Abstract
We describe a new species of Hyalinobatrachium from the Amazonian slopes of the Andes in Peru and Bolivia on the basis of morphological, bioacoustic and genetic characteristics. Hyalinobatrachium carlesvilai sp. nov. can be distinguished from other species of Hyalinobatrachium by the combination of the following characters: (1) truncate snout in dorsal and lateral view; (2) white pericardium; (3) enameled dorsal, tarsal and cloacal folds; (4) hand webbing formula III 2 – – 1 + IV; (5) iris cream; (6) advertisement call consisting of a single, frequency-modulated note with a pulsed section followed by a tonal section. The new species had been previously identified as Hyalinobatrachium munozorum and H. bergeri. The advertisement call of the new species was previously assigned to H. bergeri. Here we describe the previously unknown call of Hyalinobatrachium bergeri. Additionally, we study the taxonomic status of H. lemur and H. pellucidum and place the former as synonym of the later. We extend the distribution of H. pellucidum to Departamento Cusco in southern Peru.References
Akaike, H. (1974) A new look at the statistical model identification. IEEE Transactions on Automatic Control, 19, 716–723.
AmphibiaWeb: Information on amphibian biology and conservation. [web application]. (2008) Berkeley, California: AmphibiaWeb. Available: http://amphibiaweb.org/. (Accessed: Dec 9, 2008).
Avise, J.C. (2000) Phylogeography. The history and formation of species. Cambridge: Harvard University Press.
Ayarzagüena, J. (1992) Los Centrolenidos de la Guayana Venezolana. Publicaciones de la Asociación de Amigos de Doñana, 1, 1–48.
Boersma, P. & Weenick, D. (2006) Praat: Doing Phonetics by Computer. Version 4.5.02. Free available software at http://www.fon.hum.uva.nl/praat/. Institute of Phonetic Sciences, University of Amsterdam, Amsterdam, The Netherlands.
Boettger, O. (1893) Ein neuer Laubfrosch aus Costa Rica. Berichte über die Senckenbergische naturforschende Gesellschaft in Frankfurt am Main, 1893, 251–252.
Cannatella, D.C. (1980) Two new species of Centrolenella from Bolivia (Anura: Centrolenidae). Proceedings of the Biological Society of Washington 93, 714–724.
Cannatella, D.C. & Duellman, W.E. (1982) Two new species of Centrolenella, with a brief review of the genus in Peru and Bolivia. Herpetologica, 38, 380–388.
Castroviejo-Fisher, S., Ayarzagüena, J. & Vilà, C. (2007) A new species of Hyalinobatrachium (Centrolenidae: Anura) from Serrania de Perijá, Venezuela. Zootaxa, 1441, 51–62.
Castroviejo-Fisher, S., Señaris, J.C., Ayarzagüena, J. & Vilà, C. (2008) Resurrection of Hyalinobatrachium orocostale and notes on the Hyalinobatrachium orientale species complex (Anura: Centrolenidae). Herpetologica, 64, 472–484.
Cisneros-Heredia, D.F. & McDiarmid, R.W. (2007) Revision of the characters of Centrolenidae (Amphibia: Anura: Athesphatanura), with comments on its taxonomy and the description of new taxa of glassfrogs. Zootaxa, 1572, 1–82.
Darst, C.R. & Cannatella, D.C. (2004) Novel relationships among hyloid frogs inferred from 12S and 16S mitochondrial DNA sequences. Molecular Phylogenetics and Evolution, 31, 462–475.
Dayrat, B. (2005) Toward integrative taxonomy. Biological Journal of the Linnean Society, 85, 407–415.
De la Riva, I. (2002) Hyalinobatrachium bergeri. Track 12, p. 11. In Márquez, R., De la Riva, I., Bosch, J. & Matheu, E. (Eds.), Sounds of frogs and toads of Bolivia. ALOSA-AHE-MNCN, Barcelona. 47 pp, 2 CDs, 166 recordings.
de Queiroz, K. (1998) The general lineage concept of species, species criteria, and the process of speciation: A conceptual unification and terminological recommendations. Pp. 57–75. In D. J. Howard and S. H. Berlocher (Eds.), Endless Forms: Species and Speciation. Oxford University Press, Oxford, U.K.
de Queiroz, K. (2005a) Ernst Mayr and the modern concept of species. Proceedings of the National Academy of Sciences, USA, 102, 6600–6607.
de Queiroz, K. (2005b) A unified concept of species and its consequences for the future of taxonomy. Proceedings of the California Academy of Sciences, 56 (Suppl. 1), 196–215.
de Queiroz, K. (2005c) Different species problems and their resolution. Bioessays, 27, 1263–1269.
de Queiroz, K. (2007) Species concepts and species delimitation. Systematic Biology, 56, 879–886.
