin glands of Anurans: The structure and ultrastructure of mental and lateral glands in Hypsiboas punctatus (Amphibia: Anura: Hylidae). Journal of Morphology, 273, 1257–1271.

    https://doi.org/10.1002/jmor.20056

    Brunetti, A.E., Hermida, G.H., Luna, M.C., Barsotti, A.M.G., Jared, C., Antoniazzi, M.M., Rivera-Correa, M., Berneck, B.V.M. & Faivovich, J. (2015) Diversity and evolution of sexually dimorphic mental and lateral glands in Cophomantini treefrogs (Anura: Hylidae: Hylinae). Biological Journal of the Linnean Society, 114 (1), 12–34.

    https://doi.org/10.1111/bij.12406

    Camargo, E.G., Rivero, R. & Barrio-Amorós , C.L. (2014) Datos sobre la distribuición de cuatro espécies de anfíbios (Amphibia, Anura) de Venezuela. Anartia, 26, 63–68.

    Caminer, M.A. & Ron, S.R. (2014) Systematic of the Hypsiboas calcaratus and Hypsiboas fasciatus species complex (Anura, Hylidae) with description of four new species. Zookeys, 370 (1), 1–68.

    https://doi.org/10.3897/zookeys.370.6291

    Caminer, M.A. & Ron, S.R. (2020) Systematics of the Boana semilineata species group (Anura: Hylidae), with a description of two new species from Amazonian Ecuador. Zoological Journal of the Linnean Society, zlaa002.

    https://doi.org/10.1093/zoolinnean/zlaa002

    Caramaschi, U. & Cruz, C.A.G. (2004) Duas novas espécies de Hyla do grupo H. polytaenia Cope, 1870 do sudeste do Brasil (Amphibia, Anura, Hylidae). Arquivos do Museu Nacional do Rio de Janeiro, 62, 247–254.

    Caramaschi, U. & Velosa, A. (1996) Nova especie de Hyla Laurenti, 1768 do leste brasileiro (Amphibia, Anura, Hylidae). Boletim do Museu Nacional, Nova Série, Zoologia, 365, 1–7.

    Cardoso, P., Erwin, T.L., Borges, P.A.V. & New, T.R. (2011) The seven impediments in invertebrate conservation and how to overcome them. Biological Conservation, 144, 2647–2655.

    https://doi.org/10.1016/j.biocon.2011.07.024

    Carvalho, T.R.D., Moraes, L.J.C.L., Lima, A.P., Fouquet, A., Peloso, P.L.V., Pavan, D., Drummond, L.O., Rodrigues, M.T., Giaretta, A.A., Gordo, M., Neckel-Oliveira, S. & Haddad, C.F.B. (2020) Systematic and historical biogeography of the Neotropical foam-nesting frogs of the Adenomera heyeri clade (Leptodactylidae), with description of six new Amazonian species. Zoological Journal of Linnean Society, zlaa051.

    https://doi.org/10.1093/zoolinnean/zlaa051

    Castroviejo-Fisher, S., Köhler, J., De la Riva, I. & Padial, J.M. (2017) A new morphologically cryptic species of Phyllomedusa (Anura: Phyllomedusidae) from Amazonian forests of northern Peru revealed by DNA sequences. Zootaxa, 4269 (2), 245–264.

    https://doi.org/10.11646/zootaxa.4269.2.4

    Cochran, D.M. & Goin, C.J. (1970) Frogs of Colombia. Smithsonian Institution Press, Washington, D.C., 655 pp.

    https://doi.org/10.5479/si.03629236.288.1

    Cope, E.D. (1870) Seventh contribution to the herpetology of tropical America. Proccedings of the American Philosophical Society, 11, 147–169.

