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Issue: Vol. 5514 No. 5: 1 Oct. 2024
Type: Article
Published: 2024-10-01
DOI: 10.11646/zootaxa.5514.5.3
Page range: 451-468
Abstract views: 392
PDF downloaded: 25

A new species of the genus Boulenophrys from South China (Anura, Megophryidae)

Guangdong Polytechnic of Environmental Protection Engineering; Foshan 528216; China
Guangdong Polytechnic of Environmental Protection Engineering; Foshan 528216; China
Guangdong Polytechnic of Environmental Protection Engineering; Foshan 528216; China
Guangdong Polytechnic of Environmental Protection Engineering; Foshan 528216; China
Guangdong Lianshan Bijiashan Provincial Nature Reserve; Qingyuan 513200; China
Guangzhou Shengheng Eco-forestry Company; Guangzhou 510663; China
Guangdong Wildlife Monitoring Rescue and Conservation Center; Guangzhou 510520; China
Guangdong Polytechnic of Environmental Protection Engineering; Foshan 528216; China,
Amphibia asian horned toads Boulenophrys pepe sp. nov. diversity winter

Abstract

In this study, a new species of the genus Boulenophrys is described from northwestern Guangdong, within the Nanling Moutains. The new species can be distinguished from all known congeners by a combination of morphological characteristics and the mitochondrial genetic divergence. The new species is found to breed in winter season which was not common in most amphibian groups but also present in some Boulenophrys congeners. We further discuss the inadequate surveys for these species due to the oversight of such multi-seasonal breeding pattern.

 

References

  1. Bonaparte, C.L.J.L. (1850) Conspectus Systematum Herpetologiae et Amphibiologiae. Editio altera reformata. [Lugduni Batavorum], Brill EJ., Leiden, 1 pp.
  2. Bourret, R. (1937) Notes herpétologiques sur l’Indochine française. XIV. Les batraciens de la collection du Laboratoire des Sciences Naturelles de l’Université. Descriptions de quinze especes ou variétés nouvelles. Annexe au Bulletin Général de l’Instruction Publique, 1937, 5–56.
  3. Dubois, A. (1980) Notes sur la systematique et la repartition des amphibiens anoures de Chine et des regions avoisinantes IV. Classification generique et subgenerique des Pelobatidae Megophryinae. Bulletin Mensuel de la Société Linnéenne de Lyon, 49 (8), 469–482. https://doi.org/10.3406/limly.1980.10444
  4. Dubois, A., Ohler, A. & Pyron, R.A. (2021) New concepts and methods for phylogenetic taxonomy and nomenclature in zoology, exemplified by a new ranked cladonomy of recent amphibians (Lissamphibia). Megataxa, 5 (1), 1–738. https://doi.org/10.11646/megataxa.5.1.1
  5. Fei, L., Hu, S.Q., Ye, C.Y. & Huang, Y.Z. (2009) Fauna Sinica. Amphibia. Vol. 2. Anura. Science Press, Beijing, 503 pp. [in Chinese]
  6. Fei, L. & Ye, C.Y. (2016) Amphibians of China. Vol. 1. Science Press, Beijing, 1040 pp.
  7. Fei, L., Ye, C.Y. & Huang Y.Z. (1983) Two new subspecies of Megophrys omeimontis Liu from China (Amphibia, Pelobatidae). Acta Herpetologica Sinica, 2 (2), 49–52. [in Chinese with English abstract]
  8. Frost, D.R. (2024) Amphibian Species of the World: an online reference. Version 6.2. American Museum of Natural History, New York, New York. Available from: https://amphibiansoftheworld.amnh.org/index.php. (accessed 8 September 2024)
  9. Hu, S.Q., Zhao, E.M. & Liu, C.C. (1973) A survey of amphibians and reptiles in Kweichow province, including a herpetofaunal analysis. Acta Zoologica Sinica/ Dong wu xue bao, 19, 149–181.
