Skip to main content Skip to main navigation menu Skip to site footer
Type: Article
Published: 2023-11-15
Page range: 35-50
Abstract views: 333
PDF downloaded: 16

A new species of the Drosophila tripunctata group (Diptera: Drosophilidae) associated with fallen flowers of six Lecythidaceae species in the Amazon Rainforest

Programa de Pós-graduação em Biodiversidade e Evolução; Museu Paraense Emílio Goeldi (MPEG); Belém; PA; Brasil
Centro de Estudos Avançados da Biodiversidade; Universidade Federal do Pará (UFPA); Belém; PA; Brasil
Programa de Pós-graduação em Biodiversidade e Evolução; Museu Paraense Emílio Goeldi (MPEG); Belém; PA; Brasil
Centro de Ciências Biológicas; Universidade Federal do Pará (UFPA); Bragança; PA; Brasil
Museu Paraense Emílio Goeldi (MPEG); Belém; PA; Brasil
Diptera Brazil nut tree FLONA Caxiuanã emergence genetic insect Castanheira Caxiuanã emergência genética inseto

Abstract

The tripunctata group falls under the subgenus Drosophila Fallén, 1823 of the genus Drosophila Fallén, 1823, and is composed of four subgroups and 17 species not assigned to subgroups. This group is widely distributed throughout tropical regions and is predominantly found in preserved forest environments. With a predicted high number of cryptic species, the occurrence of intraspecific morphological polymorphisms made it difficult to establish lines in the laboratory. The capture of males for comparison of the terminalia makes it hard to identify and delimit species; however, these difficulties can be overcome through the use of techniques such as searching for species in naturally occurring places, establishing isofemale lines in the laboratory, or using molecular techniques. In this work, we search for imagoes of species of the tripunctata group over present fallen flowers of the Lecythidaceae on the ground of the Amazon rainforest. The collected individuals were morphologically and molecularly analyzed. This species is described here under the binomial Drosophila lecythus sp. nov.

 