    Crump, M.L. & Scott Jr., N.J. (1994) Visual encounter survey. In: Heyer, W.R., Donnelly, M.A., McDiarmid, R.W., Hayek, L.C. & Foster, M.S. (Eds.) Measuring and monitoring biological diversity: Standard methods for amphibians. Smithsonian Institute press, Whashington, D.C., pp. 84–92.

    Darst, C.R. & Canattella, D.C. (2004) Novel relationships among hyloid frogs inferred from 12S and 16S mitochondrial DNA sequences. Molecular Phylogenetics and Evolution, 31 (2), 462–475.

    https://doi.org/10.1016/j.ympev.2003.09.003

    Diniz-Filho, J.A.F., Loyola, R.D., Raia, P., Mooers, A.O. & Bini, L.M. (2013) Darwinian shortfall in biodiversity conservation. Trends in Ecology and Evolution, 28 (12), 689–695.

    https://doi.org/10.1016/j.tree.2013.09.003

    Donnelly, M.A. & Myers, C.W. (1991) Herpetological results of the 1990 Venezuelan Expedition to the summit of Cerro Guaiquinima, with new tepui reptiles. American Museum Novitates, 3017, 1–54. [http://hdl.handle.net/2246/5042]

    Dubois, A. (2017) The nomenclatural status of Hysaplesia, Hylaplesia, Dendrobates and related nomina (Amphibia, Anura), with general comments on zoological nomenclature and its governance, as well as on taxonomic databases and websites. Bionomina, 11 (1), 1.

    https://doi.org/10.11646/bionomina.11.1.1

    Duellman, W.E. (1974) A reassessment of the taxonomic status of some neotropical hylid frogs. Occasional Papers of the Museum of Hatural History, The University of Kansas, Lawrece, Kansas, 27, 1–27.

    Duellman, W.E. & Hoogmoed, M.S. (1992) Some Hylid frogs from the Guiana Highlands, Northeastern South America: New species, distributioal records and generic reallocation. Occasional Papers of The Museum of Natural History, 147, 1–21.

    Duellman, W.E.; Marion, A.B. & Hedges, S.B. (2016) Phylogenetics, classification, and biogeography of the treefrogs (Amphibia: Anura: Arboranae). Zootaxa, 4140 (1), 1–109.

    https://doi.org/10.11646/zootaxa.4104.1.1

    Faivovich, J., Garcia, P.C.A., Ananias, F., Lanari, L., Basso, N.G. & Wheeler, W.C. (2004) Molecular perspective on the phylogeny of Hyla pulchella species group (Anura, Hylidae). Molecular Phylogenetics and Evolution, 32 (3), 938–950. https://doi.org/10.1016/j.ympev.2004.03.008

    Faivovich, J., Haddad, C.F.B., Garcia, P.C.A., Frost, D.R., Campbell, J.A. & Wheeler, W.C. (2005) Systematic Review of the Frog Family Hylidae, with special reference to Hylinae: Phylogenetic analysis and Taxonomic Revision. Bulletin of the American Museum of Natural History, 294 (1), 1–240.

    https://doi.org/10.1206/0003-0090(2005)294[0001:SROTFF]2.0.CO;2

    Faivovich, J., McDiarmid, R.W. & Myers, C.W. (2013) Two new species of Myersiohyla (Anura: Hylidae) from Cerro de la Neblina, Venezuela, with comments on other species of the genus. American Museum Novitates, 3792, 1–63.

    https://doi.org/10.1206/3792.1

    Faivovich, J., Moravec, J., Cisneros-Heredia, D.F. & Köhler, J. (2006) A New Species of the Hypsiboas benitezi Group From the Western Amazon Basin (Amphibia: Anura: Hylidae). Herpetologica, 62 (1), 96–108.

    https://doi.org/10.1655/05-12.1

    Field, R., Hawkins, B.A., Cornell, H.V., Currie, D.J., Filho, J.A.F.D., Guégan, J.F., Kaufman, D.M., Kerr, J.T., Mittelbach, G.G., Oberdorff, T., O’Brien, E.M. & Turner, J.R.G. (2009) Spatial species-richness gradients across scales: a meta-analysis. Journal of Biogeography, 36 (1), 132–147.

