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Issue: Vol. 5315 No. 4: 11 Jul. 2023
Type: Article
Published: 2023-07-11
DOI: 10.11646/zootaxa.5315.4.3
Page range: 329-338
Abstract views: 194
PDF downloaded: 20

What do molecular data and vibrational communication tell us about the taxonomy of the genus Tyrrhenoleuctra Consiglio, 1957 (Plecoptera: Leuctridae) in Spain?

Eurofins Cavendish; S.L.U.; Ctra. Bailen-Motril; Parcela 102-B “Edificio de Cristal 2”; 18210; Peligros; Granada; Spain
Eurofins Cavendish; S.L.U.; Ctra. Bailen-Motril; Parcela 102-B “Edificio de Cristal 2”; 18210; Peligros; Granada; Spain
Departamento de Ecología; Facultad de Ciencias; Universidad de Granada; Campus Fuentenueva s/n; 18071; Granada; Spain
Dipartimento per la Innovazione nei Sistemi Biologici; Agroalimentari e Forestali; Università della Tuscia; 01100; Viterbo; Italy
Departamento de Zoología; Facultad de Ciencias; Universidad de Granada; Campus Fuentenueva s/n; 18071; Granada; Spain
Plecoptera Stoneflies Tyrrhenoleuctra drumming signals phylogenetic tree Tyrrhenoleuctra hynesi sp. n.

Abstract

In this paper we present the results of a study of different populations of the genus Tyrrhenoleuctra, which includes morphologically cryptic species, in the Iberian Peninsula. A total of six populations were analysed from a molecular point of view and a behavioural study was conducted on three of them. Two phylogenetic trees, maximum likelihood (ML) and neighbour joining (NJ), were constructed using both new COI sequences and COI sequences already available in GenBank. Furthermore, intra- and interspecific p-distances were calculated within and between sequences of the different populations. As regards behavioural analyses, the male call of individuals from three of the studied populations was recorded, analysed and described. Both phylogenetic approaches used, ML and NJ, were congruent in discriminating five well-defined clusters, representing the four known Iberian Tyrrhenoleuctra species and a previously unknown taxon. The sequences from the six studied populations produced in this study fell within two clades: T. lusohispanica (four populations) and the new taxon (two populations). These results were supported also by the behavioral study, in which the drumming calls of individuals belonging to the two clades differed in some important parameters such as the number of sequences, intersequence interval and interbeat interval. The obtained results expand the previously known distribution of T. lusohispanica and show the existence of a new species (Tyrrhenoleuctra hynesi sp. n.) within this genus from a more restricted area, that will be described in this paper.

 

