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Type: Article
Published: 2008-06-06
Page range: 11–38
Abstract views: 117
PDF downloaded: 44

Three new species of leaflitter frogs from the upper Amazon forests: cryptic diversity within Pristimantis "ockendeni" (Anura: Strabomantidae) in Ecuador

Department of Biology, Queen’s University, Kingston ON K7L 3N6 Canada Lehrstuhl für Zoologie und Evolutionsbiologie, Department of Biology, University of Konstanz, Universitätstraβe 10, 78457 Konstanz, Germany
Section of Integrative Biology and Texas Memorial Museum, The University of Texas, Austin, TX 78712, USA
Amphibia Anura new species terrestrial frog South America Pristimantis achuar Pristimantis altamnis Pristimantis kichwarum

Abstract

We describe three new species of Pristimantis leaflitter frogs from the upper Amazon basin of Ecuador: Pristimantis achuar, Pristimantis altamnis, and Pristimantis kichwarum. Each of these new species was previously considered to be part of a single species, formerly known as Eleutherodactylus ockendeni (Boulenger) morph B. However, analysis of DNA sequences together with examination of the morphology of more than 600 specimens from Ecuador reveals that multiple species were confused under the same name. The morphological differences among the three new species are subtle but consistent: presence or absence of a black canthal stripe and/or supratympanic ridge, snout-vent length, and ventral colouration. The three species are generally allopatric and geographically restricted, although at some localities two of the species occur sympatrically.Describimos tres especies nuevas de ranas del sotobosque de la cuenca Amazónica Ecuatoriana: Pristimantis achuar, Pristimantis altamnis y Pristimantis kichwarum. Estas especies fueron consideradas antes como una sola especie bajo el nombre Eleutherodactylus ockendeni (Boulenger) morfo B. Sin embargo, el análisis de secuencias de ADN y la examinación de la morfología de más que 600 especímenes Ecuatorianos indican que en realidad existen tres especies. Sus diferencias morfológicas son sutiles pero constantes: presencia o ausencia de una raya cantal negra y/o de un pliegue supratimpánico y su coloración ventral. Las tres especies tienen distribuciones alopátricas y distintas aunque en algunas localidades se puede encontrar dos de las tres especies juntas.

References

  1. Acosta-Galvis, A.R. (2000) Ranas, salamandras, y caecilias (Tetrapoda: Amphibia) de Colombia. Biota Colombiana, 1, 289–319.

    Aichinger, M. (1987) Annual activity patterns of anurans in a seasonal neotropical environment. Oecologia, 71, 583–592.

    Andersson, L.G. (1945) Batrachians From East Ecuador Collected 1937, 1938 By Wm. Clarke-Macintyre and Rolf Blomberg. Kongliga Svenska Vetenskaps-Akademiens Stockholm, 1–88 pp.

    Bokermann, Werner C. A. (1958) A preoccupied name of a neotropical frog, genus Eleutherodactylus. Herpetologica, 14, 95.

    Boulenger, G.A. (1912) Descriptions of new Batrachians from the Andes of South America, preserved in the British Museum. Annals and Magazine of Natural History, ser 8, X, 185–191.

    Camargo, A., de Sá, R.O. & Heyer, W.R. (2006) Phylogenetic analyses of mtDNA sequences reveal three cryptic lineages in the widespread neotropical frog Leptodactylus fuscus (Schneider, 1799) (Anura, Leptodactylidae). Biological Journal of the Linnean Society, 87, 325–341.

    Cisneros-Heredia, D.F. (2003). Herpetofauna de la Estación de Biodiversidad Tiputini, Amazonía Ecuatoriana: Ecología de una comunidad taxonómicamente diversa con comentarios sobre methodologías de inventario. In S. De la Torre, & G. Reck (Eds.), (CD). Quito, Ecuador: Universidad San Francisco de Quito.

    Cisneros-Heredia, D.F. (2005) Amphibia, Epipedobates bilinguis, Agalychnis calcarifer, Eleutherodactylus croceoinguinis: Distribution extensions. Check List, 1, 14–15.

    Cisneros-Heredia, D.F. (2006) Notes on Geographic Distribution: Amphibia, Brachycephalidae, Eleutherodactylus skydmainos: First country record, Ecuador. Check List, 2, 47–49.

    Chek, A.A., Lougheed, S.C., Bogart, J.P. & Boag, P.T. (2001) Perception and history: molecular phylogeny of a diverse group of neotropical frogs, the 30-chromosome Hyla (Anura: Hylidae). Molecular Phylogenetics and Evolution, 18, 370–85.

    Coloma, L.A. (ed) (2005–2007) Anfibios de Ecuador [en linea]. Ver. 2.0 (29 Oct. 2005). Museo de Zoologia, Pontificia Universidad Catolica del Ecuador Quito, Ecuador. <www.puce.edu.ec/zoologia/vertebrados/amphibiawebec/anfibiosecuador/index.html>.