    https://doi.org/10.1111/j.1365-2699.2008.01963.x

    Fouquet, A., Cortois, E.A., Baudain, D., Lima, J.D., Souza, S.M., Noonan, B.P. & Rodrigues, M.T. (2015) The trans-riverine genetic structure of 28 Amazonian frog species is dependent of life history. Journal of Tropical Ecology, 31, 361–373.

    https://doi.org/10.1017/S0266467415000206

    Fouquet, A., Gilles, A., Vences, M., Marty, C., Blanc, M. & Gemmell, N.J. (2007) Underestimation of species richness in Neotropical frogs revealed by mtDNA analyses. PLoS ONE, 2, e1109.

    https://doi.org/10.1371/journal.pone.0001109

    Fouquet, A., Martinez, Q., Zeidler, L., Courtois, E.A., Gaucher, P., Blanc, M., Lima, J.D., Souza, S.M., Rodrigues, M.T. & Kok, P.J.R. (2016) Cryptic diversity in the Hypsiboas semilineatus species group (Amphibia, Anura) with description of a new species from eastern Guiana Shield. Zootaxa, 4084 (1), 79–104.

    https://doi.org/10.11646/zootaxa.4084.1.3

    Fouquet, A., Noonan, B.P., Blanc, M. & Orrico, V.G.D. (2011) Phylogenetic position of Dendropsophus gaucheri (Lescure and Marty 2000) highlights the need for an in-depth investigation of the phylogenetic relationships of Dendropsophus (Anura: Hylidae). Zootaxa, 3035 (1), 59–67.

    https://doi.org/10.11646/zootaxa.3035.1.3

    Frost, D.R. (2020) Amphibians Species of the World: an Online Reference. Version 6.1. American Museum of Natural History, New York. Eletronic Database accessible. Available from: https://amphhibiansoftheworld.amnh.org/index.php (accessed 7 April 2020)

    https://doi.org/10.5531/db.vz.0001

    Funk, W.C., Caminer, M. & Ron, S. (2012) High levels of cryptic species diversity uncovered in Amazonian frogs. Proceedings of the Royal Society B, 279, 1806–1814.

    https://doi.org/10.1098/rspb.2011.1653

    Godinho, M.B.D.C. & Da Silva, F.R. (2018) The influence of riverine barriers, climate, and topography on the biogeographic regionalization of Amazonian anurans. Scientific Reports, 8 (1), 1–11.

    https://doi.org/10.1038/s41598-018-21879-9

    Gorzula, S. & Señaris, J.C. (1998) Contribution to the herpetofauna of the Venezuelan Guayana I. A data base. Scientia Guaianae, 8, xviii–270.

    Guarnizo, C.E, Paz, A., Muñoz-Ortiz, A., Flechas, S.V., Méndez-Narváez, J. & Crawford, A.J. (2015) DNA Barcoding Survey of Anurans across the Eastern Cordillera of Colombia and the Impact of the Andes on Cryptic Diversity. PLoS ONE, 10 (5), e0127312.

    https://doi.org/10.1371/journal.pone.0127312

    Grant, T. (2019) Outgroup sampling in phylogenetics: Severity of test and successive outgroup expansion. Journal of Zoological Systematics and Evolutionary Research, 57 (4), 748–763.

    https://doi.org/10.1111/jzs.12317

    Heyer, W.R. (1994) Hyla benitezi (Amphibia: Anura: Hylidae): First Record for Brazil and Its Biogeographical Significance. Journal of Herpetology, 28 (4), 497–499.

    https://doi.org/10.2307/1564964

    Hoogmoed, M. (1979) Resurrection of Hyla ornatissima Noble (Amphibia, Hylidae) and remarks on related species of green tree frogs from the Guiana area. Notes on the Herpetofauna of Surinam VI. Zoologische Verhandelingen Leiden, 172, 1–46.

