Abstract
Examination of the humpback red snapper, Lutjanus gibbus (Perciformes: Lutjanidae), from Lizard Island off the Great Barrier Reef and Rasdhoo Atoll, Maldives revealed the presence of a new species of Lobosorchis (Digenea: Cryptogonimidae), L. polygongylus n. sp. Lobosorchis polygongylus n. sp. is distinguished from the typeand only other species, L. tibaldiae by the combination of body size, oral spine number (60–81 in L. polygongylus, 47–56 in L. tibaldiae) and number of testes (13–25 in L. polygongylus, 9 in L. tibaldiae). Bayesian inference analysis using data from the internal transcribed spacers 1 and 2 (ITS1 and ITS2), 5.8S and the large subunit (LSU) nuclear ribosomal DNA of L. polygongylus, L. tibaldiae and species of Beluesca, Caulanus, Chelediadema, Neometadena, Latuterus, Retrovarium and Siphoderina was performed to explore phylogenetic relationships of species of Lobosorchis with other cryptogonimid taxa. Despite the significant morphological differences between Lobosorchis polygongylus and L. tibaldiae, these two species differed consistently by only 5 base pairs (bp) over the entire ITS region (3 bp in ITS1, 0 bp in 5.8S and 2 bp in ITS2) and 1 bp in the LSU rDNA regions examined. The ITS2 rDNA region was sequenced from metacercariae obtained from the fins, flesh or body cavities of a number of fishes belonging to the Blenniidae, Pomacentridae and Tetraodontidae and analysed using minimum evolution analysis with L. polygongylus and L. tibaldiae. This revealed the presence of two additional genotypes (putative Lobosorchis sp. A and B), which consistently differed from L. polygongylus by 1 and 4 bp, L. tibaldiae by 1 and 4 bp and from each other by 3 bp over the ITS2 dataset. Although these genetic differences are relatively small, when evaluated in light of the differences observed between L. polygongylus and L. tibaldiae (which are morphologically quite distinct) and differences seen in other congeneric cryptogonimid taxa, the ITS2 rDNA data alone suggest that at least two more species of Lobosorchis are present at Lizard Island. These data also suggest that the ITS2 rDNA region alone is suitable for resolving operational taxonomic units (OTUs) at the species level within the Cryptogonimidae based on what was observed in this and other cryptogonimid systems. A morphological description of metacercariae of L. tibaldiae obtained from two species of Pomacentridae at Heron Island, off the Great Barrier Reef is also provided.References
Agatsuma, T., Iwagami, M., Liu, C.X., Saitoh, Y., Kawanaka, M., Upatham, S., Qui, D. & Higuchi, T. (2001) Molecular phylogenetic position of Schistosoma sinensium in the genus Schistosoma. Journal of Helminthology, 75, 215–221.
Al-Yamani, F.Y. & Nahhas, F.M. (1981) Digenetic trematodes of marine fishes from the Kuwaiti Coast of the Arabian Gulf. Kuwait Bulletin of Marine Science, 3, 1–22.
Allen, G.R. (1985) FAO species catalogue. Vol. 6. Snappers of the world. An annotated and illustrated catalogue of lutjanid species known to date. Food and Agriculture Organization of the United Nations, Rome, 208 pp.
Anderson, G.R. & Barker, S.C. (1998) Inference of phylogeny and taxonomy within the Didymozoidae (Digenea) from the second internal transcribed spacer (ITS2) or ribosomal DNA. Systematic Parasitology, 41, 87–94.
Barber, P. & Boyce, S.L. (2006) Estimating diversity of Indo-Pacific coral reef stomatopods through DNA barcoding of stomatopod larvae. Proceedings of the Royal Society B, 273, 2053–2061.
Bell, A.S., Sommerville, C. & Valtonen, E.T. (2001) A molecular phylogeny of the genus Ichthyocotylurus (Digenea, Strigeidae). International Journal for Parasitology, 31, 833–842.
Bowles, J., Blair, D. & McManus, D.P. (1995) A molecular phylogeny of the human schistosomes. Molecular Phylogenetics and Evolution, 4, 103–109.
Bray, R.A. & Cribb, T.H. (1989) Digeneans of the family Opecoelidae Ozaki, 1925 from the southern Great Barrier Reef, including a new genus and three new species. Journal of Natural History, 23, 429–473.
Cribb, T.H. (1998) The diversity of the Digenea of Australian animals. International Journal for Parasitology, 28, 899–911.
Cribb, T.H., Anderson, G.R., Adlard, R.D. & Bray, R.A. (1998) A DNA-based demonstration of a three-host life-cycle for the Bivesiculidae (Platyhelminthes: Digenea). International Journal for Parasitology, 28, 1791–1795.