  10. Jiang, J.P., Ye, C.Y. & Fei, L. (2008) A new horn toad Megophrys sangzhiensis from Hunan, China (Amphibia, Anura). Zoological Research, 29 (2), 219–222. [in Chinese with English abstract] https://doi.org/10.3724/SP.J.1141.2008.00219
  11. Lanfear, R., Calcott, B., Ho, S.Y.W. & Guindon, S. (2012) PartitionFinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Molecular Biology & Evolution, 29 (6), 1695–1701. https://doi.org/10.1093/molbev/mss020
  12. Li, S.Z., Xu, N., Liu, J., Jiang, J.P., Wei, G. & Wang, B. (2018) A New Species of the Asian Toad Genus Megophrys sensu lato (Amphibia: Anura: Megophryidae) from Guizhou Province, China. Asian Herpetological Research, 9 (4), 224–239. https://doi.org/10.16373/j.cnki.ahr.180072
  13. Li, S.Z., Lu, N.N., Liu, J. & Wang, B. (2020) Description of a new Megophrys Kuhl & Van Hasselt, 1822 (Anura, Megophryidae) from Guizhou Province, China. ZooKeys, 986, 101–126. https://doi.org/10.3897/zookeys.986.57119
  14. Li, Y.L., Jin, M.J., Zhao, J., Liu, Z.Y., Wang, Y.Y. & Pang, H. (2014) Description of two new species of the genus Megophrys (Amphibia: Anura: Megophryidae) from Heishiding Nature Reserve, Fengkai, Guangdong, China, based on molecular and morphological data. Zootaxa, 3795 (4), 449–471. https://doi.org/10.11646/zootaxa.3795.4.5
  15. Liu, C.C. (1950) Amphibians of western China. Fieldiana:Zoology Memoires, 2, 1−397. https://doi.org/10.5962/bhl.part.4737
  16. Liu, J., Li, S., Wei, G., Xu, N., Cheng, Y., Wang, B. & Wu, J. (2020) A New Species of the Asian Toad Genus Megophrys sensu lato (Anura: Megophryidae) from Guizhou Province, China. Asian Herpetological Research, 11 (1), 1–18. https://doi.org/10.16373/j.cnki.ahr.190041
  17. Liu, Z.Y., Chen, G.L., Zhu, T.Q., Zeng, Z.C., Lyu, Z.T., Wang, J., Messenger, K., Greenberg, A.J., Guo, Z.X., Yang, Z.H., Shi, S.H. & Wang, Y.Y. (2018) Prevalence of cryptic species in morphologically uniform taxa – Fast speciation and evolutionary radiation in Asian frogs. Molecular Phylogenetics and Evolution, 127, 723–731. https://doi.org/10.1016/j.ympev.2018.06.020
  18. Luo, T., Wang, Y., Wang, S., Lu, X., Wang, W., Deng, H. & Zhou J. (2021) A species of the genus Panophrys (Anura, Megophryidae) from southeastern Guizhou Province, China. ZooKeys, 1047, 27–60. https://doi.org/10.3897/zookeys.1047.61097
  19. Lyu, Z.T., Li, Y.Q., Zeng, Z.C., Zhao, J., Liu, Z.L., Guo, G.X. & Wang, Y.Y. (2020) Four new species of Asian horned toads (Anura, Megophryidae, Megophrys) from southern China. ZooKeys, 942, 105–140. https://doi.org/10.3897/zookeys.942.47983
  20. Lyu, Z.T., Zeng, Z.C., Wang, J., Liu, Z.Y., Huang, Y.Q., Li, W.Z. & Wang, Y.Y. (2021) Four new species of Panophrys (Anura, Megophryidae) from eastern China, with discussion on the recognition of Panophrys as a distinct genus. Zootaxa, 4927 (1), 9–40. https://doi.org/10.11646/zootaxa.4927.1.2
  21. Lyu, Z.T., Qi, S., Wang, J., Zhang, S.Y., Zhao, J., Zeng, Z.C., Wan, H., Yang, J.H., Mo, Y.M. & Wang, Y.Y. (2023) Generic classification of Asian Horned Toads (Anura: Megophryidae: Megophryinae) and monograph of Chinese species. Zoological Research, 44 (2), 380–450. https://doi.org/10.24272/j.issn.2095-8137.2022.372