References

  1. Amorim, B.S., Maciel, J.R., Melo, A. & Alves, M. (2016) Flora da Usina São José, Igarasu, Pernambuco: Lecythidaceae, Marcgraviaceae e Primulaceae. Rodriguésia, 7 (4), 971–979. https://doi.org/10.1590/2175-7860201667408
  2. Atkinson, W.D. & Shorrocks, R. (1977) Breeding site specificity in the domestic species of Drosophila. Oecologia, 29, 223–232. https://doi.org/10.1007/BF00345697
  3. Bächli, G. (2023) TaxoDros: the database on taxonomy of Drosophilidae. Available from: http://taxodros.unizh.ch/ (accessed 30 August 2023)
  4. Bächli, G., Vilela, C.R., Escher, S.A. & Saura, A. (2004) The Drosophilidae (Diptera) of Fennoscandia and Denmark. Fauna Entomologica Scandinavica, 39, 1–362. https://doi.org/10.1163/9789047414681
  5. Costa, A.C.L., Oliveira, A.A.R., Neto, G.C., Costa, M.C., Junior, J.A.S. & Portela, B.T.T. (2013) Caracterização meteorológica da Floresta Nacional de Caxiuanã. In: Lisboa, P.L.B. (Ed.), Caxiuanã: paraíso ainda preservado. Museu Paraense Emílio Goeldi, Belém, pp. 43–54.
  6. Cordeiro, J., Oliveira, J.H.F., Schmitz, H.J. & Vizentin-Bugoni, J. (2020) High niche partitioning promotes highly specialized, modular and non-nested florivore–plant networks across spatial scales and reveals drivers of specialization. Oikos, 129 (5), 619–629. https://doi.org/10.1111/oik.06866
  7. Dobzhansky, T. & Pavan, C. (1943) Studies on Brazilian species of Drosophila. In: Boletim da Faculdade de Filosofia, Ciências e Letras. Vol. 36. Universidade de São Paulo, São Paulo, pp. 7–72.
  8. Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32 (5),1792–1797. https://doi.org/10.1093/nar/gkh340
  9. Ferreira, L.V., Silva, A.S. & Almeida, S.S. (2012) Os tipos de vegetação de Caxiuanã in: Plano de Manejo da Floresta Nacional de Caxiuanã - Diagnóstico 1. Instituto Chico Mendes de Conservação da Biodiversidade, Brasília, Distrito Federal, pp. 132–152.
  10. Freire-Maia, N. & Pavan, C. (1949) Introdução ao estudo da Drosophila. Cultus, 5, 1–71.
  11. Frota-Pessoa, O. (1954) Revision of the tripunctata group of Drosophila with description of fifteen new species (Drosophilidae, Diptera). Arquivos do Museu Paranaense, 10, 253–304.
  12. Gottschalk, M.S., Bizzo, L., Döge, J.S., Profes, M.S., Hofmann, P.R.P. & Valente, V.L.S. (2009) Drosophilidae (Diptera) associated to fungi: differential use of resources in anthropic and Atlantic Rain Forest areas. Iheringia. Série Zoologia, 99 (4), 442–228. https://doi.org/10.1590/S0073-47212009000400016
  13. Hatadani, L.M., McInerney, J.O., Medeiros, H.F., Martins, A.C., Azeredo-Espin, A.M. & Klaczko, L.B. (2009) Molecular phylogeny of the Drosophila tripunctata and closely related species groups (Diptera: Drosophilidae). Molecular Phylogenetics and Evolution, 51 (3), 595–600. https://doi.org/10.1016/j.ympev.2009.02.022
  14. Kumar, S., Stecher, G., Li, M., Knyaz, C., Tamura, K. (2018) MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Molecular Biology and Evolution, 35 (6), 1547–1549. https://doi.org/10.1093/molbev/msy096
  15. Lorenzi, H. (1992) Árvores brasileiras: manual de identificação e cultivo de plantas arbóreas nativas do Brasil. Vol. 1. Editora Plantarum, Nova Odessa, São Paulo, 384 pp.
  16. Lorenzi H. (1998) Árvores brasileiras: manual de identificação e cultivo de plantas arbóreas nativas do Brasil. Vol. 2. Editora Plantarum, Nova Odessa, São Paulo, 352 pp.
  17. Martins, M.B. (1996) Drosófilas e outros insetos associados a frutos de Parahancornia amapa dispersos sobre o solo da floresta. Tese de Doutorado. Universidade Estadual de Campinas, Campinas, São Paulo, 203 pp.
  18. Martins, M.B. (2001) Guilds of Drosophilids on forest fragments. In: Bierregaard, R.O., Gascon, C., Lovejoy, T.E. & Mesquita, R. (Eds.), Lessons from Amazônia: The Ecology and Conservation of a fragmented forest. University Yale, Yale, New Haven, Connecticut, pp. 175–186.
  19. Martins, M.B. & Santos, R.C.O. (2005) Sítios de criação de Drosophila na Reserva Mocambo, Belém, Pará. In: Gomes, J.I., Martins, M.B., Silva, R.C.V. & Almeida, S.S. (Eds.), Mocambo: Diversidade e Dinâmica Biológica da Área de Pesquisa Ecológica do Guamá (APEG). Museu Paraense Emílio Goeldi; Embrapa Amazônia Oriental, Belém, Pará, pp. 389–404.