El-Naffar, M.K.I., Gobashy, A., El-Etreby, S.C. & Kardousha, M.M. (1992) General survey of helminth parasite genera of Arabian Gulf fishes (coasts of United Arab Emirates). Arab Gulf Journal of Scientific Research, 10, 99–110.
Fischthal, J.H. & Kuntz, R.E. (1964) Digenetic trematodes of fishes from Palawan Island, Philippines. Part II. Five Opecoelidae, including three new species. Proceedings of the Helminthological Society of Washington, 31, 40–46.
Froese, R. & Pauly, D. (2008) FishBase. World Wide Web electronic publication. Available from: http://www.fishbase.org (accessed 31 July 2008)
Hafeezullah, M. & Siddiqi, A.H. (1970) Digenetic trematodes of marine fishes of India. Part I. Bucephalidae and Cryptogonimidae. Indian Journal of Helminthology, 22, 1–22.
Knowlton, N. (1993) Sibling species in the sea. Annual Review of Ecology and Systematics, 24, 189–216.
Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin, F., Wallace, I.M., Wilm, A., Lopez, R., Thompson, J.D., Gibson, T.J. & Higgins, D.G. (2007) Clustal W and Clustal X version 2.0. Bioinformatics, 23, 2947–2948.
Layman, E.M. (1930) Parasitic worms from fishes of Peter the Great Bay. Izv Tikookeanskoi Nauchno-Promyslovoi Stantsii, Vladivostok, 3, 1–120.
Linton, E. (1910) Helminth fauna of the Dry Tortugas. II. Trematodes. Papers from the Tortugas Laboratory of the Carnegie Institute of Washington, 4, 11–98.
Looss, A. (1899) Weitere Beiträge zur Kenntnis der Trematoden fauna Aegyptens, zugleich Versuch einer natürlichen Gliederung des Genus Distomum Retzius. Zoologischer Jahrbücher, 12, 521–784.
Lühe M. (1906) Report on the trematode parasites from the marine fishes of Ceylon. The Royal Society Report on the Pearl Oyster Fisheries, 5, 97–108.
Maddison, D.R. & Maddison, W.P. (2005) MacClade 4: Analysis of phylogeny and character evolution. Version 4.08. Sinauer Associates, Sunderland, Massachusetts.
Madhavi, R. (1976) Digenetic trematodes from marine fishes of Waltair Coast, Bay of Bengal. Family Cryptogonimidae. Rivista di Parassitologia, 37, 313–321.
Miller, T.L. & Cribb, T.H. (2005) A new genus and species of cryptogonimid from Lutjanus spp. (Pisces: Lutjanidae) on the Great Barrier Reef and New Caledonia. Journal of Parasitology, 91, 922–924.
Miller, T.L. & Cribb, T.H. (2007a) Phylogenetic relationships of some common Indo-Pacific snappers (Perciformes: Lutjanidae) based on mitochondrial DNA sequences, with comments on the taxonomic position of the Caesioninae. Molecular Phylogenetics and Evolution, 44, 450–460.
Miller, T.L. & Cribb, T.H. (2007b) Coevolution of Retrovarium n. gen. (Digenea: Cryptogonimidae) in Lutjanidae and Haemulidae (Perciformes) in the Indo-West Pacific. International Journal for Parasitology, 37, 1023–1045.
Miller, T.L. & Cribb, T.H. (2007c) Two new cryptogonimid genera (Digenea: Cryptogonimidae) from Lutjanus bohar (Perciformes: Lutjanidae): analyses of multiple ribosomal DNA regions reveals wide geographic distribution and presence of cryptic species. Acta Parasitologica, 52, 104–113.
Miller, T.L. & Cribb, T.H. (2007d) Two new cryptogonimid genera Beluesca n. gen. and Chelediadema n. gen. (Digenea: Cryptogonimidae) from tropical Indo-West Pacific Haemulidae (Perciformes). Zootaxa, 1543, 45–60.
Miller, T.L. & Cribb, T.H. (2008) Family Cryptogonimidae Ward, 1917. In: Bray, R.A., Gibson, D.I. & Jones, A. (Eds.), Keys to the Trematoda. Vol.3. CAB International, Wallingford, pp. 51–112.
Morgan, J.A.T. & Blair, D. (1995) Nuclear rDNA ITS sequence variation in the trematode genus Echinostoma: an aid to establishing relationships within the 37-collar-spine group. Parasitology, 111, 609–615.
