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Type: Article
Published: 2026-01-28
Page range: 417-434
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New leafminers on Senegalia mellifera (formerly Acacia mellifera, Fabaceae) in Kenya (Lepidoptera: Nepticulidae & Cemiostomidae) are some of Africa’s smallest moths

Naturalis Biodiversity Center; PO Box 9517; 2300 RA Leiden; Netherlands
Insects Division; Natural History Museum; London SW7 5BD; United Kingdom
Lepidoptera Acacia Africa Rift Valley Acalyptris Leucoptera new synonymy new species new combination

Abstract

Two new species of leafminers, occurring on Senegalia mellifera (Vahl) Seigler & Ebinger in Kenya, are described: Acalyptris melliferae sp. nov. in Nepticulidae and Leucoptera aurantia sp. nov. in Cemiostomidae. Both were found in the Rift valley, near Lake Bogoria. These species represent the first known leafminers on the African ‘Acacia’ genus Senegalia. We also provide arguments from phylogenetic studies to consider Cemiostomidae as a separate family, rather than a subfamily in Lyonetiidae. A checklist of African species of Cemiostomidae is appended, the genera Crobylophora Meyrick, 1880 and Microthauma Walsingham, 1891 are synonymised with Leucoptera Hübner, 1825 and the following new combinations are proposed: Leucoptera byssinodes (Meyrick, 1914) comb. nov., L. speciosa (Ghesquière, 1940) comb. nov., and L. xanthochyta (Meyrick, 1918) comb. nov. from Crobylophora and L. metallifera (Walsingham, 1891) comb. nov. from Microthauma.

 

References

  1. Agassiz, D.J.L. (2009a) The Macrolepidoptera fauna of Acacia in the Kenyan Rift Valley (Part 2 - Description of new species). Tropical Lepidoptera Research, 19 (1), 9–17. [https://journals.flvc.org/troplep/article/view/90253]
  2. Agassiz, D.J.L. (2009b) The Macrolepidoptera fauna of Acacia in the Kenyan Rift Valley (Part 1). Tropical Lepidoptera Research, 19 (1), 4–8. [https://journals.flvc.org/troplep/article/view/90254]
  3. Agassiz, D.J.L. (2011) The Lepidoptera of Acacia domatia in Kenya, with description of two new genera and six new species. Journal of Natural History, 45 (29–30), 1867–1893. https://doi.org/10.1080/00222933.2011.565155
  4. Agassiz, D.J.L. & Aarvik, L. (2014) New Tortricidae (Lepidoptera) from East Africa with an account of the tortricid fauna of Acacia in the Kenyan Rift Valley. Zootaxa, 3861 (4), 369–97. https://doi.org/10.11646/zootaxa.3861.4.7
  5. Agassiz, D.J.L. & Bidzilya, O.V. (2016) Gelechiidae (Lepidoptera) bred from Acacia in Kenya with description of eight new species. Annals of the Ditsong National Museum of Natural History, 6 (07), 116–145. [https://hdl.handle.net/10520/EJC192280]
  6. Bairstow, K.A., Clarke, K.L., McGeoch, M.A. & Andrew, N.R. (2010) Leaf miner and plant galler species richness on Acacia: relative importance of plant traits and climate. Oecologia, 163 (2), 437–448. https://doi.org/10.1007/s00442-010-1606-4
  7. Bradley, J.D. (1958) Taxonomic notes on Leucoptera meyricki Ghesquière and Leucoptera coffeella (Guérin-Méneville) (Lepidoptera, Lyonetiidae). Bulletin of Entomological Research, 49 (03), 417–419. https://doi.org/10.1017/S0007485300053736
  8. Bradley, J.D. & Carter, D.J. (1982) A new lyonetiid moth, a pest of winged-bean. Systematic Entomology, 7 (1), 1–9. https://doi.org/10.1111/j.1365-3113.1982.tb00122.x