References

  1. Aubert, J. (1963) Les Plécoptères de la Péninsule Iberique. Eos, 39, 23–107.
  2. Boumans, L. & Johnsen, A. (2015) Stonefly duets: vibrational sexual mimicry can explain complex patterns. Journal of Ethology, 33 (2), 87–107. https://doi.org/10.1007/s10164-015-0423-y DOI: https://doi.org/10.1007/s10164-015-0423-y
  3. Boumans, L. & Murányi, D. (2014) Two new species of Zwicknia Murányi, with molecular data on the phylogenetic position of the genus (Plecoptera, Capniidae). Zootaxa, 3808 (1), 001–091. https://doi.org/10.11646/zootaxa.3808.1.1 DOI: https://doi.org/10.11646/zootaxa.3808.1.1
  4. Burmeister, H. (1939) Plecoptera. In: Handbuch der Entomologie. Vol. 2. G. Reimer, Berlin, pp. 863–881.
  5. Consiglio, C. (1956) Note sul genere Strobliella Klapálek (Plecoptera). Memorie Società entomologica italiana, 35, 81–91.
  6. Consiglio, C. (1957) Tyrrhenoleuctra Nomen Novum. Bollettino della Società entomologica italiana, 87 (1/2), 17.
  7. Fernández-Calero, J.M., Bonada, N., Cañedo-Argüelles, M. & Tierno de Figueroa, J.M. (2022) Primera cita del género Tyrrhenoleuctra Consiglio, 1957 (Plecoptera, Leuctridae) en el noreste de la península ibérica (Cataluña, España). Boletín de la Asociación española de Entomología, 46 (1–2), 165–168.
  8. Ferreira, S., Tierno de Figueroa, J.M., Martins, F.M.S., Veríssimo, J., Quaglietta, L., Grosso-Silva, J.M., Lopes, P.B., Sousa, P., Paupério, J., Fonseca, N.A. & Beja, P. (2020) The InBIO Barcoding Initiative Database: contribution to the knowledge on DNA barcodes of Iberian Plecoptera. Biodiversity Data Journal, 8, e55137. https://doi.org/10.3897/BDJ.8.e55137 DOI: https://doi.org/10.3897/BDJ.8.e55137
  9. Fochetti, R., Ketmaier, V., Oliverio, M., Tierno de Figueroa, J.M. & Sezzi, E. (2004) Biochemical systematics and biogeography of the Mediterranean genus Tyrrhenoleuctra (Plecoptera, Insecta). Insect Systematics and Evolution, 35 (3), 299–306. https://doi.org/10.1163/187631204788920211 DOI: https://doi.org/10.1163/187631204788920211
  10. Fochetti, R., Sezzi, E., Tierno de Figueroa, J.M., Modica, M.V. & Oliverio, M. (2009) Molecular systematics and biogeography of the western Mediterranean stonefly genus Tyrrhenoleuctra (Plecoptera, Insecta). Journal of Zoological Systematics and Evolutionary Research, 47 (4), 328–336. https://doi.org/10.1111/j.1439-0469.2008.00508.x DOI: https://doi.org/10.1111/j.1439-0469.2008.00508.x
  11. Fochetti, R. & Tierno de Figueroa, J.M. (2009) A new species of Leuctridae discovered by means of molecular and biochemical approaches: Tyrrhenoleuctra antoninoi n. sp. (Insecta: Plecoptera). Zootaxa, 2112 (1), 41–46. https://doi.org/10.11646/zootaxa.2112.1.3 DOI: https://doi.org/10.11646/zootaxa.2112.1.3
  12. Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3 (5), 294–299.
  13. García de Jalón, D. & González del Tánago, M. (1986) Ephemeroptera. Plecoptera y Trichoptera de los principales ríos de Málaga. In: Actas del II Simposio sobre el agua en Andalucía, vol 2. Universidad de Granada, Granada, pp. 331–346
  14. González del Tánago, M. & García de Jalón, D. (1987) Clasificación de los ríos de Málaga según las comunidades de macrobentos. In: Toja, J. (Ed.), Actas del IV Congreso español de Limnología. Universidad de Sevilla/Asociación Española de Limnología, Sevilla, pp. 251–259. DOI: https://doi.org/10.23818/actas.04.26
  15. Guindon, S. & Gascuel, O. (2003) A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Systematic Biology, 52 (5), 696–704. https://doi.org/10.1080/10635150390235520 DOI: https://doi.org/10.1080/10635150390235520
  16. Kearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., Buxton, S., Cooper, A., Markowitz, S., Duran, C., Thierer, T., Ashton, B., Meintjes, P. & Drummond, A. (2012) Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics, 28 (12), 1647–1649. https://doi.org/10.1093/bioinformatics/bts199 DOI: https://doi.org/10.1093/bioinformatics/bts199
  17. Klapálek, F. (1905) Conspectus Plecopterorum Bohemiae. Časopisu České Společnosti Entomologické, 2, 27–32.
  18. López-Rodríguez, M.J., Luzón-Ortega, J.M. & Tierno de Figueroa, J.M. (2003) Contribución al conocimiento de los plecópteros (Insecta, Plecoptera) del Parque Natural de las Sierras de Almijara, Tejeda y Alhama y áreas próximas (SE España), con especial referencia a sus singularidades faunísticas. Boletín de la Sociedad Entomológica Aragonesa, 33, 167–171.
