Abstract
The Eurasian Eagle Owl (Bubo bubo) exhibits complex taxonomic structure with multiple subspecies across its broad geographic range. While much is known about the species in Europe and parts of Asia, the taxonomic status and distribution of B. bubo subspecies in Iran remain poorly understood. Phylogenetic analysis based on partial Cytb sequences of 36 samples across Iran revealed two rather weakly diverged clades: a western Iranian clade clustering with European (mostly German) samples, and an eastern/southern Iranian clade clustering with Chinese samples. Genetic diversity indices for the Iranian population were relatively low, with three identified haplotypes and low nucleotide and haplotype diversity. The findings are consistent with the presence of two subspecies, B. bubo nikolskii and B. bubo interpositus, in Iran, aligning with recent taxonomic assessments, but further investigation with additional genetic markers is needed to clarify the deeper evolutionary relationships among these populations. This study has implications for conservation and future taxonomic revisions of Bubo bubo.
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