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Issue: Vol. 5315 No. 4: 11 Jul. 2023
Type: Article
Published: 2023-07-11
DOI: 10.11646/zootaxa.5315.4.3
Page range: 329-338
Abstract views: 198
PDF downloaded: 21

What do molecular data and vibrational communication tell us about the taxonomy of the genus Tyrrhenoleuctra Consiglio, 1957 (Plecoptera: Leuctridae) in Spain?

Eurofins Cavendish; S.L.U.; Ctra. Bailen-Motril; Parcela 102-B “Edificio de Cristal 2”; 18210; Peligros; Granada; Spain
Eurofins Cavendish; S.L.U.; Ctra. Bailen-Motril; Parcela 102-B “Edificio de Cristal 2”; 18210; Peligros; Granada; Spain
Departamento de Ecología; Facultad de Ciencias; Universidad de Granada; Campus Fuentenueva s/n; 18071; Granada; Spain
Dipartimento per la Innovazione nei Sistemi Biologici; Agroalimentari e Forestali; Università della Tuscia; 01100; Viterbo; Italy
Departamento de Zoología; Facultad de Ciencias; Universidad de Granada; Campus Fuentenueva s/n; 18071; Granada; Spain
Plecoptera Stoneflies Tyrrhenoleuctra drumming signals phylogenetic tree Tyrrhenoleuctra hynesi sp. n.

Abstract

In this paper we present the results of a study of different populations of the genus Tyrrhenoleuctra, which includes morphologically cryptic species, in the Iberian Peninsula. A total of six populations were analysed from a molecular point of view and a behavioural study was conducted on three of them. Two phylogenetic trees, maximum likelihood (ML) and neighbour joining (NJ), were constructed using both new COI sequences and COI sequences already available in GenBank. Furthermore, intra- and interspecific p-distances were calculated within and between sequences of the different populations. As regards behavioural analyses, the male call of individuals from three of the studied populations was recorded, analysed and described. Both phylogenetic approaches used, ML and NJ, were congruent in discriminating five well-defined clusters, representing the four known Iberian Tyrrhenoleuctra species and a previously unknown taxon. The sequences from the six studied populations produced in this study fell within two clades: T. lusohispanica (four populations) and the new taxon (two populations). These results were supported also by the behavioral study, in which the drumming calls of individuals belonging to the two clades differed in some important parameters such as the number of sequences, intersequence interval and interbeat interval. The obtained results expand the previously known distribution of T. lusohispanica and show the existence of a new species (Tyrrhenoleuctra hynesi sp. n.) within this genus from a more restricted area, that will be described in this paper.

 

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