  19. López-Rodríguez, M.J. & Tierno de Figueroa, J.M. (2009) First record of the male drumming call in the genus Tyrrhenoleuctra Consiglio (Plecoptera: Leuctridae). Entomological News, 120 (2), 224–226. https://doi.org/10.3157/021.120.0216 DOI: https://doi.org/10.3157/021.120.0216
  20. López-Rodríguez, M.J., Tierno de Figueroa, J.M. & Alba-Tercedor, J. (2009) Life history, feeding and secondary production of two Nemouroidea species (Plecoptera, Insecta) in a temporary stream of the Southern Iberian Peninsula. Fundamental and applied limnology, 175 (2), 161–170. https://doi.org/10.1127/1863-9135/2009/0175-0161 DOI: https://doi.org/10.1127/1863-9135/2009/0175-0161
  21. Milne, I., Lindner, D., Bayer, M., Husmeier, D., McGuire, G., Marshal, D.F. & Wright, F. (2008) TOPA[1]Li v2: a rich graphical interface for evolutionary analyses of multiple alignments on HPC clusters and multi-core desktops. Bioinformatics, 25 (1), 126–127. https://doi.org/10.1093/bioinformatics/btn575 DOI: https://doi.org/10.1093/bioinformatics/btn575
  22. Murányi, D., Gamboa, M. & Orci, K.M. (2014) Zwicknia gen. n., a new genus for the Capnia bifrons species group. with descriptions of three new species based on morphology, drumming signals and molecular genetics, and a synopsis of the West Palaearctic and Nearctic genera of Capniidae (Plecoptera). Zootaxa, 3812 (1), 001–082. https://doi.org/10.11646/zootaxa.3812.1.1 DOI: https://doi.org/10.11646/zootaxa.3812.1.1
  23. Nelson, C.H. & Hanson, J.F. (1973) The genus Perlomyia (Plecoptera: Leuctridae). Journal of Kansas Entomological Society, 46 (2), 187–199.
  24. Pons, L. (1986) Presencia en Menorca del género Tyrrhenoleuctra (Insecta. Plecoptera). Miscellanea Zoologica, 10, 380–382.
  25. Puig, M.A., Ferreras-Romero, M. & García-Rojas, A. (1990) Morphological variability of Tyrrhenoleuctra minuta (Klapálek, 1903) in South-Spain. In: Campbell, I.C. (Ed.), Mayflies and Stoneflies: Life history and Biology. Kluwer Academic Publishers, Dordrecht, pp. 357–360. https://doi.org/10.1007/978-94-009-2397-3_44 DOI: https://doi.org/10.1007/978-94-009-2397-3_44
  26. Ratnasingham, S. & Hebert, P.D.N. (2007) BOLD: The Barcode of Life Data System (www.barcodinglife.org). Molecular Ecology Notes, 7, 355–364. https://doi.org/10.1111/j.1471-8286.2007.01678.x DOI: https://doi.org/10.1111/j.1471-8286.2007.01678.x
  27. Ris, F. (1905) Zwei notizen uber schweizerische Perliden. Mitteilungen der Schweizerischen Entomologischen Gesellschaft, 11 (2), 93–97.
  28. Ruffoni, A. & Tierno de Figueroa, J.M. (2022) Description of drumming signals of four stonefly species from Corsica (Plecoptera: Perlodidae, Nemouridae, Capniidae and Leuctridae). Aquatic Insects, 43 (2), 182–197. https://doi.org/10.1080/01650424.2021.1998541 DOI: https://doi.org/10.1080/01650424.2021.1998541
  29. Rupprecht, R. (1969) Zur Artspezifität der Trommelsignale der Plecopteren (Insecta). Oikos, 20 (1), 26–33. https://doi.org/10.2307/3543742 DOI: https://doi.org/10.2307/3543742
  30. Rupprecht, R. (1983) Kommunicationssignale von Arten der Gattung Isoperla (Plecoptera) und deren Einung zur Abgrenzung von Arten. Verhandlungen der deutschen Zoologischen Gesellschaft, 1983, 198.
  31. Sánchez-Ortega, A., Alba-Tercedor, J. & Tierno de Figueroa, J.M. (2002) Lista faunística y bibliográfica de los Plecópteros de la península ibérica e Islas Baleares. Publicaciones de la Asociación española de Limnología, Madrid, 198 pp.
  32. Sezzi, E. (2001) Biogeografia molecolare e storia evolutiva del genere Tyrrhenoleuctra (Plecoptera, Insecta) nelbacino del Mediterraneo. PhD Thesis, Universita`degli Studi della Tuscia, Viterbo, 93 pp.
  33. Shepard, W.D. & Baumann, R.W. (1995) Calileuctra. a new genus and two new species of stoneflies from California (Plecoptera: Leuctridae). Great Basin Naturalist, 55 (2), 124–134. https://doi.org/10.5962/bhl.part.22805 DOI: https://doi.org/10.5962/bhl.part.22805
  34. Stark, B.P. & Nelson, C.H. (2019) First SEM micrographs of representatives of Pachyleuctra Despax, 1929 and Tyrrhenoleuctra Consiglio, 1957 (Plecoptera: Leuctridae). Illiesia, 15 (06), 98–106. http://doi.org/10.5281/zenodo.4753114
