Skip to main content Skip to main navigation menu Skip to site footer
Responsive image
Issue: Vol. 5723 No. 1: 24 Nov. 2025
Type: Article
Published: 2025-11-24
DOI: 10.11646/zootaxa.5723.1.4
Page range: 93-104
Abstract views: 31
PDF downloaded: 3

Morphological redescription and DNA barcoding of Diamesa longipes Goetghebuer, 1941 (Diptera: Chironomidae: Diamesinae) from the Swiss Alps

Department of Zoology; Cantonal Museum of Natural Sciences; Palais de Rumine; Place de la Riponne 6; 1005 Lausanne; Switzerland.
Federal Scientific Center of the East Asia Terrestrial Biodiversity; Far East Branch of the Russian Academy of Sciences; 100 let Vladivostoku 159; 690022 Vladivostok; Russia.
Federal Scientific Center of the East Asia Terrestrial Biodiversity; Far East Branch of the Russian Academy of Sciences; 100 let Vladivostoku 159; 690022 Vladivostok; Russia.
Diptera Chironomidae Diamesinae Diamesa longipes syntype redescription morphology DNA barcoding Alpine mountains Switzerland

Abstract

Morphological redescription of Diamesa longipes Goetghebuer with using syntype from the Ötztal Alps (Tyrol) as well as the adult male and pupa from the Zermatt valley (canton Valais) downstream of the Gornera glacier (Swiss Alps) is provided, and DNA barcoding for specimens from Swiss Alps is studied. Based on the morphology and DNA barcoding using 658-bp fragments of the mitochondrial cytochrome oxidase subunit I, indicate that D. longipes is closely related to Diamesa zagrosica Makarchenko et Semenchenko from Iran. Mean K2P interspecific distances between D. longipes and D. zagrosica were 4.72% which based on four species’ delimitation approaches (BIN BOLD, ASAP, mPTP, GMYC), confirms that these are valid species. Comments on the geographical distribution and ecology of this rare species in the glacial streams of the Swiss Alps are also given.

 

