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Issue: Vol. 5005 No. 1: 23 Jul. 2021
Type: Article
Published: 2021-07-23
DOI: 10.11646/zootaxa.5005.1.3
Page range: 41-61
Abstract views: 567
PDF downloaded: 18

Three uncharted endemic earthworm species of the genus Eutyphoeus (Oligochaeta: Octochaetidae) from Mizoram, India

Department of Zoology, Dr. Harisingh Gour Vishwavidyalaya (A Central University), Sagar—470003, Madhya Pradesh, India
Department of Zoology, Dr. Harisingh Gour Vishwavidyalaya (A Central University), Sagar—470003, Madhya Pradesh, India
Department of Biotechnology, Dr. Harisingh Gour Vishwavidyalaya (A Central University), Sagar—470003, Madhya Pradesh, India
Sustainable Living, Maharishi International University, Fairfield Iowa, United States
Department of Zoology, Dr. Harisingh Gour Vishwavidyalaya (A Central University), Sagar—470003, Madhya Pradesh, India
coi marker DNA barcoding new species biodiversity taxonomy north-eastern region India Annelida

Abstract

Integrative approach of taxonomy drives us to the discovery of three new species of the genus Eutyphoeus Michaelsen 1900, namely Eutyphoeus phawngpuiensis sp. nov., Eutyphoeus serei sp. nov. and Eutyphoeus tawi sp. nov. from protected forests of Mizoram, India. E. phawngpuiensis sp. nov. belongs to the Eutyphoeus hastatus group, and is characterized by holandric male organs, having spermathecal pores at “a or ab”, bidiverticulate spermathecae, and lack of genital marking glands. While, E. tawi sp. nov. has spermathecal pores at b or lateral to b, without lateral intestinal caeca. E. serei sp. nov. belongs to the Eutyphoeus gigas group, and is distinguished by having metandric male organs, lacking female pore of right side, and having spoon shaped tip of penial setae without ornamentation. Molecular characterization using coi gene marker also supports the findings. The maximum intraspecific distances and distance to the nearest neighbour of E. phawngpuiensis sp. nov., E. serei sp. nov. and E. tawi sp. nov. were recorded 3.5%, 9.37%; 2.35%, 14.83%; and 2.28%, 12.86% respectively. The key to Eutyphoeus species of India was also updated.

References

  1. Beddard, F.E. (1883) Note on some earthworms from India. Annals and Magazine of Natural History, Series 6, 12 (70), 213– 224. https://doi.org/10.1080/00222938309459619

    DOI: https://doi.org/10.1080/00222938309459619

  2. Beddard, F.E. (1888) On the structure of three new species of earthworms, with remarks on certain points of the morphology of the Oligochaeta. Quarterly Journal of Microscopical Sciences, 29, 101–131. https://doi.org/10.1242/jcs.s2-29.114.101

    DOI: https://doi.org/10.1242/jcs.s2-29.114.101

  3. Beddard, F.E. (1900) On a new species of earthworm from India, belonging to the Genus Amyntas. Proceedings of the Zoological Society of London, 1990, 998–1002.      

  4. Čandek, K. & Kuntner, M. (2015) DNA barcoding gap: reliable species identification over morphological and geographical scales. Molecular Ecology Resources, 15 (2), 268–277. https://doi.org/10.1111/1755-0998.12304

    DOI: https://doi.org/10.1111/1755-0998.12304

  5. Edgar, R.C. (2004) MUSCLE: a multiple sequence alignment method with reduced time and space complexity. BMC Bioinformatics, 5 (1), 113. https://doi.org/10.1186/1471-2105-5-113

    DOI: https://doi.org/10.1186/1471-2105-5-113

  6. Gates, G.E. (1933) The earthworms of Burma IV. Records of the Indian Museum, 35, 413–606.

  7. Gates, G.E. (1958) The earthworms of Burma. VII. The genus Eutyphoeus, with notes on several Indian species. Records of the Indian Museum, 53, 93–222.

  8. Gates, G.E. (1959) On a taxonomic puzzle and the classification of the earthworms. Bulletin of the Museum of Comparative Zoology, 121, 229–261.