  35. Stewart, K.W. (1997) Vibrational Communication in Insects. Epitome in the Language of Stoneflies? American Entomologist, 43 (2), 81–91. https://doi.org/10.1093/ae/43.2.81 DOI: https://doi.org/10.1093/ae/43.2.81
  36. Stewart, K.W. (2001) Vibrational communication (drumming) and mate-searching behavior of stoneflies (Plecoptera); evolutionary considerations. In: Dominguez, E. (Ed.), Trends in Research in Ephemeroptera and Plecoptera. Kluwer Academic/Plenum Publishers, New York, New York, pp. 217–225. https://doi.org/10.1007/978-1-4615-1257-8_25 DOI: https://doi.org/10.1007/978-1-4615-1257-8_25
  37. Stewart, K.W. & Sandberg, J.B. (2006) Vibrational communication and mate searching behaviour in stoneflies. In: Drosopoulos, S. & Claridge, M.F. (Eds.), Insect Sounds and Communication. Physiology, Behaviour, Ecology and Evolution. Taylor & Francis, Boca Raton, Florida, pp. 179–186. DOI: https://doi.org/10.1201/9781420039337.ch12
  38. Szczytko, S.W. & Stewart, K.W. (1979a) Drumming behavior of four Western Nearctic Isoperla (Plecoptera) species. Annals of the Entomological Society of America, 72 (6), 781–786. https://doi.org/10.1093/aesa/72.6.781 DOI: https://doi.org/10.1093/aesa/72.6.781
  39. Szczytko, S.W. & Stewart, K.W. (1979b) Stonefly drumming as a model classroom study of aquatic insect behavior. In: Resh, V.H. & Rosemberg, D.M. (Eds.), Innovative teaching in aquatic Entomology. Canadian Special Publication of Fisheries and Aquatic Sciences 43. Canadian Special Publication of Fisheries and Aquatic Sciences, Otawa, pp. 31–37.
  40. Tamura, K., Stecher, G. & Kumar, S. (2021) MEGA11: molecular evolutionary genetics analysis version 11. Molecular Biology and Evolution, 38 (7), 3022–3027. https://doi.org/10.1093/molbev/msab120 DOI: https://doi.org/10.1093/molbev/msab120
  41. Tierno de Figueroa, J.M. & Fochetti, R. (2014) A second new species of Tyrrhenoleuctra discovered by means of molecular data: Tyrrhenoleuctra lusohispanica n. sp. (Plecoptera: Leuctridae). Zootaxa, 3764 (5), 587–593. https://doi.org/10.11646/zootaxa.3764.5.7 DOI: https://doi.org/10.11646/zootaxa.3764.5.7
  42. Tierno de Figueroa, J.M., Gaetani, B., Luzón-Ortega, J.M., López-Rodríguez, M.J. & Fochetti, R. (2011a) On the identity of Isoperla curtata (Plecoptera: Perlodidae): behavioural and molecular approaches show the existence of two separate species. Zootaxa, 3000 (1), 49–58. https://doi.org/10.11646/zootaxa.3000.1.3 DOI: https://doi.org/10.11646/zootaxa.3000.1.3
  43. Tierno de Figueroa, J.M., Luzón-Ortega, J.M. & López-Rodríguez, M.J. (2011b) Intraspecific and interspecific variation in drumming signals in the genus Capnioneura (Plecoptera: Capniidae). Aquatic Insects, 33 (4), 335–341. https://doi.org/10.1080/01650424.2011.640334 DOI: https://doi.org/10.1080/01650424.2011.640334
  44. Tierno de Figueroa, J.M., Luzón‐Ortega, J.M. & López‐Rodríguez, M.J. (2014) First record of the drumming signals of stoneflies Capnopsis Morton, 1896 and Protonemura Kempny, 1898 genera (Plecoptera, Capniidae and Nemouridae). Entomological Science, 17 (3), 302–308. https://doi.org/10.1111/ens.12067 DOI: https://doi.org/10.1111/ens.12067
  45. Tierno de Figueroa, J.M., Luzón-Ortega, J.M. & Stewart, K.W. (2002) The drumming of Isoperla pallida Aubert and Guadalgenus franzi (Aubert) (Plecoptera, Perlodidae), and review and evolutionary considerations of Southern Iberian Peninsula Perlodidae. Zoological Science, 19 (8), 871–875. https://doi.org/10.2108/zsj.19.871 DOI: https://doi.org/10.2108/zsj.19.871
  46. Tierno de Figueroa, J.M. & Sánchez-Ortega, A. (1999) The male drumming call of Isoperla nevada Aubert, 1952 (Plecoptera, Perlodidae). Aquatic Insects, 21 (1), 33–38. https://doi.org/10.1076/aqin.21.1.33.4543 DOI: https://doi.org/10.1076/aqin.21.1.33.4543
  47. Zeigler, D.D. (1990) Observations pertinent to the role of sexual selection in the stonefly Pteronarcella badia (Plecoptera: Pteronarcyidae). Entomological News, 101 (5), 283–287.
  48. Zwick, P. (2004) Key to the West Palaearctic genera of stoneflies (Plecoptera) in the larval stage. Limnologica, 34 (4), 315–348. https://doi.org/10.1016/S0075-9511(04)80004-5 DOI: https://doi.org/10.1016/S0075-9511(04)80004-5