Duellman, W.E. (1976) Centrolenids frogs from Perú. Occasional Papers of the Museum of Natural History, The University of Kansas, 52, 1–11.
Duellman, W.E. & Toft, C.A. (1979) Anurans from Serrania de Sira, Amazonian Peru: taxonomy and biogeography. Herpetologica, 35, 60–70.
Duellman, W.E. & Schulte, R. (1993) New species of centrolenid frogs from northern Peru. Occasional Papers of the Museum of Natural History, University of Kansas, 155, 1–33.
Duellman, W.E. & Señaris, J.C. (2003) A new species of glass frog (Anura: Centrolenidae) from the Venezuelan Guayana. Herpetologica, 59, 247–252.
Flores, G. (1985) A New Centrolenella (Anura) from Ecuador, with comments on nuptial pads and prepollical spines in Centrolenella. Journal of Herpetology, 19, 313–320.
Frost, D.R. (2009) Amphibian Species of the World: an Online Reference. Version 5.3 (12 February, 2009). Electronic Database accessible at http://research.amnh.org/herpetology/amphibia/index.php. American Museum of Natural History, New York, USA.
Frost, D. R., Grant, T., Faivovich, J., Bain, R. H., Haas, A., Haddad, C. F. B., de Sá, R. O., Channing, A., Wilkinson, M., Donnellan, S. C., Raxworthy, C. J., Campbell, J. A., Blotto, B. L., Moler, P., Drewes, R. C., Nussbaum, R. A., Lynch, J. D., Green, D. M. & Wheeler, W. C. (2006) The amphibian tree of life. Bulletin of the American Museum of Natural History, 297, 1–370.
Frost, D.R. & Kluge, A.G. (1994) A consideration of epistemology in systematic biology, with special reference to species. Cladistics, 10, 259–294.
Gene Codes Corporation. (2000) Sequencher Version 4.1. Gene Codes Corporation, Ann Arbor, Michigan, U.S.A.
Guayasamin, J.M., Bustamante, M.R., Almeida-Reinoso, D. & Funk, C.W. (2006) Glass frogs (Centrolenidae) of Yanayacu Biological Station, Ecuador, with the description of a new species and comments on centrolenid systematics. Zoological Journal of the Linnean Society of London, 147, 489–513.
Guayasamin, J.M., Castroviejo-Fisher, S., Ayarzagüena, J., Trueb, L. & Vilà, C. (2008a) Phylogenetic relationships of glassfrogs (Centrolenidae) based on mitochondrial and nuclear genes. Molecular Phylogenetics and Evolution, 48, 574–595.
Guayasamin, J.M., Castroviejo-Fisher, S., Trueb, L., Rada, M., & Vilà, C. (2009) Phylogenetic systematics of Glassfrogs (Amphibia: Centrolenidae) and their sister taxon Allophryne ruthveni. Zootaxa, 2100, 1–97.
Guayasamin, J.M., Cisneros-Heredia, D.F. & Castroviejo-Fisher, S. (2008b) Taxonomic identity of Cochranella petersi Goin, 1961 and Centrolenella ametarsia Flores, 1987. Zootaxa, 1815, 25–34.
Hillis, D.M., Moritz, C. & Mable, B.K. (1996) Molecular Systematics. Second edition. Sinauer Associates, Sunderland, MA, 655 pp.
Huelsenbeck, J.P. & Ronquist, F. (2001) MrBAYES: Bayesian inferences of phylogenetic trees. Bioinformatics, 8, 754–755.
Katoh, K., Kuma, K., Toh, H., & Miyata, T. (2005) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Research, 30, 3059–3066.