  20. Martins, M.B. & Oliveira, L. (2005) Dinâmica espacial de Drosophila em remanescente de mata na Reserva do Mocambo e suas áreas adjacentes. In: Gomes, J.I., Martins, M.B., Silva, R.C.V. & Almeida, S.S. (Eds.), Mocambo: Diversidade e Dinâmica Biológica da Área de Pesquisa Ecológica do Guamá (APEG). Museu Paraense Emílio Goeldi; Embrapa Amazônia Oriental, Belém, Pará, pp. 389–404.
  21. Mateus, R.P., Buschini, M.L.T. & Sene, F.M. (2006) The Drosophila community in xerophytic vegetations of the upper Parana-Paraguay river basin. Brazilian Journal of Biology, 66, 719–729. https://doi.org/10.1590/S1519-69842006000400016
  22. McEvey, S.F. & Grimaldi, D.A. (2021) Drosophilidae (Ferment Flies, Vinegar Flies or Fruit Flies). In: Kirk-Spriggs, A.H. & Sinclair, B.J. (Eds.), Manual of Afrotropical Diptera. Vol. 3. Brachycera - Cyclorrhapha, excluding Calyptratae. Suricata 8. South African National Biodiversity Institute, Pretoria, Gauteng, pp. 2295–2329.
  23. Morais, P.B., Martins, M.B., Klaczko, L.C., Mendonça-Hagler, L.C. & Hagler, A.N. (1995) Yeast succession in the Amazon fruit Parahancornia amapa as resource partitioning among Drosophila spp. Applied and Environmental Microbiology, 61, 4251–4257. https://doi.org/10.1128/aem.61.12.4251-4257.1995
  24. Mori, S.A. (1990) Diversificação e conservação das Lecythidaceae neotropicais. Acta Botanica Brasilica, 4 (1), 45–68. https://doi.org/10.1590/S0102-33061990000100004
  25. Mori, S.A., Kiernan, E.A., Smith, N.P., Kelley, L.M., Huang, Y.Y., Prance, G.T. & Thiers, B. (2016) Observations on the phytogeography of the Lecythidaceae clade (Brazil nut family). Phytoneuron, 2017 (30), 1–85.
  26. Mori, S.A., Smith, N.P. & Prance, G.T. (2010). As páginas Lecythidaceae. Jardim Botânico de Nova York, Bronx, Nova York. Available from: https://sweetgum.nybg.org/lp/index.php (accessed 30 August 2023)
  27. Pipkin, S.B. & Heed, W.B. (1964) Nine new members of the Drosophila tripunctata species group (Diptera: Drosophilidae). Pacific Insects, 6 (2), 256–273.
  28. Praxedes, C.L.B. & Martins, M.B., (2014) Inventário biológico: riqueza, biodiversidade local e regional de Drosophilidae (Diptera) frugívoros. In: Lisboa, P. (Ed.), Caxiuanã: Desafios para Conservação de uma Floresta Nacional na Amazônia. Vol. 4. Museu Paraense Emílio Goeldi, Belém, Pará, pp. 4 + 521–537.
  29. Procópio, L.C., Gayot, M., Sist, P. & Ferraz, I.D.K. (2010) As espécies de Tauari (Lecythidaceae) em florestas de terra firme da Amazônia: padrões de distribuição geográfica, abundâncias e implicações para a conservação. Acta Botanica Brasilica, 24 (4), 883–897. https://doi.org/10.1590/S0102-33062010000400002
  30. Procópio, L.C. & Secco, R.S. (2008) A importância da identificação botânica nos inventários florestais: o exemplo do “Tauari” (Couratari spp. e Cariniana spp.—Lecythidaceae) em duas áreas manejadas no Estado do Pará. Acta Amazonica, 38 (1), 31–44. https://doi.org/10.1590/S0044-59672008000100005
  31. QUIAGEN (2021) Protocolo para extração de DNA. Available from: https://www.qiagen.com/br/knowledge-and-support/product-and-technical-support/quality-and-safety-data/sds-search?l=BR&q=800000000562 (accessed 30 August 2023)
  32. Rambaut, A., Drummond, A.J., Xie, D., Baele, G. & Suchard, M.A. (2018) Posterior summarisation in Bayesian phylogenetics using Tracer 1.7. Systematic Biology, 67 (5), 901–904. https://doi.org/10.1093/sysbio/syy032
  33. Reis, C.M., Ruschel, A.R., Reis, L.P., Soares, M.H.M. & Cruz, E.D. (2016) Lecythidaceae encontradas na floresta de Moju, Pará. Embrapa Amazonia Oriental, Belém, Pará, 1 + 33 pp.
  34. Ribeiro, J.E.L.S., Hopkins, M.J.G., Vicentini, A., Sothers, C.A., Costa, M.A.S., Brito, J.M., Souza, M.A.D. Martins, L.H.P., Lohmann, L.G., Assunção, P.A.C.L., Pereira, E.C., Silva, C.F., Mesquita, M.R. & Procópio, L.C. (1999) Flora da Reserva Ducke. guia de identificação das plantas vasculares de uma floresta de terra-firme na Amazônia Central. Instituto Nacional de Pesquisas da Amazônia, Manaus, Amazonas, 793pp.
  35. Robe, L.J., Valente, V.L., Budnik, M. & Loreto, E.L. (2005) Molecular phylogeny of the subgenus Drosophila (Diptera, Drosophilidae) with an emphasis on neotropical species and groups: a nuclear versus mitochondrial gene approach. Molecular Phylogenetics and Evolution, 36, 623–640. https://doi.org/10.1016/j.ympev.2005.05.005