  22. Messenger, K.R., Dahn, H.A., Liang, Y., Xie, P., Wang, Y. & Lu, C. (2019) A new species of the genus Megophrys Gunther, 1864 (Amphibia: Anura: Megophryidae) from Mount Wuyi, China. Zootaxa, 4554 (2), 561–583. https://doi.org/10.11646/zootaxa.4554.2.9
  23. Mo, X.Y., Shen, Y.H., Li, H.H. & Wu, X.S. (2010) A new species of Megophrys (Amphibia: Anura: Megophryidae) from the northwestern Hunan Province, China. Current Zoology, 56 (4), 432–436. https://doi.org/10.1093/czoolo/56.4.432
  24. Nguyen, T.Q., Pham, C.T., Nguyen, T.T., Luong, A.M. & Ziegler, T. (2020) A new species of Megophrys (Amphibia: Anura: Megophryidae) from Vietnam. Zootaxa, 4722 (5), 401–422. https://doi.org/10.11646/zootaxa.4722.5.1
  25. Pope, C.H. (1929) Four new frogs from Fukien Province, China. American Museum Novitates, 352, 1–5.
  26. Qi, S., Lyu, Z.T., Wang, J., Mo, Y.M., Zeng, Z.C., Zeng, Y.J., Dai, K.Y., Li, Y.Q., Grismer, L.L. & Wang, Y.Y. (2021) Three new species of the genus Boulenophrys (Anura, Megophryidae) from southern China. Zootaxa, 5072 (5), 401–438. https://doi.org/10.11646/zootaxa.5072.5.1
  27. Qian, T.Y., Hu, K., Mo, X.Y., Gao, Z., Zhang, N. & Yang, D.D. (2023) A new species of Boulenophrys from central Hunan Province, China (Anura: Megophryidae). Vertebrate Zoology, 73, 915–930. https://doi.org/10.3897/vz.73.e100889
  28. Rao, D.Q. & Yang, D.T. (1997) The karyotypes of Megophryinae (Pelobatinae) with a discussion on their classification and phylogenetic relationships. Asiatic Herpetological Research, 7, 93–102. https://doi.org/10.5962/bhl.part.18858
  29. Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19 (12), 1572–1574. https://doi.org/10.1093/bioinformatics/btg180
  30. Shi, S.C., Li, D.H., Zhu, W.B., Jiang, W., Jiang, J.P. & Wang, B. (2021) Description of a new toad of Megophrys Kuhl & Van Hasselt, 1822 (Amphibia: Anura: Megophryidae) from western Yunnan Province, China. Zootaxa, 4942 (3), 351–381. https://doi.org/10.11646/zootaxa.4942.3.3
  31. Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics, 30 (9), 1312–1313. https://doi.org/10.1093/bioinformatics/btu033
  32. Silvestro, D. & Michalak, I. (2012) raxmlGUI: A graphical front-end for RAxML. Organisms, Diversity & Evolution, 12, 335–337. https://doi.org/10.1007/s13127-011-0056-0
  33. Su, H.J., Shi, S.C., Wu, Y.Q., Li, G.R., Yao, X.G., Wang, B. & Li, S.Z. (2020) Description of a new horned toad of Megophrys Kuhl & Van Hasselt, 1822 (Anura, Megophryidae) from southwest China. Zookeys, 974, 131–159. https://doi.org/10.3897/zookeys.974.56070
  34. Shang, Y., Luo, Y., Qian, T., Gao, Z. Li, Y., Deng, G. & Yang, D. (2023) Taxonomic discussion of Boulenophrys in Mangshan Mountain, Hunan, China and three new provincial records. Life Science Research, 27 (4), 349–360. [in Chinese with English abstract] https://doi.org/10.16605/j.cnki.1007-7847.2022.12.0235