    Jansen, M., Bloch, R., Schulze, A. & Pfenninger, M. (2011) Integrative inventory of Bolivia’s lowland anurans reveals hidden diversity. Zoologica Scripta, 40 (6), 567–583.

    https://doi.org/10.1111/j.1463-6409.2011.00498.x

    Katoh, K., Rozewicki, J. & Yamada, K.D. (2019) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bionformatics, 20 (4), 1160–1166.

    https://doi.org/10.1093/bib/bbx108

    Kearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., Buxton, S., Cooper, A., Markowitz, S., Duran, C., Thierer, T., Ashton, B., Meintjes, P. & Drummond, A. (2012) Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics, 28 (12), 1647–1649.

    https://doi.org/10.1093/bioinformatics/bts199

    Köhler, J., Koscinski, D., Padial, J.M., Chaparro, J.C., Handford, P., Lougheed, S.C. & De la Riva, I. (2010) Systematic of the Andean gladiator frog of the Hypsiboas pulchellus species group. Zoologica Scripta, 39 (6), 572–590.

    https://doi.org/10.1111/j.1463-6409.2010.00448.x

    Kok, P.J.R. (2006) A new species of Hypsiboas (Amphibia: Anura: Hylidae) from Kaieteur National Park, eastern edge of the Pakaraima Mountains, Guyana. Bulletin de l’Institut Royal des Sciences Naturelles de Belgique, Biologie, 76, 191–200.

    Kok, P.J.R. & Kalamandeen, M. (2008) Introduction to the taxonomy of the amphibians of Kaieteur National Park, Guyana. Abc Taxa, 5, 1–278.

    Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. (2018) MEGAX: Molecular Evolutionary Genetics Analysis across Computing Platforms. Molecular Biology and Evolution, 35, 1547–1549.

    https://doi.org/10.1093/molbev/msy096

    La Marca, E. (1992) Catálogo Taxonómico, Biogeográfico y Bibliográfico de las Ranas de Venezuela. Cuadernos Geográficos ULA 9. Universidade de Los Andes, Mérida, Mérida, 197 pp.

    Lanfear, R., Frandsen, P.B., Wright, A.M., Senfeld, T. & Calcott, B. (2017) PartitionFinder 2: new methods for selecting parti- tioned models of evolution for molecular and morphological phylogenetic analyses. Molecular Biology and Evolution, 34, 772–773.

    https://doi.org/10.1093/molbev/msw260

    Lehr, E., Faivovich, J. & Jungfer, K. (2010) A new andean species of the Hypsiboas pulchella group: adults, calls and phylogenetic relationship. Herpetologica, 66 (3), 296–307.

    https://doi.org/10.1655/09-026.1

    Lima, L.R., Dubeux, M.J.M., Nascimento, F.A.C.D., Bruschi, D.P. & Mott, T. (2019) Uncovering Neotropical treefrog diversity: Integrative taxonomy reveal paraphyly in Boana atlantica (Amphibia, Anura, Hylidae). Amphibia Reptilia, 40 (4), 511–521.

    https://doi.org/10.1163/15685381-20191109

    Lutz, B. (1948) Anfíbios anuros da coleção Adolfo Lutz Trabalho do Instituto Oswaldo Cruz. II. Espécies Verdes do Gênero Hyla do Leste Meridional do Brasil. Memórias do Instituto Oswaldo Cruz, 46 (3), 551–577.

    https://doi.org/10.1590/S0074-02761948000300002

    Lutz, B. (1950) Anfíbios anuros da coleção Adolfo Lutz Trabalho do Instituto Oswaldo Cruz.V. Memórias do Instituto Oswaldo Cruz, 48, 599–637.

    https://doi.org/10.1590/S0074-02761950000100022

    Lynch, J.D. (2005) Discovery of the richest frog fauna in the World - an exploration of the forests to the north of Leticia. Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales, 29 (113), 581–588.

