Abstract
We compared the morphology of the internal and external reproductive organs of both sexes among species of the leaf beetle subfamily Donaciinae and its sister taxon Criocerinae. Using nine characters of the reproductive system, we attempted to reconstruct the phylogenetic tree for the two subfamilies combined. For this study, we used 11 Japanese species (including 2 subspecies) of 2 genera in the Donaciinae and 16 Japanese species of 4 genera in the Criocerinae. Morphological comparison revealed that the internal reproductive system exhibits a large diversity even in each subfamily. In particular, the morphology of the spermathecal organ varies greatly among genera or subgenera. In the subgenus Lema, we found a marked apomorphic state, that is, an extremely extended spermathecal duct in females and a similarly extended filiform structure on the internal sac in males. Phylogenetic analysis yielded two most parsimonious trees, which differ in criocerine relationship. In seven of the nine characters, the evolution of an apomorphic state was consistent with previous phylogenetic hypotheses.References
Askevold, I.S. (1987) The genus Neohaemonia Székessy in North America (Coleoptera: Chrysomelidae: Donaciinae): systematics, reconstructed phylogeny, and geographic history. Transactions of the American Entomological Society, 113, 360–430.
Askevold, I.S. (1990) Reconstructed phylogeny and reclassification of the genera of Donaciinae (Coleoptera: Chrysomelidae). Quaestiones Entomologicae, 26, 601–664.
Askevold, I.S. (1991) Classification, reconstructed phylogeny, and geographic history of the new world members of Plateumaris Thomson, 1859 (Coleoptera: Chrysomelidae: Donaciinae). Memoirs of the Entomological Society of Canada, 157, 1–175.
Chûjô, M. (1951) A taxonomic study on the Chrysomelidae (Insecta-Coleoptera) from Formosa (Part 1 – Subfamily Criocerinae). Technical Bulletin of the Kagawa-ken Agricultural College, 2, 71–120.
Duckett, C.N., Gillespie, J.J. & Kjer, K.M. (2004) Relationships among the subfamilies of Chrysomelidae inferred from small subunit ribosomal DNA and morphology, with special emphasis on the relationship among the flea beetles and the Galerucinae. In: Jolivet P. & Santiago-Blay J.A. and Schmitt M. (Eds.), New Developments in the Biology of Chrysomelidae. SPB Academic Publishing, The Netherlands, pp. 3–18.
Düngelhoef, S. & Schmitt, M. (2006) Functional morphology of copulation in Chrysomelidae-Criocerinae and Bruchidae (Insecta: Coleoptera). Bonner Zoologische Beiträge, 54, 201–208.
Farrell, B.D. (1998) “Inordinate fondness” explained: why are there so many beetles? Science, 281, 555–559.
Farrell, B.D. & Sequeira, A.S. (2004) Evolutionary rates in the adaptive radiation of beetles on plants. Evolution, 58, 1984–2001.
Gómez-Zurita, J., Hunt, T., Kopliku, F. & Vogler, A.P. (2007) Recalibrated tree of leaf beetles (Chrysomelidae) indicates independent diversification of angiosperms and their insect herbivores. PLoS ONE, 4, 1–8.
Gómez-Zurita, J., Hunt, T. & Vogler, A.P. (2008) Multilocus ribosomal RNA phylogeny of the leaf beetles (Chrysomelidae). Cladistics, 24, 34–50.
Hayashi, M. (2004) Revisional study on Japanese members of Donaciinae (Coleoptera: Chrysomelidae). Bulletin of the Hoshizaki Green Foundation, 7, 29–126 (In Japanese with English summary).
Hayashi, M. (2005) Morphological study on endophallus of Plateumarini and Donaciini (Coleoptera, Chrysomelidae, Donaciinae). Bulletin of the Hoshizaki Green Foundation, 8, 259–288. (In Japanese with English description and abstract.)
Hsiao, T.H. (1992) Molecular phylogeny of chrysomelid beetles inferred from mitochondrial DNA sequence data (Coleoptera: Chrysomelidae). In: Furth, D.G. (Ed), Proceedings of the Third International Symposium on the Chrysomelidae. Backhuys Publishers, The Netherlands, pp. 9–17.