Nahhas, F.M., Sey, O. & Nishimoto, R. (1998) Digenetic trematodes of marine fishes from the Kuwaiti Coast of the Arabian Gulf: families Pleorchiidae, Fellodistomidae, and Cryptogonimidae, with a description of two new species, Neoparacryptogonimus sphericus and Paracryptogonimus ramadani. Journal of the Helminthological Society of Washington, 65, 129–140.
Nicoll, W. (1914) The trematode parasites of fishes from the English Channel. Journal of the Marine Biological Association of the United Kingdom, 10, 466–505.
Nolan, M.J. & Cribb, T.H. (2004) The life cycle of Paracardicoloides yamagutii Martin, 1974 (Digenea: Sanguinicolidae). Folia Parasitologica, 51, 320–326.
Nolan, M.J. & Cribb, T.H. (2005) The use and implications of ribosomal DNA sequencing for the discrimination of digenean species. Advances in Parasitology, 60, 101–163.
Odening, K. (1964) Zur Taxonomie der Trematodenordnung Plagiorchiata. Monatsberichte der Deutschen Akademie der Wissenschaften, 6, 191–198.
Odhner, T. (1902) Mitteilungen zur Kenntnis der Distomen II. Zentralblatt für Bakteriologie, Parasitenkunde und Infektionskrankheiten, 31, 152–162.
Ozaki, Y. (1925) Preliminary notes on a trematode with an anus. Journal of Parasitology, 12, 51–53.
Parukhin, A.M. (1970) [Study of the trematode fauna of fish in the Red Sea and Gulf of Aden] Biologiya Morya, Kiev, 20, 187–213. [in Russian]
Parukhin, A.M. (1971) [Study of the trematode fauna of fishes of the Red Sea and Gulf of Aden] Biologiya Morya, Kiev, 25, 136–146. [in Russian]
Poche, F. (1907) Einige Bermerkungen zur Nomenclatur der Trematoden. Zoologischer Anzeiger, 31, 124–126.
Posada, D. & Crandall, K.A. (1998) Modeltest: testing the model of DNA substitution. Bioinformatics, 14, 817–818.
Railliet, A. (1919) Nouveaux trematodes du chien, par Hall et Wigdor. Recueil de Médecine Vétérinaire, 95, 229–232.
Reaka-Kudla, M.L. (1997) The global biodiversity of coral reefs: a comparison with rain forests. In: Reaka-Kudla, M.L., Wilson, D.E. & Wilson, E.O. (Eds.), Biodiversity II: understanding and protecting our biological resources. Joseph Henry Press, Washington, DC, pp. 83–108.
Rigby, M.C., Lo, C.M., Cribb, T.H., Euzet, L., Faliex, E., Galzin, R., Holmes, J.C. & Morand, S. (1999) Checklist of the parasites of coral reef fishes from French Polynesia, with considerations on their potential role in these fish communities. Cybium, 23, 273–284.
Ronquist, F. & Huelsenbeck, J.P. (2003) MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574.
Rudolphi, C.A. (1819) Entozoorum synopsis cui accedunt mantissa duplex et indices loupletissimi. Sumptibus Augusti Rücker, Berlin, 811 pp.
Snyder, S.D. & Tkach, V.V. (2001) Phylogenetic and biogeographical relationships among some holarctic frog lung flukes (Digenea: Haematoloechidae). Journal of Parasitology, 87, 1433–1440.
Srivastava, H.D. (1939) Studies on the family Heterophyidae Odhner, 1914. Part III. Parasites belonging to a new subfamily Polyorchitreminae from an Indian fresh-water fish. Indian Journal of Veterinary Science and Animal Husbandry, 9, 165–168.
Stiles, C.W. & Hassall, A. (1898) Notes on parasites. 48. An inventory of the genera and subgenera of the trematode family Fasciolidae. Archives de Parasitologie, 1, 81–99.
Swofford, D.L. (2003) PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4.0b10. Sinauer Associates, Sunderland, Massachusetts.
Travassos, L. (1947) Contribuição ao conhecimento dos helmintos dos peixes d'agua doce do Brasil. II. (Trematoda, Heterophyidae). Memórias do Instituto Oswaldo Cruz, 45, 517–520.
Vigueras I.P. (1940) Prosogonotremidae n. fam. y Prosogonotrema bilabiatum n. gen., n. sp. (Trematoda, Distomata) parasito de Ocyurus chrysurus Bloch (Pisces). Memorias de la Sociedad Cubana de Historia Natural, 14, 249–252.
Yamaguti, S. (1934) Studies on the helminth fauna of Japan. Part 2. Trematodes of fishes, I. Japanese Journal of Zoology, 5, 249–541.
Yamaguti, S. (1942) Studies on the helminth fauna of Japan. Part 38. Larval trematodes of fishes. Japanese Journal of Medical Sciences, 2, 131–160.