  9. Busck, A. (1902) Notes on North American Tineina. Journal of the New York Entomological Society, 10 (2), 89–100, pl. xii. [https://www.biodiversitylibrary.org/part/83666]
  10. Common, I.F.B. (1990) Moths of Australia. E. J. Brill, Leiden, 535 pp. https://doi.org/10.1071/9780643101227
  11. De Prins, J. & De Prins, W. (2011–2024a) Global Taxonomic Database of Gracillariidae (Lepidoptera). Belgian Biodiversity Platform. Available from: http://www.gracillariidae.net (accessed 27 September 2024)
  12. De Prins, J. & De Prins, W. (2011–2024b) Afromoths, online database of Afrotropical moth species (Lepidoptera). Belgian Biodiversity Platform, Brussels. Available from: http://www.afromoths.net/ (accessed 28 September 2024)
  13. De Prins, J. & Kawahara, A.Y. (2012) Systematics, revisionary taxonomy, and biodiversity of Afrotropical Lithocolletinae (Lepidoptera: Gracillariidae). Zootaxa, 3594 (1), 1–283. https://doi.org/10.11646/zootaxa.3594.1.1
  14. De Prins, J., Hartley, D., Sruoga, V., Nicholls, J., Wallace, J. & Zwick, A. (2025) Diversity of Australian Ornixolinae (Lepidoptera: Gracillariidae) with taxonomic and nomenclatural acts within the related taxa (Acrocercopinae and Gracillariinae) based on the evidence of museomics, bionomics, and mitogenomics. Zootaxa, 5616 (1), 1–340. https://doi.org/10.11646/zootaxa.5616.1.1
  15. Doorenweerd, C., Nieukerken, E.J. van & Menken, S.B.J. (2015) A global phylogeny of leafmining Ectoedemia moths (Lepidoptera: Nepticulidae): exploring host plant family shifts and allopatry as drivers of speciation. PLoS ONE, 10 (3), e0119586. https://doi.org/10.1371/journal.pone.0119586
  16. Doorenweerd, C., Nieukerken, E.J.van & Hoare, R.J.B. (2016) Phylogeny, classification and divergence times of pygmy leaf-mining moths (Lepidoptera: Nepticulidae): the earliest lepidopteran radiation on Angiosperms? Systematic Entomology, 42 (1), 267–287. https://doi.org/10.1111/syen.12212
  17. Fletcher, T.B. (1929) A list of the generic names used for Microlepidoptera. Memoirs of the Department of Agriculture in India, Entomological Series, 11, i–ix + 1–244.
  18. Ghesquière, J. (1940) Lépidoptères, Microlépidoptères. Premiere partie. Annales du Musée royal du Congo belge, Tervuren (Belgique), Serie 3, Catalogues raisonnés de la faune entomologique du Congo Belge. Annales du Musée du Congo Belge, C. Zoologie, Série III (II), 7 (1), 1–120, pls i–v.
  19. Grandi, G. (1931) Morfologia ed etologia comparata di insetti a regime specializzato. I. La morfologia comparata di alcuni stati larvali di 20 Microlepidotteri minatori. Bollettino del Laboratorio di Entomologia del Reale Istituto Superiore Agrario di Bologna, 4, 223–336.
  20. Green, D.S. (1984) A proposed origin of the Coffee leaf-miner, Leucoptera coffeella (Guerin-Meneville) (Lepidoptera: Lyonetiidae). Bulletin of the Entomological Society of America, 30 (1), 30–31. https://doi.org/10.1093/besa/30.1.30
  21. Heikkilä, M., Mutanen, M., Wahlberg, N., Sihvonen, P. & Kaila, L. (2015) Elusive ditrysian phylogeny: an account of combining systematized morphology with molecular data (Lepidoptera). BMC Evolutionary Biology, 15 (1), 260. https://doi.org/10.1186/s12862-015-0520-0
  22. Hering, M. (1933) Zwei neue Microlepidopteren aus Italien. Bollettino del Laboratorio di Entomologia del Reale Istituto Superiore Agrario di Bologna, 5, 104–108. [https://archive.bulletinofinsectology.org/pdfarticles/vol05-1932-33-104-108hering.pdf]