  9. Gates, G.E. (1972) Burmese earthworms. An introduction to the systematics and biology of megadrile oligochaetes with special reference to southeast Asia. Transactions of the American Philosophical Society, 62 (7), 1–326.  https://doi.org/10.2307/1006214

    DOI: https://doi.org/10.2307/1006214

  10. Halder, K.R., Dhani, S. & Mandal, C.K. (2007) On some earthworms present in unnamed collections of Zoological Survey of India. Records of the Zoological Survey of India, 107, 79–93.

  11. Hebert, P.D., Cywinska, A., Ball, S.L. & Dewaard, J.R. (2003) Biological identifications through DNA barcodes. Proceedings of the Royal Society of London, Series B: Biological Sciences, 270 (1512), 313–321. https://doi.org/10.1098/rspb.2002.2218

    DOI: https://doi.org/10.1098/rspb.2002.2218

  12. James, S.W., Porco, D., Decaëns, T., Richard, B., Rougerie, R. & Erséus, C. (2010) DNA barcoding reveals cryptic diversity in Lumbricus terrestris L., 1758 (Clitellata): resurrection of L. herculeus (Savigny, 1826). Plos One, 5 (12), e15629. https://doi.org/10.1371/journal.pone.0015629

    DOI: https://doi.org/10.1371/journal.pone.0015629

  13. Jeratthitikul, E., Bantaowong, U. & Panha, S. (2017) DNA barcoding of the Thai species of terrestrial earthworms in the genera Amynthas and Metaphire (Haplotaxida: Megascolecidae). European Journal of Soil Biology, 81, 39–47. https://doi.org/10.1016/j.ejsobi.2017.06.004

    DOI: https://doi.org/10.1016/j.ejsobi.2017.06.004

  14. Julka, J.M. (1988) The Fauna of India and the adjacent countries. Megadrile Oligochaeta (Earthworms). Haplotaxida: Lumbricina: Megascolecoidea: Octochaetidae. Records of the Zoological Survey of India, Calcutta, 400 pp.

  15. Julka, J.M., Ramanujam, S.N. & Lalthanzara, H. (2005) On a new species of earthworm genus Eutyphoeus (Octochaetidae: Oligochaeta) from Mizoram, India. Megadrilogica, 10, 69–74.

  16. Kimura, M. (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16 (2), 111–120. https://doi.org/10.1007/BF01731581

    DOI: https://doi.org/10.1007/BF01731581

  17. Kinberg, J.G.H. (1867) Annulata nova. Öfversigt af Königlich Vetenskaps-Akademiens Förhandlingar, Stockholm, 23 (9), 97–103 + 337–357.

  18. Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35 (6), 1547–1549. https://doi.org/10.1093/molbev/msy096

    DOI: https://doi.org/10.1093/molbev/msy096

  19. Kvist, S. (2016) Does a global DNA barcoding gap exist in Annelida? Mitochondrial DNA Part A, 27 (3), 2241–2252. https://doi.org/10.3109/19401736.2014.984166

  20. Lalthanzara, H., Ramanujam, S.N. & Jha, L.K. (2011) Population dynamics of earthworms in relation to soil physico-chemical parameters in agroforestry systems of Mizoram, India. Journal of Environmental Biology, 32, 599–605.

  21. Lalthanzara, H. & Ramanujam, S.N. (2014) Earthworm cast production and physico-chemical properties in two agroforestry systems of Mizoram (India). Tropical Ecology, 55, 77–86.

  22. Lewis, T. & Taylor, L.R. (1968) Introduction to experimental ecology. Academic Press. London, New York, 401 pp.

  23. Lone, A.R., Tiwari, N., Thakur, S.S., Pearlson, O., Pavlíček, T. & Yadav, S. (2020) Exploration of four new Kanchuria sp. of earthworms (Oligochaeta: Megascolecidae) from the North Eastern Region of India using DNA bar-coding approach. Journal of Asia-Pacific Biodiversity, 13 (2), 268–281. https://doi.org/10.1016/j.japb.2020.02.004

    DOI: https://doi.org/10.1016/j.japb.2020.02.004

  24. Michaelsen, W. (1900) Oligochaeta. In: Das Tierreich. Vol. 10. R. Friedlander und Sohn, Berlin, pp. 1–575.

  25. Michaelsen, W. (1907) Neue Oligochaeten von Vorder-Indien, Ceylon, Birma, und den Andaman-Inseln. Jahrbuch der Hamburgischen Wissenschaftlichen Anstalten, Hamburg, 24 (2), 143–188.