    Crawford, A.J. (2003) Huge populations and old species of Costa Rican and Panamanian dirt frogs inferred from mitochondrial and nuclear gene sequences. Molecular Ecology, 12, 2525–2540.

    Crawford A.J., Bermingham E. & Polanía S.C. (2007) The role of tropical dry forest as a long-term barrier to dispersal: a comparative phylogeographical analysis of dry forest tolerant and intolerant frogs. Molecular Ecology, 16, 4789–4807.

    Doan, T.M. & Arizábal Arriaga, W. (2002) Microgeographic variation in species composition of the herpetofaunal communities of Tambopata region, Peru. Biotropica, 34, 101–117.

    Duellman, W.E. (1978) The Biology of an Equatorial Herpetofauna in Amazonian Ecuador. University of Kansas Lawrence, Kansas, USA, 1–352.

    Duellman, W.E. (2005) Cusco Amazónico. The lives of amphibians and reptiles in an Amazonian rainforest. Cornell University Press.

    Duellman, W.E. & Lynch, J.D. (1988). Anuran amphibians from the cordillera de Cutucú. Proceedings of the Academy of Natural Sciences of Philadelphia, 140, 125–142.

    Duellman, W.E. & Mendelson III, J.R. (1995) Amphibians and reptiles from northern Departamento Loreto, Peru: taxonomy and biogeography. University of Kansas Science Bulletin, 55, 329–376.

    Duellman, W.E. & Pramuk, J.B. (1999) Frogs of the genus Eleutherodactylus (Anura: Leptodactylidae) in the Andes of northern Peru. Natural History Museum of the University of Kansas, Miscellaneous Publication, 13, 1–78.

    Duellman, W.E. & Salas, A.W. (1991) Annotated checklist of the amphibians and reptiles of Cuzco Amazonica, Peru. Occasional Papers of the Museum of Natural History, Univ. of Kansas, 143, 1–13.

    Duellman, W.E. & Thomas, R. (1996) Anuran amphibians from a seasonally dry forest in southeastern Peru and comparison of the anurans among sites in the upper Amazon basin. Occasional Papers of the Natural History Museum University of Kansas, 180, 1–34.

    Elmer, K.R. (2004) Geographic distribution. Eleutherodactylus librarius. Herpetological Review, 35, 77.

    Elmer, K.R., Dávila, J.A. & Lougheed, S.C. (2006) Isolation of simple and compound polymorphic tetranucleotide microsatellites for the neotropical leaflitter frog Eleutherodactylus ockendeni (Leptodactylidae). Molecular Ecology Notes, 6, 891–893.

    Elmer, K.R., Dávila, J.A. & Lougheed, S.C. (2007a) Applying new inter-individual approaches to assess fine-scale population genetic diversity in a neotropical frog, Eleutherodactylus ockendeni. Heredity, 99, 506–515.

    Elmer, K.R., Dávila, J.A. & Lougheed, S.C. (2007b) Cryptic diversity and deep divergence in an upper Amazonian leaflitter frog, Eleutherodactylus ockendeni. BMC Evolutionary Biology, 7, 247.

    Flores, G. & Vigle, G.O. (1994) A new species of Eleutherodactylus (Anura: Leptodactylidae) from the lowland rainforests of Amazonian Ecuador, with notes on the Eleutherodactylus frater assembly. Journal of Herpetology, 28, 416–424.

    Fouquet, A., Vences, M., Salducci, M.-D., Meyer, A., Marty, C., Blanc, M. & Gilles, A. (2007) Revealing cryptic diversity using molecular phylogenetics and phylogeography in frogs of the Scinax ruber and Rhinella margaritifer species groups. Molecular Phylogenetics and Evolution, 43, 567–582.

    Graham, C.H., Ron, S.R., Santos, J.C., Schneider, C.J. & Moritz, C. (2004) Integrating phylogenetics and environmental niche models to explore speciation mechanisms in dendrobatid frogs. Evolution, 58, 1792–1793.

    Guayasamín, J.M., Ron, S.R., Cisneros-Heredia, D.F., Lamar, W. & McCracken, S.F. (2006) A new species of frog of the Eleutherodactylus lacrimosus assemblage (Leptodactylidae) from the western Amazon basin, with comments on the utility of canopy surveys in lowland rainforest. Herpetologica, 62, 191–202.

    Hedges, S. B., Duellman, W. E. & Heinicke, M.P. (2008) New World direct-developing frogs (Anura: Terrarana): molecular phylogeny, classification, biogeography, and conservation. Zootaxa, 1737, 1–182.

    Heinicke, M.P., Duellman, W.E. & Hedges, S.B. (2007) Major Caribbean and Central American frog faunas originated by ancient oceanic dispersal. Proceedings of the National Academy of Sciences of the United States of America, 104, 10092–10097.

    Heyer, W.R. (1988) On frog distribution patterns east of the Andes. In: Heyer, W.R. & P.E. Vanzolini (Eds.). Proceedings of a Workshop on Neotropical Distribution Patterns. Acad. Brasil. Cien. Rio de Janeiro, Brazil, pp. 245–273.

