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Issue: Vol. 5318 No. 4: 21 Jul. 2023
Type: Article
Published: 2023-07-21
DOI: 10.11646/zootaxa.5318.4.4
Page range: 504-514
Abstract views: 272
PDF downloaded: 16

Morphological and molecular characterization of Travassosinema viatorum n. sp. (Nematoda: Oxyuridomorpha: Travassosinematidae) from the invasive millipede Chamberlinius hualienensis Wang, 1956 (Diplopoda: Polydesmida: Paradoxosomatidae) in Okinawa, Japan

Instituto de Ecología y Sistemática; Carretera Varona 11835 e/ Oriente y Lindero; La Habana 19; CP 11900; Calabazar; Boyeros; La Habana; Cuba; Department of Environmental Biology; College of Bioscience & Biotechnology; Chubu University; 1200 Matsumoto; Kasugai; Aichi 487–8501; Japan
Instituto de Ecología y Sistemática; Carretera Varona 11835 e/ Oriente y Lindero; La Habana 19; CP 11900; Calabazar; Boyeros; La Habana; Cuba; Instituto de Ecología y Sistemática; Carretera Varona 11835 e/ Oriente y Lindero; La Habana 19; CP 11900; Calabazar; Boyeros; La Habana; Cuba
Department of Environmental Biology; College of Bioscience & Biotechnology; Chubu University; 1200 Matsumoto; Kasugai; Aichi 487–8501; Japan
Nematoda 18S rDNa 28S rDNa invasive species new species phylogeny SeM

Abstract

Travassosinema viatorum n. sp. (Nematoda: Oxyuridomorpha: Travassosinematidae) is described from the invasive millipede Chamberlinius hualienensis Wang, 1956 (Diplopoda: Polydesmida: Paradoxosomatidae) in Okinawa, Japan. Females of T. viatorum n. sp. resemble T. claudiae Morffe & Hasegawa, 2017; T. thyropygi Hunt, 1996 and T. travassosi Rao, 1958 by lacking both lateral alae and a contraction posterior to the vulva. The body length of T. viatorum n. sp. is shorter than the aforementioned species. It differs from T. claudiae and T. thyropygi by its comparatively longer oesophagus, more posterior vulva, lack of cuticular ribs in the alae of the umbraculum and in the position of the nerve ring and excretory pore. Travassosinema viatorum n. sp. can be differentiated from T. travassosi by the extension of the cephalic umbraculum and the smaller eggs. The phylogeny of T. viatorum n. sp. is inferred by the D2–D3 domains of the 28S rDNA. The new species is located in a monophyletic clade with other sequences of Travassosinema from millipedes. The comparison of the phylogenies of Travassosinema and their hosts points to host switching rather than coevolution as a mechanism to explain the host-nematode relationships. This constitutes the second species of the genus Travassosinema described from the Japanese archipelago and the first oxyuridomorph nematodes found in C. hualienensis.

 

