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Issue: Vol. 5471 No. 5: 21 Jun. 2024
Type: Article
Published: 2024-06-21
DOI: 10.11646/zootaxa.5471.5.4
Page range: 572-584
Abstract views: 56
PDF downloaded: 1

Hidden life cycle stages of the trypanosomatid Blastocrithidia triatomae Cerisola et al. 1971 (Protista: Excavata: Kinetoplastea) unveiled by a new cultivation method

Laboratório Interdisciplinar de Vigilância Entomológica de Diptera e Hemiptera; Instituto Oswaldo Cruz/FIOCRUZ; Rio de Janeiro/ RJ; Brazil; Laboratório de Insetos Vetores; Universidade de Vassouras; Campus Maricá; Maricá/RJ; Brazil; Faculdade de Ciências Médicas de Maricá; Maricá/RJ; Brazil
Laboratório Interdisciplinar de Vigilância Entomológica de Diptera e Hemiptera; Instituto Oswaldo Cruz/FIOCRUZ; Rio de Janeiro/ RJ; Brazil; Laboratório de Protistologia; Universidade Federal do Rio de Janeiro; Rio de Janeiro/RJ; Brazil
Laboratório Interdisciplinar de Vigilância Entomológica de Diptera e Hemiptera; Instituto Oswaldo Cruz/FIOCRUZ; Rio de Janeiro/ RJ; Brazil
Programa de Pós-graduação em Ciências Veterinárias da Universidade Federal Rural do Rio de Janeiro; Seropédica/RJ; Brazil
Programa de Iniciação Científica da Universidade Iguaçu; Universidade Iguaçu; Nova Iguaçu/RJ; Brazil
Laboratório de Diagnóstico Molecular e Hematologia; Universidade Federal do Rio de Janeiro; Rio de Janeiro; Brazil
Laboratório de Biodiversidade de Vetores e Patógenos; Universidade Federal Fluminense; Niterói/RJ; Brazil
Laboratório Interdisciplinar de Vigilância Entomológica de Diptera e Hemiptera; Instituto Oswaldo Cruz/FIOCRUZ; Rio de Janeiro/ RJ; Brazil; Programa de Iniciação Científica da Universidade Iguaçu; Universidade Iguaçu; Nova Iguaçu/RJ; Brazil; Escritório Regional FIOCRUZ Piauí; Teresina/PI; Brazil
Laboratório de Biologia de Insetos; Universidade Federal Fluminense; Niterói/RJ; Brazil
Protist Cultivation Morphology Morphometry Ontogeny Protista

Abstract

Blastocrithidia triatomae is a monoxenic trypanosomatid parasite of triatomines, sharing the same insect vectors with Trypanosoma cruzi Chagas, 1909 and T. rangeli Tejera, 1920. It is known to cause a complex syndrome in insects which induces severe metabolic disorders and increasing in mortality rates. In the present study, we established an efficient serial axenic cultivation method for B. triatomae using a new medium, herein called MBT, which revealed previously unknown morphotypes, named microepimastigotes and sinemastigotes, not found in specific cell linage cultures. The results improve the knowledge on the life cycle of genus Blastocrithidia Laird 1959, its distinction from other trypanosomatids, such as T. cruzi and T. rangeli, and settle new ground for thorough exploration of morphological features and its application for trypanosomatid systematics.

 

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