    Heyer, W.R. & Berven, K.A. (1973) Species diversities of herpetofaunal samples from similar microhabitats at two tropical sites. Ecology, 54, 642–645.

    IUCN, Conservation International & NatureServe. (2006). Global Amphibian Assessment. <www.globalamphibians.org>Izquierdo, J., Nogales, F. & Yánez, A.P. (2000) Análisis herpetofaunístico de un bosque húmedo tropical en la Amazonía Ecuatoriana. Ecotrópicos, 13, 29–42.

    Lehr, E., Torres, C. & Suárez, J. (2007) A new species of arboreal Eleutherodactylus (Anura: Leptodactylidae) from the Amazonian lowlands of central Peru. Herpetologica, 63, 94–99.

    Lougheed, S.C., Gascon, C., Jones, D.A., Bogart, J.P. & Boag, P.T. (1999) Ridges and rivers: a test of competing hypotheses of Amazonian diversification using a dart-poison frog (Epipedobates femoralis). Proceedings of the Royal Society of London. Series B, 266, 1829–1835.

    Lynch, J.D. (1968) Systematic status of some Andean leptodactylid frogs with a description of a new species of Eleutherodactylus. Herpetologica, 24, 289–300.

    Lynch, J.D. (1974) New species of frogs (Leptodactylidae: Eleutherodactylus) from the Amazonian lowlands of Ecuador. Occasional Papers of the Museum of Natural History Kansas, 31, 1–22.

    Lynch, J.D. (1980) A taxonomic and distributional synopsis of the Amazonian frogs of the genus Eleutherodactylus. American Museum Novitates, 2696, 1–24.

    Lynch, J. D. & Duellman, W. E. (1980) The Eleutherodactylus of the Amazonian slopes of the Ecuadorian Andes (Anura: Leptodactylidae). Miscellaneous Publications of the Natural History Museum, University of Kansas, 69, 1–86.

    Lynch, J.D. & Duellman, W.E. (1997) Frogs of the genus Eleutherodactylus (Leptodactylidae) in western Ecuador: systematics, ecology, and biogeography. Miscellaneous Publications of the Natural History Museum, University of Kansas, 23, 1–236.

    Maddison, D.R. & Maddison, W.P. (2003) MacClade 4: Analysis of Phylogeny and Character Evolution. Version 4.07. Sinauer Associates, Sunderland, USA.

    Moritz C. & Cicero C. (2004) DNA Barcoding: Promise and Pitfalls. PLoS Biology, 2, e354.

    Padial, J.M., Gonzáles, L., Reichle, S., Aguayo, R. & de la Riva, I. (2004) First records of five species of the genus Eleutherodactylus Duméril and Bibron, 1841 (Anura, Leptodactylidae) for Bolivia. Graellsia, 60, 167–174.

    Pearman, P.B. (1997) Correlates of amphibian diversity in an altered landscape in Amazonian Ecuador. Conservation Biology, 11, 1211–1225.

    Posada, D (1998–2006) Collapse: Describing Haplotypes From Sequence Alignments Ver. 1.2. <http://darwin.uvigo.es/software/collapse.html>.

    Rodríguez, L.O. & Duellman, W.D. (1994) Guide to Frogs of the Iquitos Region, Amazonian Peru. University of Kansas Museum of Natural History Special Publication No. 22 Kansas, USA, 1–80 pp.

    Ron, S.R., Santos, J.C. & Cannatella, D.C. (2006) Phylogeny of the tungara frog genus Engystomops (=Physalaemus pustulosus species group; Anura: Leptodactylidae). Molecular Phylogenetics and Evolution, 39, 392–403.

    Swofford, D.L. (2003) Paup*. Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4. Sinauer Associates, Sunderland, USA.

    Toft, C.A. (1980) Feeding ecology of thirteen syntopic species of anurans in a seasonal tropical environment. Oecologia, 45, 131–141.

    Toft, C.A. & Duellman, W.E. (1979) Anurans of the lower Rio Llulapichis, Amazonian Peru: a preliminary analysis of community structure. Herpetologica, 35, 71–77

    Vences, M., Thomas, M., van der Meijden, A., Chiari, Y. & Vietes, D. R. (2004). Comparative performance of the 16S rRNA gene in DNA barcoding of amphibians. Frontiers in Zoology, 2, 5.

    Vences M., Thomas M., Bonett R.M. & Vieites D.R. (2005) Deciphering amphibian diversity through DNA barcoding: chances and challenges. Philosophical Transactions of the Royal Society of London. Series B, 360, 1859–1868.

    Wynn, A. & Heyer, W.R. (2001) Do geographically widespread species of tropical amphibians exist? An estimate of genetic relatedness within the neotropical frog Leptodactylus fuscus (Schneider 1799) (Anura Leptodactylidae). Tropical Zoology, 14, 255–285.

    Zimmerman, B.L. & Simberloff, D. (1996) An historical interpretation of habitat use by frogs in a Central Amazonian forest. Journal of Biogeography, 23, 27–46.