Abstract
Eptesicus diminutus Osgood, 1915 is the smallest species of the subgenus Eptesicus found in South America. It has a discontinuous distribution, with northern populations in Colombia and Venezuela, and southern populations in Argentina, Brazil, Paraguay, and Uruguay. We reviewed specimens assigned to the northern populations of E. diminutus and compared them to these from the southern populations. Based on morphologic, morphometric, and molecular analyses, we support the recognition of the northern populations of E. diminutus as a new species. This new species is distributed in the Llanos of the Orinoco region in Colombia and Venezuela (and likely in Guyana). Eptesicus sp. nov. is morphologically similar to E. diminutus and E. furinalis, from which it can be distinguished by its intermediate size. This taxon increases to 11 the number of species of bats of the subgenus Eptesicus in South America.
References
Acosta S., L.H., Poma Urey, J.L., Ossa-López, P.A., Rivera-Páez, F. & Ramírez-Chaves. H.E. (2021) A new species of Eptesicus Rafinesque, 1820 (Mammalia: Chiroptera: Vespertilionidae), from the sub-Andean Forest of Santa Cruz, Bolivia. Therya, 12, 391–409. https://doi.org/10.12933/therya-21-1119
Alberico, M., Cadena, A., Hernández-Camacho, J. & Muñoz-Saba, Y. (2000) Mamíferos (Synapsida: Theria) de Colombia. Biota Colombiana, 1, 43–75.
Allen, J.A. (1897) Description of a new vespertilionine bat from Yucatan. Bulletin of the American Museum of Natural History, 9, 231–232.
Allen, J.A. (1914) New South American bats and a new Octodont. Bulletin of the American Museum of Natural History, 33, 381–389.
Allen, J.A. (1915) New South American mammals. Bulletin of the American Museum of Natural History, 34, 625–634.
Baker, R.J. & Bradley, R.D. (2006) Speciation in Mammals and the Genetic Species Concept. Journal of Mammalogy, 87, 643–662. https://doi.org/10.1644/06-MAMM-F-038R2.1
Barquez, R.M., Mares, M.A. & Braun, J.K. (1999) The Bats of Argentina. Special Publications 42. Museum of Texas Tech University, Lubbock, Texas, 275 pp. https://doi.org/10.5962/bhl.title.142628
Bickham, J.W., Patton, J.C., Schlitter, D.A., Rautenbach, I.L. & Honeycutt, R.L. (2004) Molecular phylogenetics, karyotypic diversity, and partition of the genus Myotis (Chiroptera: Vespertilionidae). Molecular Phylogenetics and Evolution, 33, 333–338. https://doi.org/10.1016/j.ympev.2004.06.012
Bickham, J.W., Wood, C.C. & Patton, J.C. (1995) Biogeographic implications of Cytochrome b sequences and allozymes in Sockeye (Oncorhynchus nerka). Journal of Heredity, 86, 140–144. https://doi.org/10.1093/oxfordjournals.jhered.a111544
Burgin, C.J., Colella, J.P., Kahn, P.L. & Upham, N.S. (2018) How many species of mammals are there? Journal of Mammalogy, 99, 1–11. https://doi.org/10.1093/jmammal/gyx147
Caraballo, D.A., Montani, M.E., Martínez, L.M., Antoniazzi, L.R., Sambrana, T.C., Fernández, C., Cisterna, D.M., Beltrán, F.J. & Colombo, V.C. (2020) Heterogeneous taxonomic resolution of cytochrome b gene identification of bats from Argentina: Implications for field studies. PLoS ONE, 15 (12), e0244750.