  36. Robe, L.J., Valente, V.L. & Loreto, E.L. (2010a) Phylogenetic relationships and macro-evolutionary patterns within the Drosophila tripunctata “radiation” (Diptera: Drosophilidae). Genetica, 138, 725–735. https://doi.org/10.1007/s10709-010-9453-0
  37. Robe, L.J., Loreto, E.L.S. & Valente, V.L.S. (2010b) Radiation of the ’’Drosophila‘‘ subgenus (Drosophilidae, Diptera) in the Neotropics. Journal of Zoological Systematics and Evolutionary Research, 48, 310–321. https://doi.org/10.1111/j.1439-0469.2009.00563.x
  38. Ronquist, F. & Huelsenbeck, J.P. (2003) MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574. https://doi.org/10.1093/bioinformatics/btg180
  39. Russo, C.A.M., Mello, B., Frazão, A. & Voloch, C.M. (2013) Phylogenetic analysis and a time tree for a large drosophilid data set (Diptera: Drosophilidae). Zoological Journal of the Linnean Society, 169, 765–775. https://doi.org/10.1111/zoj.12062
  40. Santa-Brígida, R., Schmitz, H.J. & Martins, M.B. (2017). Drosophilidae (Insecta, Diptera) in the state of Pará (Brazil). Biota Neotropica, 17 (1), e20160179. https://doi.org/10.1590/1676-0611-BN-2016-0179
  41. Schmitz, H.J., Hofmann, P.R.P. & Valente, V.L.S. (2010) Assemblages of drosophilids (Diptera, Drosophilidae) in mangrove forests: community ecology and species diversity. Iheringia, Série Zoologia, 100 (2), 133–140. https://doi.org/10.1590/S0073-47212010000200008
  42. Schmitz, H.J. & Valente, V.L.S. (2019) The flower flies and the unknown diversity of Drosophilidae (Diptera): a biodiversity inventory in the Brazilian fauna. Papéis Avulsos de Zoologia, 59, e20195945. https://doi.org/10.11606/1807-0205/2019.59.45
  43. Silva, A.A.R. & Martins, M.B. (2004) A new anthophilic species of Drosophila Fallén belonging to the bromeliae group of species (Diptera, Drosophilidae). Revista Brasileira de Zoologia, 21 (3), 435–437. https://doi.org/10.1590/S0101-81752004000300003
  44. Simon, C., Frati, F., Beckenbach, A., Crespi, B., Liu, H. & Flook, P. (1994). Evolution, weighting and phylogenetic utility of mithocondrial gene-sequences and compilation of conversed polymerase chain reaction primers. Annals of the Entomological Society of America, 87, 651–701. https://doi.org/10.1093/aesa/87.6.651
  45. Stamatakis, A. (2006) RaxML-VI-HPC: Maximum Likelihood-based Phylogenetic Analyses with Thousands of Taxa and Mixed Models. Bioinformatics, 22 (21), 2688–2690. https://doi.org/10.1093/bioinformatics/btl446
  46. Sturtevant, A. (1942) n.k. In: The classification of the genus Drosophila, with descriptions of nine new species. University of Texas Publications 4213. University of Texas, Austin, Texas, pp. 5–51.
  47. Throckmorton, L.H. (1975) The phylogeny, ecology and geography of Drosophila. In: King, R.C. (Ed.), Handbook of Genetics. Vol. 3. Plenum Publishing Corporation, New York, New York, pp. 421–469.
  48. Tidon, R. (2006) Relationships between drosophilids (Diptera, Drosophilidae) and the environment in two contrasting tropical vegetations. Biological Journal of the Linnean Society, 87, 233–247. https://doi.org/10.1111/j.1095-8312.2006.00570.x
  49. Val, F.C. (1982) The male genitalia of some neotropical Drosophila: notes and illustrations. Papéis Avulsos de Zoologia, 34 (27), 309–347.
  50. Vela, D. & Rafael, V. (2001) Ocho nuevas especies del grupo tripunctata, género Drosophila (Diptera, Drosophilidae), y el registro de Drosophila paraguayensis en el Bosque Protector Pasochoa, Pichincha Ecuador. Revista de la Pontificia Universidad Católica del Ecuador, 66, 92–120.
  51. Vela, D. & Rafael, V. (2005) Catorce nuevas especies del género Drosophila (Diptera, Drosophilidae) en el bosque húmedo montano del Volcán Pasochoa, Pichincha, Ecuador. Revista Ecuatoriana de Medicina y Ciencias Biológicas, 27, 27–41. https://doi.org/10.26807/remcb.v27i1-2.191
  52. Vilela, C. (1992) On the Drosophila tripunctata species group (Diptera, Drosophilidae). Revista Brasileira de Entomologia, 36, 197–221.
  53. Yassin, A. (2013) Phylogenetic classification of the Drosophilidae Rondani (Diptera): the role of morphology in the postgenomic era. Systematic Entomology, 38, 349–364. https://doi.org/10.1111/j.1365-3113.2012.00665.x
  54. Yokoto, K.S.C., Medeiros, H.F., Solferini, V.N. & Klaczko, L.B. (2003) A molecular study of the systematics of the Drosophila tripunctata group and the tripunctata radiation. Molecular Phylogenetics and Evolution, 28, 614–619. https://doi.org/10.1016/S1055-7903(03)00218-5