    Lynch, J.D. (2007) Diversidad Biológica del sur de la Amazonía colombiana. In: Ruiz S. L., Sánchez E., Tabares E., Prieto, A., Arias, J.C., Gómez, R., Castellanos, D., García, P. & Rodríguez, L. (Eds.), Diversidad biológica y cultural del sur de la Amazonia colombiana—Diagnóstico. Corpoamazonia. Instituto Humboldt, Instituto Sinchi, UAESPNN, Bogotá, pp. 595–600.

    Lyra, M.L., Haddad, C.F.B. & Azeredo-Espin, A.M.L. (2017) Meeting the challenge of the DNA barcoding Neotropical amphibians: polymerase chain reaction optimization and new COI primers. Molecular Ecology Resources, 17, 966–980.

    https://doi.org/10.1111/1755-0998.12648

    MacCulloch, R.D. & Lathrop, A. (2005) Hylid frogs from Mount Ayanganna, Guyana: New species, redescriptions, and distributional records. Phyllomedusa, 4 (1), 17–37.

    https://doi.org/10.11606/issn.2316-9079.v4i1p17-37

    Mayer, M., da Fonte, L.F.M. & Lötters, S. (2019) Mind the gap! A review of Amazonian anurans in GenBank. Salamandra, 55 (2), 89–96.

    http://doi.org/10.5281/zenodo.2840666

    McDiarmid, R.W., Cocroft, R.B. & Paolillo, A. (1988) Preliminary Field Report. Herpetology Collections—Cerro de la Neblina. In: Brewer-Carias, C. (Ed.), Cerro de la Neblina. Resultados de la Expedition 1983–1987. Fundación para el Desarrollo del Comercio Internacional, Caracas, pp. 665–670.

    McDiarmid, R.W. & Donnelly, M.A. (2005) The herpetofauana of Guayana Highlands: Amphibians and reptiles of the Lost World. In: Donnelly, M.A., Crother, B.I., Guyer, G., Wake, M.H. & White, M.E. (Eds.), Ecology and Evoluition in the tropics: a herpetological perspective. University of Chicago press, Chicago, Illinois, pp. 461–560.

    Menin, M., De Carvalho, V.T., Almeida, A.P., Gordo, M., Oliveira, D.P., Luiz, L.F., Campos, J.V. & Hrbek, T. (2017) Amphibians from Santa Isabel do Rio Negro, Brazilian Amazonia. Phyllomedusa, 16 (2), 183–199.

    https://doi.org/10.11606/issn.2316-9079.v16i2p183-199

    Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. Proceedings of the Gateway Computing Environments Workshop, 2010, 1–8.

    https://doi.org/10.1109/GCE.2010.5676129

    Moritz, C., Patton, J.L., Schneider, C.J. & Smith, T.B. (2000) Diversification of rainforest faunas: an integrated molecular approach. Annual Review of Ecology and Systematics, 31 (1), 533–563.

    https://doi.org/10.1146/annurev.ecolsys.31.1.533

    Morrone, J.J. (2014) Biogeographic regionalization of the Neotropical region. Zootaxa, 3782 (1), 1–110.

    Motta, J., Menin, M., Almeida, A.P., Hrbek, T. & Farias, I.P. (2018) When the unknown lives next door: A study of central Amazonian anurofauna. Zootaxa, 4438 (1), 79–104.

    https://doi.org/10.11646/zootaxa.4438.1.3

    Myers, C.W. & Duellman, W.E. (1982) A new species of Hyla from Cerro Colorado, and other tree frog records and geographical notes from western Panama. American Museum Novitates, 2752, 1–32.