  23. Hübner, J. (1796–[1836]) Sammlung europäischer Schmetterlinge. Vol. 8. Tineae. s.n., Augsburg, 71 pls., 477 figs.
  24. Hübner, J. (1816–1826) Verzeichniss bekannter Schmettlinge. Bey dem Verfasser zu finden, Augsburg, 431 + 72 pp. https://doi.org/10.5962/bhl.title.48607
  25. Huemer, P. & Karsholt, O. (2018) Revision of the genus Megacraspedus Zeller, 1839, a challenging taxonomic tightrope of species delimitation (Lepidoptera, Gelechiidae). ZooKeys, 800. https://doi.org/10.3897/zookeys.800.26292
  26. Kawahara, A.Y., Plotkin, D., Espeland, M., Meusemann, K., Toussaint, E.F.A., Donath, A., Gimnich, F., Frandsen, P.B., Zwick, A., dos Reis, M., Barber, J.R., Peters, R.S., Liu, S., Zhou, X., Mayer, C., Podsiadlowski, L., Storer, C., Yack, J.E., Misof, B. & Breinholt, J.W. (2019) Phylogenomics reveals the evolutionary timing and pattern of butterflies and moths. Proceedings of the National Academy of Sciences, 116 (45), 22657–22663. https://doi.org/10.1073/pnas.1907847116
  27. Knölke, S., Erlacher, S., Hausmann, A., Miller, M.A. & Segerer, A.H. (2005) A procedure for combined genitalia dissection and DNA extraction in Lepidoptera. Insect Systematics and Evolution, 35 (4), 401–409. https://doi.org/10.1163/187631204788912463
  28. Kyalangalilwa, B., Boatwright, J.S., Daru, B.H., Maurin, O. & van der Bank, M. (2013) Phylogenetic position and revised classification of Acacia s.l. (Fabaceae: Mimosoideae) in Africa, including new combinations in Vachellia and Senegalia. Botanical Journal of the Linnean Society, 172 (4), 500–523. https://doi.org/10.1111/boj.12047
  29. Kyrki, J. (1984) The Yponomeutoidea: a reassessment of the superfamily and its suprageneric groups (Lepidoptera). Entomologica Scandinavica, 15 (1), 71–84. https://doi.org/10.1163/187631284X00064
  30. Lees, D.C. & Minet, J. (2022) Lepidoptera, Butterflies and Moths: Systematics and Diversity. In: Goodman, S.M. & Fisher, B.L. (Eds.), The new natural history of Madagascar. Princeton University Press, Princeton, pp. 1141–1172. https://doi.org/10.2307/j.ctv2ks6tbb.152
  31. Legrand, H. (1965) Lépidoptères des îles Seychelles et d’Aldabra. Mémoires du Musée National d’Histoire Naturelle, Série A, Zoologie, 37, 1–210, pls. i–xvi. [https://www.biodiversitylibrary.org/part/280353]
  32. Li, X., St Laurent, R., Earl, C., Doorenweerd, C., Nieukerken, E.J. van, Davis, D., Johns, C.A., Kawakita, A., Kobayashi, S., Zwick, A., Lopez-Vaamonde, C., Ohshima, I. & Kawahara, A.Y. (2021) Phylogeny of gracillariid leaf-mining moths: evolution of larval behaviour inferred from phylogenomic and Sanger data. Cladistics, 38 (3), 277–300. https://doi.org/10.1111/cla.12490
  33. Lopez-Vaamonde, C., Sire, L., Rasmussen, B., Rougerie, R., Wieser, C., Allaoui, A.A., Minet, J., deWaard, J.R., Decaëns, T. & Lees, D.C. (2018) DNA barcodes reveal deeply neglected diversity and numerous invasions of micromoths in Madagascar. Genome, 62 (3), 108–121. https://doi.org/10.1139/gen-2018-0065
  34. Mayer, C., Dietz, L., Call, E., Kukowka, S., Martin, S. & Espeland, M. (2021) Adding leaves to the Lepidoptera tree: capturing hundreds of nuclear genes from old museum specimens. Systematic Entomology, 46 (3), 649–671. https://doi.org/10.1111/syen.12481
  35. Mey, W. (1994) Taxonomische Bearbeitung der westpalaarktischen Arten der Gattung Leucoptera Hubner, (1825), s. l. (Lepidoptera, Lyonetiidae). Deutsche Entomologische Zeitschrift, 41 (1), 173–234. https://doi.org/10.1002/mmnd.19940410119
  36. Mey, W. (2020) Lyonetiidae and Bedelliidae (Yponomeutoidea). In: Mey, W. & Krüger, M. (Eds.), The Lepidoptera fauna of a crater valley in the Great Escarpment of South Africa: The Asante Sana Project, Esperiana Memoir, Delta Druck u. Verlag, Schwanfeld, pp. 207–211.
