Abstract
In this study, two new species of Setostylus (Diptera: Keroplatidae: Keroplatinae), S. tridigitus sp. n. and S. triumphus sp. n. are described, with a key to all the species of the genus. Male habitus and images of diagnostic morphological characteristics are provided. Status of these two new species is also supported by the genetic distances and neighbor-joining (NJ) tree in the DNA barcode analysis.
References
Edwards, F.W. (1931) Fauna Sumatrensis. Mycetophilidae (Diptera). Tijdshrift voor Entomologie, 74, 262–278.
Evenhuis, N.L. (2006) Catalog of the Keroplatidae of the world (Insecta: Diptera). Bishop Museum Bulletin in Entomology, 13, 1–178.
Hebert, P.D., Penton, E.H., Burns, J.M., Janzen, D.H. & Hallwachs, W. (2004) Ten species in one: DNA barcoding reveals cryptic species in the Neotropical skipper butterfly Astraptes fulgerator. Proceedings of the National Academy of Sciences, 101 (41), 14812–14817. https://doi.org/10.1073/pnas.0406166101
Hebert, P.D., Braukmann, T.W., Prosser, S.W., Ratnasingham, S., DeWaard, J.R., Ivanova, N.V., Janzen, D.H., Hallwachs, W., Naik, S., Sones, J.E. & Zakharov, E.V. (2018) A Sequel to Sanger: amplicon sequencing that scales. BMC genomics, 19 (1), 1–14. https://doi.org/10.1186/s12864-018-4611-3
Huerta, H. & Fitzgerald, S.J. (2020) A new species of Setostylus Matile and new records of Setostylus bellulus (Williston) (Diptera: Keroplatidae). Zootaxa, 4722 (1), 84–90. https://doi.org/10.11646/zootaxa.4722.1.8
Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35 (6), 1547–1549. https://doi.org/10.1093/molbev/msy096
Lane, J. (1948) Ceroplatinae da Região Neotropical (Diptera, Mycetophilidae). Revista Brasileira de Entomologia, 19 (3), 437–458.
Matile, L. (1990) Recherches sur la systématique et l’évolution des Keroplatidae (Diptera, Mycetophilidae). Memoires du Museum National d’Histoire Naturelle, Series A, 148, 1–682.
Mantič, M., Sikora, T., Burdíková, N., Blagoderov, V., Kjærandsen, J., Kurina, O. & Ševčík, J. (2020) Hidden in plain sight: comprehensive molecular phylogeny of Keroplatidae and Lygistorrhinidae (Diptera) reveals parallel evolution and leads to a revised family classification. Insects, 11 (6), 348. https://doi.org/10.3390/insects11060348
Papp, L., Merz, B. & Földvári, M. (2006) Diptera of Thailand. A summary of the families and genera with references to the species representations. Acta Zoologica Academiae Scientiarum Hungaricae, 52 (2), 97–269.
Sasakawa, M. & Tamu, N. (1961) Japanese Fungivoridae (Diptera) III: New or Little-known fungus gnats from the Tsushima Islands (Agriculture). The scientific reports of Kyoto Prefectural University. Agriculture, 13, 68–69.
Ševčík, J., Krzemiński, W. & Skibińska, K. (2020) Intriguing and Beautiful: Adamacrocera adami gen. et sp. nov. from the Upper Cretaceous Amber of Myanmar Represents a New Subfamily of Keroplatidae (Diptera: Bibionomorpha). Insects, 11 (9), 552. https://doi.org/10.3390/insects11090552
Ševčík, J., Hippa, H. & Burdíková, N. (2021) Just a Fragment of Undescribed Diversity: Twenty New Oriental and Palearctic Species of Sciaroidea (Diptera), including DNA Sequence Data and Two New Fossil Genera. Insects, 13 (1), 19. https://doi.org/10.3390/insects13010019
Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22 (22), 4673–80. https://doi.org/10.1093/nar/22.22.4673
Wang, Q.Y., Qi, L., Wu, H. & Huang, J.H. (2021) Two new species of Heteropterna Skuse, 1888 (Diptera: Keroplatidae) from China. Zootaxa, 4985 (2), 277–284. https://doi.org/10.11646/zootaxa.4985.2.11
Xu, H.C., Cao, J., Zhou, Z.J., Wu, H. & Huang, Z.W. (2007) First record of the tribe Keroplatini from China, with descriptions of two new species (Diptera: Keroplatidae). Zootaxa, 1497 (1), 35–40. https://doi.org/10.11646/zootaxa.1497.1.3