    Myers, C.W. & Donelly M.A. (2008) The summit Herpetofauna of Ayantepui, Venezuela: Report from The Robert G. Goelet American Museum—Terramar Expedition. Bulletin of the American museum of Natural History, 308, 1–147. https://doi.org/10.1206/308.1

    Noble, G.K. (1923 a) New batrachians from the tropical research station British Guiana. Zoologica, III (14), 291–293.

    Noble, G.K. (1923 b) Six new batrachians from the Dominican Republic. American Museum Novitates, 61, 1–6.

    Padial, J.M, Chaparro, J.C., Castroviejo–Fisher, S., Guyasamin, J., Lehr, E., Delgado, A., Vaira, M., Teixeira, J.R., Aguayo, R. & De la Riva, I. (2012) A revision of species diversity in the Neotropical genus Oreobates (Anura: Strabomantidae), with the description of three new species from the Amazonian slopes of the Andes. American Museum Novitates, 3755, 1–55.

    https://doi.org/10.1206/3752.2

    Padial, J.M. & De la Riva, I. (2009) Integrative taxonomy reveals cryptic Amazonian species of Pristimantis (Anura: Strabomantidae). Zoological Journal of the Linnean Society, 155, 97–122.

    https://doi.org/10.1111/j.1096-3642.2008.00424.x

    Padial, J.M., Miralles, A., De la Riva, I. & Vences, M. (2010) The integrative future of taxonomy. Frontiers in Zoology, 7, 16.

    https://doi.org/10.1186/1742-9994-7-16

    Padial, J.M., Grant, T. & Frost, D.R. (2014) Molecular systematics of terraranas (Anura: Brachycephaloidea) with an assessment of the effects of alignment and optimal criteria. Zootaxa, 3825 (1), 1–132.

    https://doi.org/10.11646/zootaxa.3825.1.1

    Palumbi, S.R. (1996) Nucleic acids II: the polymerase chain reaction. In: Hillis, D.M., Moritz, C. & Mable, B.K. (Eds.), Molecular Systematics. Sinauer & Associates Inc., Sunderland, Massachusetts, pp. 205–247.

    Peloso, P.L.V., Oliveira, R.M., Sturaro, M.J., Rodrigues, M.T., Lima-Filho, G.R., Bitar, Y.O.C., Wheeler, W.C. & Aleixo, A. (2018) Phylogeny of map tree frogs, Boana semilineata species group, with new Amazonian species (Anura: Hylidae). South American Journal of Herpetology, 13 (2), 150–169.

    http://doi.org/10.2994/SAJH-D-17-00037.1

    Pinheiro, P.D.P., Kok, P.J.R., Noonan, B.P., Means, D.B., Haddad, C.F.B. & Faivovich, J. (2019) A new genus of Cophomantini, with comments on the taxonomic status of Boana liliae (Anura: Hylidae). Zoological Journal of the Linnean Society, 185, 226–245.

    https://doi.org/10.1093/zoolinnean/zly030

    Pyburn, W.F. (1977) A New Hylid Frog (Amphibia, Anura, Hylidae) from the Vaupes River of Colombia with Comments on Related Species. Journal of Herpetology, 11 (4), 405–410.

    https://doi.org/10.2307/1562722

    Pyburn, W.F. (1978) The voice and relationship of the treefrog Hyla hobbsi (Anura: Hylidae). Proceedings of the Biological Society of Washington, 91, 123–131.

