Abstract
We studied the morphological and genetic differences within Calotes jerdoni, a widespread species across northeast India. Our results suggest the presence of two distinct species in this region, one being Calotes jerdoni and the other being Calotes medogensis, which we report for the first time from India. We designate a lectotype for Calotes jerdoni and provided extended description based on freshly collected materials. Previously undetermined diagnostic characters were identified and are discussed here in detail. The aforementioned species show an interspecific pairwise genetic divergence of 13–14% in the ND2 mitochondrial gene.
References
Ahmed, M. ., Das, A. & Dutta, S.K. (2009) Amphibians and Reptiles of Northeast India: A photographic guide. Aaranyak, Guwahati, 169 pp.
Annandale, N. (1912) Zoological results of the Abor Expedition, 1911–12. Batrachia and Reptilia. Records of the Indian Museum, 8, 7–59.
Benson, D.A., Karsch-Mizrachi, I., Lipman, D.J., Ostell, J. & Wheeler, D.L. (2007) GenBank. Nucleic acids research, 35 (Supplement 1), D21–D25. https://doi.org/10.1093/nar/gkl986
Che, J., Jiang, K., Yan, F. & Zhang, Y. (2020) Amphibians and Reptiles in Tibet—Diversity and Evolution. Chinese Academy of Sciences. Science Press, Beijing, 803 pp.
Das, A., Saikia, U., Murthy, B.H.C.K., Dey, S. & Dutta, S.K. (2009) A herpetofaunal inventory of Barail Wildlife Sanctuary and adjacent regions, Assam, north-eastern India. Hamadryad, 34 (1), 117–134.
Das, A., Sharma, P., Surendran, S., Nath, A., Ghosh, S., Dutta, D., Mondol, J. & Wngdi, Y. (2016) Addition to the herpetofauna of Royal Manas National Park, Bhutan, with six new country records. Herpetology Notes, 9, 261–278.
Decemson, H.T., Vabeiryureilai, M., Lalbiakzuala, L. & Lalremsanga, H.T. (2021) Confirmation on the occurrence of Calotes geissleri Wagner, Ihlow, Hartman, Flecks, Schmitz and Böhme, 2021 (Sauria: Agamidae) in Chandel, Manipur, India with comments on its phylogenetic position. Journal of Animal Diversity, 3 (4), 14–19. https://doi.org/10.52547/JAD.2021.3.4.2
Deepak, V., Vyas, R., Giri, V.B. & Karanth, P. (2015) A taxonomic mystery for more than 180 years: the identity and systematic position of Brachysaura minor (Hardwicke & Gray, 1827). Vertebrate zoology, 65 (3), 371–381.
Giri, V.B., Chaitanya, R., Mahony, S., Lalrounga, S., Lalrinchhana, C., Das, A., Sarkar, V., Karanth, P. & Deepak, V. (2019) On the systematic status of the genus Oriocalotes Günther, 1864 (Squamata: Agamidae: Draconinae) with the description of a new species from Mizoram state, Northeast India. Zootaxa, 4638 (4), 451–484.
https://doi.org/10.11646/zootaxa.4638.4.1
Gowande, G., Pal, S., Jablonski, D., Masroor, R., Phansalkar, P.U., Dsouza, P., Jayarajan, A. & Shanker, K. (2021) Molecular phylogenetics and taxonomic reassessment of the widespread agamid lizard Calotes versicolor (Daudin, 1802) (Squamata, Agamidae) across South Asia. Vertebrate Zoology, 71, 669–696. https://doi.org/10.3897/vz.71.e62787
Gray, J.E. (1845) Catalogue of the Specimens of Lizards in the Collection of the British Museum. order of the Trustees, London, 289 pp.
Günther, A. (1870) Descriptions of a new Indian lizard of the genus Calotes. Proceedings of Zoological Society of London, 1870, 778–779.
Jangid, A.K., Kumar, G.C., Singh, C.P. & Böhm, M. (2021) Potential distribution of the endemic Short-tailed ground agama Calotes minor (Hardwicke & Gray, 1827) in drylands of the Indian sub-continent. Herpetological Journal, 31 (3), 132–141. https://doi.org/10.33256/31.3.132141
Kalyaanamoorthy, S., Minh, B.Q., Wong, T.K.F., von Haeseler, A. & Jermiin, L.S. (2017) ModelFinder: Fast model selection for accurate phylogenetic estimates. Nature Methods, 14, 587–589. https://doi.org/10.1038/nmeth.4285
Krishnan, S. (2008) New Species of Calotes (Reptilia: Squamata: Agamidae) from the Southern Western Ghats, India. Journal of Herpetology, 42 (3), 530–535. https://doi.org/10.1670/06-198.1
Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33, 1870–1874. https://doi.org/10.1093/molbev/msw054
Lalremsanga, H.T., Khawlhring, L. & Lalrotluanga (2010) Three additional lizard (Squamata: Sauria) records for Mizoram, India. Journal of Threatened Taxa, 2 (2), 718–720.