  35. Shen, Y.H. (1994) A new pelobatid toad of the genus Megophrys from China (Anura: Pelobatidae). In: Collection of Articles for the 60th Anniversary of the Foundation of the Zoological Society of China. Zoological Society, Nanking, pp. 603–606. [in Chinese]
  36. Tapley, B., Cutajar, T., Mahony, S., Nguyen, C.T., Dau, V.Q., Nguyen, T.T., Luong, H.V. & Rowley, J.J.L. (2017) The Vietnamese population of Megophrys kuatunensis (Amphibia: Megophryidae) represents a new species of Asian horned frog from Vietnam and southern China. Zootaxa, 4344 (3), 465–492. https://doi.org/10.11646/zootaxa.4344.3.3
  37. Tapley, B., Cutajar, T.P., Mahony, S., Nguyen, C.T., Dau, V.Q., Luong, A.M., Le, D.T., Nguyen, T.T., Nguyen, T.Q., Portway, C., Luong, H.V. & Rowley, J.J.L. (2018a) Two new and potentially highly threatened Megophrys Horned frogs (Amphibia: Megophryidae) from Indochina’s highest mountains. Zootaxa, 4508 (3), 301–333. https://doi.org/10.11646/zootaxa.4508.3.1
  38. Tapley, B., Cutajar, T., Nguyen, L.T., Nguyen, C.T., Harding, L., Portway, C., Van Luong, H. & Rowley, J.J. (2018b) A new locality and elevation extension for Megophrys rubrimera (Tapley et al., 2017) in Bat Xat Nature Reserve, Lao Cai Province, northern Vietnam. Herpetology Notes, 11, 865–868.
  39. Tapley, B., Cutajar, T., Nguyen, L.T., Portway, C., Mahony, S., Nguyen, C.T., Harding, L., Luong, H.V. & Rowley, J.J.L. (2021) A new potentially Endangered species of Megophrys (Amphibia: Megophryidae) from Mount Ky Quan San, north-west Vietnam. Journal of Natural History, 54, 2543–2575. https://doi.org/10.1080/00222933.2020.1856952
  40. Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25 (24), 4876–4882. https://doi.org/10.1093/nar/25.24.4876
  41. Tian, Y.Z. & Sun, A. (1995) A new species of Megophrys from China (Amphibia: Pelobatidae). Journal of Liupanshui Teachers College, 52 (3), 11–15. [in Chinese]
  42. Tian, Y.Z., Gu, X.M. & Sun, A.Q. (2000) A new species of Megophrys in China (Amphibia: Pelobatidae). Acta Zootaxonomica Sinica, 25 (4), 462–466. [in Chinese]
  43. Wang, B., Wu, Y.Q., Peng, J.W., Shi, S.C., Lu, N.N. & Wu, J. (2020) A new Megophrys Kuhl & Van Hasselt (Amphibia, Megophryidae) from southeastern China. ZooKeys, 904, 35–62. https://doi.org/10.3897/zookeys.904.47354
  44. Wang, J., Liu, Z.Y., Lyu, Z.T., Zeng, Z.C. & Wang, Y.Y. (2017a) A new species of the genus Xenophrys (Amphibia: Anura: Megophryidae) from an offshore island in Guangdong Province, southeastern China. Zootaxa, 4324 (3), 541–556. https://doi.org/10.11646/zootaxa.4324.3.8
  45. Wang, J., Lyu, Z.T., Liu, Z.Y., Liao, C.K., Zeng, Z.C., Zhao, J., Li, Y.L. & Wang, Y.Y. (2019a) Description of six new species of the subgenus Panophrys within the genus Megophrys (Anura, Megophryidae) from southeastern China based on molecular and morphological data. ZooKeys, 851, 113–164. https://doi.org/10.3897/zookeys.851.29107
  46. Wang, J., Zeng, Z.C., Lyu, Z.T., Qi, S., Liu, Z.Y., Chen, H.H., Lu, Y.H., Xiao, H.W., Lin, C.R., Chen, K. & Wang, Y.Y. (2022) Description of three new Boulenophrys species from eastern Guangdong, China, emphasizing the urgency of ecological conservation in this region (Anura, Megophryidae). Zootaxa, 5099 (1), 91–119. https://doi.org/10.11646/zootaxa.5099.1.4
