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Issue: Vol. 5154 No. 3: 15 Jun. 2022
Type: Article
Published: 2022-06-15
DOI: 10.11646/zootaxa.5154.3.10
Page range: 380-392
Abstract views: 331
PDF downloaded: 20

A new species of Tricorythodes Ulmer from Colombia and additional notes on T. serratus (Baumgardner & Ávila) (Ephemeroptera: Leptohyphidae)

Universidad de Caldas, Facultad de Ciencias Exactas y Naturales, Manizales, Colombia
2Florida A&M University, Tallahassee, Florida, United States
Universidad de Caldas, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Grupo de investigación BIONAT, Manizales, Colombia
Pannota Neotropical region new species mayfly distribution Cabecar Ephemeroptera

Abstract

A new species of Tricorythodes is described and illustrated based on nymphs from Colombia. Legs, mouthparts and other parts of the body were photographed through SEM and molecular characterization was performed by amplification of the COImt gene. Tricorythodes carare sp. nov. shows close affinities with T. serratus (Baumgardner & Ávila), both sharing a similar coloration pattern and the presence of chalazae on femora. The new species can be distinguished from T. serratus and all the species of the genus by the following combination of characters: maxillary palp 3-segmented, with apical setae; dorsal surface of median and hind femora with a longitudinal row of 5 to 9 chalazae serrated in the apex, tarsal claws with 4 or 5 marginal denticles and with 1–3 submarginal denticles; posterior margin of the abdominal terga with serrated chalazae in the apex. SEM images and distance analysis of the partial sequences of the COI gene were useful to discriminate the new species from T. serratus (genetic divergence 17–19%). An additional undescribed species of this group is reported from Esmeraldas in Ecuador and for the departments of Sucre and Chocó in Colombia, but it is not described here because of the low number of individuals and the lack of DNA integrity. What is known under the name Tricorythodes serratus is suspected to be a species complex.

References

  1. Araujo, V.A. & Dias, L.G. (2020) Tricorythopsis nupem: a new species for the Atlantic Forest from southeast Brazil (Ephemeroptera: Leptohyphidae). Zootaxa, 4885 (2), 266–270.  https://doi.org/10.11646/zootaxa.4885.2.8

    DOI: https://doi.org/10.11646/zootaxa.4885.2.8

  2. Allen, R.K. (1966) Haplohyphes, a new genus of Leptohyphinae (Ephemeroptera: Tricorythidae). Journal of the Kansas Entomological Society, 39, 565–568.

  3. Allen, R.K. & Murvosh, C.M. (1987) Mayflies (Ephemeroptera: Tricorythidae) of the southwestern United States and northern Mexico. Annals of the Entomological Society of America, 80, 35–40.  https://doi.org/10.1093/aesa/80.1.35

    DOI: https://doi.org/10.1093/aesa/80.1.35

  4. Alexander, L.C., Delion, M., Hawthorne, D.J., Lamp, W.O. & Funk, D.H. (2009) Mitochondrial lineages and DNA barcoding of closely related species in the mayfly genus Ephemerella (Ephemeroptera: Ephemerellidae). Journal of the North American Benthological Society, 28, 584–595.  https://doi.org/10.1899/08-150.1

    DOI: https://doi.org/10.1899/08-150.1

  5. Ball, S.L., Hebert, P., Burian, S.K. & Webb, J.M. (2005) Biological identifications of mayflies (Ephemeroptera) using DNA barcodes. Journal of the North American Benthological Society, 24, 508–524.  https://doi.org/10.1899/04-142.1

    DOI: https://doi.org/10.1899/04-142.1

  6. Baumgardner, D.E. & Ávila, A.S. (2006) Cabecar Serratus, a New Genus and Species of Leptohyphid Mayfly from Central America, and Description of the Imaginal Stages of Tricorythodes Sordidus Allen (Ephemeroptera: Leptohyphidae). Zootaxa, 1187 (1), 47–59. https://doi.org/10.11646/zootaxa.1187.1.3

    DOI: https://doi.org/10.11646/zootaxa.1187.1.3

  7. Dias, L.G., Bacca, T. & Ferreira, P.S. (2011) Tricorythodes caunapi: a new species from the rain forest of the Colombian pacific (Ephemeroptera: Leptohyphidae). Revista Colombiana de Entomología, 37 (2), 327–330.

    DOI: https://doi.org/10.25100/socolen.v37i2.9096

  8. Dias, L.G., Cabette, H.S. & De Sousa, D.P. (2009) A new species of Tricorythodes Ulmer, 1920 (Ephemeroptera: Leptohyphidae) and first record of Tricorythodes quizeri Molineri, 2002 from Brazil. Aquatic insects, 31, 95–99.  https://doi.org/10.1080/01650420802648858

    DOI: https://doi.org/10.1080/01650420802648858

  9. Dias, L.G., Molineri, C., Takiya, D., Benavides, P. & Bacca, T. (2019) Phylogeny of Tricorythodes Ulmer (Leptohyphidae: Ephemeroptera) based on molecular and morphological evidence. Zoologischer Anzeiger, 278, 38–45.  https://doi.org/10.1016/j.jcz.2018.10.008

    DOI: https://doi.org/10.1016/j.jcz.2018.10.008

  10. Domínguez, C., Molineri, C. & Nieto, C. (2009) Ephemeroptera. In: Domínguez, E. & Fernández, H.R. (Eds.), Macroinvertebrados Bentónicos Sudamericanos: Sistemática y Biología. Fundación Miguel Lillo, Tucumán, 44 pp.

  11. Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 5, 294–299.