Clare, E.L., Lim, B.K., Fenton, M.B. & Hebert, P.D.N. (2011) Neotropical Bats: estimating species diversity with DNA Barcodes. PLoS ONE, 6, e22648. https://doi.org/10.1371/journal.pone.0244750
Clement, M., Snell, Q., Walke, P., Posada, D. & Crandall, K. (2002) TCS: estimating gene genealogies. Proceedings 16th International Parallel and Distributed Processing Symposium, 2002, 1–7. https://doi.org/10.1109/IPDPS.2002.1016585
Davis, W.B. (1965) Review of the Eptesicus brasiliensis complex in Middle America with the description of a new subspecies from Costa Rica. Journal of Mammalogy, 46, 229–240. https://doi.org/10.2307/1377842
Davis, W.B. (1966) Review of South American bats of the genus Eptesicus. The Southwestern Naturalist, 11, 245–274. https://doi.org/10.2307/3669648
Davis, W.B. & Gardner, A.L. (2008 [2007]) Genus Eptesicus Rafinesque, 1820. In: Gardner, A.L. (Ed.), Mammals of South America Volume 1. Marsupials, Xenarthrans, Shrews, and Bats. The University of Chicago Press, Chicago, pp. 440–450. [published 31 March 2008] https://doi.org/10.7208/chicago/9780226282428.001.0001
Desmarest, A.G. (1819) Vespertilion. In: Nouveau dictionnaire d’histoire naturelle, appliquée aux arts, à l’agriculture, à l’économie rurale et domestique, à lamédecine, etc. Par une société de naturalistes et d’agriculteurs. Vol. 35. Nouv. éd. Deterville, Paris, pp. 1–572.
d’Orbigny, A. & Gervais, P. (1847) Mammifères. In: d’Orbigny, A. (Ed.), Voyage dans l’Amérique méridionale (le Brésil, la République orientale de l’Uruguay, la République Argentine, la Patagonie, la République du Chili, la République de Bolivia, la République du Pérou), exécuté pendant les annees 1826, 1827, 1828, 1829, 1830, 1831, 1832 et 1833. Vol. 4. P. Bertrand, Paris and V. Levrault, Strasbourg, pp. 1–32. https://doi.org/10.5962/bhl.title.100771
Genoways, H.H. & Baker, R.J. (1996) A new species of the genus Rhogeessa, with comments on geographic distribution and speciation in the genus. In: Genoways, H.H. & Baker, R.J. (Eds.), Contributions in Mammalogy: A memorial volume honoring Dr. J. Knox Jones, Jr. Museum of Texas Tech University, Lubbock, Texas, pp. 83–87.
Gervais, P. (1841) Mammiféres. In Zoologie, ed. Eydoux, J. F. T. & L. F. A. Souleyet, 1–68. In: Voyage autor du Monde exécuté pendant les années 1836 et 1837 sur la corvette La Bonite commandée par M. Vaillant. Arthus Bertrand, Paris, pp. 1–334.
Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/97/NT. Nucleic Acids Symposium Series, 41, 95–98.
Hammer, Ø., Harper, D.A.T. & Ryan, P.D. (2001) PAST: paleontological statistics software package for education and data analysis. Palaeontologia Electronica, 4, 1–9.
Hamilton, P.B., Webster, T.M.U., Basiewicz, M., Kennedy, E.V., De-Bastos, E.S.R. & Mathews, F. (2015) A rapid PCR-based test for identification of fifteen British bat species. Conservation Genetics Resources, 7, 651–657. https://doi.org/10.1007/s12686-015-0470-2
Handley, C.O. Jr. (1976) Mammals of the Smithsonian Venezuelan Project. Brigham Young University Science Bulletin, Biological Series, 20, 1–89. https://doi.org/10.5962/bhl.part.5667
Handley, C.O. Jr. & Gardner, A.L. (2008 [2007]) Genus Histiotus P. Gervais, 1856 In: Gardner, A.L. (Ed.), Mammals of South America. Vol. 1. Marsupials, Xenarthrans, Shrews, and Bats. The University of Chicago Press, Chicago, Illinois, pp. 450–457. [Published 31 March 2008]
Ibáñez, C.J. (1984 [1981]) Biología y ecología de los murciélagos del Hato “El Frio” Apure, Venezuela. Doñana Acta Vertebrata, 8 (4), 1–271.