References

  1. Alther, R., Thompson, C., Lods‑Crozet, B. & Robinson, C.T. (2019) Macroinvertebrate diversity and rarity in non‑glacial alpine streams. Aquatic Science, 81, 42. https://doi.org/10.1007/s00027-019-0642-3
  2. Ashe, P. & O'Connor, J.P. (2009) A World Catalogue of Chironomidae (Diptera). Part 1. Buchonomyiinae, Chilenomyiinae, Podonominae, Aphroteniinae, Tanypodinae, Usambaromyiinae, Diamesinae, Prodiamesinae and Telmatogetoninae. Irish Biogeographical Society & National Museum of Ireland, Dublin, 445 pp.
  3. Drummond, A.J., Suchard, M.A., Xie, D. & Rambaut, A. (2012) Bayesian Phylogenetics with BEAUti and the BEAST 1.7. Molecular Biology and Evolution, 29 (8), 1969–1973. https://doi.org/10.1093/molbev/mss075
  4. Felsenstein, J. (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. Journal of Molecular Evolution, 17, 368–376. https://doi.org/10.1007/BF01734359
  5. Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294–299.
  6. Fujisawa, T. & Barraclough, T.G. (2013) Delimiting species using single‑locus data and the Generalized Mixed Yule Coalescent approach: A revised method and evaluation on simulated data sets. Systematic Biology, 62 (5), 707–724. https://doi.org/10.1093/sysbio/syt033
  7. Gernhard, T. (2008) The conditioned reconstructed process. The Journal of Theoretical Biology, 253 (4), 769–778. https://doi.org/10.1016/j.jtbi.2008.04.005
  8. Goetghebuer, M. (1941) Quelques Chironomides du Tyrol. Musée Royal d’Histoire naturelle de Belgique, 17 (37), 1–8.
  9. Hasegawa, M., Kishino, H. & Yano, T. (1985) Dating of the human–ape splitting by a molecular clock of mitochondrial DNA. Journal of Molecular Evolution, 22, 160–174. https://doi.org/10.1007/bf02101694
  10. Kapli, P., Lutteropp, S., Zhang, J., Kobert, K., Pavlidis, P., Stamatakis, A & Flouri, T. (2017) Multi‑rate Poisson tree processes for singlelocus species delimitation under maximum likelihood and Markov chain Monte Carlo. Bioinformatics, 33 (11), 1630–1638. https://doi.org/10.1093/bioinformatics/btx025
  11. Kimura, M. (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16 (2), 111–120. https://doi.org/10.1007/BF01731581
  12. Kownacka, M. & Kownacki, A. (1975) Gletscherbach‑Zuckmucken der Oetztaler Alpen in Tirol (Diptera: Chironomidae: Diamesinae). Entomologica Germanica, 2 (1), 35–43. https://doi.org/10.1127/entom.germ/2/1975/35
  13. Kownacki, A. & Kownacka, M. (1973) Chironomidae (Diptera) from the Caucasus, Diamesa Waltl group steinboecki. Bulletin de l’Académie Polonaise des Sciences, Cl. II, Serie des sciences biologiques, 21, 27–37.
  14. Kownacki, A. (1978) Ecology and biogeography of the Diamesa steinboecki group. Acta Universitatis Carolinae – Biologica, 95–102.
  15. Lanfear, R., Calcott, B., Ho, S.Y & Guindon, S. (2012) Partitionfinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Molecular Biology and Evolution, 29 (6), 1695–1701. https://doi.org/10.1093/molbev/mss020
  16. Lencioni, V. & Rossaro, B. (2005) Microdistribution of chironomids (Diptera: Chironomidae) in Alpine streams: An autoecological perspective. Hydrobiologia, 533, 61–76. https://doi.org/10.1007/s10750-004-2393-x
  17. Lencioni, V., Rodriguez–Prieto, A. & Allegrucci, G. (2021) Congruence between molecular and morphological systematics of Alpine non–biting midges (Chironomidae, Diamesinae). Zoologica Scripta, 50 (4), 455–472. https://doi.org/10.1111/zsc.12480
  18. Lods‑Crozet, B. (2024) Chironomidae (last update: Jul 2024). In: Haenni, J.-P., Bächli, G., Bernasconi, M., Dufour, C., Fisler, L., Gonseth, Y., Lods‑Crozet, B., Monnerat, C. & Pollini Paltrinieri, L. (Eds.), Diptera – Checklist. Fauna Helvetica 35. Info fauna & SEG, Neuchâtel, pp. 102–120.
  19. Lods‑Crozet, B., Castella, E., Cambin, D., Ilg, C., Knispel, S. & Mayor‑Simeant, H. (2001) Macroinvertebrate community structure in relation to environmental variables in a Swiss glacial stream. Freshwater Biology, 46, 1641–1661. https://doi.org/10.1046/j.1365-2427.2001.00850.x
  20. Makarchenko, E.A., Semenchenko, A.A. & Palatov, D.M. (2022) Taxonomy of Diamesa steinboecki group (Diptera: Chironomidae: Diamesinae), with description and DNA barcoding of new species. I. Subgroups steinboecki and longipes. Zootaxa, 5125 (5), 483–512. https://doi.org/10.11646/zootaxa.5125.5.2