    Pyron, R.A. & Wiens, J.J. (2011) A large-scale phylogeny of Amphibia including over 2800 species, and a revised classification of extant frogs, salamanders, and caecilians. Molecular Biology and Evolution, 61, 543–583.

    https://doi.org/10.1016/j.ympev.2011.06.012

    Rambaut, A., Drummond, A.J., Xie, D., Baele, G. & Suchard, M.A. (2018) Posterior summarization in Bayesian phylogenetics using Tracer 1.7. Systematic Biology, 67, 5, 901–904.

    https://doi.org/10.1093/sysbio/syy032

    Ramos, E.K.S., Magalhães, R.F., Marques, N.C.S., Baêta, D., Garcia, P.C.A. & Santos, F.R. (2019) Cryptic diversity in Brazilian endemic monkey frogs (Hylidae, Phyllomedusinae, Pithecopus) revealed by multispecies coalescent and integrative approaches. Molecular Phylogenetics and Evolution, 132, 105–116.

    https://doi.org/10.1016/j.ympev.2018.11.022

    Rivero, J.A. (1961). Salentia of Venezuela. Bulletin of the Museum of Comparative Zoology, 26 (1), 1–207.

    Rivero, J.A. (1964) Salientios (Amphibia) en la Coleccion de la Sociedad de Ciencias Naturales la Salle de Venezuela. Caribbean Journal of Science, 4 (1), 297–305.

    Rivero, J.A. (1972) Notas sobre los anfibios de Venezuela. I. Sobre los hilídos de la Guayana Venezolana. Caribbean Journal of Science, 11, 181–193.

    Rojas, R.R., Fouquet, A., Ron, S.R., Hernandéz-Ruz, E.J., Melo-Sampaio, P.R., Chaparro, J.C., Vogt, R.C., Carvalho, V.T., Pinheiro, L.C., Ávila, R.W., Farias, I.P., Gordo, M. & Hrbek, T. (2018) A Pan-Amazonian species delimitation: high species diversity within the genus Amazophrynella (Anura: Bufonidae). PeerJ, 6, e4941. [https://peerj.com/articles/4941]

    https://doi.org/10.7717/peerj.4941

    Ronquist, F., Teslenko, M., Van Der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61, 539–542.

    https://doi.org/10.1093/sysbio/sys029

    Ruiz-Carranza, P.M., Ardila-Robayo, M.C. & Lynch, J.D. (1996) Lista actualizada de la fauna de Amphibia de Colombia. Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales, 20 (77), 365–415.

    Salducci, M., Marty, C., Chappaz, R. & Gilles, A. (2002) Molecular phylogeny of French Guiana Hylinae: implications for the systematic and biodiversity of the Neotropical frogs. Comptes Rendus—Biologies, 325 (2), 141–153.

    https://doi.org/10.1016/S1631-0691(02)01423-3

    Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Laurel Hollow, New York, 545 pp.

    Savage, J.M. & Heyer, R.W. (1967) Variation and distribution of the tree-frog genus Phyllomedusa in Costa Rica, Central America. Beiträge zur Neotropischen Fauna, 5, 111–131.

    https://doi.org/10.1080/01650526709360400

    Schneider, J.G. (1799) Historia Amphibiorum Naturalis et Literarariae. Fasciculus Primus. Continens Ranas, Calamitas, Bu- fones, Salamandras et Hydros in Genera et Species Descriptos Notisque suis Distinctos. Friederici Frommanni, Jena, 264 pp.

    https://doi.org/10.5962/bhl.title.78757

    Señaris, J.C. & Ayarzagüena, J. (2002) A New Species of Hyla (Anura: Hylidae) from the Highlands of Venezuelan Guayana. Journal of Herpetology, 36 (4), 634–640.

    https://doi.org/10.2307/1565934

    Señaris, J.C. & Ayarzagüena, J. (2006) A new species of Hypsiboas (Amphibia; Anura; Hylidae) from the Venezuelan Guayana, with notes on Hypsiboas sibleszi (Rivero 1972). Herpetologica, 62, 309–319.

    https://doi.org/10.1655/0018-0831(2006)62[308:ANSOHH]2.0.CO;2

    Señaris, J.C., Lampo, M., Rojas-Runjaic, F.J.M. & Barrio-Amorós , C.L. (2014) Guía ilustrada de los anfíbios del Parque Nacional Canaima, Venezuela. Ediciones IVIC, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, 264 pp.