Juste, J., Benda, P., García-Mudarra, J.L. & Ibáñez, C. (2012) Phylogeny and systematics of Old World serotine bats (genus Eptesicus, Vespertilionidae, Chiroptera): An integrative approach. Zoologica Scripta, 42, 441–457. https://doi.org/10.1111/zsc.12020
Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. (2018) MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution, 35, 1547–1549. https://doi.org/10.1093/molbev/msy096
Lanfear, R., Frandsen, P. B., Wright, A.M., Senfeld, T. & Calcott, B. (2016) PartitionFinder 2: new methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Molecular Biology and Evolution, 34, 772–773. https://doi.org/10.1093/molbev/msw260
Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. Proceedings of the Gateway Computing Environments Workshop (GCE), 2010, 1–8. https://doi.org/10.1109/GCE.2010.5676129
Miranda J.M., Bernardi, I.P. & Passos, F.C. (2006) A new species of Eptesicus (Mammalia: Chiroptera: Vespertilionidae) from the Atlantic Forest, Brazil. Zootaxa, 1383 (1), 57–68. https://doi.org/10.11646/zootaxa.1383.1.4
Morales-Martínez, D.M., Rodríguez-Posada, M.E., Fernández-Rodríguez, C., Calderón-Capote, M.C. & Gutiérrez-Sanabria, D.R. (2018) Spatial variation of bat diversity between three floodplain savanna ecosystems of the Colombian Llanos. Therya, 9, 41–52. https://doi.org/10.12933/therya-18-537
Moratelli, R., Peracchi, A.L., Dias, D. & Oliveira, J.A. (2011) Geographic variation in South American populations of Myotis nigricans (Schinz, 1821) (Chiroptera, Vespertilionidae), with the description of two new species. Mammalian Biology, 76, 592–607. https://doi.org/10.1016/j.mambio.2011.01.003
Moratelli, R., Gardner, A.L., Oliveira, J.A. & Wilson, D.E. (2013) Review of Myotis (Chiroptera, Vespertilionidae) from northern South America, with the description of a new species. American Museum Novitates, 3780, 1–36. https://doi.org/10.1206/3780.2
Moratelli, R., Burgin, C., Cláudio, V., Novaes, R., López-Baucells, A. & Haslauer, R. (2019) Family Vespertilionidae. In: Wilson, D.E. & Mittermeier, R.A. (Eds.), Handbook of the Mammals of the World. Vol. 9. Bats. Lynx Edicions, Barcelona, pp. 855–996.
Muñoz, J. (2001) Murciélagos de Colombia, sistemática, distribución, descripción, historia natural y ecología. Editorial Universidad de Antioquia, Medellín, 391 pp.
Muñoz-Saba, Y., López-Arévalo, H.F. &. Cadena-G., A. (2000) Aportes al conocimiento de la ecología de los murciélagos de los afloramientos de mármoles y calizas, sector de Río Claro (Antioquia, Colombia). Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales, 23 (Suplemento Especial), 651–658. [dated 1999, Published 2000]
Ochoa, G.J., Castellanos, H. & Ibáñez, C. (1988) Records of bats and rodents from Venezuela. Mammalia, 52, 175–180. https://doi.org/10.1515/mamm.1988.52.2.175
Osgood, W.H. (1915) New mammals from Brazil and Peru. Field Museum of Natural History Publications, Zoological Series, 10, 187–198. https://doi.org/10.5962/bhl.title.2556
Paglia, A.P., Fonseca, G.A.B. da, Rylands, A.B., Herrmann, G., Aguiar, L.M.S., Chiarello, A.G., Leite, Y.L.R., Costa, L.P., Siciliano, S., Kierulff, M.C.M., Mendes, S.L., Tavares, V. da C., Mittermeier, R.A. & Patton J.L. (2012) Lista anotada dos Mamíferos do Brasil/Annotated Checklist of Brazilian Mammals. 2ª Edição/2nd Edition. Occasional Papers in Conservation Biology, No. 6. Conservation International, Arlington, Virginia, 76 pp.
Palisot de Beauvois, A.M.F.J. (1796) Catalogue raisonné du museum, de Mr. C. W. Peale, membre de la société philosophique de Pensylvanie. De l’Imprimerie de Parent, Philadelphie, Pennsylvania, 142 pp.