Lajmi, A., Datta-Roy, A., Vyas, R., Agarwal, I. & Karanth, P. (2018) The curious case of Hemidactylus gujaratensis (Squamata: Gekkonidae). Zootaxa, 4388 (1), 137–142. https://doi.org/10.11646/zootaxa.4388.1.11
Lanfear, R., Frandsen, P.B., Wright, A.M., Senfeld, T. & Calcott, B. (2017) PartitionFinder 2: new methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Molecular Biology and Evolution, 34 (3), 772–773. https://doi.org/10.1093/molbev/msw260
Liu, S., Zuo, C. & Rao, D. (2021) Distribution extension of Calotes irawadi Zug, Brown, Schulte & Vindum, 2006, previously confused with C. versicolor (Daudin, 1802): first record from China. Herpetozoa, 34, 83–88. https://doi.org/10.3897/herpetozoa.34.e62596
Macey, R.J., Larson, A., Ananjeva, N.B., Fang, Z. & Papenfuss, T. (1997) Two novel gene orders and the role of light-strand replication in rearrangement of the vertebrate mitochondrial genome. Molecular Biology and Evolution, 14, 91–104. https://doi.org/10.1093/oxfordjournals.molbev.a025706
Minh, B.Q., Nguyen, M.A.T. & von Haeseler, A. (2013) Ultrafast Approximation for Phylogenetic Bootstrap. Molecular Biology and Evolution, 30 (5), 1188–1195. https://doi.org/10.1093/molbev/mst024
Nguyen, L.T., Schmidt, H.A., Von Haeseler, A. & Minh, B.Q. (2015) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular biology and evolution, 32 (1), 268–274. https://doi.org/10.1093/molbev/msu300
Pal, S., Vijayakumar, S. P., Shanker, K., Jayarajan, A. & Deepak, V. (2018) A systematic revision of Calotes Cuvier, 1817 (Squamata: Agamidae) from the Western Ghats adds two genera and reveals two new species. Zootaxa, 4482 (3), 401–450. https://doi.org/10.11646/zootaxa.4482.3.1
Pawar, S. & Birand, A. (2001) A survey of amphibians, reptiles, and birds in Northeast India. CERC Technical Report 6. Centre for Ecological Research and Conservation, Mysore, 118 pp.
Rambaut, A., Suchard, M.A., Xie, D. & Drummond, A.J. (2014) Tracer. Version 1.6. Available from: http://beast.bio.ed.ac.uk/Tracer (accessed 22 November 2022)
Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D., Darling, A., Höhna, S., Larget B., Liu L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61, 539–542. https://doi.org/10.1093/sysbio/sys029
Sanyal, D.P. & Gayen, N.C. (2006) Reptilia. In: Alfred, J.R.B. (Ed.), Fauna of Arunachal Pradesh. State Fauna Series. Zoological Survey of India, Kolkata, pp. 247–284.
Sengupta, D., Borah, C.G. & Phukon, J. (2019) Assessment of the reptilian fauna in the Brahmaputra Plains of two districts in Assam, India. Reptiles & Amphibians, 26 (1), 65–67.
Sinha, B., Nath, K.P. & Gurumayum, S.D. (2021) Herpetofaunal Diversity of Zoological Survey of India Campus, Itanagar, Arunachal Pradesh, India. Records of the Zoological Survey of India, 121 (3), 411–418.
Smith, M.A. (1943) The Fauna of British India, Ceylon, and Burma, Including the Whole of the Indo-Chinese Sub-region: Reptilia and Amphibia. Vol. III. Serpentes. Taylor And Francis, London, 583 pp.
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. (1997) The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25, 4876–4882. https://doi.org/10.1093/nar/25.24.4876
Trifinopoulos, J., Nguyen, L.T., von Haeseler, A. & Minh, B.Q. (2016) W-IQ-TREE: A fast online phylogenetic tool for Maximum likelihood analysis. Nucleic Acids Research, 44, W232–W235. https://doi.org/10.1093/nar/gkw256
Uetz, P., Freed, P. & Hošek, J. (Eds.) (2022) The Reptile Database. Available from: http://www.reptile-database.org (accessed 5 June 2022)
Wagner, P., Ihlow, F., Hartmann, T., Flecks, M., Schmitz, A. & Böhme, W. (2021) Integrative approach to resolve the Calotes mystaceus Duméril & Bibron, 1837 species complex (Squamata: Agamidae). Bonn Zoological Bulletin, 70 (1), 141–171. https://doi.org/10.20363/BZB-2021.70.1.141
Zhao, E. & Li, S. (1984) A new species of Calotes (Lacertilia: Agamidae) from Xizang (Tibet). Acta herpetological sinica, 3 (4), 77–78.
Zug, G.R., Brown, H.H., Schulte, J.A. & Vindum, J.V. (2006) Systematics of the garden lizards, Calotes versicolor group (Reptilia, Squamata, Agamidae), in Myanmar: Central dry zone populations. Proceedings of the California Academy of Sciences, 57 (2), 35–68.