Hsiao, T.H. (1994) Molecular techniques for studying systematics and phylogeny of Chrysomelidae. In: Jolivet, P.H., Cox, M.L. & Petitpierre, E. (Eds), Novel Aspects of the Biology of Chrysomelidae. Kluwer Academic Publishers, The Netherlands, pp. 237–248.
Kimoto, S. & Takizawa, H. (1994) Leaf Beetles (Chrysomelidae) of Japan. Tokai University, Tokyo, xvii+541 pp. (In Japanese with English keys)
Lee, J.E. (1993) Phylogenetic studies on the larvae of the Chrysomelidae (Coleoptera) from Japan. Japanese Journal of Entomology, 61, 409–424.
Lindroth, C.H. & Palmén, E. (1970; 2nd revised and enlarged edition) Coleoptera. In: Tuxen, S.L. (Ed), Taxonomist’s Glossary of Genitalia in Insects. Munksgaard, Copenhagen, 80–88.
Maddison, D.R. & Maddison, W.P. (2001) MacClade, Version 4.03. Sinauer, Sunderland, MA.
Mann, J.S. & Crowson, R.A. (1983a) On the internal male reproductive organs and their taxonomic significance in the leaf beetles (Coleoptera: Chrysomelidae). Entomologia Generalis, 9, 75–99.
Mann, J.S. & Crowson, R.A. (1983b) Observations on the internal anatomy and classification of Donaciinae (Coleoptera, Chrysomelidae). Entomologist’s Monthly Magazine, 119, 17–27.
Mann, S.J. & Crowson, R.A. (1996) Internal sac structure and phylogeny of Chrysomelidae. In: Jolivet, P.H.A. & Cox, M.L. (Eds), Chrysomelidae Biology. Vol. 1: The Classification, Phylogeny and Genetics. SPB Academic Publishing, Amsterdam, The Netherlands, pp. 291–316.
Mann, J.S. & Singh, J.P. (1979) Female reproductive system and genitalia of genus Lema (Criocerinae: Chrysomelidae: Coleoptera). Entomon 4, 89–94.
Monrós, F. (1959) Los generos de Chrysomelidae (Coleoptera). Opera Lilloana, Tucuman, 3, 1–337.
Pajni, H.R., Devi, N. & Tewari, P.K. (1985) A comparative account of the internal reproductive organs in family Chrysomelidae (Col.) III. Subfamilies Clytrinae, Cryptocephalinae and Criocerinae. Uttar Pradesh Journal of Zoology, 5, 130–138.
Powell, E.F. (1941) Relationships within the family Chrysomelidae (Coleoptera) as indicated by the male genitalia of certain species. The American Midland Naturalist, 25, 148–195.
Reid, C.A.M. (1995). A cladistic analysis of subfamilial relationships in the Chrysomelidae sensu lato (Chrysomeloidea). In: J. Pakaluk, and S. A. Slipinski. (Eds), Biology, Phylogeny and Classification of Coleoptera. Papers Celebrating the 80th Birthday of Roy A. Crowson. Vol. 2. Muzeum i Instytut Zoologii PAN, Warszawa, pp. 559–631.
Reid, C.A.M. (2000) Spilopyrinae Chapuis: a new subfamily in the Chrysomelidae and its systematic placement (Coleoptera). Invertebrate Taxonomy, 14, 837–862.
Rodríguez, V. (1994) Function of the spermathecal muscle in Chelymorpha alternans Boheman (Coleoptera: Chrysomelidae: Cassidinae). Physiological Entomology, 19, 198–202.
Rodríguez, V., Windsor, D.M. & Eberhard, W.G. (2004) Tortoise beetle genitalia and demonstration of a selected advantage for flagellum length in Cherymorpha alternans (Chrysomelidae, Cassidini, Stolaini). In: Jolivet, P., Santiago-Blay, J.A. & Schmitt, M. (Eds), New Developments in the Biology of Chrysomelidae. SPB Academic Publishing, Hague, The Netherlands, pp. 739–748.
Schmitt, M. (1985a) Versuch einer phylogenetisch-systematischen analyse der Criocerinae (Coleoptera, Chrysomelidae). Zoologische Beiträge, 29, 35–85.
Schmitt, M. (1985b) On the phylogeny of the Criocerinae (Coleoptera, Chrysomelidae). Entomography, 3, 393–401.