References

  1. Adamson, M.L. (1987) Oxyuridan (Nematoda) parasites of Scaphiostreptus seychellarum with comments on the families Pulchrocephalidae Kloss, 1959 and Travassosinematidae Rao, 1958. Canadian Journal of Zoology, 65, 2747–2754. https://doi.org/10.1139/z87-416 DOI: https://doi.org/10.1139/z87-416
  2. Adamson, M.L. (1994) Evolutionary patters in life histories of Oxyurida. International Journal for Parasitology, 24 (8), 1167–1177. https://doi.org/10.1016/0020-7519(94)90189-9 DOI: https://doi.org/10.1016/0020-7519(94)90189-9
  3. Adamson, M.L. & Van Waerebeke, D. (1992) Revision of the Thelastomatoidea, Oxyurida of invertebrate hosts 2. Travassosinematidae, Protrelloididae and Pseudonymidae. Systematic Parasitology, 21, 169–188. https://doi.org/10.1007/BF00009698 DOI: https://doi.org/10.1007/BF00009698
  4. Blaxter, M.L., De Ley, P., Garey, J.R., Liu, L.X., Scheldeman, P., Vierstraete, A., Vanfleteren, J.R., Mackey, L.Y., Dorris, M., Frisse, L.M., Vida, J.T. & Thomas, W.K. (1998) A molecular evolutionary framework for the phylum Nematoda. Nature, 392, 71–75. https://doi.org/10.1038/32160 DOI: https://doi.org/10.1038/32160
  5. Capella-Gutiérrez, S., Silla-Martínez, J.M. & Gabaldón, T. (2009) trimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses. Bioinformatics, 25, 1972–1973. https://doi.org/10.1093/bioinformatics/btp348 DOI: https://doi.org/10.1093/bioinformatics/btp348
  6. Carta, L.K., Thomas, W.K. & Meyer-Rochow, V.B. (2018) Two nematodes (Nematoda: Diplogastridae, Rhabditidae) from the invasive millipede Chamberlinius hualienensis Wang, 1956 (Diplopoda, Paradoxosomatidae) on Hachijojima Island in Japan. Journal of Nematology, 4 (50), 479–486. https://doi.org/10.21307/jofnem-2018-048 DOI: https://doi.org/10.21307/jofnem-2018-048
  7. Dhivya, A. & Alagesan, P. (2017) Millipedes as Host for Microbes—A Review. International Journal of Microbiological Research, 8 (1), 19–24. https://doi.org/10.5829/idosi.ijmr.2017.19.24
  8. Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32, 1792–1797. https://doi.org/10.1093/nar/gkh340 DOI: https://doi.org/10.1093/nar/gkh340
  9. Falqueto, S.A., de Sousa, J.R., da Silva, R.C., da Silva, G.F., Pinheiro, D.G. & Soares, M.A. (2022) Larval gut microbiome of Pelidnota luridipes (Coleoptera: Scarabaeidae): high bacterial diversity, different metabolic profiles on gut chambers and species with probiotic potential. World Journal of Microbiology and Biotechnology, 38, 210. https://doi.org/10.1007/s11274-022-03387-1 DOI: https://doi.org/10.1007/s11274-022-03387-1
  10. Floyd, R.M., Rogers, A.D., Lambshead, P.J.D. & Smith, C.R. (2005) Nematode-specific PCR primers for the 18S small subunit rRNA gene. Molecular Ecology Notes, 5, 611–612. https://doi.org/10.1111/j.1471-8286.2005.01009.x DOI: https://doi.org/10.1111/j.1471-8286.2005.01009.x
  11. Giribet, G. & Edgecombe, G.D. (2019) The Phylogeny and Evolutionary History of Arthropods. Current Biology, 29, R592–R602. https://doi.org/10.1016/j.cub.2019.04.057 DOI: https://doi.org/10.1016/j.cub.2019.04.057
  12. Golovatch, S.E. & Kime, R.D. (2009) Millipede (Diplopoda) distributions: A review. Soil Organisms, 81 (3), 565–597. Available from: https://soil-organisms.org/index.php/SO/article/view/210 (accessed 7 March 2023)
  13. Guindon, S., Dufayard, J.-F., Lefort, V., Anisimova, M. Hordijk, W. & Gascuel, O. (2010) New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0. Systematic Biology, 59 (3), 307–321. https://doi.org/10.1093/sysbio/syq010 DOI: https://doi.org/10.1093/sysbio/syq010
  14. Hunt, D.J. (1981) On Artigasia horridospina n.sp., Longior semialata n.sp., Mentecle magnifica n.sp., Paraxyo ensicrinatus n.sp. (Oxyurida: Hystrignathidae) and Pulchrocephala? pulchrocephala Travassos, 1925 (Oxyurida: Pulchrocephalidae). Systematic Parasitology, 3, 33–52. https://doi.org/10.1007/BF00012238 DOI: https://doi.org/10.1007/BF00012238
  15. Hunt, D.J. (1993) Two new species of Travassosinema Rao, 1958 (Nematoda: Travassosinematidae) from diplopods in Sulawesi and Papua New Guinea. Afro-Asian Journal of Nematology, 3, 196–200.