  12. Gutiérrez, Y. & Dias, L.G. (2015) Ephemeroptera (Insecta) de Caldas – Colombia, claves taxonómicas para los géneros y notas sobre su distribución. Papéis Avulsos de Zoologia, 55 (2), 13–46.  https://doi.org/10.1590/0031–1049.2015.55.02

    DOI: https://doi.org/10.1590/0031-1049.2015.55.02

  13. Granados, C., Cárdenas, T., Barros, E. & Dias, L.G. (2017) Primer registro del género Cabecar (Ephemeroptera: Leptohyphidae) para el Parque Nacional Natural Tayrona. Boletín Científico Centro De Museos Museo de Historia Natural Universidad de Caldas, 21 (1), 174–178.  https://doi.org/10.17151/bccm.2017.21.1.14

  14. Kimura, M. (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of molecular evolution, 16 (2), 111–120.  https://doi.org/10.1007/BF01731581

    DOI: https://doi.org/10.1007/BF01731581

  15. Lugo-Ortiz, C.R. & McCafferty, W.P. (1995) Contribution to the taxonomy of the Leptohyphidae (Insecta: Ephemeroptera) of Central America. Studies on Neotropical Fauna and Environment, 30(3), 165–176.  https://doi.org/10.1080/01650529509360955

    DOI: https://doi.org/10.1080/01650529509360955

  16. Nei, M. & Kumar, S. (2000) Molecular evolution and phylogenetics. Oxford University Press, New York, New York, 352 pp.

  17. Oliveira, L.A., Nascimento, J.M.C.D. & Couceiro, S.R.M. (2021) A new species of Tricorythopsis Traver, 1958 (Ephemeroptera: Leptohyphidae) from Pará state, Brazil. Zootaxa, 5023 (1), 131–141.  https://doi.org/10.11646/zootaxa.5023.1.8

    DOI: https://doi.org/10.11646/zootaxa.5023.1.8

  18. Ratnasingham, S., Hebert, P.D.N. (2007) Bold: The Barcode of Life Data System (http://www.barcodinglife.org). Molecular Ecology Notes, 7, 355–364.  https://doi.org/10.1111/j.1471-8286.2007.01678.x

    DOI: https://doi.org/10.1111/j.1471-8286.2007.01678.x

  19. Salles, F.F., Domínguez, E., Molineri, E., Boldrini, R., Nieto, C. & Dias, L.G. (2018) Chapter 3—Order Ephemeroptera. In: Hamada, N., James, H., Thorp, D. & Rogers, C. (Eds.), Thorp and Covich’s Freshwater Invertebrates (Fourth Edition), Academic Press, pp. 61–117.  https://doi.org/10.1016/B978-0-12-804223-6.00003-2

    DOI: https://doi.org/10.1016/B978-0-12-804223-6.00003-2

  20. Salles, F.F., Nascimento, J.M.C., Monjardim, M., Paresque, R., Hamada, N. & Dominguez, E. (2020) Diamantina: An endemic new genus of Neotropical Atalophlebiinae (Ephemeroptera: Leptophlebiidae) evidenced by morphological and molecular data. Zoologischer Anzeiger, 284, 30–42.  https://doi.org/10.1016/j.jcz.2019.10.005

    DOI: https://doi.org/10.1016/j.jcz.2019.10.005

  21. Souto, P.M, Lima da Silveira, L.F, Maeda Takiya, D. & Salles, F.F. (2021) Cryptic diversity in the mayfly Leptohyphodes inanis (Pictet) (Ephemeroptera: Leptohyphidae) across water basins in Southeastern Brazil. Systematics and Biodiversity, 19 (7), 797–817.  https://doi.org/10.1080/14772000.2021.1933248

    DOI: https://doi.org/10.1080/14772000.2021.1933248

  22. Tamura, K., Stecher, G. & Kumar, S. (2021) MEGA11: Molecular Evolutionary Genetics Analysis version 11. Molecular Biology and Evolution, 38, 3022–3027.  https://doi.org/10.1093/molbev/msab120

    DOI: https://doi.org/10.1093/molbev/msab120

  23. Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position–specific gap penalties and weight matrix choice. Nucleic acids research, 22 (22), 4673–4680.  https://doi.org/10.1093/nar/22.22.4673

    DOI: https://doi.org/10.1093/nar/22.22.4673

  24. Ulmer, G. (1920) Neue Ephemeropteren. Archiv Fur Naturgeschichte, 85A (11), 1–80.

  25. Webb, J.M. Sun, L., McCafferty, W.P. & Ferris, V.R. (2007) A new species and new synonym in Heptagenia Walsh (Ephemeroptera: Heptageniidae: Heptageniinae) based on molecular and morphological evidence. Journal of Insect Science, 7 (1), 1–16.  https://doi.org/10.1673/031.007.6301

    DOI: https://doi.org/10.1673/031.007.6301

  26. Webb, J.M., Jacobus, L.M., Funk, D.H., Zhou, X., Kondratieff, B., Geraci, C.J., De Walt, R., Baird, D., Barton, R., Phillips, I. & Hebert, P. (2012) A DNA Barcode Library for North American Ephemeroptera: Progress and Prospects. PLOS ONE, 7(5), e38063.  https://doi.org/10.1371/journal.pone.0038063

    DOI: https://doi.org/10.1371/journal.pone.0038063

  27. Wiersema, N.A. & McCafferty, W.P. (2000) Generic Revision of the North and Central American Leptohyphidae (Ephemeroptera: Pannota). Transactions of the American Entomological Society, 126, 337–372.

  28. Williams, H.C., Osmerod, S.J. & Bruford, M.W. (2006) Molecular systematics and phylogeography of the cryptic species complex Baetis rhodani (Ephemeroptera: Baetidae). Molecular Phylogenetics and Evolution, 40, 370–382.  https://doi.org/10.1016/j.ympev.2006.03.004

    DOI: https://doi.org/10.1016/j.ympev.2006.03.004