  37. Meyrick, E. (1880) Descriptions of Australian Micro-Lepidoptera. Proceedings of the Linnean Society of New South Wales, 5 (2), 132–182. https://doi.org/10.5962/bhl.part.15874
  38. Meyrick, E. (1918) Descriptions of South African Micro-Lepidoptera. Annals of the Transvaal Museum, 6 (2), 7–59. [https://www.biodiversitylibrary.org/part/358555]
  39. Meyrick, E. (1928) Exotic Microlepidoptera. Exotic Microlepidoptera, 3 (13), 385–416. [https://www.biodiversitylibrary.org/page/60297745]
  40. Mutanen, M., Wahlberg, N. & Kaila, L. (2010) Comprehensive gene and taxon coverage elucidates radiation patterns in moths and butterflies. Proceedings of the Royal Society B: Biological Sciences, 277, 2839–2848. https://doi.org/10.1098/rspb.2010.0392
  41. Nieukerken, E.J. van (2007) Acalyptris Meyrick: revision of the platani and staticis groups in Europe and the Mediterranean (Lepidoptera: Nepticulidae). Zootaxa, 1436 (1), 1–48. https://doi.org/10.11646/zootaxa.1436.1.1
  42. Nieukerken, E.J. van (2010) Order Lepidoptera, family Nepticulidae. In: Harten, A.van (Ed.), Arthropod Fauna of the United Arab Emirates. Vol. 3. Dar Al Ummah, Abu Dhabi, pp. 492–514. [http://www.repository.naturalis.nl/document/201468]
  43. Nieukerken, E.J. van (2020) Nepticulidae (Nepticuloidea). In: Mey, W. & Krüger, M. (Eds.), The Lepidoptera fauna of a crater valley in the Great Escarpment of South Africa: The Asante Sana Project. Esperiana Memoir. Delta Druck u. Verlag, Schwanfeld, pp. 49–84.
  44. Nieukerken, E.J. van & Geertsema, H. (2015) A new leafminer on grapevine and Rhoicissus (Vitaceae) in South Africa within an expanded generic concept of Holocacista (Insecta, Lepidoptera: Heliozelidae). ZooKeys, 507, 41–97. https://doi.org/10.3897/zookeys.507.9536
  45. Nieukerken, E.J. van, Doorenweerd, C., Stokvis, F.R. & Groenenberg, D.S.J. (2012) DNA barcoding of the leaf-mining moth subgenus Ectoedemia s. str. (Lepidoptera: Nepticulidae) with COI and EF1-α: two are better than one in recognising cryptic species. Contributions to Zoology, 81 (1), 1–24. https://doi.org/10.1163/18759866-08101001
  46. Nieukerken, E.J. van, Doorenweerd, C., Nishida, K. & Snyers, C. (2016a) New taxa, including three new genera show uniqueness of Neotropical Nepticulidae (Lepidoptera). ZooKeys, 628, 1–63. https://doi.org/10.3897/zookeys.628.9805
  47. Nieukerken, E.J. van, Doorenweerd, C., Hoare, R.J.B. & Davis, D.R. (2016b) Revised classification and catalogue of global Nepticulidae and Opostegidae (Lepidoptera: Nepticuloidea). ZooKeys, 628, 65–246. https://doi.org/10.3897/zookeys.628.9799
  48. Ratnasingham, S. & Hebert, P.D.N. (2007) BOLD: The Barcode of Life Data System (www.barcodinglife.org). Molecular Ecology Notes, 7 (3), 355–364. https://doi.org/10.1111/j.1471-8286.2007.01678.x
  49. Schell, T., Greve, C. & Podsiadlowski, L. (2025) Establishing genome sequencing and assembly for non-model and emerging model organisms: a brief guide. Frontiers in Zoology, 22 (1), 7. https://doi.org/10.1186/s12983-025-00561-7
  50. Scoble, M.J. (1980) The genus Niepeltia Strand: taxonomy, and comments on structure and relationships (Lepidoptera: Nepticulidae). Annals of the Transvaal Museum, 32 (8), 197–229. [https://journals.co.za/doi/10.10520/AJA00411752_178]
  51. Scoble, M.J. (1983) A revised cladistic classification of the Nepticulidae (Lepidoptera) with descriptions of new taxa mainly from South Africa. Transvaal Museum Monograph, 2, 1–105. [https://hdl.handle.net/10520/AJA090799001_83]
  52. Silvestri, F. (1943) Compendio di entomologia applicata (agraria - forestale - medica - veterinaria). Parte speciale. Vol. II. Bellavista, Portici, 512 pp.