Patterson, B.D., Solari S. & Velazco, P.M. (2012) The role of the Andes in the diversification and biogeography of Neotropical mammals In: Patterson, B.D. & Costa, L.P. (Eds.), Bones, clones, and biomes: the history and geography of recent Neotropical mammals. The University of Chicago Press, Chicago, Illinois, pp. 351–378. https://doi.org/10.7208/chicago/9780226649214.003.0015
Paynter, R.A. (1982) Ornithological Gazetteer of Venezuela. Museum of Comparative Zoology, Harvard University, Cambridge, 245 pp. https://doi.org/10.5962/bhl.title.14633
Paynter, R.A. (1997) Ornithological gazetteer of Colombia. 2nd Edition. Museum of Comparative Zoology, Cambridge, 537 pp. https://doi.org/10.5962/bhl.title.14638
Polanco-Ochoa, R., Jaimes, V. & Piragua, W. (2000) Los mamíferos del Parque Nacional Natural La Paya, Amazonía Colombiana. Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales, 23 (Suplemento Especial), 671–682. [dated 1999, Published 2000]
R Development Core Team. (2009) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna. Available from: http://www.r-project.org/ (accessed 26 July 2021)
Ramírez-Chaves, H.E. (2008) Revisión taxonómica de los murciélagos del género Eptesicus Rafinesque, 1820 (Mammalia: Chiroptera: Vespertilionidae) para Colombia. Tesis de Maestría, Universidad Nacional de Colombia, Bogotá, 81 pp.
Ramírez-Chaves, H.E., Noguera-Urbano, E.A. & Rodríguez-Posada, M.E. (2013) Mamíferos (Mammalia) del departamento de Putumayo, Colombia. Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales, 37, 263–286. https://doi.org/10.18257/raccefyn.9
Rodríguez-Mahecha, J., Hernández-Camacho, J., Defler, R., Alberico, M., Mast, R., Mittermeier, R. & Cadena, A. (1995) Mamíferos Colombianos: Sus nombres comunes e indígenas. Occasional Papers in Conservation Biology No. 3. Conservation International, Santafé de Bogotá, 56 pp.
Rodríguez-Posada, M.E., Morales-Martínez, D.M., Ramírez-Chaves, H.E., Martínez-Medina, D. & Calderón Acevedo, C.A. (2021) A new species of Long-eared Brown Bat of the genus Histiotus (Chiroptera) and the revalidation of Histiotus colombiae. Caldasia, 43. [published online] https://doi.org/10.15446/caldasia.v43n2.85424
Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574. https://doi.org/10.1093/bioinformatics/btg180
Ruedi, M. & Mayer, F. (2001) Molecular Systematics of bats of the genus Myotis (Vespertilionidae) suggests deterministic ecomorphological convergences. Molecular Phylogenetics and Evolution, 21, 436–448. https://doi.org/10.1006/mpev.2001.1017
Sánchez, H.J. & Lew, D. (2012) Lista actualizada y comentada de los mamíferos de Venezuela. Memoria de la Fundación La Salle de Ciencias Naturales, 173–174, 173–238.
Sánchez, R.T., Montani, M.E., Tomasco, I.H., Díaz, M.M. & Barquez, R.M. (2019) A new species of Eptesicus (Chiroptera, Vespertilionidae) from Argentina. Journal of Mammalogy, 100, 118–129. https://doi.org/10.1093/jmammal/gyz009
Simmons, N.B. & Cirranello, A.L. (2020) Bat species of the world: a taxonomic and geographic database. Available from: https://batnames.org/home.html (accessed 27 July 2021)
Simmons, N.B. & Voss, R.S. (1998) The mammals of Paracou, French Guiana: A Neotropical lowland rainforest fauna. Part 1. Bats. Bulletin of the American Museum of Natural History, 237, 1–219.
Thomas, O. (1920) On Neotropical bats of the genus Eptesicus. Annals and Magazine of Natural History, series 9, 5, 360–367. https://doi.org/10.1080/00222932008632387
Turmelle, A.S., Kunz, T.H. & Sorenson, M.D. (2011) A tale of two genomes: contrasting patterns of phylogeographic structure in a widely distributed bat. Molecular Ecology, 20, 357–375. https://doi.org/10.1111/j.1365-294X.2010.04947.x
Williams, C.F. (1978) Taxonomic and kariologic comments on small brown bats, genus Eptesicus, from South America. Annals of Carnegie Museum, 47, 361–368.