Abstract
Using a combination of mitochondrial and nuclear genetic markers, karyotypes and morphology, we examine the taxonomy of the Australo-papuan scincid lizard Carlia ‘fusca’ complex in northern Australia, all of which had been assigned previously to C. longipes. Carlia longipes, shows substantial variation in Y chromosome morphology between populations, indeed more than is seen between other species of Carlia. Analyses of the molecular genetic data and morphology demonstrate that populations with different Y chromosomes are two different species and also lead to the recognition of a third species from the Torres Strait. We herein define each of these species, for which previously described names can be applied.References
Anon (1985) Queensland Museum report of the board of trustees for the year 1884: presented to both Houses of Parliament by command. Pp 1–14.
Cavalli-Sforza, L.L. & Edwards, A.W.F. (1967) Phylogenetic analysis: models and estimation procedures. Evolution, 32, 550–570.
Cogger, H.G., Cameron E.E. & Cogger H.M. (1983) Zoological Catalogue of Australia. 1. Amphibia and Reptilia. Australian Government Printing Service, Canberra. Pp 313.
Couper, P.J., Worthington Wilmer, J., Roberts, L., Amey, A.P. & Zug, G.R. (2005) Skinks currently assigned to Carlia aerata (Scincidae: Lygosominae) of north-eastern Queensland: a preliminary study of cryptic diversity and two new species. Australian Journal of Zoology, 53, 35–49.
Covacevich, J. (1971) Amphibian and reptile type specimens in the Queensland Museum. Memoirs of the Queensland Museum, 16, 49–68.
De Vis, CW. (1985) A conspect of the genus Heteropus. Proceedings of the Royal Society of Queensland, 1, 166–173.
Dolman, G. & Hugall A.F. (2008) Combined mitochondrial and nuclear data enhance resolution of a rapid radiation of Australian rainbow skinks (Scincidae: Carlia). Molecular Phylogenetics and Evolution, 49, 782–794.
Donnellan, S.C. (1991) Chromosomes of Australian lygosomine skinks (Lacertilia: Scincidae). 1. The Egernia group: C-banding, Silver staining, Hoechst 33258 condensation analysis. Genetica, 83, 207–222.
Donnellan, S.C., Hutchinson, M.N. & Saint, K.M. (1999) Molecular evidence for the phylogeny of Australian gekkonoid lizards. Biological Journal of the Linnean Society, 67, 97–118.
Felsenstein, J. (1993) PHYLIP — Phylogeny Inference Package Version 3.5c.
Greer, A.E. (1975) Notes on the systematics of the genus Carlia (Lacertilia: Scincidae). I. Carlia melanopogon Gray 1845. Herpetologica, 31, 70–75.
Huson, D.H. (1998) SplitsTree-analysing and visualizing evolutionary data. Bioinformatics, 14, 68–73.
Ingram, G. & Covacevich, J.A. (1988) Revision of the genus Lygisaurus de Vis (Scincidae: Reptilia) in Australia. Memoirs of the Queensland Museum, 25, 335–354.
Ingram, G. & Covacevich, J.A. (1989) Revision of the genus Carlia (Reptilia, Scincidae) in Australia and comments on Carlia bicarinata of New Guinea. Memoirs of the Queensland Museum, 27, 443–490.
Kraus, F. (2007) Taxonomic partitioning within Papuan members of the Carlia novaeguineae complex (Squamata: Scincidae). Journal of Herpetology, 41, 410–423.
Macleay, W. (1877) The lizards of the Chevert Expedition. Proceedings of the Linnean Society of N.S.W., 2, 60–69.
Moussalli, A., Hugall, A.F. & Moritz, C. (2005) A mitochondrial phylogeny of the genus Saproscincus, Wells & Wellington (1984). Molecular Phylogenetics and Evolution, 34, 190–202.
Murphy, R.W., Sites, J.W., Buth, D.G. & Haufler, C.H. (1996) Proteins I: Isozyme Electrophoresis. In: Hillis D., Moritz, C., & Mable, B. (Eds.) Molecular Systematics. Sinauer, Sunderland, Massachusetts, pp. 51–120.
Posada, D. & Crandall, K.A. (1998) Modeltest: testing the model of DNA substitution. Bioinformatics, 14, 817–818.
Richardson, B.J., Baverstock, P.R. & Adams, M.A. (1986) Allozyme electrophoresis. Academic Press, Sydney, Australia.
Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574.
Sambrook, J., Fritsch, E.F. & Maniatis, T. (eds). (1989) Molecular Cloning, A Laboratory Manual 2nd Edition, Cold Spring Harbor Laboratory Press.
Stamatakis, A., Hoover, P. & J. Rougemont. (2008) A rapid bootstrap algorithm for the RAxML web-servers. Systematic Biology, 75, 758–771.
Stuart-Fox, D., Hugall, A. & Moritz, C. (2002) A molecular phylogeny of rainbow skinks (Scincidae: Carlia): taxonomic and biogeographic implications. Australian Journal of Zoology, 50, 39–51.
Sumner, A.T. (1972) A simple technique for demonstrating centromeric heterochromatin. Experimental Cell Research, 75, 304–6.
Swofford D.L. (1999) PAUP: Phylogenetic Analysis Using Parsimony, Version 4.0b3. Sinauer Associates, Inc. Publishers, Sunderland Massachusetts.
Swofford, D.L. & Selander, R.B. (1981) BIOSYS-1: A FORTRAN program for the comprehensive analysis of electrophoretic data in population genetics and systematics. Journal of Heredity, 72, 281–283.
Wells, R.W. & Wellington, C.R. (1984) A synopsis of the class Reptilia in Australia. Australian Journal of Herpetology, 1, 73–129.
Zug, G.R. (2004) Systematics of the Carlia "fusca" lizards (Squamata: Scincidae) of New Guinea and nearby islands. Bishop Museum Bulletin in Zoology, 5, i–viii+1–83.
Zug, G.R. & Allison, A. (2006) New Carlia fusca complex lizards (Reptilia: Squamata: Scincidae) from New Guinea, Papua-Indonesia. Zootaxa, 1237, 27–44.