  21. Makarchenko, E.A., Semenchenko, A.A. & Palatov, D.M. (2023) Fauna and taxonomy of Diamesinae (Diptera, Chironomidae) from the Caucasus, with a morphological description and DNA barcoding of new taxa and a discussion of diagnostic problems for Diamesa Meigen and Pseudodiamesa Goetghebuer. Zootaxa, 5271 (2), 313–328. https://doi.org/10.11646/zootaxa.5271.2.6
  22. Moller Pillot, H.K.M. (2014) Chironomidae Larvae. Vol. 3. Orthocladiinae: Biology and Ecology of the Aquatic Orthocladiinae. KNNV Publishing, Zeist, 314 pp. https://doi.org/10.1163/9789004278059
  23. Montagna, M., Urbanelli, S. & Rossaro, B. (2016) The species of the genus Diamesa (Diptera, Chironomidae) known to occur in Italian Alps and Apennines. Zootaxa, 4193 (2), 317–331. https://doi.org/10.11646/zootaxa.4193.2.7
  24. Namayandeh, A., Ghaderi, E., Mohammadi, H. & Ghobari, H. (2021) An overview of the Iranian Chironomidae (Diptera) diversity with an updated checklist with the provincial distribution. Caucasian Entomological Bulletin, 17 (2), 401–407. https://doi.org/10.23885/181433262021172-401407
  25. Pankratova, V.Ya. (1970) Larvae and pupae of the midges of the subfamily Orthocladiinae (Diptera, Chironomidae = Tendipedidae) of the USSR fauna. In: Key to the USSR fauna, published by Zoological Institute of the USSR Academy of Sciences. Vol. 102. Nauka, Leningrad, pp. 1–344. [in Russian]
  26. Puillandre, N., Brouillet, S. & Achaz, G. (2021) ASAP: assemble species by automatic partitioning. Molecular Ecology Resources, 21 (2), 609–620. https://doi.org/10.1111/1755-0998.13281
  27. Rambaut, A., Drummond, A.J., Xie, D., Baele, G. & Suchard, M.A. (2018) Posterior summarisation in Bayesian phylogenetics using Tracer 1.7. Systematic Biology, 67 (5), 901–904. https://doi.org/10.1093/sysbio/syy032
  28. Ratnasingham, S. & Hebert, P.D. (2013) A DNA‑based registry for all animal species: the barcode index number (BIN) system. PLoS One, 8 (7), e66213. https://doi.org/10.1371/journal.pone.0066213
  29. Robinson, C.T., Jolidon, C. & Lods‑Crozet, B. (2024) Long‑term Patterns of Stream Macroinvertebrates in an Alpine Cirque Landscape. Aquatic Science, 86, 91. https://doi.org/10.1007/s00027-024-01106-6
  30. Ronquist, F., Teslenko, M., Mark, P.V.D., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L. & Huelsenbeck, J.P. (2012) MrBayes 3.2: Efficient Bayesian Phylogenetic Inference and Model Choice Across a Large Model Space. Systematic Biology, 61, 539–542. https://doi.org/10.1093/sysbio/sys029
  31. Rossaro, B., Pirola, N., Marziali, L., Magoga, G., Boggero, A. & Montagna, M. (2019) An updated list of chironomid species from Italy with biogeographic considerations (Diptera, Chironomidae). Biogeographia – The Journal of Integrative Biogeography, 34, 59–85. https://doi.org/10.21426/B634043047
  32. Sæther, O.A. (1980) Glossary of chironomid morphology terminology (Diptera, Chironomidae). Entomologica scandinavica, Supplement 14, 1–51.
  33. Semenchenko, A.A. & Makarchenko, E.A. (2025) On the taxonomy and distribution of Diamesa gregsoni Edwards, (Diptera: Chironomidae: Diamesinae), with morphological redescription and DNA barcoding of species from the Far. Zootaxa, 5636 (3), 499–510. https://doi.org/10.11646/zootaxa.5636.3.5
  34. Semenchenko, A.A., Cranston, P.S. & Makarchenko, E.A. (2024) A multi‑locus phylogeny for the Diamesinae (Chironomidae: Diptera) provides new insights into evolution of an amphitropical clade. Zoological Journal of the Linnean Society, 202, 1–16. https://doi.org/10.1093/zoolinnean/zlae035
  35. Tamura, K. & Nei, M. (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution, 10 (3), 512–526. https://doi.org/10.1093/oxfordjournals.molbev.a040023
  36. Tshernovskij, A.A. (1949) Key to larvae of the midges of the family Tendipedidae. In: Key to the USSR fauna, published by Zoological Institute of the USSR Academy of Sciences. Vol. 31. Nauka, Leningrad‑Moscow, pp. 1–185. [in Russian]
  37. Vuataz, L., Sartori, M., Wagner, A. & Monaghan, M.T. (2011) Toward a DNA taxonomy of Alpine Rhithrogena (Ephemeroptera, Heptageniidae) using a mixed Yule‑coalescent analysis of mitochondrial and nuclear DNA. PLoS One, 6 (5), e19728. https://doi.org/10.1371/journal.pone.0019728

How to Cite

Lods-Crozet, B., Semenchenko, A.A. & Makarchenko, E.A. (2025) Morphological redescription and DNA barcoding of Diamesa longipes Goetghebuer, 1941 (Diptera: Chironomidae: Diamesinae) from the Swiss Alps. Zootaxa, 5723 (1), 93–104. https://doi.org/10.11646/zootaxa.5723.1.4