  53. Sinclair, R.J. & Hughes, L. (2010) Leaf miners: The hidden herbivores. Austral Ecology, 35 (3), 300–313. https://doi.org/10.1111/j.1442-9993.2009.02039.x
  54. Sohn, J.-C., Regier, J.C., Mitter, C., Davis, D., Landry, J.-F., Zwick, A. & Cummings, M.P. (2013) A molecular phylogeny for Yponomeutoidea (Insecta, Lepidoptera, Ditrysia) and its implications for classification, biogeography and the evolution of host plant use. PLoS ONE, 8 (1), e55066. https://doi.org/10.1371/journal.pone.0055066
  55. Spencer, K.A. (1963) Notes on the African Agromyzidae (Diptera) - 4. Journal of the Entomological Society of Southern Africa, 26 (1), 94–124. [https://journals.co.za/doi/10.10520/AJA00128789_3010]
  56. Stewart, R.D., van der Bank, M. & Davies, T.J. (2024) Unveiling South African insect diversity: DNA barcoding’s contribution to biodiversity data. South African Journal of Science, 120 (5–6), 1–9. https://doi.org/10.17159/sajs.2024/16448
  57. Steyn, L., Geertsema, H. & Addison, P. (2021) The Bio-Ecology of the Grapevine Leaf Miner, Holocacista capensis (Lepidoptera: Heliozelidae) in the Western Cape, South Africa. African Entomology, 29 (1), 190–200. https://doi.org/10.4001/003.029.0190
  58. Stonis, J.R., Diškus, A., Remeikis, A., Vargas, H.A. & Solis, M.A. (2020a) Diagnostics and updated catalogue of Acalyptris Meyrick, the second largest genus of Nepticulidae (Lepidoptera) in the Americas. Zootaxa, 4748 (2), 201–247. https://doi.org/10.11646/zootaxa.4748.2.1
  59. Stonis, J.R., Remeikis, A., Diškus, A. & Navickaite, A. (2020b) Documenting new and little known leaf-mining Nepticulidae from middle and southwestern areas of the Asian continent. Zootaxa, 4881 (3), 401–452. https://doi.org/10.11646/zootaxa.4881.3.1
  60. Stonis, J.R., Remeikis, A., Diškus, A., Baryshnikova, S. & Solis, M.A. (2021) What are the smallest moths (Lepidoptera) in the world? Zootaxa, 4942 (2), 269–289. https://doi.org/10.11646/zootaxa.4942.2.8
  61. Stonis, J.R., Diškus, A., Remeikis, A. & Orlovskytė, S. (2025) Dvidulopsis gen. nov., a rare Neotropical genus of pygmy moths (Nepticulidae) endemic to lowland humid forests, a biome of conservation priority. Zootaxa, 5609 (4), 583–599. https://doi.org/10.11646/zootaxa.5609.4.8
  62. Vári, L. (1955) South African Lepidoptera 1. Descriptions of new leafmining Tineina. Annals of the Transvaal Museum, 22 (3), 331–351.
  63. Walsingham, L. (1914) Insecta Lepidoptera-Heterocera, 4. Tineina, Pterophorina, Orneodina and Pyralidina and Hepialina (part.). In: Godman, F.D. & Salvin, O. (Eds.), Biologia Centrali-Americana. Vol. 4. Dulau & co, London, pp. 281–392, pl. 10. [http://biodiversitylibrary.org/page/593456]
  64. Washbourn, R. (1940) On the distribution of Leucoptera daricella (Meyr.) with the description of a new leaf-miner from coffee. Bulletin of Entomological Research, 30 (4), 455–462. https://doi.org/10.1017/S0007485300040633
  65. White, O.W., Hall, A., Price, B.W., Williams, S.T. & Clark, M.D. (2025) A snakemake toolkit for the batch assembly, annotation and phylogenetic analysis of mitochondrial genomes and ribosomal genes from genome skims of museum collections. Molecular Ecology Resources, 25 (1), e14036. https://doi.org/10.1111/1755-0998.14036
  66. Wilkinson, C. (1979) A taxonomic study of the micro-lepidopteran genera Microcalyptris Braun and Fomoria Beirne occurring in the United States of America (Lepidoptera, Nepticulidae). Tijdschrift voor Entomologie, 122 (4), 59–90. [https://www.biodiversitylibrary.org/part/66351]
  67. Zeller, P.C. (1848) Die Gattungen der mit Augendeckeln versehenen blattminirenden Schaben. Linnaea Entomologica, 3, 248–344, pl. II in vol. 2. [https://www.biodiversitylibrary.org/page/44017229]

How to Cite

Nieukerken, E.J.V. & Agassiz, D.J.L. (2026) New leafminers on Senegalia mellifera (formerly Acacia mellifera, Fabaceae) in Kenya (Lepidoptera: Nepticulidae & Cemiostomidae) are some of Africa’s smallest moths. Zootaxa, 5752 (3), 417–434. https://doi.org/10.11646/zootaxa.5752.3.6