    Simões, P.I., Rojas-Runjaic, F.M., Gagliardi-Urrutia, G. & Castroviejo-Fisher, S. (2019) Five new country records of Amazonian anurans for Brazil, with notes on morphology, advertisement calls, and natural history. Herpetology Notes, 12, 211–219.

    Spix, J.B. (1824) Animalia nova sive species novae Testudinum et Ranarum, quas in itinere per Brasiliam annis MDCCCXVII–MDCCCXX jussu et auspiciis Maximiliani Josephi I. Bavariae Regis. Typis Franc. Seraph. Hübschmanni, Monachii, 53 pp.

    https://doi.org/10.5962/bhl.title.63182

    Suárez-Mayorga, A.M. & Lynch, J.D. (2017) Myth and truth on the herpetofauna of Chiribiquete: from the lost world to the last world. Revista Colombia Amazónica, 10, 177–190.

    Sturaro, M.J., Costa, J.C.L., Maciel, A.O., Lima-Filho, G.R., Rojas-Runjaic, F.J.M., Mejia, D.P. & Peloso, P.L.V. (2020) Resolving the taxonomic puzzle of Boana cinerascens (Spix, 1824), with resurrection of Hyla granosa gracilis Melin, 1941 (Anura:Hylidae). Zootaxa, 4750 (1), 1, 1–30.

    https://doi.org/10.11646/zootaxa.4750.1.1

    Taboada, C., Brunetti, A.E., Lyra, M.L., Fitak, R.R., Soverna, A.F., Ron, S.R., Lagorio, M.G., Haddad, C.F.B., Lopes, N.P., Johsen, S., Faivovich, J., Chemes, L.B. & Bari, S.E. (2020) Multiple origins of green coloration in frogs mediated by a novel biliverdin-biding serpin. Proceedings of the National Academy of Sciences, 117, 31, 18574–18581.

    https://doi.org/10.1073/pnas.2006771117

    Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673–4680.

    https://doi.org/10.1093/nar/22.22.4673

    Vences, M., Meike, T., Van der Meijden, A., Chiari, Y. & Vieites, D. (2005) Comparative performance of the 16S rRNA gene in DNA barcoding of amphibians. Frontiers in Zoology, 2, 7–12.

    https://doi.org/10.1186/1742-9994-2-5

    von May, R., Catenazzi, A., Santa-Cruz, R., Gutierrez, A.S., Moritz, C. & Rabosky, D.L. (2019) Thermal physiological traits in tropical lowland amphibians: Vulnerability to climate warming and cooling. PLoS ONE, 14 (8), 1–18.

    https://doi.org/10.1371/journal.pone.0219759

    Watters, J.L., Cummings, S.T., Flanagan, R.L. & Siler, C.D. (2016) Review of morphometric measurements used in anuran species descriptions and recommendations for a standardized approach. Zootaxa, 4072 (4), 477–495.

    https://doi.org/10.11646/zootaxa.4072.4.6

    Whittaker, R.J., Araújo, M.B., Paul, J., Ladle, R.J., Watson, J.E.M. & Willis, K.J. (2005) Conservation biogeography: Assessment and prospect. Diversity and Distribution, 11, 3–23.

    https://doi.org/10.1111/j.1366-9516.2005.00143.x

    Wiens, J.J., Fetzner Jr., J.W., Parkinson, C.L. & Reeder, T. (2005) Hylid frogs phylogeny and sampling strategies for speciose clades. Systematic Biology, 54 (5), 719–748.

    https://doi.org/10.1080/10635150500234625

    Wiens, J.J., Kuczynski, C.A., Hua, X. &Moen, D.S. (2010) An expanded phylogeny of treefrogs (Hylidae) based on nuclear and mitochondrial sequence data. Molecular Phylogenetics and Evolution, 55, 871–882.

    https://doi.org/10.1016/j.ympev.2010.03.013