Knowles, L.L. & Carstens, B.C. (2007) Delimiting species without monophyletic gene trees. Systematic Biology, 56, 887–895.
Kok, P.J.R. & Castroviejo-Fisher, S. (2008) Glassfrogs (Anura: Centrolenidae) of Kaieteur National Park, Guyana, with notes on the distribution and taxonomy of some species of the family in the Guiana Shield. Zootaxa, 1680, 25–53.
Lynch, J.D. & Duellman, W.E. (1973) A review of the Centrolenid frogs of Ecuador, with descriptions of new species. The University of Kansas Museum of Natural History Occasional Papers, 16, 1–66.
Márquez, R., De la Riva, I. & Bosch, J. (1995) Advertisement calls of Bolivian Leptodactylidae (Amphibia, Anura). Journal of Zoology, 237, 313–336.
Márquez, R., De la Riva, I. & Bosch, J. (1996) Advertisement calls of three glass frogs from the Andean forests (Amphibia: Anura: Centrolenidae). Herpetological Journal, 6, 97–99.
Mayden, R.L. (1997) A hierarchy of species concepts: the denouement in the saga of the species problem. In: Claridge, M. F., Dawah, H. A., & Wilson, M. R. (Eds.) Species: the Units of Biodiversity (pp 381–424) London: Chapman & Hall Ltd.
Mayden R.L. (2002) On biological species, species concepts and individuation in the natural world. Fish and Fisheries, 3, 171–196.
Mazzoni, D. & Dannenberg R. (1999) Audacity: a free, cross-platform digital audio editor. Version 1.2.6. Free Software Foundation, Boston, Massachusetts, U.S.A.
Padial, J.M., Castroviejo-Fisher, S., Köhler, J., Vilá, C., Chaparro, J.C. & De la Riva, I. (2009) Deciphering the products of evolution at the species level: the need for an integrative taxonomy. Zoologica Scripta, 38, 431–447.
Posada, D. & Crandall, K.A. (1998) Modeltest: testing the model of DNA substitution. Bioinformatics, 14, 817–818.
Rannala, B. & Yang, Z. (1996) Probability distribution of molecular evolutionary trees: a new method of phylogenetic inference. Journal of Molecular Evolution, 43, 304–311.
Rodríguez, L., Martinez, J.L., Coloma, L.A., Ron, S., Cisneros-Heredia, D., Angulo, A. & Monteza, J.I. (2008) Hyalinobatrachium munozorum. IUCN 2008. 2006 IUCN Red List of Threatened Species. Available from http:// www.iucnredlist.org. (Accessed on 09 December 2008).
Ruiz-Carranza, P.M. & Lynch, J.D. (1991) Ranas Centrolenidae de Colombia I. Propuesta de una nueva clasificación genérica. Lozania, 57, 1–30.
Ruiz-Carranza, P.M. & Lynch, J.D. (1998) Ranas Centrolenidae de Colombia XI. Nuevas especies de ranas de cristal del genero Hyalinobatrachium. Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales 22, 571–586.
Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, NY, 1659 pp.
Savage, J.M. (1967) A New Tree-Frog (Centrolenidae) from Costa Rica. Copeia, 1967, 325–331.
Savage, J.M. & Heyer, W.R. (1967) Variation and distribution in the tree-frog genus Phyllomedusa. Beiträge zur Neotropischen Fauna, 2, 111–131.
Señaris, J.C. & Ayarzagüena, J. (2005) Revisión taxonómica de la Familia Centrolenidae (Amphibia; Anura) de Venezuela. Publicaciones del Comité Español del Programa Hombre y Biosfera – Red IberoMaB de la UNESCO, Sevilla, 337 pp.
Simpson, G.G. (1961) Principles of animal taxonomy. Columbia University Press, New York, U.S.A.
Swofford, D.L. (1998) PAUP*: Phylogenetic Analysis Using Parsimony (*and Other Methods), version 4.0b1, Sunderland, MA, Sinauer Associates.
Wiley, E.O. (1978) The evolutionary species concept reconsidered. Systematic Zoology, 27, 17–26.
Yang, Z. & Rannala, B. (1997) Bayesian phylogenetic inference using DNA sequences: a Markov Chain Monte Carlo method. Molecular Biology and Evolution, 14,717–724.