  47. Wang, L., Deng, X., Liu, Y., Wu, Q. & Liu, Z. (2019b) A new species of the genus Megophrys (Amphibia: Anura: Megophryidae) from Hunan, China. Zootaxa, 4695 (4), 301–330. https://doi.org/10.11646/zootaxa.4695.4.1
  48. Wang, Y.F., Liu, B.Q., Jiang, K., Jin, W., Xu, J.N. & Wu, C.H. (2017b) A new species of the Horn Toad of the genus Xenophrys from Zhejiang, China (Amphibia: Megophryidae). Chinese Journal of Zoology, 52 (1), 19–29. [in Chinese with English abstract] https://doi.org/10.13859/j.cjz.201701003
  49. Wang, Y.Y., Zhang, T.D., Zhao, J., Sung, Y.H., Yang, J.H., Pang, H. & Zhang, Z. (2012) Description of a new species of the genus Xenophrys Günther, 1864 (Amphibia: Anura: Megophryidae) from Mount Jinggang, China, based on molecular and morphological data. Zootaxa, 3546 (1), 53–67. https://doi.org/10.11646/zootaxa.3546.1.4
  50. Wang, Y.Y., Zhao, J., Yang, J.H., Zhou, Z., Chen, G.L. & Liu, Y. (2014) Morphology, molecular genetics, and bioacoustics support two new sympatric Xenophrys toads (Amphibia: Anura: Megophryidae) in Southeast China. PLoS ONE, 9 (4), e93075. https://doi.org/10.1371/journal.pone.0093075
  51. Wu, Y.H., Suwannapoom, C., Poyarkov, N.A., Chen, J.M., Pawangkhanant, P., Xu, K., Jin, J.Q., Murphy, R.W. & Che, J. (2019) A new species of the genus Xenophrys (Anura: Megophryidae) from northern Thailand. Zoological Research, 40 (6), 564–574. https://doi.org/10.24272/j.issn.2095-8137.2019.032
  52. Wu, Y.Q., Li, S.Z., Liu, W., Wang, B. & Wu, J. (2020) Description of a new horned toad of Megophrys Kuhl & Van Hasselt, 1822 (Amphibia, Megophryidae) from Zhejiang Province, China. ZooKeys, 1005, 73–102. https://doi.org/10.3897/zookeys.1005.58629
  53. Xu, N., Li, S.Z., Liu, J., Wei, G. & Wang, B. (2020) A new species of the horned toad Megophrys Kuhl & Van Hasselt, 1822 (Anura, Megophryidae) from southwest China. ZooKeys, 943, 119–144. https://doi.org/10.3897/zookeys.943.50343
  54. Ye, C.Y. & Fei, L. (1995) Taxonomic studies on the small type Megophrys in China including descriptions of the new species (subspecies) (Peloatidae: genus Megophrys). Acta Herpetologica Sinica, 4–5, 72–81. [in Chinese]
  55. Ye, C.Y., Fei, L. & Xie, F. (2007) A new species of Megophryidae – Megophrys baolongensis from China (Amphibia, Anura). Herpetologica Sinica, 11, 38–41. [in Chinese]
  56. Zeng, Z.C., Wang, J., Chen, H.H., Xiao, W.W., Zhan, B.B., Li, Y.H. & Lin, S.S. (2024) A new species of the genus Boulenophrys (Anura, Megophryidae) from Eastern Guangdong, China. Asian Herpetological Research, 15 (1), 12–21. https://doi.org/10.3724/ahr.2095-0357.2023.0038
  57. Zhang, Y., Li, G., Xiao, N., Li, J., Pan, T., Wang, H., Zhang, B. & Zhou, J. (2017) A new species of the genus Xenophrys (Amphibia: Anura: Megophryidae) from Libo County, Guizhou, China. Asian Herpetological Research, 8 (2), 75–85. https://doi.org/10.16373/j.cnki.ahr.160041