Abstract
Inter-simple sequence repeat (ISSR)-PCR fingerprints are being increasingly used to establish relationships between closely related animals, although their inheritance patterns have not been examined closely as yet. To better understand the genetics of this relatively novel genomic fingerprint technique and to assess the tokogenetic information content of the data for the Hyles euphorbiae (Linnaeus, 1758) complex (HEC; Lepidoptera: Sphingidae) precisely, we examined five separated pairs of moths of Hyles euphorbiae from Spain (10 moths) and their offspring as an artificial population. ISSR-PCR fingerprints were obtained with four primers and the 0/1 matrix analysed. The cluster algorithm Neighbour Joining was successful in reconstructing the families as monophyletic, although intra-familial cluster formation cannot be represented by a dichotomous branching pattern. In contrast, the band sharing index did not detect significant differences in variability between siblings and individuals of H. euphorbiae from different localities compared at random. Rather high levels of recombination were detected, which surprisingly did not appear to obscure the tokogenetic signal to a significant degree. Thus ISSR-PCR fingerprints appear to be appropriate markers to study relationships between HEC individuals of two consecutive generations, while being too variable for higher levels.References
Assefa, K., Merker, A. & Tefera, H. (2003) Inter simple sequence repeat (ISSR) analysis of genetic diversity in tef [Eragrostis tef (Zucc.) Trotter]. Hereditas, 139, 174–183.
Bornet, B. & Branchard, M. (2001) Nonanchored Inter Simple Sequence Repeat (ISSR) markers: Reproducible and specific tools for genomic fingerprinting. Plant Molecular Biology Reports, 19, 209–215.
Culley, T.M. & Wolfe, A. (2001) Population genetic structure of the cleistogamous plant species Viola pubescens Aiton (Violaceae), as indicated by allozyme and ISSR molecular markers. Heredity, 86, 545–556.
Dice, L.R. (1945) Measures of the amount of ecologic association between species. Ecology, 26, 297–302.
Epplen, J.T., Melmer, G., Schmidt, P., Roewer, L., Hundrieser, J. & Epplen, C. (1992) On the potential of simple repetitive DNA for fingerprinting in clinical, forensic, and evolutionary dynamic studies. Clinical Investigation, 70, 1043–1051.
Fang, D.Q. & Roose, M.L. (1999) Inheritance of intersimple sequence repeats markers in Citrus. Journal of Heredity, 90, 247–249.
Gupta, M., Chyi, Y.-S., Romero-Severson, J. & Owen, J.L. (1994) Amplification of DNA markers from evolutionary diverse genomes using single primers of simple-sequence repeats. Theoretical and Applied Genetics, 89, 998–1006.
Hundsdoerfer, A.K., Kitching, I.J. & Wink, M. (2005a) A molecular phylogeny of the hawkmoth genus Hyles (Lepidoptera: Sphingidae, Macroglossinae). Molecular Phylogenetics and Evolution, 35, 442–458.
Hundsdoerfer, A.K., Kitching, I.J. & Wink, M. (2005b) The phylogeny of the Hyles euphorbiae-complex (Lepidoptera: Sphingidae): molecular evidence from sequence data and ISSR-PCR fingerprints. Organisms, Diversity and Evolution, 5, 173–198.
Hundsdoerfer, A.K. & Wink, M. (2005) New source of genetic polymorphisms in Lepidoptera? Zeitschrift fuer Naturforschung, 60c, 618–624.
Hundsdoerfer, A.K. & Wink, M. (2006) Incongruence of morphology and genetic markers in Hyles tithymali (Lepidoptera: Sphingidae) from the Canary Islands. Journal of Zoological Systematics and Evolutionary Research, 44, 316–322.
Jeffreys, A.J., Turner, M. & Debenham, P. (1991) The efficiency of multilocus DNA fingerprint probes for individualization and establishment of family relationships, determined from extensive casework. American Journal of Human Genetics, 48, 824–840.
Kol, N.V. & Lazebny, O.E. (2006) Polymorphism of ISSR-PCR markers in Tuvinian population of reindeer Rangifer tarandus L. Russian Journal of Genetics, 42, 1464–1466.
Martin, W. & Salamini, F. (2000) A meeting at the gene. Biodiversity and natural history. EMBO Reports, 1, 208–210.
Nagaraju, J., Kathirvel, M., Subbaiah, E.V., Muthulakshmi, M. & Kumar, L.D. (2002) FISSR-PCR: a simple and sensitive assay for highthroughput genotyping and genetic mapping. Molecular and Cellular Probes, 16, 67–72.
Nei, M. & Li, W.-H. (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences of the United States of America, 76, 5269–5273.
Perteguer, M.J., Rodríguez, E., García-Sánchez, R.N., Nogal-Ruiz, J.J., Bolas-Fernández, F., Martínez-Fernández, A.R. & Gárate, T. (2008) Identification of Spanish Trichinella isolates by ISSR-PCR: Intra-specific variability of Trichinella britovi. Vetenary Parasitology, doi: 10.1016/j.vetpar.2008.2008.10.062.
Paetkau, D., W. Calvert, I. Sterling, Strobeck, C. (1995) Microsatellite analysis of population structure in Canadian polar bears. Molecular Ecology, 4, 347–354.
Pradeep, A.R., Chatterjiee, S.N. & Nair, C.V. (2005) Genetic differentiation induced by selection in an inbred population of the silkworm Bombyx mori, revealed by RAPD and ISSR marker systems. Journal of Applied Genetics, 46, 291–298.
Swofford, D.L. (1998) PAUP*. Phylogenetic analysis using parsimony (*and other methods). Version 4.0b10. Sinauer Associates, Sunderland, Massachusetts, USA.
Zietkiewicz, E., Rafalski, A. & Labuda, D. (1994) Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics, 20, 176–183.