Schmitt, M. (1996) The phylogenetic system of the Chrysomelidae-history of ideas and present state of knowledge. In: Jolivet, P.H.A. & Cox, M.L. (Eds), Chrysomelidae Biology, Vol. 1: The Classification, Phylogeny and Genetics. SPB Academic Publishing, Amsterdam, The Netherlands, pp. 57–96.
Sharp, D. & Muir, F. (1912) The comparative anatomy of the male genitalia tube in Coleoptera. Transactions of the Entomological Society of London, 1912, 477–642.
Snodgrass, R.E. (1935) Principles of Insect Morphology. McGraw-Hill Book Co., New York and London, ix + 667 pp.
Sota, T. & Hayashi, M. (2004) A molecular phylogenetic analysis of the genus Donacia (Coleoptera, Chrysomelidae) in Japan, based on mitochondrial gene sequens. In: Jolivet, P., Santiago-Blay, J.A. & Schmitt, M. (Eds), New Developments in the Biology of Chrysomelidae. SPB Academic Publishing, Hague, The Netherlands, pp. 105–116.
Sota, T., Hayashi, M. & Yagi, T. (2007) Geographic variation in body and ovipositor size in the leaf beetle Plateumaris constricticollis (Coleoptera: Chrysomelidae) and its association with climatic conditions and host plants. European Journal of Entomology, 104, 165–172.
Suzuki, K. (1974) Ovariole number in the family Chrysomelidae (Insecta: Coleoptera). Journal of the College of Liberal Arts, Toyama University, 7, 53–70.
Suzuki, K. (1988) Comparative morphology of the internal reproductive system of Chrysomelidae (Coleoptera). In: Jolivet, P., Petitpierre, E. & Hsiao, T.H. (Eds), Biology of Chrysomelidae. Kluwer Academic Publishers, Dordrecht, Boston and London, pp. 317–355.
Suzuki, K. (1994) Observation method of the internal reproductive system in the Coleoptera using the dried and/or chemical-preserved specimens. Coleopterists’ News, 107, 7–10. (In Japanese.)
Suzuki, K. (1996) Higher classification on the family Chrysomelidae (Coleoptera). In: Jolivet, P.H.A. & Cox, M.L. (Eds), Chrysomelidae Biology, Vol. 1: The Classification, Phylogeny and Genetics. SPB Academic Publishing, Amsterdam, The Netherlands, pp. 3–54.
Suzuki, K. & Hara, A. (1975) Supplementary report on the ovariole number in the family Chrysomelidae (Insecta: Coleoptera). Journal of the College of Liberal Arts, Toyama University, 8, 87–93.
Suzuki, K. (2005) Description of a new species of the genus Lema (Coleoptera, Chrysomelidae, Criocerinae) from Honshu, Japan. Elytra, 33, 86–94.
Swofford, D.L. (2002) PAUP*. Phylogenetic analysis using parsimony (* and other methods). Version 4, Sinauer, Sunderland, MA.
Varma, B.K. (1955) Taxonomic value of spermathecal capsules as subfamily characters among the Chrysomelidae (Coleoptera). Indian Journal of Entomology, 17, 189–192.Vencl, F.V., Levy, A., Greeta, R., Keller, G. & Windsor, D.M. (2004) Observations on the natural history, systematics and phylogeny of the Criocerinae of Costa Rica and Panama. In: Jolivet, P., Santiago-Blay, J.A. & Schmitt, M. (Eds), New Developments in the Biology of Chrysomelidae. SPB Academic Publishing, Hague, The Netherlands, pp. 105–116.
Verma, K.K. (1985) Male reproductive organs as taxonomic characters for a broad classification of Chrysomelidae (Coleoptera). Entomography, 3, 485–487.
Verma, K.K. (1996) Inter-subfamily relations among Chrysomelidae (Coleoptera) as suggested by organization of the male genital system. In: Jolivet, P.H.A. & Cox, M.L. (Eds), Chrysomelidae Biology, Vol. 1: The Classification, Phylogeny and Genetics. SPB Academic Publishing, Amsterdam, The Netherlands, pp. 291–316.
Wanat, M. (2007) Alignment and homology of male terminalia in Curculionoidea and other Coleoptera. Invertebrate Systematics, 21, 147–171.
Wellso, S.G. (1972) Reproductive systems of the cereal leaf beetle. Comparison of morphology during seasonal development. Annals of the Entomological Society of America, 65, 945–949.