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Type: Article
Published: 2009-04-01
Page range: 33–46
Abstract views: 116
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Anguina paludicola sp. n. (Tylenchida: Anguinidae): The nematode associated with Rathayibacter toxicus infection in Polypogon monspeliensis and Lachnagrostis filiformis in Australia

Plant and Soil Health, South Australian Research and Development Institute, GPO Box 397 Adelaide, SA, 5001, Australia Plant and Food Science, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, SA 5064, Australia Current address: Evolutionary Biology Unit, South Australian Museum, North Terrace, Adelaide, SA 5000, Australia
Plant and Food Science, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, SA 5064, Australia
Nematoda Anguinidae allozyme electrophoresis flood plain staggers morphology new species Australia

Abstract

Anguina paludicola sp. n. is described from Polypogon monspeliensis from southeastern South Australia and Lachnagrostis filiformis from northern New South Wales, following an examination of nematode populations by allozyme electrophoresis and morphological analysis. Genetic differences were consistent with those expected from conspecific allopatric populations and were based on geography rather than host. Morphological differences between nematodes from P. monspeliensis and L. filiformis were inconsistent and varied between different gall types on the same host plant. The new species is characterised by adults with irregular and variable tail termini, which appear lobed; females may have a process of vestigial ovary extending from the post uterine sac towards the tail; males are dorsally curved with a single reflexed testis; and the haploid chromosome complement is n=18.

References

  1. Allen, M.W. & Sher, S.A. (1967) Taxonomic problems concerning the phytoparasitic nematodes. Annual Review of Phytopathology, 5, 247–264.

    Bertozzi, T. & McKay, A.C. (1995) Incidence on Polypogon monspeliensis of Clavibacter toxicus and Anguina sp., the organisms associated with 'flood plain staggers' in South Australia. Australian Journal of Experimental Agriculture, 35, 567–569.

    Bertozzi, T. (2003) Biology and control of the anguinid nematode associated with flood plain staggers. PhD thesis, University of Adelaide, 247pp.

    Brzeski, M.W. (1981) The genera of Anguinidae (Nematoda, Tylenchida). Revue de Nématologie, 4, 2334.

    Davies, K.A. & Lloyd, J. (1996) Nematodes associated with Diptera in South Australia: A new species of Fergusobia Currie from a fergusoninid and a new record of Syrphonema Laumond & Lyon from a Syrphid. Transactions of the Royal Society of South Australia, 120, 13–20.

    Filipjev, I.N. & Schuurmans Stekhoven, J.H., Jr. (1941) A Manual of Agricultural Helminthology. Brill, Leiden, 847pp.

    Fisher, J.M., McKay, A.C. & Dubé, A.J. (1984) Observations on the growth of adults of Anguina funesta (Nematoda: Anguinidae). Nematologica, 30, 463–469.

    Krall, E.L. & Aomets, E.K. (1973) Cytological and genetical aspects of the evolution of Anguina, causing the formation of galls on grasses. In: Anon (Ed.), Materialy VI Pribaltiiskoi Nauchno-Koordinatsionnoi Konferentsii po Voprosam Parazitologii. Akademiia. Nauk Litovskoi SSR, Vilnius, pp. 82–85.

    McKay, A.C. & Ophel, K.M. (1993) Toxigenic Clavibacter/Anguina associations infecting grass seedheads. Annual Review of Phytopathology, 31, 151–167.

    McKay, A.C., Ophel, K.M., Reardon, T.B. & Gooden, J.M. (1993) Livestock deaths associated with Clavibacter toxicus/Anguina sp. infection in seedheads of Agrostis avenacea and Polypogon monspeliensis. Plant Disease, 77, 635–641.

    Nei, M. (1978). Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89, 583–590.

    Powers, T.O., Szalanski, A.L., Mullin, P.G., Harris, T.S., Bertozzi, T. & Greisbach, J.A. (2001) Identification of seed gall nematodes of agronomic and regulatory concern with PCR-RFLP of ITS1. Journal of Nematology, 33, 191–194.

    Richardson, B.J., Baverstock, P.R. & Adams, M. (1986) Allozyme Electrophoresis: A Handbook for Animal Systematics and Population Studies. Academic Press, Sydney, 410 pp.

    Riley, I.T., Reardon, T.B. & McKay, A.C. (1988) Electrophoretic resolution of species boundaries in seed-gall forming nematodes, Anguina spp. (Nematoda: Anguinidae), from some graminaceous hosts in Australia and New Zealand. Nematologica, 34, 401–411.

    Riley, I.T., Reardon, T.B. & Bertozzi, T. (1998) Allozyme analysis of Australian isolates of Dilophospora alopecuri. Mycological Research 102, 301–307.

    Rogers, J.S. (1972) Measures of genetic similarity and genetic distance. Studies in genetics VII. University of Texas Publication 7213, 145–153.

    Sneath, P.H.A. & Sokal, R.R. (1973) Numerical Taxonomy. W.H. Freeman, San Francisco, 588pp.

    Solov'eva, G.I. & Gruzdeva, L.I. (1977) Karyotypes of plant-parasitic nematodes of the genus Anguina Scopoli, 1799. Parazitologiya, 11, 366–368.

    Southey, J.F. (1973) Anguina agrostis. C.I.H Descriptions of Plant-parasitic Nematodes. Set 2, No. 20.Southey, J.F., Topham, P.B. & Brown, D.J.F. (1990) Taxonomy of some species of Anguina Scopoli, 1777 (sensu Brzeski, 1981) forming galls on gramineae: value of diagnostic characters and present status of nominal species. Revue de Nématologie, 13, 127–142.

    Stynes, B.A. & Bird, A.F. (1980a) Anguina agrostis, the vector of annual ryegrass toxicity in Australia. Nematologica, 26, 475–490.Stynes, B.A. & Bird, A.F. (1980b). Effects of methods of killing, fixing and mounting on measurements of Anguina agrostis. Nematologica, 26, 467–474.

    Subbotin, S.A., Krall, E.L., Riley, I.T., Chizhov, V.N., Staelens, A., de Loose, M. & Moens, M. (2004) Evolution of the gall forming plant-parasitic nematodes (Tylenchida:Anguinidae) and their relationships with hosts as inferred from Internal Transcribed Spacer sequenced of nuclear ribosomal DNA. Molecular Phylogenetics and Evolution, 30, 226–235.

    Thorne, G. (1949) On the classification of the Tylenchida, a new order (Nematoda, Phasmidia). Proceedings of the Helminthological Society of Washington, 16, 37–71.

    Triantaphyllou, A.C. (1971) Genetics and cytology. In: Zuckerman, B.M., Mai, W.F. & Rhode, R.A. (Eds.), Plant Parasitic Nematodes. Academic Press, New York and London, pp. 51–71.

    Triantaphyllou, A.C. & Hirschmann, H. (1966) Gametogenesis and reproduction in the wheat nematode, Anguina tritici. Nematologica, 12, 437–442.

    Triantaphyllou, A.C. & Hirschmann, H. (1980) Cytogenetics and morphology in relation to evolution and speciation of plant parasitic nematodes. Annual Review of Phytopathology, 18, 333–359.

    Tukey, J.W. (1953) The problem of multiple comparisons. In: Braun, H.I. (Ed.), The Collected Works of John W. Tukey: Vol. VIII Multiple Comparisons 1948–1983. Chapman and Hall, New York, 300 pp.