  26. Perrier, E. (1872) Recherche pour servir à l‘historie des Lombriciens terrestres. Nouvelles Archives du Museum d’Histoire Naturelle Paris, 8, 5–198. https://doi.org/10.5962/bhl.title.12201

    DOI: https://doi.org/10.5962/bhl.title.12201

  27. Porco, D., Chang, C.H., Dupont, L., James, S., Richard, B. & Decaëns, T. (2018) A reference library of DNA barcodes for the earthworms from Upper Normandy: Biodiversity assessment, new records, potential cases of cryptic diversity and ongoing speciation. Applied Soil Ecology, 124, 362–371. https://doi.org/10.1016/j.apsoil.2017.11.001

    DOI: https://doi.org/10.1016/j.apsoil.2017.11.001

  28. Puillandre, N., Lambert, A., Brouillet, S. & Achaz, G. (2012) ABGD, Automatic Barcode Gap Discovery for primary species delimitation. Molecular Ecology, 21 (8), 1864–1877.  https://doi.org/10.1111/j.1365-294X.2011.05239.x

    DOI: https://doi.org/10.1111/j.1365-294X.2011.05239.x

  29. Ramanujam, S.N., Lalthanzara, H. & Jha, L.K. (2004) Biodiversity of earthworms in Mizoram. Journal of Nature Conservators, 16, 129–134.

  30. Ratnasingham, S. & Hebert, P.D. (2007) BOLD: The Barcode of Life Data System (http://www. barcodinglife. org). Molecular Ecology Notes, 7 (3), 355–364. https://doi.org/10.1111/j.1471-8286.2007.01678.x

    DOI: https://doi.org/10.1111/j.1471-8286.2007.01678.x

  31. Ratnasingham, S. & Hebert, P.D. (2013) A DNA-based registry for all animal species: the Barcode Index Number (BIN) system. Plos One, 8 (7), e66213. https://doi.org/10.1371/journal.pone.0066213

    DOI: https://doi.org/10.1371/journal.pone.0066213

  32. Stephenson, J. (1917) On a collection of Oligochaeta from various parts of India and further India. Records of the Indian Museum, 13, 353–416.  https://doi.org/10.5962/bhl.part.5865

    DOI: https://doi.org/10.5962/bhl.part.5865

  33. Stephenson, J. (1921) Oligochaeta from Manipur, the Laccadive Islands, Mysore, and other parts of India. Records of the Indian Museum, 22, 745–768. https://doi.org/10.5962/bhl.part.1484

    DOI: https://doi.org/10.5962/bhl.part.1484

  34. Stephenson, J. (1923) Oligochaeta. The Fauna of British India, including Ceylon and Burma. Taylor and Francis, London, 518 pp.

  35. Stephenson, J. (1926) Descriptions of Indian Oligochaeta. I. Records of the Indian Museum, 28, 249–267.

  36. Tamura, K. & Nei, M. (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution, 10 (3), 512–526.

  37. Thakur, S.S. & Yadav, S. (2018) Exploration of Earthworms of India through Online Digital Library. Earthworms: The Ecological Engineers of Soil, 2018, 33–59. [published online] https://doi.org/10.5772/intechopen.75666

    DOI: https://doi.org/10.5772/intechopen.75666

  38. Thakur, S.S., Lone, A.R., Tiwari, N. & Yadav, S. (2020) Exploring new records of Eutyphoeus sp. (Haplotaxida: Octochaetidae) from garo hills, Meghalaya, North Eastern state of India with use of DNA barcodes. Mitochondrial DNA Part A, 31 (7), 265–272.  https://doi.org/10.1080/24701394.2020.1781834

    DOI: https://doi.org/10.1080/24701394.2020.1781834

  39. Tiwari, N., Paliwal, R., Rashid, A. & Yadav, S. (2020) Checklist of earthworm species (Oligochaeta) of the North Eastern Region of India. Zootaxa, 4772 (2), 277–305. https://doi.org/10.11646/zootaxa.4772.2.3

    DOI: https://doi.org/10.11646/zootaxa.4772.2.3

  40. Tiwari, N., Lone, A.R., Thakur, S.S. & Yadav, S. (2021) Interrogation of earthworm (Clitellata: Haplotaxida) taxonomy and the DNA sequence database. Journal of Asia-Pacific Biodiversity, 14 (1), 40–52.  https://doi.org/10.1016/j.japb.2020.09.015

    DOI: https://doi.org/10.1016/j.japb.2020.09.015