Abstract
Cytochrome b (Cytb) sequences from 23 specimens of Mastacembelus spp. from the Rokel River, Taia/Jong River basins, and Freetown Peninsula were used in this study. These sequences were used to perform a gene-based phylogenetic analysis along with closely allied congeneric species in the Upper Guinea Forest in Africa. The resulting phylogenies inferred from Cytb showed populations of M. liberiensis from the Rokel River basin clustered into an exclusive lineage being sister to the lineage formed by M. greshoffi from the Congo basin. The Freetown Peninsula population of M. liberiensis clustered with sequences previously identified as M. taiaensis from the Taia/Jong River basin, sister to sequences of M. taiaensis from the Rokel River basin. The paired lineages were sister to the lineage formed by sequences from samples of the Taia/Jong River basin. These findings lead to the following conclusions: (1) hidden species diversity of spiny eels exists in either the Rokel River or the Jong River basin; (2) a likely available name for this species from the Freetown Peninsula is M. laticauda distinct from M. liberiensis; (3) given the diversity within M. liberiensis observed in the rivers sampled in Sierra Leone, it seems likely that the remaining rivers of Liberia and Sierra Leone reported having M. liberiensis may also harbor undescribed diversity. It is difficult to elevate some available junior synonyms based solely on a phylogeny inferred from Cytochrome b. We suggest that more samples from the entire range of M. liberiensis are needed and an integrated approach is required.
References
Ahl, E. (1937) Ueber eine kleine Fischsammmlung a us Sierra Leone. Mitteilungen aus dem Zoologischen Museum in Berlin, 22, 239–243. https://doi.org/10.1002/mmnz.19370220208
Alter, S.E., Brown, B. & Stiassny, M.L. (2015) Molecular phylogenetics reveals convergent evolution in lower Congo River spiny eels. BMC Evolutionary Biology, 15 (1), 1–12. https://doi.org/10.1186/s12862-015-0507-x
Beshera, K.A., Harris, P.M. & Mayden, R.L. (2016) Novel evolutionary lineages in Labeobarbus (Cypriniformes; Cyprinidae) based on phylogenetic analyses of mtDNA sequences. Zootaxa, 4093 (3), 363–381 https://doi.org/10.11646/zootaxa.4093.3.4
Brown, K.J., Rüber, L., Bills, R. & Day, J.J. (2010) Mastacembelid eels support lake tanganyika as an evolutionary hotspot of diversification. BMC Evolutionary Biology, 10, 188. https://doi.org/10.1186/1471-2148-10-188
Brown, K.J., Britz, R., Bills, R., Rüber, L. & Day, J.J. (2011) Pectoral fin loss in the Mastacembelidae: A new species from Lake Tanganyika. Journal of Zoology, 284 (4), 286–293. https://doi.org/10.1111/j.1469-7998.2011.00804.x
Day, J.J., Fages, A., Brown, K.J., Vreven, E.J., Stiassny, M.L., Bills, R., Friel, J.P. & Rüber, L. (2017) Multiple independent colonizations into the Congo Basin during the continental radiation of African Mastacembelus spiny eels. Journal of Biogeography, 44 (10), 2308–2318. https://doi.org/10.1111/jbi.13037
Darriba, D., Posada, D., Kozlov, A.M., Stamatakis, A., Morel, B. & Flouri, T. (2020) ModelTest-NG: A New and Scalable Tool for the Selection of DNA and Protein Evolutionary Models. Molecular Biology and Evolution, 37 (1), 291–294. https://doi.org/10.1093/molbev/msz189
Fricke, R., Eschmeyer, W., Van Der laan, R. & Utrecht, H. (2021) Catalog of Fishes : Genera , Species , References. September.
Ivanova, N.V., Zemlak T.S., Hanner, R.H. & Hebert, P.D.N. (2007) Universal primer cocktails for fish DNA barcoding. Molecular Ecology Notes. Molecular Ecology Notes, 7 (4), 544–548. https://doi.org/10.1098/rstb.2005.1716
Kanu, U.C., Liang, C., Nwafor, C.C., Shen, J. & Zhang, E. (2022) Phylogenetic Analyses of Cyprinid Species from the Rokel River Basin of Sierra Leone, West Africa: Taxonomic, Biogeographic, and Conservation Implications. Diversity, 14 (4), 299. https://doi.org/10.3390/d14040299
Kimura, M. (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16 (2), 111–120. https://doi.org/10.1007/BF01731581
Kottelat, M. (2006) The Fishes of the Inland Waters of Southeast Asia: A Catalogue and Core Bibliography of the Fishes Known to Occur in Freshwaters, Mangroves and Estuaries Maurice. Raffles Bulletin of Zoology, 54 (2).
Kottelat, M. (2010) Ichthyological Exploration of Freshwaters. Ichthyological Exploration of Freshwaters, 24 (2).
Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Molecular Biology and Evolution, 33 (7), 1870–1874. https://doi.org/10.1093/molbev/msw054
Lakner, C., Van Der Mark, P., Huelsenbeck, J.P., Larget, B. & Ronquist, F. (2008) Efficiency of markov chain monte carlo tree proposals in bayesian phylogenetics. Systematic Biology, 57 (1), 86–103. https://doi.org/10.1080/10635150801886156
Lalèyè, P., Olaosebikan, D.B., Dankwa, H., Schmidt, R.C., Diallo, I. & Darwall, W.R. (2021) The status and distribution of freshwater fishes in western Africa. Identification and Validation of Western African Freshwater Key Biodiversity Areas, 22.
