Abstract
Clistoabdominalis Skevington, 2001 has a cosmopolitan distribution and is a poorly studied genus of big-headed flies (Pipunculidae) in the Neotropical Region, completely unknown in Colombia. A new species is described from protected areas and conflict territories of limited access in Colombia, namely Clistoabdominalis lucyae sp. nov. (type-locality: Parque Nacional Natural Tayrona). Clistoabdominalis spinitibialis (Hardy, 1954) is recorded for the first time in Colombia, and an amended diagnosis provided. Illustrations and a dichotomic key to the Neotropical species of Clistoabdominalis are also presented.
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