Abstract
Luciogobius punctilineatus n. sp. is described on the basis of 21 type specimens from Kyushu, and the Koshiki and Osumi Islands, southern Japan. It is also found in Kochi Prefecture (Shikoku) and Amami-oshima island (Ryukyu Islands), Japan, confirmed by examination of non-type specimens. The genus Luciogobius includes 15 valid and several undescribed species, and most of them inhabit interstitial spaces of stones and gravel in the intertidal zone. The new species is characterized by the following combination of characters: total second dorsal-fin rays 10–12 (modally 11); total anal-fin rays 12–14 (13); pectoral-fin rays 8–12 (10); vertebrae 16–18 + 22–24 = 39–42 (17 + 23 = 40); pectoral-fin posterior margin slightly concave; pelvic fins united, forming a ventral disc; snout relatively short, length 3.1–4.3% of SL; anus to anal-fin origin (AAA) distance twice body depth at anus, 11.4–16.9% of SL; snout length less than 34.7% of AAA distance; pre-anus length less than 85.5% of pre-anal-fin length; single poorly defined black longitudinal line along mid-lateral body region from behind pectoral fin to caudal-fin base, indistinct anteriorly (line embedded, visible through semi-transparent muscle tissue in fresh or live specimens); black spots forming a single longitudinal row on mid-lateral body surface from behind pectoral fin to caudal-fin base (more distinct in preserved specimens).
References
Akihito, Sakamoto, K., Ikeda, Y. & Aizawa, M. (2013) Suborder Gobioidei. In: Nakabo, T. (Ed.), Fishes of Japan with Pictorial Keys to the Species. Third Ed. Tokai University Press, Tokyo, Hadano, pp. 1347–1608 + 2109–2211. [in Japanese]
Gill, T. (1859) Notes on a collection of Japanese fishes, made by Dr. J. Morrow. Proceedings of the Academy of Natural Sciences of Philadelphia, 11, 144–149.
Felsenstein, J. (1981) Evolutionary tree from DNA sequences: a maximum likelihood approach. Journal of Molecular Evolution, 17 (6), 368–376. https://doi.org/10.1007/BF01734359
Ito, T. & Okumura, D. (2021) First records of Luciogobius parvulus and Luciogobius sp. 11 sensu Shibukawa et al., 2019 (Teleostei: Gobiidae) from Tokushima Prefecture, Japan. Ichthy, Natural History of Fishes of Japan, 12, 1–7. [in Japanese] https://doi.org/10.34583/ichthy.12.0_1
Kanagawa, N., Itai, T. & Senou, H. (2011) Two new species of freshwater gobies of the genus Luciogobius (Perciformes: Gobiidae) from Japan. Bulletin of the Kanagawa Prefectural Museum (Natural Science), 40, 67–74.
Kondo, K. & Kato, M. (2018) Life in coastal pebble sediment: unique interstitial organism community and selective feeding on meiobenthos by interstitial fishes (Luciogobius: Gobiidae). bioRxiv. [published online] https://doi.org/10.1101/499194
Koreeda, R. & Motomura, H. (2021) Records of seven temperate and subtropical species of Luciogobius from Shimokoshiki-shima island, Koshiki Islands, East China Sea, Japan, and the first record of the possible hybrid individuals for Luciogobius. Ichthy, Natural History of Fishes of Japan, 11, 27–52. [in Japanese] https://doi.org/10.34583/ichthy.11.0_27
Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. (2018) MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35 (6), 1547–1549. https://doi.org/10.1093/molbev/msy096
Maeda, K. (2014) Luciogobius sp. 2. In: Okiyama, M. (Ed.), An Atlas of Early Stage Fishes in Japan. Second Edition. Tokai University Press, Hadano, pp. 1250–1251. [in Japanese]
Maeda, K., Yamasaki, N., Kondo, M. & Tachihara, K. (2008) Occurrence and morphology of larvae and juveniles of six Luciogobius species from Aritsu Beach, Okinawa Island. Ichthyological Research, 55 (2), 162–174. https://doi.org/10.1007/s10228-007-0018-x
Miya, M., Sato, Y., Fukunaga, T., Sado, T., Poulsen, J.Y., Sato K., Minamoto, T., Yamamoto, S., Yamanaka, H., Araki, H., Kondoh, M. & Iwasaki, W. (2015) MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species. Royal Society Open Science, 2 (7), 155008. https://doi.org/10.1098/rsos.150088
Motomura, H. & Ishikawa, S. (Eds.) (2013) Fish Collection Building and Procedures Manual. English Edition. The Kagoshima University Museum, Kagoshima and the Research Institute for Humanity and Nature, Kyoto, 70 pp.
Okiyama, M. (2001) Luciogobius adapel, a new species of gobiid fish from Japan. Bulletin of the National Museum of Nature and Science, Series A, Zoology, 27 (2), 141–149.
Sambrook, J. & Russell, D.W. (2001) Molecular Cloning: A Laboratory Manual. 3rd Edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 2100 pp.
Shibukawa, K., Aizawa, M. & Suzuki, T. (2020) Comparative morphology, validity, and limits of the genus Inu Snyder, 1909 (Gobiiformes, Oxudercidae), with comments on the diversification of the related interstice-dwelling goby genera in Japan. Bulletin of the Museum of Natural and Environmental History, Shizuoka, 13, 79–116. [in Japanese]
Shibukawa, K., Aizawa, M., Suzuki, T., Kanagawa, N. & Muto, F. (2019) Preliminary review of earthworm gobies of the genus Luciogobius (Gobiiformes, Oxudercidae) from Shizuoka Prefecture, Japan. Bulletin of the Museum of Natural and Environmental History, Shizuoka, 12, 29–96. [in Japanese]
Snyder, J.O. (1909) Descriptions of new genera and species of fishes from Japan and the Riu Kiu Islands. Proceedings of the United States National Museum, 36 (1688), 597–610. https://doi.org/10.5479/si.00963801.1688.597
Ta, T.T., Tran, H.D., Dinh, L.G., Nguyen, M.H., Tran, T.T. & Ha, L.M. (2021) Planktonic larvae of Luciogobius sp. (Gobiidae) in a tropical estuary. Regional Studies in Marine Science, 48, 102068. https://doi.org/10.1016/j.rsma.2021.102068
Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22 (22), 4673–4680. https://doi.org/10.1093/nar/22.22.4673
Yamada, T., Sugiyama, T., Tamaki, N., Kawakita, A. & Kato, M. (2009) Adaptive radiation of gobies in the interstitial habitats of gravel beaches accompanied by body elongation and excessive vertebral segmentation. BMC Evolutionary Biology, 9, 145. https://doi.org/10.1186/1471-2148-9-145