Leveque, C. (1999) Fishes of African continental waters: diversity, ecology, human activities. IRD Editions, Paris (France), 521 pp. [https://www.researchgate.net/publication/282169802%0ARichesse]
Librado, P. & Rozas, J. (2009) DnaSP v5: A software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25 (11), 1451–1452. https://doi.org/10.1093/bioinformatics/btp187
Nelson, J.S., Grande, T.C. & Wilson, M.V.H. (2016) Fisshes of the World 5th edition. John Wiley & Sons, Hoboken, New Jersey, 707 pp. https://doi.org/10.1002/9781119174844
Paugy, D., Leveque, C., Teugels, G.G., Bigorne, R. & Romand, R. (1990) Freshwater Fishes of Sierra Leone and Liberia Annotated Checklist and Distribution. Revue d’Hydrobiologie Tropicale, 23 (1), 329–350.
Paugy, D., Lévêque, C. & Teugels, G.G. (2003) The Fresh and Brackish Water Fishes of West Africa, vols 1 & 2. Muséum national d'Histoire naturelle, Paris, 1272 p. [Faune et Flore tropicales; 40]
Payne, I.A. (2018) The ecology, distribution and diversity of fish species in Sierra Leone rivers and response to human impacts. Environmental Biology of Fishes, 101 (5), 843–864. https://doi.org/10.1007/s10641-018-0742-2
Payne, A., Ndomahina, T.E. & Wakeford, R.C. (2010) Fish distribution and zonation along a tropical African river, the Rokel/Seli River, Sierra Leone, West Africa. Smithiana Bulletin 12, 25–36.
Roberts, R. (1986) Systematic Thailand, Review of the Mastacembelidae or Spiny Eels of Burma and with Description of Two New Species of Macrognathus. Japanese Journal of Ichthyology, 33 (2), 95–109. https://doi.org/10.1007/BF02905838
Roberts, T.R. (1975) Geographical distribution of African freshwater fishes. Zoological Journal of the Linnean Society, 57 (4), 249–319. https://doi.org/10.1111/j.1096-3642.1975.tb01893.x
Stamatakis, A. (2014) RAxML version 8: A tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics, 30 (9), 1312–1313. https://doi.org/10.1093/bioinformatics/btu033
Schmidt, R.C. & Pezold, F. (2011) Morphometric and molecular variation in mountain catfishes (Amphiliidae: Amphilius) in Guinea, West Africa. Journal of Natural History, 45 (9-10), 521–552. https://doi.org/10.1080/00222933.2010.534560
Schmidt, R.C., Bart Jr, H.L. & Pezold, F. (2016) High levels of endemism in suckermouth catfishes (Mochokidae: Chiloglanis) from the Upper Guinean forests of West Africa. Molecular Phylogenetics and evolution, 100, 199–205. https://doi.org/10.1016/j.ympev.2016.04.018
Schmidt, R.C., Bart Jr, H.L., Pezold, F. & Friel, J.P. (2017b) A biodiversity hotspot heats up: Nine new species of suckermouthcatfishes (Mochokidae: Chiloglanis) from Upper Guinean forest streams in West Africa. Copeia, 105 (2), 301–338. https://doi.org/10.1643/CI-16-474
Travers, R.A. (1992) Caecomastacembelus taiaensis and Aethiomastacembelus praensis, two new species of mastacembelid spiny-eels from West Africa. 2 (4), 331–340.
Van Steenberge, M., Vreven, E.J. & Snoeks, J. (2014) The fishes of the Upper Luapula area (Congo basin): a fauna of mixed origin. Ichthyological Exploration of Freshwaters, 24 (4), 289–384.
Vreven, E.J. & Teugels, G.G. (2005) Redescription of Mastacembelus liberiensis Boulenger, 1898 and description of a new West African spiny-eel (Synbranchiformes: Mastacembelidae) from the Konkoure River basin, Guinea. Journal of Fish Biology, 67 (2), 332–369. https://doi.org/10.1111/j.0022-1112.2005.00729.x
Vreven, E.J. & Teugels, G.G. (1996) Description of a new Mastacembelid species (Synbranchiformes; Mastacembelidae) from the Zaire River Basin in Africa Copeia, 130–139. https://doi.org/10.2307/1446948
Vreven, E.J & Stiassny, M.L.J. (2009) Mastacembelus simbi , a new dwarf spiny eel (Synbranchiformes: Mastacembelidae) from the lower Congo River. Ichthyological Exploration of Freshwaters, 20 (3), 213–222.
Xia, X. (2013) DAMBE5: A comprehensive software package for data analysis in molecular biology and evolution. Molecular Biology and Evolution, 30 (7), 1720–1728. https://doi.org/10.1093/molbev/mst064
Zhang, D., Gao, F., Jakovlić, I., Zou, H., Zhang, J., Li, W.X. & Wang, G.T. (2020) PhyloSuite: An integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies. Molecular Ecology Resources, 20 (1), 348–355. https://doi.org/10.1111/1755-0998.13096