  16. Hunt, D.J. (1996) Travassosinema thyropygi sp. n. (Nematoda: Travassosinematidae) from a spirobolid millipede from Vietnam with SEM observations on Heth imias Spiridonov, 1989 (Nematoda: Hethidae). Fundamental and Applied Nematology, 19, 7–14.
  17. Jex, A.R., Schneider, M.A., Rose, H.A. & Cribb, T.H. (2005) The Thelastomatoidea (Nematoda: Oxyurida) of two sympatric Panesthiinae (Insecta: Blattodea) from southeastern Queensland, Australia: taxonomy, species richness and host specificity. Nematology, 7, 543–575. https://doi.org/10.1163/156854105774384741 DOI: https://doi.org/10.1163/156854105774384741
  18. Minh, B.Q., Nguyen, M.A.T. & von Haeseler, A. (2013) Ultrafast Approximation for Phylogenetic Bootstrap. Molecular Biology and Evolution, 30 (5), 1188–1195. https://doi.org/10.1093/molbev/mst024 DOI: https://doi.org/10.1093/molbev/mst024
  19. Morffe, J., García, N., Adams, B.J. & Hasegawa, K. (2020) Three new species of Longior Travassos & Kloss, 1958 (Nematoda: Thelastomatoidea: Hystrignathidae) parasites of passalid beetles (Coleoptera: Passalidae) from Dominican Republic, Mexico and Colombia. Zootaxa, 4877 (1), 125–147. https://doi.org/10.11646/zootaxa.4877.1.5 DOI: https://doi.org/10.11646/zootaxa.4877.1.5
  20. Morffe, J., García, N., Véliz, L., Hasegawa, K. & Carreno, R.A. (2022) Morphological and molecular characterization of two species of nematodes (Oxyuridomorpha: Thelastomatoidea: Protrelloididae, Thelastomatidae) parasitic in the cockroach Blaberus discoidalis Serville (Blattaria: Blaberidae) from Cuba. Zootaxa, 5194 (1), 92–108. https://doi.org/10.11646/zootaxa.5194.1.5 DOI: https://doi.org/10.11646/zootaxa.5194.1.5
  21. Morffe, J. & Hasegawa, K. (2017) Morphological and molecular characterization of Travassosinema claudiae n. sp. (Oxyuridomorpha: Travassosinematidae) from the Japanese millipede Parafontaria laminata (Attems, 1909) (Polydesmida: Xystodesmidae). Zootaxa, 4282 (1), 166–178. https://doi.org/10.11646/zootaxa.4282.1.10 DOI: https://doi.org/10.11646/zootaxa.4282.1.10
  22. Nunn, G.B. (1992) Nematode molecular evolution. University of Nottingham, Nottingham, 187 pp.
  23. Rao, P.N. (1958) Studies on the nematode parasites of insects and other arthropods. Arquivos do Museo Nacional, Rio de Janeiro, 46, 33–84.
  24. Seinhorst, J.W. (1959) A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica, 4, 67–69. https://doi.org/10.1163/187529259X00381 DOI: https://doi.org/10.1163/187529259X00381
  25. Shah, N.A. & Shah, N. (2022) Ecological Benefits of Scarab beetles (Coleoptera: Scarabaeidae) on Nutrient Cycles: A Review Article. Advances in Biochemistry and Biotechnology, 7, 10109. https://doi.org/10.29011/2574-7258.010109 DOI: https://doi.org/10.29011/2574-7258.010109
  26. Soubrier, J., Steel, M., Lee, M.S., Der Sarkissian, C., Guindon, S., Ho, S.Y. & Cooper, A. (2012) The influence of rate heterogeneity among sites on the time dependence of molecular rates. Molecular Biology and Evolution Society, 29, 3345–3358. https://doi.org/10.1093/molbev/mss140 DOI: https://doi.org/10.1093/molbev/mss140
  27. Spiridonov, S.E. & Cribb, T.H. (2012) Travassosinema dalei sp. n. (Thelastomatoidea; Nematoda) from the hind gut of an Australian scarabaeid larva. New Zealand Journal of Zoology, 39, 291–300. https://doi.org/10.1080/03014223.2012.668855 DOI: https://doi.org/10.1080/03014223.2012.668855
  28. Spiridonov, S.E. & Ivanova, E.S. (1998) Parasitic nematodes of tropical megascolecid earthworm Pheretima leucocirca from Ba Vi National Park in Vietnam. Vestnik Zoologii, 32 (1–2), 40–50.
  29. Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729. https://doi.org/10.1093/molbev/mst197 DOI: https://doi.org/10.1093/molbev/mst197
  30. Trifinopoulos, J., Nguyen, L.T., von Haeseler, A. & Minh, B.Q. (2016) W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analysis. Nucleic Acids Research, 44 (W1), W232–W235. https://doi.org/10.1093/nar/gkw256 DOI: https://doi.org/10.1093/nar/gkw256